Subacute toxicity and endocrine-disrupting effects of Fe 2 O 3 , ZnO, and CeO 2 nanoparticles on amphibian metamorphosis

This study evaluated the potential toxic and endocrine-disrupting effects of sublethal concentrations of Fe O , CeO and ZnO nanoparticles (NPs) on amphibian metamorphosis. Tadpoles were exposed to several NPs concentrations, reaching a maximum of 1000 µg/L, for up to 21 days according to the amphibian metamorphosis assay (AMA). Some standard morphological parameters, such as developmental stage (DS), hind limb length (HLL), snout-to-vent length (SVL), wet body weight (WBW), and as well as post-exposure lethality were recorded in exposed organisms on days 7 and 21 of the bioassay. Furthermore, triiodothyronine (T3), thyroxine (T4) and malondialdehyde (MDA) levels and the activities of glutathione S-transferases (GST), glutathione reductase (GR), catalase (CAT), carboxylesterase (CaE), and acetylcholinesterase (AChE) were determined in exposed tadpoles as biomarkers. The results indicate that short-term exposure to Fe O NPs leads to toxic effects, both exposure periods cause toxic effects and growth inhibition for ZnO NPs, while short-term exposure to CeO NPs results in toxic effects and long-term exposure causes endocrine-disrupting effects. The responses observed after exposure to the tested NPs during amphibian metamorphosis suggest that they may have ecotoxicological effects and their effects should be monitored through field studies.