Epithelial Reactions to Hydroxyapatite: An in Vivo and in Vitro Study
The intention of this study was to investigate the epithelial reactions to hydroxyapatite ceramic in vivo and in vitro. Shortly after implantation in the rat middle ear, hydroxyapatite was found covered by a mucosal layer. In the early postoperative period the implant was almost completely covered b...
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| Veröffentlicht in: | Acta oto-laryngologica 1986, Vol.101 (3-4), p.231-241 |
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| creator | Van Blitterswijk, C. A. Kuijpers, W. Daems, W Th Grote, J. J. |
| description | The intention of this study was to investigate the epithelial reactions to hydroxyapatite ceramic in vivo and in vitro. Shortly after implantation in the rat middle ear, hydroxyapatite was found covered by a mucosal layer. In the early postoperative period the implant was almost completely covered by epithelial cells, which were found to proliferate and also showed migratory activity. After longer intervals the implant was completely covered by epithelium, which was composed predominantly of flat polygonal cells and a relatively small number of ciliated epithelium and goblet cells. All cells showed normal morphology. In vitro experiments showed preservation of the morphology of rat middle-ear mucosa explants with good outgrowth of epithelial cells. In these outgrows, the majority of the cells were flat polygonal, but ciliated epithelium was also seen. No difference was found between the absence and presence of hydroxyapatite. Serially cultured cells displayed normal polygonal morphology, but no ciliated cells were found. Ciliated cells were also absent in control experiments without hydroxyapatite. Growth curves obtained in the absence and presence of hydroxyapatite did not differ significantly from each other. |
| doi_str_mv | 10.3109/00016488609132832 |
| format | Article |
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A. ; Kuijpers, W. ; Daems, W Th ; Grote, J. J.</creator><creatorcontrib>Van Blitterswijk, C. A. ; Kuijpers, W. ; Daems, W Th ; Grote, J. J.</creatorcontrib><description>The intention of this study was to investigate the epithelial reactions to hydroxyapatite ceramic in vivo and in vitro. Shortly after implantation in the rat middle ear, hydroxyapatite was found covered by a mucosal layer. In the early postoperative period the implant was almost completely covered by epithelial cells, which were found to proliferate and also showed migratory activity. After longer intervals the implant was completely covered by epithelium, which was composed predominantly of flat polygonal cells and a relatively small number of ciliated epithelium and goblet cells. All cells showed normal morphology. In vitro experiments showed preservation of the morphology of rat middle-ear mucosa explants with good outgrowth of epithelial cells. In these outgrows, the majority of the cells were flat polygonal, but ciliated epithelium was also seen. No difference was found between the absence and presence of hydroxyapatite. Serially cultured cells displayed normal polygonal morphology, but no ciliated cells were found. Ciliated cells were also absent in control experiments without hydroxyapatite. Growth curves obtained in the absence and presence of hydroxyapatite did not differ significantly from each other.</description><identifier>ISSN: 0001-6489</identifier><identifier>EISSN: 1651-2251</identifier><identifier>DOI: 10.3109/00016488609132832</identifier><identifier>PMID: 3705952</identifier><identifier>CODEN: AOLAAJ</identifier><language>eng</language><publisher>Stockholm: Informa UK Ltd</publisher><subject>Animals ; Biological and medical sciences ; Cell Movement ; Cells, Cultured ; Ceramics - adverse effects ; Ear, Middle - drug effects ; Ear, Middle - surgery ; Ear, Middle - ultrastructure ; Epithelial Cells ; epithelium ; Epithelium - drug effects ; Epithelium - ultrastructure ; Head and neck surgery. Maxillofacial surgery. Dental surgery. Orthodontics ; hydroxyapatite ; Hydroxyapatites - adverse effects ; in vitro tests ; Male ; Medical sciences ; middle ear ; Mucous Membrane - cytology ; Mucous Membrane - drug effects ; Mucous Membrane - ultrastructure ; Prostheses and Implants - adverse effects ; rat ; Rats ; Rats, Inbred Strains ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Surgery of the ear, the auditive nerve and the facial nerve</subject><ispartof>Acta oto-laryngologica, 1986, Vol.101 (3-4), p.231-241</ispartof><rights>1986 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 1986</rights><rights>1986 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c345t-b6d9dd93abdcd4d263e651d2feb54003eb84f642dac1269694ce6d05d5515f63</citedby><cites>FETCH-LOGICAL-c345t-b6d9dd93abdcd4d263e651d2feb54003eb84f642dac1269694ce6d05d5515f63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.3109/00016488609132832$$EPDF$$P50$$Ginformahealthcare$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.3109/00016488609132832$$EHTML$$P50$$Ginformahealthcare$$H</linktohtml><link.rule.ids>314,780,784,4024,27923,27924,27925,59647,59753,60436,60542,61221,61256,61402,61437</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8715341$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3705952$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Van Blitterswijk, C. A.</creatorcontrib><creatorcontrib>Kuijpers, W.</creatorcontrib><creatorcontrib>Daems, W Th</creatorcontrib><creatorcontrib>Grote, J. J.</creatorcontrib><title>Epithelial Reactions to Hydroxyapatite: An in Vivo and in Vitro Study</title><title>Acta oto-laryngologica</title><addtitle>Acta Otolaryngol</addtitle><description>The intention of this study was to investigate the epithelial reactions to hydroxyapatite ceramic in vivo and in vitro. Shortly after implantation in the rat middle ear, hydroxyapatite was found covered by a mucosal layer. In the early postoperative period the implant was almost completely covered by epithelial cells, which were found to proliferate and also showed migratory activity. After longer intervals the implant was completely covered by epithelium, which was composed predominantly of flat polygonal cells and a relatively small number of ciliated epithelium and goblet cells. All cells showed normal morphology. In vitro experiments showed preservation of the morphology of rat middle-ear mucosa explants with good outgrowth of epithelial cells. In these outgrows, the majority of the cells were flat polygonal, but ciliated epithelium was also seen. No difference was found between the absence and presence of hydroxyapatite. Serially cultured cells displayed normal polygonal morphology, but no ciliated cells were found. Ciliated cells were also absent in control experiments without hydroxyapatite. Growth curves obtained in the absence and presence of hydroxyapatite did not differ significantly from each other.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Movement</subject><subject>Cells, Cultured</subject><subject>Ceramics - adverse effects</subject><subject>Ear, Middle - drug effects</subject><subject>Ear, Middle - surgery</subject><subject>Ear, Middle - ultrastructure</subject><subject>Epithelial Cells</subject><subject>epithelium</subject><subject>Epithelium - drug effects</subject><subject>Epithelium - ultrastructure</subject><subject>Head and neck surgery. Maxillofacial surgery. Dental surgery. Orthodontics</subject><subject>hydroxyapatite</subject><subject>Hydroxyapatites - adverse effects</subject><subject>in vitro tests</subject><subject>Male</subject><subject>Medical sciences</subject><subject>middle ear</subject><subject>Mucous Membrane - cytology</subject><subject>Mucous Membrane - drug effects</subject><subject>Mucous Membrane - ultrastructure</subject><subject>Prostheses and Implants - adverse effects</subject><subject>rat</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Surgery of the ear, the auditive nerve and the facial nerve</subject><issn>0001-6489</issn><issn>1651-2251</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE9LAzEQxYMotVY_gAchB6-r-bNJN-qllGqFgqDF65JNsjRlu1mSVN1v75atggg9zQzv_YaZB8AlRjcUI3GLEMI8zTKOBKYko-QIDDFnOCGE4WMw3OlJZxCn4CyE9W4UGRuAAR0jJhgZgtmssXFlKisr-GqkitbVAUYH56327quVjYw2mjs4qaGt4bv9cFDWuu-jd_AtbnV7Dk5KWQVzsa8jsHycLafzZPHy9DydLBJFUxaTgmuhtaCy0EqnmnBqumM1KU3BUoSoKbK05CnRUmHCBRepMlwjphnDrOR0BHC_VnkXgjdl3ni7kb7NMcp3geT_AumYq55ptsXG6F9in0CnX-91GZSsSi9rZcOvLRtjRlPc2R56m61L5zfy0_lK51G2lfM_DD10xf0ffGVkFVdKepOv3dbXXWgHfvgG0RGMmg</recordid><startdate>1986</startdate><enddate>1986</enddate><creator>Van Blitterswijk, C. A.</creator><creator>Kuijpers, W.</creator><creator>Daems, W Th</creator><creator>Grote, J. J.</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><general>Taylor and Francis</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>1986</creationdate><title>Epithelial Reactions to Hydroxyapatite: An in Vivo and in Vitro Study</title><author>Van Blitterswijk, C. A. ; Kuijpers, W. ; Daems, W Th ; Grote, J. J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c345t-b6d9dd93abdcd4d263e651d2feb54003eb84f642dac1269694ce6d05d5515f63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell Movement</topic><topic>Cells, Cultured</topic><topic>Ceramics - adverse effects</topic><topic>Ear, Middle - drug effects</topic><topic>Ear, Middle - surgery</topic><topic>Ear, Middle - ultrastructure</topic><topic>Epithelial Cells</topic><topic>epithelium</topic><topic>Epithelium - drug effects</topic><topic>Epithelium - ultrastructure</topic><topic>Head and neck surgery. Maxillofacial surgery. Dental surgery. Orthodontics</topic><topic>hydroxyapatite</topic><topic>Hydroxyapatites - adverse effects</topic><topic>in vitro tests</topic><topic>Male</topic><topic>Medical sciences</topic><topic>middle ear</topic><topic>Mucous Membrane - cytology</topic><topic>Mucous Membrane - drug effects</topic><topic>Mucous Membrane - ultrastructure</topic><topic>Prostheses and Implants - adverse effects</topic><topic>rat</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Surgery of the ear, the auditive nerve and the facial nerve</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Van Blitterswijk, C. A.</creatorcontrib><creatorcontrib>Kuijpers, W.</creatorcontrib><creatorcontrib>Daems, W Th</creatorcontrib><creatorcontrib>Grote, J. J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Acta oto-laryngologica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Van Blitterswijk, C. A.</au><au>Kuijpers, W.</au><au>Daems, W Th</au><au>Grote, J. J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Epithelial Reactions to Hydroxyapatite: An in Vivo and in Vitro Study</atitle><jtitle>Acta oto-laryngologica</jtitle><addtitle>Acta Otolaryngol</addtitle><date>1986</date><risdate>1986</risdate><volume>101</volume><issue>3-4</issue><spage>231</spage><epage>241</epage><pages>231-241</pages><issn>0001-6489</issn><eissn>1651-2251</eissn><coden>AOLAAJ</coden><abstract>The intention of this study was to investigate the epithelial reactions to hydroxyapatite ceramic in vivo and in vitro. Shortly after implantation in the rat middle ear, hydroxyapatite was found covered by a mucosal layer. In the early postoperative period the implant was almost completely covered by epithelial cells, which were found to proliferate and also showed migratory activity. After longer intervals the implant was completely covered by epithelium, which was composed predominantly of flat polygonal cells and a relatively small number of ciliated epithelium and goblet cells. All cells showed normal morphology. In vitro experiments showed preservation of the morphology of rat middle-ear mucosa explants with good outgrowth of epithelial cells. In these outgrows, the majority of the cells were flat polygonal, but ciliated epithelium was also seen. No difference was found between the absence and presence of hydroxyapatite. Serially cultured cells displayed normal polygonal morphology, but no ciliated cells were found. Ciliated cells were also absent in control experiments without hydroxyapatite. Growth curves obtained in the absence and presence of hydroxyapatite did not differ significantly from each other.</abstract><cop>Stockholm</cop><pub>Informa UK Ltd</pub><pmid>3705952</pmid><doi>10.3109/00016488609132832</doi><tpages>11</tpages></addata></record> |
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| ispartof | Acta oto-laryngologica, 1986, Vol.101 (3-4), p.231-241 |
| issn | 0001-6489 1651-2251 |
| language | eng |
| recordid | cdi_pubmed_primary_3705952 |
| source | MEDLINE; Taylor & Francis:Master (3349 titles); Taylor & Francis Medical Library - CRKN |
| subjects | Animals Biological and medical sciences Cell Movement Cells, Cultured Ceramics - adverse effects Ear, Middle - drug effects Ear, Middle - surgery Ear, Middle - ultrastructure Epithelial Cells epithelium Epithelium - drug effects Epithelium - ultrastructure Head and neck surgery. Maxillofacial surgery. Dental surgery. Orthodontics hydroxyapatite Hydroxyapatites - adverse effects in vitro tests Male Medical sciences middle ear Mucous Membrane - cytology Mucous Membrane - drug effects Mucous Membrane - ultrastructure Prostheses and Implants - adverse effects rat Rats Rats, Inbred Strains Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Surgery of the ear, the auditive nerve and the facial nerve |
| title | Epithelial Reactions to Hydroxyapatite: An in Vivo and in Vitro Study |
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