Resveratrol improves ovarian state by inhibiting apoptosis of granulosa cells
Among the natural polyphenolic compounds, resveratrol (RES) is known for reducing the effects of declining reproductive power through resisting senility, anti-oxidant and anti-inflammatory, while the molecular mechanism of RES in human ovaries is unclear. We aimed to evaluate the most likely mechani...
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| Veröffentlicht in: | Gynecological endocrinology 2023-12, Vol.39 (1), p.2181652-2181652 |
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| creator | Liang, Ying Xu, Mei-Ling Gao, Xing Wang, Yan Zhang, Li-Nan Li, Ya-Cong Guo, Qing |
| description | Among the natural polyphenolic compounds, resveratrol (RES) is known for reducing the effects of declining reproductive power through resisting senility, anti-oxidant and anti-inflammatory, while the molecular mechanism of RES in human ovaries is unclear. We aimed to evaluate the most likely mechanisms of RES against apoptosis induced by H
2
O
2
in human ovary granulosa cells.
Ovarian granulosa cells from infertile women (≤35 years old) were collected. Those patients defined as polycystic ovary syndrome (PCOS), poor ovarian responder (POR) and Endometriosis were excluded. Then they were randomly divided into control group, model group and the treatment group. Cellular apoptosis was analyzed by flow cytometer method. The related protein and mRNA expressions were detected by western blot and RT-PCR.
Apoptosis rates of the treatment group containing RES with concentrations of 1 μM and 10 μM were significantly decreased (p |
| doi_str_mv | 10.1080/09513590.2023.2181652 |
| format | Article |
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2
O
2
in human ovary granulosa cells.
Ovarian granulosa cells from infertile women (≤35 years old) were collected. Those patients defined as polycystic ovary syndrome (PCOS), poor ovarian responder (POR) and Endometriosis were excluded. Then they were randomly divided into control group, model group and the treatment group. Cellular apoptosis was analyzed by flow cytometer method. The related protein and mRNA expressions were detected by western blot and RT-PCR.
Apoptosis rates of the treatment group containing RES with concentrations of 1 μM and 10 μM were significantly decreased (p < 0.001). Western blot results demonstrated that the proteins levels of transforming growth factor-β (TGF-β), Bax and Caspase 9 were decreased, and Bcl-2 was increased under RES treatment, while the protein levels of Caspase 8, Caspase 3, growth differentiation factor 9 (GDF9) and bone morphogenetic protein 15 (BMP15) expressed no significant difference. The results by RT-PCR of follicle and ovarian development related mRNA factors were consistent with that of western blot assay.
In conclusion, the present study provides the evidence that RES may affects apoptotic factors to protect human ovarian state.</description><identifier>ISSN: 0951-3590</identifier><identifier>EISSN: 1473-0766</identifier><identifier>DOI: 10.1080/09513590.2023.2181652</identifier><identifier>PMID: 36824010</identifier><language>eng</language><publisher>England: Taylor & Francis</publisher><subject>Adult ; Apoptosis ; cell signaling ; Female ; Granulosa Cells - metabolism ; human ovary granulosa cells ; Humans ; Hydrogen Peroxide - metabolism ; Hydrogen Peroxide - pharmacology ; Infertility, Female - drug therapy ; Infertility, Female - metabolism ; Ovary - metabolism ; Polycystic Ovary Syndrome - metabolism ; resveratrol ; Resveratrol - pharmacology ; RNA, Messenger - metabolism</subject><ispartof>Gynecological endocrinology, 2023-12, Vol.39 (1), p.2181652-2181652</ispartof><rights>2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group 2023</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c479t-573ae0812cdc1e5cd057d274244fd8cf8ffef88c02d5e07aac801ce72b77d0523</citedby><cites>FETCH-LOGICAL-c479t-573ae0812cdc1e5cd057d274244fd8cf8ffef88c02d5e07aac801ce72b77d0523</cites><orcidid>0000-0003-1369-7495</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.1080/09513590.2023.2181652$$EPDF$$P50$$Ginformaworld$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.1080/09513590.2023.2181652$$EHTML$$P50$$Ginformaworld$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,864,2102,27502,27924,27925,59143,59144</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36824010$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liang, Ying</creatorcontrib><creatorcontrib>Xu, Mei-Ling</creatorcontrib><creatorcontrib>Gao, Xing</creatorcontrib><creatorcontrib>Wang, Yan</creatorcontrib><creatorcontrib>Zhang, Li-Nan</creatorcontrib><creatorcontrib>Li, Ya-Cong</creatorcontrib><creatorcontrib>Guo, Qing</creatorcontrib><title>Resveratrol improves ovarian state by inhibiting apoptosis of granulosa cells</title><title>Gynecological endocrinology</title><addtitle>Gynecol Endocrinol</addtitle><description>Among the natural polyphenolic compounds, resveratrol (RES) is known for reducing the effects of declining reproductive power through resisting senility, anti-oxidant and anti-inflammatory, while the molecular mechanism of RES in human ovaries is unclear. We aimed to evaluate the most likely mechanisms of RES against apoptosis induced by H
2
O
2
in human ovary granulosa cells.
Ovarian granulosa cells from infertile women (≤35 years old) were collected. Those patients defined as polycystic ovary syndrome (PCOS), poor ovarian responder (POR) and Endometriosis were excluded. Then they were randomly divided into control group, model group and the treatment group. Cellular apoptosis was analyzed by flow cytometer method. The related protein and mRNA expressions were detected by western blot and RT-PCR.
Apoptosis rates of the treatment group containing RES with concentrations of 1 μM and 10 μM were significantly decreased (p < 0.001). Western blot results demonstrated that the proteins levels of transforming growth factor-β (TGF-β), Bax and Caspase 9 were decreased, and Bcl-2 was increased under RES treatment, while the protein levels of Caspase 8, Caspase 3, growth differentiation factor 9 (GDF9) and bone morphogenetic protein 15 (BMP15) expressed no significant difference. The results by RT-PCR of follicle and ovarian development related mRNA factors were consistent with that of western blot assay.
In conclusion, the present study provides the evidence that RES may affects apoptotic factors to protect human ovarian state.</description><subject>Adult</subject><subject>Apoptosis</subject><subject>cell signaling</subject><subject>Female</subject><subject>Granulosa Cells - metabolism</subject><subject>human ovary granulosa cells</subject><subject>Humans</subject><subject>Hydrogen Peroxide - metabolism</subject><subject>Hydrogen Peroxide - pharmacology</subject><subject>Infertility, Female - drug therapy</subject><subject>Infertility, Female - metabolism</subject><subject>Ovary - metabolism</subject><subject>Polycystic Ovary Syndrome - metabolism</subject><subject>resveratrol</subject><subject>Resveratrol - pharmacology</subject><subject>RNA, Messenger - metabolism</subject><issn>0951-3590</issn><issn>1473-0766</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>0YH</sourceid><sourceid>EIF</sourceid><sourceid>DOA</sourceid><recordid>eNp9kU2P0zAQhi0EYsvCTwDlyCVl_BU7N9CKj5UWISE4WxNnXLxK4mKnRf33JLS7R06WrGfeVzMPY685bDlYeAet5lK3sBUg5FZwyxstnrANV0bWYJrmKdusTL1CV-xFKfcAXCojnrMr2VihgMOGff1O5UgZ55yGKo77nI5UqnTEHHGqyowzVd2pitOv2MU5TrsK92k_pxIXKlS7jNNhSAUrT8NQXrJnAYdCry7vNfv56eOPmy_13bfPtzcf7mqvTDvX2kgksFz43nPSvgdtemGUUCr01gcbAgVrPYheExhEb4F7MqIzZmGFvGa359w-4b3b5zhiPrmE0f37SHnnMM_RD-S8RWo95x58o6jVrQlWdR33PWprOlqy3p6zlt1_H6jMboxl3QYnSofihLEAjWwkX1B9Rn1OpWQKj9Uc3GrFPVhxqxV3sbLMvblUHLqR-sepBw0L8P4MxCmkPOKflIfezXgaUg7LiX0sTv6_4y_IeZzx</recordid><startdate>20231214</startdate><enddate>20231214</enddate><creator>Liang, Ying</creator><creator>Xu, Mei-Ling</creator><creator>Gao, Xing</creator><creator>Wang, Yan</creator><creator>Zhang, Li-Nan</creator><creator>Li, Ya-Cong</creator><creator>Guo, Qing</creator><general>Taylor & Francis</general><general>Taylor & Francis Group</general><scope>0YH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0003-1369-7495</orcidid></search><sort><creationdate>20231214</creationdate><title>Resveratrol improves ovarian state by inhibiting apoptosis of granulosa cells</title><author>Liang, Ying ; Xu, Mei-Ling ; Gao, Xing ; Wang, Yan ; Zhang, Li-Nan ; Li, Ya-Cong ; Guo, Qing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c479t-573ae0812cdc1e5cd057d274244fd8cf8ffef88c02d5e07aac801ce72b77d0523</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Adult</topic><topic>Apoptosis</topic><topic>cell signaling</topic><topic>Female</topic><topic>Granulosa Cells - metabolism</topic><topic>human ovary granulosa cells</topic><topic>Humans</topic><topic>Hydrogen Peroxide - metabolism</topic><topic>Hydrogen Peroxide - pharmacology</topic><topic>Infertility, Female - drug therapy</topic><topic>Infertility, Female - metabolism</topic><topic>Ovary - metabolism</topic><topic>Polycystic Ovary Syndrome - metabolism</topic><topic>resveratrol</topic><topic>Resveratrol - pharmacology</topic><topic>RNA, Messenger - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liang, Ying</creatorcontrib><creatorcontrib>Xu, Mei-Ling</creatorcontrib><creatorcontrib>Gao, Xing</creatorcontrib><creatorcontrib>Wang, Yan</creatorcontrib><creatorcontrib>Zhang, Li-Nan</creatorcontrib><creatorcontrib>Li, Ya-Cong</creatorcontrib><creatorcontrib>Guo, Qing</creatorcontrib><collection>Taylor & Francis Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Gynecological endocrinology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liang, Ying</au><au>Xu, Mei-Ling</au><au>Gao, Xing</au><au>Wang, Yan</au><au>Zhang, Li-Nan</au><au>Li, Ya-Cong</au><au>Guo, Qing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Resveratrol improves ovarian state by inhibiting apoptosis of granulosa cells</atitle><jtitle>Gynecological endocrinology</jtitle><addtitle>Gynecol Endocrinol</addtitle><date>2023-12-14</date><risdate>2023</risdate><volume>39</volume><issue>1</issue><spage>2181652</spage><epage>2181652</epage><pages>2181652-2181652</pages><issn>0951-3590</issn><eissn>1473-0766</eissn><abstract>Among the natural polyphenolic compounds, resveratrol (RES) is known for reducing the effects of declining reproductive power through resisting senility, anti-oxidant and anti-inflammatory, while the molecular mechanism of RES in human ovaries is unclear. We aimed to evaluate the most likely mechanisms of RES against apoptosis induced by H
2
O
2
in human ovary granulosa cells.
Ovarian granulosa cells from infertile women (≤35 years old) were collected. Those patients defined as polycystic ovary syndrome (PCOS), poor ovarian responder (POR) and Endometriosis were excluded. Then they were randomly divided into control group, model group and the treatment group. Cellular apoptosis was analyzed by flow cytometer method. The related protein and mRNA expressions were detected by western blot and RT-PCR.
Apoptosis rates of the treatment group containing RES with concentrations of 1 μM and 10 μM were significantly decreased (p < 0.001). Western blot results demonstrated that the proteins levels of transforming growth factor-β (TGF-β), Bax and Caspase 9 were decreased, and Bcl-2 was increased under RES treatment, while the protein levels of Caspase 8, Caspase 3, growth differentiation factor 9 (GDF9) and bone morphogenetic protein 15 (BMP15) expressed no significant difference. The results by RT-PCR of follicle and ovarian development related mRNA factors were consistent with that of western blot assay.
In conclusion, the present study provides the evidence that RES may affects apoptotic factors to protect human ovarian state.</abstract><cop>England</cop><pub>Taylor & Francis</pub><pmid>36824010</pmid><doi>10.1080/09513590.2023.2181652</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0003-1369-7495</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Adult Apoptosis cell signaling Female Granulosa Cells - metabolism human ovary granulosa cells Humans Hydrogen Peroxide - metabolism Hydrogen Peroxide - pharmacology Infertility, Female - drug therapy Infertility, Female - metabolism Ovary - metabolism Polycystic Ovary Syndrome - metabolism resveratrol Resveratrol - pharmacology RNA, Messenger - metabolism |
| title | Resveratrol improves ovarian state by inhibiting apoptosis of granulosa cells |
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