Supersensitive detection of single-histamine molecule on nanoplates by turn-on small molecule fluorescence sandwich immunoassay
We develop a supersensitive "turn-on format" fluorescence sandwich immunoassay for detecting small single molecules. Gold nanoplate-based biotin antibodies and streptavidin-fluorophores were used instead of streptavidin-horseradish peroxidase reacting with a biotin tracer in a microplate-b...
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| Veröffentlicht in: | Analyst (London) 2023-02, Vol.148 (4), p.714-718 |
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| container_title | Analyst (London) |
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| creator | Lee, Seungah Lee, Junghwa Kang, Seong Ho |
| description | We develop a supersensitive "turn-on format" fluorescence sandwich immunoassay for detecting small single molecules. Gold nanoplate-based biotin antibodies and streptavidin-fluorophores were used instead of streptavidin-horseradish peroxidase reacting with a biotin tracer in a microplate-based competitive enzyme-linked immunosorbent assay (ELISA). Our platform showed a low detection limit of 5 zeptomolar (5 × 10
-21
M), 5.4 × 10
10
times higher detection sensitivity than the conventional tune-off format ELISA.
Turn-on small molecule fluorescence sandwich immunoassay. |
| doi_str_mv | 10.1039/d2an01734b |
| format | Article |
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-21
M), 5.4 × 10
10
times higher detection sensitivity than the conventional tune-off format ELISA.
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-21
M), 5.4 × 10
10
times higher detection sensitivity than the conventional tune-off format ELISA.
Turn-on small molecule fluorescence sandwich immunoassay.</description><subject>Antibodies</subject><subject>Biotin</subject><subject>Chemical compounds</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Fluorescence</subject><subject>Fluorescent Dyes</subject><subject>Format</subject><subject>Histamine</subject><subject>Immunoassay</subject><subject>Peroxidase</subject><subject>Streptavidin</subject><issn>0003-2654</issn><issn>1364-5528</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpd0c9rFTEQB_Agin2tXrwrAS9F2Jrf2T3W-qtQ9KCel2x21qZkk2dmV3mn_utNfbUFTyEzH4YZvoS84OyEM9m9HYVLjFuphkdkw6VRjdaifUw2jDHZCKPVATlEvKpfzjR7Sg6kMZ1grdmQ62_rFgpCwrCE30BHWMAvISeaJ4oh_YzQXAZc3BwS0DlH8GsEWvvJpbyNbgGkw44ua0lNreLsYnxwU1xzAfSQPFB0afwT_CUN87ym7BDd7hl5MrmI8PzuPSI_Pn74fva5ufj66fzs9KLxwtil6VoNtuNOSMmFZVyKwdaKGo1quXQwdlxJMVkjmIVhGtpBdwqsn1rWdg64PCLH-7nbkn-tgEs_h7pWjC5BXrEX1nTSaqHaSl__R69yva5uV5XVhiltbtWbvfIlIxaY-m0Jsyu7nrP-Npb-vTj98jeWdxW_uhu5DjOM9_RfDhW83IOC_r77kKu8AcDPk2U</recordid><startdate>20230213</startdate><enddate>20230213</enddate><creator>Lee, Seungah</creator><creator>Lee, Junghwa</creator><creator>Kang, Seong Ho</creator><general>Royal Society of Chemistry</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-2101-4113</orcidid></search><sort><creationdate>20230213</creationdate><title>Supersensitive detection of single-histamine molecule on nanoplates by turn-on small molecule fluorescence sandwich immunoassay</title><author>Lee, Seungah ; Lee, Junghwa ; Kang, Seong Ho</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c267t-985e791a2331270132b75e74d64813aed91432f76207ebfb8b594e7cf8089ae13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Antibodies</topic><topic>Biotin</topic><topic>Chemical compounds</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Fluorescence</topic><topic>Fluorescent Dyes</topic><topic>Format</topic><topic>Histamine</topic><topic>Immunoassay</topic><topic>Peroxidase</topic><topic>Streptavidin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, Seungah</creatorcontrib><creatorcontrib>Lee, Junghwa</creatorcontrib><creatorcontrib>Kang, Seong Ho</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Analyst (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, Seungah</au><au>Lee, Junghwa</au><au>Kang, Seong Ho</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Supersensitive detection of single-histamine molecule on nanoplates by turn-on small molecule fluorescence sandwich immunoassay</atitle><jtitle>Analyst (London)</jtitle><addtitle>Analyst</addtitle><date>2023-02-13</date><risdate>2023</risdate><volume>148</volume><issue>4</issue><spage>714</spage><epage>718</epage><pages>714-718</pages><issn>0003-2654</issn><eissn>1364-5528</eissn><abstract>We develop a supersensitive "turn-on format" fluorescence sandwich immunoassay for detecting small single molecules. Gold nanoplate-based biotin antibodies and streptavidin-fluorophores were used instead of streptavidin-horseradish peroxidase reacting with a biotin tracer in a microplate-based competitive enzyme-linked immunosorbent assay (ELISA). Our platform showed a low detection limit of 5 zeptomolar (5 × 10
-21
M), 5.4 × 10
10
times higher detection sensitivity than the conventional tune-off format ELISA.
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| fulltext | fulltext |
| identifier | ISSN: 0003-2654 |
| ispartof | Analyst (London), 2023-02, Vol.148 (4), p.714-718 |
| issn | 0003-2654 1364-5528 |
| language | eng |
| recordid | cdi_pubmed_primary_36692086 |
| source | MEDLINE; Royal Society of Chemistry Journals Archive (1841-2007); Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Antibodies Biotin Chemical compounds Enzyme-Linked Immunosorbent Assay Fluorescence Fluorescent Dyes Format Histamine Immunoassay Peroxidase Streptavidin |
| title | Supersensitive detection of single-histamine molecule on nanoplates by turn-on small molecule fluorescence sandwich immunoassay |
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