Generation of a new human induced pluripotent stem cell (hiPSC) line from a South Asian Indian with a MYBPC3 Δ 25bp variant

Generation of a new human induced pluripotent stem cell (hiPSC) line from a South Asian Indian with a MYBPC3 Δ 25bp variant

Myosin binding protein C3 (MYBPC3) is a thick filament contractile protein that interacts with myosin, titin and actin and regulates cardiac muscle contraction. Genetic variations in the MYBPC3 gene are known causal factors for cardiomyopathy and heart failure. Previously, we identified a recurrent...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Stem cell research 2022-12, Vol.65, p.102978
Hauptverfasser: Chimata, Prasanth, Kashyap, Deepak K, Sairam, Thiagarajan, Ganesh, Akshayaa, Thangaraj, Kumarasamy, Purushottam, Meera, Viswanath, Biju, Jain, Sanjeev, Dhandapany, Perundurai S
Format: Artikel
Sprache:eng
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page
container_issue
container_start_page 102978
container_title Stem cell research
container_volume 65
creator Chimata, Prasanth
Kashyap, Deepak K
Sairam, Thiagarajan
Ganesh, Akshayaa
Thangaraj, Kumarasamy
Purushottam, Meera
Viswanath, Biju
Jain, Sanjeev
Dhandapany, Perundurai S
description Myosin binding protein C3 (MYBPC3) is a thick filament contractile protein that interacts with myosin, titin and actin and regulates cardiac muscle contraction. Genetic variations in the MYBPC3 gene are known causal factors for cardiomyopathy and heart failure. Previously, we identified a recurrent MYBPC3 deletion (25 base pairs) among South Asians associated with cardiomyopathy and heart failure. Here, we generated an induced pluripotent stem cell (iPSC) line using peripheral blood mononuclear cells (PBMC) from an Indian harboring MYBPC3 deletion. This iPSC line displayed embryonic stem cell morphology, expressed pluripotency markers, differentiated into three germ layers and exhibited normal karyotype.
format Article
fullrecord <record><control><sourceid>pubmed</sourceid><recordid>TN_cdi_pubmed_primary_36403549</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>36403549</sourcerecordid><originalsourceid>FETCH-pubmed_primary_364035493</originalsourceid><addsrcrecordid>eNqFjstqwkAYhQeh1Nj6CvIv24WQZkxilhqqdSEIunElY_KHjMyNuVQEH6PP5TOZQl139cE554PTI9HHNM_GeZ7SPhk4d4rjtEimyTPp02wS03RSROS6RIWWea4V6AYYKDxDGyRTwFUdKqzBiGC50R6VB-dRQoVCwFvLN9vyHQRXCI3VsnO3OvgWZo539krVvzjzLmGw3s83JYXbDyTp0cA3s13pX8lTw4TD4R9fyGjxuSu_xiYcJdYHY7lk9nJ43KX_Du6s6Ep6</addsrcrecordid><sourcetype>Index Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Generation of a new human induced pluripotent stem cell (hiPSC) line from a South Asian Indian with a MYBPC3 Δ 25bp variant</title><source>Elsevier ScienceDirect Journals Complete</source><source>DOAJ Directory of Open Access Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Chimata, Prasanth ; Kashyap, Deepak K ; Sairam, Thiagarajan ; Ganesh, Akshayaa ; Thangaraj, Kumarasamy ; Purushottam, Meera ; Viswanath, Biju ; Jain, Sanjeev ; Dhandapany, Perundurai S</creator><creatorcontrib>Chimata, Prasanth ; Kashyap, Deepak K ; Sairam, Thiagarajan ; Ganesh, Akshayaa ; Thangaraj, Kumarasamy ; Purushottam, Meera ; Viswanath, Biju ; Jain, Sanjeev ; Dhandapany, Perundurai S</creatorcontrib><description>Myosin binding protein C3 (MYBPC3) is a thick filament contractile protein that interacts with myosin, titin and actin and regulates cardiac muscle contraction. Genetic variations in the MYBPC3 gene are known causal factors for cardiomyopathy and heart failure. Previously, we identified a recurrent MYBPC3 deletion (25 base pairs) among South Asians associated with cardiomyopathy and heart failure. Here, we generated an induced pluripotent stem cell (iPSC) line using peripheral blood mononuclear cells (PBMC) from an Indian harboring MYBPC3 deletion. This iPSC line displayed embryonic stem cell morphology, expressed pluripotency markers, differentiated into three germ layers and exhibited normal karyotype.</description><identifier>EISSN: 1876-7753</identifier><identifier>PMID: 36403549</identifier><language>eng</language><publisher>England</publisher><ispartof>Stem cell research, 2022-12, Vol.65, p.102978</ispartof><rights>Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36403549$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chimata, Prasanth</creatorcontrib><creatorcontrib>Kashyap, Deepak K</creatorcontrib><creatorcontrib>Sairam, Thiagarajan</creatorcontrib><creatorcontrib>Ganesh, Akshayaa</creatorcontrib><creatorcontrib>Thangaraj, Kumarasamy</creatorcontrib><creatorcontrib>Purushottam, Meera</creatorcontrib><creatorcontrib>Viswanath, Biju</creatorcontrib><creatorcontrib>Jain, Sanjeev</creatorcontrib><creatorcontrib>Dhandapany, Perundurai S</creatorcontrib><title>Generation of a new human induced pluripotent stem cell (hiPSC) line from a South Asian Indian with a MYBPC3 Δ 25bp variant</title><title>Stem cell research</title><addtitle>Stem Cell Res</addtitle><description>Myosin binding protein C3 (MYBPC3) is a thick filament contractile protein that interacts with myosin, titin and actin and regulates cardiac muscle contraction. Genetic variations in the MYBPC3 gene are known causal factors for cardiomyopathy and heart failure. Previously, we identified a recurrent MYBPC3 deletion (25 base pairs) among South Asians associated with cardiomyopathy and heart failure. Here, we generated an induced pluripotent stem cell (iPSC) line using peripheral blood mononuclear cells (PBMC) from an Indian harboring MYBPC3 deletion. This iPSC line displayed embryonic stem cell morphology, expressed pluripotency markers, differentiated into three germ layers and exhibited normal karyotype.</description><issn>1876-7753</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNqFjstqwkAYhQeh1Nj6CvIv24WQZkxilhqqdSEIunElY_KHjMyNuVQEH6PP5TOZQl139cE554PTI9HHNM_GeZ7SPhk4d4rjtEimyTPp02wS03RSROS6RIWWea4V6AYYKDxDGyRTwFUdKqzBiGC50R6VB-dRQoVCwFvLN9vyHQRXCI3VsnO3OvgWZo539krVvzjzLmGw3s83JYXbDyTp0cA3s13pX8lTw4TD4R9fyGjxuSu_xiYcJdYHY7lk9nJ43KX_Du6s6Ep6</recordid><startdate>202212</startdate><enddate>202212</enddate><creator>Chimata, Prasanth</creator><creator>Kashyap, Deepak K</creator><creator>Sairam, Thiagarajan</creator><creator>Ganesh, Akshayaa</creator><creator>Thangaraj, Kumarasamy</creator><creator>Purushottam, Meera</creator><creator>Viswanath, Biju</creator><creator>Jain, Sanjeev</creator><creator>Dhandapany, Perundurai S</creator><scope>NPM</scope></search><sort><creationdate>202212</creationdate><title>Generation of a new human induced pluripotent stem cell (hiPSC) line from a South Asian Indian with a MYBPC3 Δ 25bp variant</title><author>Chimata, Prasanth ; Kashyap, Deepak K ; Sairam, Thiagarajan ; Ganesh, Akshayaa ; Thangaraj, Kumarasamy ; Purushottam, Meera ; Viswanath, Biju ; Jain, Sanjeev ; Dhandapany, Perundurai S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-pubmed_primary_364035493</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chimata, Prasanth</creatorcontrib><creatorcontrib>Kashyap, Deepak K</creatorcontrib><creatorcontrib>Sairam, Thiagarajan</creatorcontrib><creatorcontrib>Ganesh, Akshayaa</creatorcontrib><creatorcontrib>Thangaraj, Kumarasamy</creatorcontrib><creatorcontrib>Purushottam, Meera</creatorcontrib><creatorcontrib>Viswanath, Biju</creatorcontrib><creatorcontrib>Jain, Sanjeev</creatorcontrib><creatorcontrib>Dhandapany, Perundurai S</creatorcontrib><collection>PubMed</collection><jtitle>Stem cell research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chimata, Prasanth</au><au>Kashyap, Deepak K</au><au>Sairam, Thiagarajan</au><au>Ganesh, Akshayaa</au><au>Thangaraj, Kumarasamy</au><au>Purushottam, Meera</au><au>Viswanath, Biju</au><au>Jain, Sanjeev</au><au>Dhandapany, Perundurai S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Generation of a new human induced pluripotent stem cell (hiPSC) line from a South Asian Indian with a MYBPC3 Δ 25bp variant</atitle><jtitle>Stem cell research</jtitle><addtitle>Stem Cell Res</addtitle><date>2022-12</date><risdate>2022</risdate><volume>65</volume><spage>102978</spage><pages>102978-</pages><eissn>1876-7753</eissn><abstract>Myosin binding protein C3 (MYBPC3) is a thick filament contractile protein that interacts with myosin, titin and actin and regulates cardiac muscle contraction. Genetic variations in the MYBPC3 gene are known causal factors for cardiomyopathy and heart failure. Previously, we identified a recurrent MYBPC3 deletion (25 base pairs) among South Asians associated with cardiomyopathy and heart failure. Here, we generated an induced pluripotent stem cell (iPSC) line using peripheral blood mononuclear cells (PBMC) from an Indian harboring MYBPC3 deletion. This iPSC line displayed embryonic stem cell morphology, expressed pluripotency markers, differentiated into three germ layers and exhibited normal karyotype.</abstract><cop>England</cop><pmid>36403549</pmid></addata></record>
fulltext fulltext
identifier EISSN: 1876-7753
ispartof Stem cell research, 2022-12, Vol.65, p.102978
issn 1876-7753
language eng
recordid cdi_pubmed_primary_36403549
source Elsevier ScienceDirect Journals Complete; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals
title Generation of a new human induced pluripotent stem cell (hiPSC) line from a South Asian Indian with a MYBPC3 Δ 25bp variant
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T03%3A11%3A20IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-pubmed&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Generation%20of%20a%20new%20human%20induced%20pluripotent%20stem%20cell%20(hiPSC)%20line%20from%20a%20South%20Asian%20Indian%20with%20a%20MYBPC3%20%CE%94%2025bp%20variant&rft.jtitle=Stem%20cell%20research&rft.au=Chimata,%20Prasanth&rft.date=2022-12&rft.volume=65&rft.spage=102978&rft.pages=102978-&rft.eissn=1876-7753&rft_id=info:doi/&rft_dat=%3Cpubmed%3E36403549%3C/pubmed%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/36403549&rfr_iscdi=true