The effects of crocetin on oxidative stress induced ARPE-19 cells by H 2 O 2
The anti-apoptotic and antioxidant effects of crocetin was aimed to investigate on the oxidative damage model of ARPE-19 cells. The oxidative damage in ARPE cells was developed by H O treatment at 800 μM. Different doses of crocetin (1-80 μM) were applied for 24 h, and the effects on viability were...
Gespeichert in:
| Veröffentlicht in: | Experimental eye research 2023-01, Vol.226, p.109305 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | 109305 |
| container_title | Experimental eye research |
| container_volume | 226 |
| creator | Demirci Kucuk, Kübra Tokuc, Ecem Onder Aciksari, Aysegul Duruksu, Gokhan Yazir, Yusufhan Karabas, Veysel Levent |
| description | The anti-apoptotic and antioxidant effects of crocetin was aimed to investigate on the oxidative damage model of ARPE-19 cells. The oxidative damage in ARPE cells was developed by H
O
treatment at 800 μM. Different doses of crocetin (1-80 μM) were applied for 24 h, and the effects on viability were evaluated to find out the optimum drug dose. At first, three effective doses of crocetin (10, 20, 40 μM) on cell viability were selected for further analyses. The levels of superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione (GSH) were determined, and the expression of pro-apoptotic Bax gene and anti-apoptotic Bcl-2 gene were evaluated. The most effective crocetin dose on cell viability was found to be 10 μM. After the H
O
treatment, SOD and GSH were decreased and MDA were increased significantly (p = 0.011, 0.037, 0.018, respectively). Following the crocetin treatment at 10 μM, SOD and GSH activities were improved compared to the no drug group; and MDA level was declined remarkably (p = 0.022, 0.019, 0.029, respectively). The Bcl-2 level was significantly decreased (p |
| doi_str_mv | 10.1016/j.exer.2022.109305 |
| format | Article |
| fullrecord | <record><control><sourceid>pubmed</sourceid><recordid>TN_cdi_pubmed_primary_36372214</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>36372214</sourcerecordid><originalsourceid>FETCH-pubmed_primary_363722143</originalsourceid><addsrcrecordid>eNqFjcsKgkAYhYcgyi4v0CL-F9DmYorLCMNFUIR70fGXJixlZgx9-1zUOjhwPj4OHEI2jHqMsmD38LBH7XHK-SgiQfcT4owQuJTScE4WxjxGEn7oz8hcBCLknPkOOad3BKwqlNZAU4HUjUSrXtCM6VWZW_VGMFajMaBeZSexhMPtGrssAol1baAYIAEOF-ArMq3y2uD620uyPcXpMXHbrnhimbVaPXM9ZL978XfwAdinPmg</addsrcrecordid><sourcetype>Index Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>The effects of crocetin on oxidative stress induced ARPE-19 cells by H 2 O 2</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Demirci Kucuk, Kübra ; Tokuc, Ecem Onder ; Aciksari, Aysegul ; Duruksu, Gokhan ; Yazir, Yusufhan ; Karabas, Veysel Levent</creator><creatorcontrib>Demirci Kucuk, Kübra ; Tokuc, Ecem Onder ; Aciksari, Aysegul ; Duruksu, Gokhan ; Yazir, Yusufhan ; Karabas, Veysel Levent</creatorcontrib><description>The anti-apoptotic and antioxidant effects of crocetin was aimed to investigate on the oxidative damage model of ARPE-19 cells. The oxidative damage in ARPE cells was developed by H
O
treatment at 800 μM. Different doses of crocetin (1-80 μM) were applied for 24 h, and the effects on viability were evaluated to find out the optimum drug dose. At first, three effective doses of crocetin (10, 20, 40 μM) on cell viability were selected for further analyses. The levels of superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione (GSH) were determined, and the expression of pro-apoptotic Bax gene and anti-apoptotic Bcl-2 gene were evaluated. The most effective crocetin dose on cell viability was found to be 10 μM. After the H
O
treatment, SOD and GSH were decreased and MDA were increased significantly (p = 0.011, 0.037, 0.018, respectively). Following the crocetin treatment at 10 μM, SOD and GSH activities were improved compared to the no drug group; and MDA level was declined remarkably (p = 0.022, 0.019, 0.029, respectively). The Bcl-2 level was significantly decreased (p < 0.01), while the Bax1 and Nrf2 expression and ROS level was increased significantly in the damage model group (p < 0.01). After the drug treatment, the Bax1 and Nrf2 expression level were decreased in all groups (p < 0.01). The increase in Bcl-2 expression was significant in crocetin 40 μM (p < 0.05) and the decrease in ROS level were significant in 20 μM and 40 μM doses of crocetin (p < 0.05). It has been shown that crocetin might be used as an antioxidant and anti-apoptotic agent on the hindering the effect of the oxidative damage. Following the development of the oxidative stress in the cells, crocetin reversed the damage signals. By the in vitro tests, it was shown that crocetin might be considered as an effective molecule to be used in the AMD treatment.</description><identifier>EISSN: 1096-0007</identifier><identifier>DOI: 10.1016/j.exer.2022.109305</identifier><identifier>PMID: 36372214</identifier><language>eng</language><publisher>England</publisher><subject>Antioxidants - metabolism ; Antioxidants - pharmacology ; Apoptosis ; Cell Line ; Cell Survival ; Glutathione - metabolism ; Hydrogen Peroxide - toxicity ; NF-E2-Related Factor 2 - metabolism ; Oxidative Stress ; Proto-Oncogene Proteins c-bcl-2 - genetics ; Proto-Oncogene Proteins c-bcl-2 - metabolism ; Reactive Oxygen Species - metabolism ; Superoxide Dismutase - metabolism</subject><ispartof>Experimental eye research, 2023-01, Vol.226, p.109305</ispartof><rights>Copyright © 2022 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36372214$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Demirci Kucuk, Kübra</creatorcontrib><creatorcontrib>Tokuc, Ecem Onder</creatorcontrib><creatorcontrib>Aciksari, Aysegul</creatorcontrib><creatorcontrib>Duruksu, Gokhan</creatorcontrib><creatorcontrib>Yazir, Yusufhan</creatorcontrib><creatorcontrib>Karabas, Veysel Levent</creatorcontrib><title>The effects of crocetin on oxidative stress induced ARPE-19 cells by H 2 O 2</title><title>Experimental eye research</title><addtitle>Exp Eye Res</addtitle><description>The anti-apoptotic and antioxidant effects of crocetin was aimed to investigate on the oxidative damage model of ARPE-19 cells. The oxidative damage in ARPE cells was developed by H
O
treatment at 800 μM. Different doses of crocetin (1-80 μM) were applied for 24 h, and the effects on viability were evaluated to find out the optimum drug dose. At first, three effective doses of crocetin (10, 20, 40 μM) on cell viability were selected for further analyses. The levels of superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione (GSH) were determined, and the expression of pro-apoptotic Bax gene and anti-apoptotic Bcl-2 gene were evaluated. The most effective crocetin dose on cell viability was found to be 10 μM. After the H
O
treatment, SOD and GSH were decreased and MDA were increased significantly (p = 0.011, 0.037, 0.018, respectively). Following the crocetin treatment at 10 μM, SOD and GSH activities were improved compared to the no drug group; and MDA level was declined remarkably (p = 0.022, 0.019, 0.029, respectively). The Bcl-2 level was significantly decreased (p < 0.01), while the Bax1 and Nrf2 expression and ROS level was increased significantly in the damage model group (p < 0.01). After the drug treatment, the Bax1 and Nrf2 expression level were decreased in all groups (p < 0.01). The increase in Bcl-2 expression was significant in crocetin 40 μM (p < 0.05) and the decrease in ROS level were significant in 20 μM and 40 μM doses of crocetin (p < 0.05). It has been shown that crocetin might be used as an antioxidant and anti-apoptotic agent on the hindering the effect of the oxidative damage. Following the development of the oxidative stress in the cells, crocetin reversed the damage signals. By the in vitro tests, it was shown that crocetin might be considered as an effective molecule to be used in the AMD treatment.</description><subject>Antioxidants - metabolism</subject><subject>Antioxidants - pharmacology</subject><subject>Apoptosis</subject><subject>Cell Line</subject><subject>Cell Survival</subject><subject>Glutathione - metabolism</subject><subject>Hydrogen Peroxide - toxicity</subject><subject>NF-E2-Related Factor 2 - metabolism</subject><subject>Oxidative Stress</subject><subject>Proto-Oncogene Proteins c-bcl-2 - genetics</subject><subject>Proto-Oncogene Proteins c-bcl-2 - metabolism</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Superoxide Dismutase - metabolism</subject><issn>1096-0007</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFjcsKgkAYhYcgyi4v0CL-F9DmYorLCMNFUIR70fGXJixlZgx9-1zUOjhwPj4OHEI2jHqMsmD38LBH7XHK-SgiQfcT4owQuJTScE4WxjxGEn7oz8hcBCLknPkOOad3BKwqlNZAU4HUjUSrXtCM6VWZW_VGMFajMaBeZSexhMPtGrssAol1baAYIAEOF-ArMq3y2uD620uyPcXpMXHbrnhimbVaPXM9ZL978XfwAdinPmg</recordid><startdate>202301</startdate><enddate>202301</enddate><creator>Demirci Kucuk, Kübra</creator><creator>Tokuc, Ecem Onder</creator><creator>Aciksari, Aysegul</creator><creator>Duruksu, Gokhan</creator><creator>Yazir, Yusufhan</creator><creator>Karabas, Veysel Levent</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>202301</creationdate><title>The effects of crocetin on oxidative stress induced ARPE-19 cells by H 2 O 2</title><author>Demirci Kucuk, Kübra ; Tokuc, Ecem Onder ; Aciksari, Aysegul ; Duruksu, Gokhan ; Yazir, Yusufhan ; Karabas, Veysel Levent</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-pubmed_primary_363722143</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Antioxidants - metabolism</topic><topic>Antioxidants - pharmacology</topic><topic>Apoptosis</topic><topic>Cell Line</topic><topic>Cell Survival</topic><topic>Glutathione - metabolism</topic><topic>Hydrogen Peroxide - toxicity</topic><topic>NF-E2-Related Factor 2 - metabolism</topic><topic>Oxidative Stress</topic><topic>Proto-Oncogene Proteins c-bcl-2 - genetics</topic><topic>Proto-Oncogene Proteins c-bcl-2 - metabolism</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Superoxide Dismutase - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Demirci Kucuk, Kübra</creatorcontrib><creatorcontrib>Tokuc, Ecem Onder</creatorcontrib><creatorcontrib>Aciksari, Aysegul</creatorcontrib><creatorcontrib>Duruksu, Gokhan</creatorcontrib><creatorcontrib>Yazir, Yusufhan</creatorcontrib><creatorcontrib>Karabas, Veysel Levent</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>Experimental eye research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Demirci Kucuk, Kübra</au><au>Tokuc, Ecem Onder</au><au>Aciksari, Aysegul</au><au>Duruksu, Gokhan</au><au>Yazir, Yusufhan</au><au>Karabas, Veysel Levent</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The effects of crocetin on oxidative stress induced ARPE-19 cells by H 2 O 2</atitle><jtitle>Experimental eye research</jtitle><addtitle>Exp Eye Res</addtitle><date>2023-01</date><risdate>2023</risdate><volume>226</volume><spage>109305</spage><pages>109305-</pages><eissn>1096-0007</eissn><abstract>The anti-apoptotic and antioxidant effects of crocetin was aimed to investigate on the oxidative damage model of ARPE-19 cells. The oxidative damage in ARPE cells was developed by H
O
treatment at 800 μM. Different doses of crocetin (1-80 μM) were applied for 24 h, and the effects on viability were evaluated to find out the optimum drug dose. At first, three effective doses of crocetin (10, 20, 40 μM) on cell viability were selected for further analyses. The levels of superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione (GSH) were determined, and the expression of pro-apoptotic Bax gene and anti-apoptotic Bcl-2 gene were evaluated. The most effective crocetin dose on cell viability was found to be 10 μM. After the H
O
treatment, SOD and GSH were decreased and MDA were increased significantly (p = 0.011, 0.037, 0.018, respectively). Following the crocetin treatment at 10 μM, SOD and GSH activities were improved compared to the no drug group; and MDA level was declined remarkably (p = 0.022, 0.019, 0.029, respectively). The Bcl-2 level was significantly decreased (p < 0.01), while the Bax1 and Nrf2 expression and ROS level was increased significantly in the damage model group (p < 0.01). After the drug treatment, the Bax1 and Nrf2 expression level were decreased in all groups (p < 0.01). The increase in Bcl-2 expression was significant in crocetin 40 μM (p < 0.05) and the decrease in ROS level were significant in 20 μM and 40 μM doses of crocetin (p < 0.05). It has been shown that crocetin might be used as an antioxidant and anti-apoptotic agent on the hindering the effect of the oxidative damage. Following the development of the oxidative stress in the cells, crocetin reversed the damage signals. By the in vitro tests, it was shown that crocetin might be considered as an effective molecule to be used in the AMD treatment.</abstract><cop>England</cop><pmid>36372214</pmid><doi>10.1016/j.exer.2022.109305</doi></addata></record> |
| fulltext | fulltext |
| identifier | EISSN: 1096-0007 |
| ispartof | Experimental eye research, 2023-01, Vol.226, p.109305 |
| issn | 1096-0007 |
| language | eng |
| recordid | cdi_pubmed_primary_36372214 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Antioxidants - metabolism Antioxidants - pharmacology Apoptosis Cell Line Cell Survival Glutathione - metabolism Hydrogen Peroxide - toxicity NF-E2-Related Factor 2 - metabolism Oxidative Stress Proto-Oncogene Proteins c-bcl-2 - genetics Proto-Oncogene Proteins c-bcl-2 - metabolism Reactive Oxygen Species - metabolism Superoxide Dismutase - metabolism |
| title | The effects of crocetin on oxidative stress induced ARPE-19 cells by H 2 O 2 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-26T11%3A32%3A56IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-pubmed&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20effects%20of%20crocetin%20on%20oxidative%20stress%20induced%20ARPE-19%20cells%20by%20H%202%20O%202&rft.jtitle=Experimental%20eye%20research&rft.au=Demirci%20Kucuk,%20K%C3%BCbra&rft.date=2023-01&rft.volume=226&rft.spage=109305&rft.pages=109305-&rft.eissn=1096-0007&rft_id=info:doi/10.1016/j.exer.2022.109305&rft_dat=%3Cpubmed%3E36372214%3C/pubmed%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/36372214&rfr_iscdi=true |