Clinical training and validation of the LeukoScope: a low-cost, point-of-care device to perform white blood cell and neutrophil counts
A white blood cell (WBC) count with partial differential is an important clinical laboratory test. However, current methods to perform a WBC count and differential are difficult to use at the point of care or too expensive for use in low-resource settings. To meet this need, we developed the LeukoSc...
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| Veröffentlicht in: | RSC advances 2019-08, Vol.9 (47), p.27324-27333 |
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| creator | Majors, Catherine E Pawlowski, Michal E Burke, Daniel C Tkaczyk, Tomasz S Rieber, Alyssa Richards-Kortum, Rebecca |
| description | A white blood cell (WBC) count with partial differential is an important clinical laboratory test. However, current methods to perform a WBC count and differential are difficult to use at the point of care or too expensive for use in low-resource settings. To meet this need, we developed the LeukoScope: a low-cost system to measure a WBC and neutrophil count from a single drop of blood at the point of care. The LeukoScope is battery powered and has a sample-to-answer time of |
| doi_str_mv | 10.1039/c9ra06433h |
| format | Article |
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We present a novel, point-of-care method to perform WBC and neutrophil counts with a drop of blood and portable reader.</description><subject>Blood</subject><subject>Cartridges</subject><subject>Chemistry</subject><subject>Color imagery</subject><subject>Fluorescence</subject><subject>Laboratories</subject><subject>Laboratory tests</subject><subject>Leukocytes</subject><subject>Low cost</subject><subject>Neutrophils</subject><issn>2046-2069</issn><issn>2046-2069</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNpdkk1v1DAQhiMEolXphTvIEheECPgjcRIOSNWqpUgrIfFxthxn0rh4PcF2tuIP8LvxdstS8MVjzaN35tXronjK6BtGRffWdEFTWQkxPSiOOa1kyansHt6rj4rTGK9pPrJmXLLHxZGoa97xih8Xv1bOemu0IynoXPkrov1AttrZQSeLnuBI0gRkDct3_GJwhndEE4c3pcGYXpMZrU8ljqXRAcgAW2uAJCQzhBHDhtxMNgHpHeJADDh3K-9hSQHnyTpicPEpPikejdpFOL27T4pvF-dfV5fl-tOHj6uzdWkq3qZyEL0WI-MtNz0Izfq2o7URQldNS_u2qfKL0xGahumhEUZAVTdyFIPW2XsjxUnxfq87L_0GBgM-23ZqDnajw0-F2qp_O95O6gq3qqMNlbzKAi_vBAL-WCAmtbFx50t7wCUqLiWrWtmy3awX_6HXuASf7SnOW5GjqZs2U6_2lAkYY4DxsAyjapewWnWfz24Tvszw8_vrH9A_eWbg2R4I0Ry6f7-I-A1r_axQ</recordid><startdate>20190830</startdate><enddate>20190830</enddate><creator>Majors, Catherine E</creator><creator>Pawlowski, Michal E</creator><creator>Burke, Daniel C</creator><creator>Tkaczyk, Tomasz S</creator><creator>Rieber, Alyssa</creator><creator>Richards-Kortum, Rebecca</creator><general>Royal Society of Chemistry</general><general>The Royal Society of Chemistry</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-6004-2507</orcidid><orcidid>https://orcid.org/0000-0001-7153-3312</orcidid><orcidid>https://orcid.org/0000-0002-5741-5819</orcidid><orcidid>https://orcid.org/0000-0003-2347-9467</orcidid></search><sort><creationdate>20190830</creationdate><title>Clinical training and validation of the LeukoScope: a low-cost, point-of-care device to perform white blood cell and neutrophil counts</title><author>Majors, Catherine E ; Pawlowski, Michal E ; Burke, Daniel C ; Tkaczyk, Tomasz S ; Rieber, Alyssa ; Richards-Kortum, Rebecca</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c428t-d3ba3f1282cbe3a1b8905c33a4780b87405c20fe771ad73c3e4576f3daa651763</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Blood</topic><topic>Cartridges</topic><topic>Chemistry</topic><topic>Color imagery</topic><topic>Fluorescence</topic><topic>Laboratories</topic><topic>Laboratory tests</topic><topic>Leukocytes</topic><topic>Low cost</topic><topic>Neutrophils</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Majors, Catherine E</creatorcontrib><creatorcontrib>Pawlowski, Michal E</creatorcontrib><creatorcontrib>Burke, Daniel C</creatorcontrib><creatorcontrib>Tkaczyk, Tomasz S</creatorcontrib><creatorcontrib>Rieber, Alyssa</creatorcontrib><creatorcontrib>Richards-Kortum, Rebecca</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>RSC advances</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Majors, Catherine E</au><au>Pawlowski, Michal E</au><au>Burke, Daniel C</au><au>Tkaczyk, Tomasz S</au><au>Rieber, Alyssa</au><au>Richards-Kortum, Rebecca</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Clinical training and validation of the LeukoScope: a low-cost, point-of-care device to perform white blood cell and neutrophil counts</atitle><jtitle>RSC advances</jtitle><addtitle>RSC Adv</addtitle><date>2019-08-30</date><risdate>2019</risdate><volume>9</volume><issue>47</issue><spage>27324</spage><epage>27333</epage><pages>27324-27333</pages><issn>2046-2069</issn><eissn>2046-2069</eissn><abstract>A white blood cell (WBC) count with partial differential is an important clinical laboratory test. However, current methods to perform a WBC count and differential are difficult to use at the point of care or too expensive for use in low-resource settings. To meet this need, we developed the LeukoScope: a low-cost system to measure a WBC and neutrophil count from a single drop of blood at the point of care. The LeukoScope is battery powered and has a sample-to-answer time of <5 minutes. A drop of blood from a finger stick is added to a LeukoScope sample cartridge where pre-dried acridine orange fluorescently stains WBCs. The cartridge is then inserted into the LeukoScope reader where a portable fluorescence microscope captures a color image of the sample, which is analyzed to report results to the user. The LeukoScope system was tested at the point of care using fingerprick samples collected from 105 general oncology patients in Houston, TX. Performance of the LeukoScope was compared to that of a HemoCue WBC DIFF performed using the same fingerprick sample; clinical laboratory analysis of a venous blood draw was used as the gold standard in all cases. BlandAltman analysis showed that the LeukoScope and HemoCue WBC DIFF had similar accuracy for measurement of WBC and neutrophil counts as compared to the gold standard. Seven out of eight patients with abnormal WBC count values were correctly identified using the LeukoScope, while six out of eight were correctly identified using the HemoCue WBC DIFF. Five out of six patients with abnormal neutrophil counts were correctly identified using the LeukoScope, while six of six were correctly identified using the HemoCue WBC DIFF.
We present a novel, point-of-care method to perform WBC and neutrophil counts with a drop of blood and portable reader.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>35529242</pmid><doi>10.1039/c9ra06433h</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-6004-2507</orcidid><orcidid>https://orcid.org/0000-0001-7153-3312</orcidid><orcidid>https://orcid.org/0000-0002-5741-5819</orcidid><orcidid>https://orcid.org/0000-0003-2347-9467</orcidid><oa>free_for_read</oa></addata></record> |
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| source | DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; PubMed Central |
| subjects | Blood Cartridges Chemistry Color imagery Fluorescence Laboratories Laboratory tests Leukocytes Low cost Neutrophils |
| title | Clinical training and validation of the LeukoScope: a low-cost, point-of-care device to perform white blood cell and neutrophil counts |
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