Bifunctional nickel-iminodiacetic acid-core-shell silica nanoparticles for the exclusion of high molecular weight proteins and purification of His-tagged recombinant proteins

Herein, silica nanoparticles were synthesized and chemically modified with iminodiacetic acid (IDA) and Ni 2+ ions surrounded by a bis-acrylamide polymeric shell to obtain a new core-shell immobilized metal affinity chromatography (IMAC) based material. These Ni 2+ -IDA-core-shell silica nanoparticl...

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Veröffentlicht in:	RSC advances 2019-04, Vol.9 (2), p.1138-1145
Hauptverfasser:	Hernandez-Leon, Sergio G, Sarabia-Sainz, Jose A, Ramos-Clamont Montfort, Gabriela, Huerta-Ocampo, José Ángel, Guzman-Partida, Ana M, Robles-Burgueño, Maria del Refugio, Burgara-Estrella, Alexel J, Vazquez-Moreno, Luz
Format:	Artikel
Sprache:	eng
Schlagworte:	Acrylamide Chemical synthesis Chemistry E coli Mass spectrometry Molecular weight Nanoparticles Nickel Organic chemistry Photon correlation spectroscopy Proteins Purification Selectivity Silicon dioxide Surface charge
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