The suppression effect of SCH-79797 on Streptococcus mutans biofilm formation
SCH-79797 was recently shown to be a broad-spectrum antibacterial agent with a dual-bactericidal mechanism. However, its anti-biofilm effect remains unknown. To investigate the effect of SCH-79797 on the biofilm formation of the cariogenic Streptococcus mutans Crystal violet staining, colony forming...
Gespeichert in:
| Veröffentlicht in: | Journal of oral microbiology 2022-12, Vol.14 (1), p.2061113-2061113 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 2061113 |
|---|---|
| container_issue | 1 |
| container_start_page | 2061113 |
| container_title | Journal of oral microbiology |
| container_volume | 14 |
| creator | Zhang, Lingjun Shen, Yan Qiu, Lili Yu, Fangzheng Hu, Xiangyu Wang, Min Sun, Yan Pan, Yihuai Zhang, Keke |
| description | SCH-79797 was recently shown to be a broad-spectrum antibacterial agent with a dual-bactericidal mechanism. However, its anti-biofilm effect remains unknown.
To investigate the effect of SCH-79797 on the biofilm formation of the cariogenic Streptococcus mutans
Crystal violet staining, colony forming units count and MTT assays (for cell metabolic activity) revealed that S. mutans biofilm formation was significantly suppressed. In addition, virulence factors, including extracellular polysaccharides (investigated by bacterial/exopolysaccharide staining and the anthrone method) and acid production (investigated by lactic acid and supernatant pH detection) were also inhibited significantly. Moreover, the biofilm inhibitory effect of SCH-79797 was mediated through its repression of bacterial growth and not by a bactericidal effect, which was verified by growth curve and bacterial live/ dead staining, respectively. Quantitative real-time PCR results disclosed that SCH-79797 affected bacterial acid production and tolerance, polysaccharide synthesis and remodeling, biofilm formation and quorum sensing-related gene expression. In addition, SCH-79797 showed good biocompatibility as determined by cytotoxicity assays.
SCH-79797 had an anti-biofilm effect and showed application prospects in the control of dental caries. |
| doi_str_mv | 10.1080/20002297.2022.2061113 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmed_primary_35480051</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_d20b61de3075416192b6318c3807e250</doaj_id><sourcerecordid>2656742097</sourcerecordid><originalsourceid>FETCH-LOGICAL-c534t-d7ab41a619deb9a99ec15ea806649191eb98d04797a3afa14b4b9463d2cf02073</originalsourceid><addsrcrecordid>eNp9UctO3TAUtFArQJRPaJVlNxeOH7HjTdUKlYdE1QV0bTl-gFESp7bTir-vw70g2FSWfOw5c2aONAh9xHCCoYNTAgCESHFCaqkXxxjTPXS44pu18e7V-wAd5_xQf0BJ1zGyjw5oyzqAFh-iH7f3rsnLPCeXc4hT47x3pjTRNzdnlxsh62kqfFOSm0s00ZglN-NS9JSbPkQfhrHxMY261OkP6L3XQ3bHu3qEfp1_v6061z8vrs6-XW9MS1nZWKF7hjXH0rpeaimdwa3THXDOJJa4gp0FVq011V5j1rNeMk4tMR4ICHqErra6NuoHNacw6vSoog7qCYjpTulUghmcsgR6jq2jIFqGqyXpOcWdoR0IR1qoWl-2WvPSj84aN5WkhzeibztTuFd38Y-SQAUWuAp83gmk-HtxuagxZOOGQU8uLlkR3nLBCMh173ZLNSnmnJx_scGg1mTVc7JqTVbtkq1zn17v-DL1nGMlfN0SwvQUxt-YBquKfhxi8klPJmRF_-_xD7h-sJw</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2656742097</pqid></control><display><type>article</type><title>The suppression effect of SCH-79797 on Streptococcus mutans biofilm formation</title><source>Taylor & Francis</source><source>DOAJ Directory of Open Access Journals</source><source>PubMed</source><source>Co-Action Open Access Journals</source><source>EZB Electronic Journals Library</source><creator>Zhang, Lingjun ; Shen, Yan ; Qiu, Lili ; Yu, Fangzheng ; Hu, Xiangyu ; Wang, Min ; Sun, Yan ; Pan, Yihuai ; Zhang, Keke</creator><creatorcontrib>Zhang, Lingjun ; Shen, Yan ; Qiu, Lili ; Yu, Fangzheng ; Hu, Xiangyu ; Wang, Min ; Sun, Yan ; Pan, Yihuai ; Zhang, Keke</creatorcontrib><description>SCH-79797 was recently shown to be a broad-spectrum antibacterial agent with a dual-bactericidal mechanism. However, its anti-biofilm effect remains unknown.
To investigate the effect of SCH-79797 on the biofilm formation of the cariogenic Streptococcus mutans
Crystal violet staining, colony forming units count and MTT assays (for cell metabolic activity) revealed that S. mutans biofilm formation was significantly suppressed. In addition, virulence factors, including extracellular polysaccharides (investigated by bacterial/exopolysaccharide staining and the anthrone method) and acid production (investigated by lactic acid and supernatant pH detection) were also inhibited significantly. Moreover, the biofilm inhibitory effect of SCH-79797 was mediated through its repression of bacterial growth and not by a bactericidal effect, which was verified by growth curve and bacterial live/ dead staining, respectively. Quantitative real-time PCR results disclosed that SCH-79797 affected bacterial acid production and tolerance, polysaccharide synthesis and remodeling, biofilm formation and quorum sensing-related gene expression. In addition, SCH-79797 showed good biocompatibility as determined by cytotoxicity assays.
SCH-79797 had an anti-biofilm effect and showed application prospects in the control of dental caries.</description><identifier>ISSN: 2000-2297</identifier><identifier>EISSN: 2000-2297</identifier><identifier>DOI: 10.1080/20002297.2022.2061113</identifier><identifier>PMID: 35480051</identifier><language>eng</language><publisher>United States: Taylor & Francis</publisher><subject>biofilms ; cariogenic virulence ; dental caries ; Original ; SCH-79797 ; Streptococcus mutans</subject><ispartof>Journal of oral microbiology, 2022-12, Vol.14 (1), p.2061113-2061113</ispartof><rights>2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. 2022</rights><rights>2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.</rights><rights>2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. 2022 The Author(s)</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c534t-d7ab41a619deb9a99ec15ea806649191eb98d04797a3afa14b4b9463d2cf02073</citedby><cites>FETCH-LOGICAL-c534t-d7ab41a619deb9a99ec15ea806649191eb98d04797a3afa14b4b9463d2cf02073</cites><orcidid>0000-0002-5755-7059</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9037171/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9037171/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,861,882,2096,27483,27905,27906,53772,53774,59122,59123</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35480051$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Lingjun</creatorcontrib><creatorcontrib>Shen, Yan</creatorcontrib><creatorcontrib>Qiu, Lili</creatorcontrib><creatorcontrib>Yu, Fangzheng</creatorcontrib><creatorcontrib>Hu, Xiangyu</creatorcontrib><creatorcontrib>Wang, Min</creatorcontrib><creatorcontrib>Sun, Yan</creatorcontrib><creatorcontrib>Pan, Yihuai</creatorcontrib><creatorcontrib>Zhang, Keke</creatorcontrib><title>The suppression effect of SCH-79797 on Streptococcus mutans biofilm formation</title><title>Journal of oral microbiology</title><addtitle>J Oral Microbiol</addtitle><description>SCH-79797 was recently shown to be a broad-spectrum antibacterial agent with a dual-bactericidal mechanism. However, its anti-biofilm effect remains unknown.
To investigate the effect of SCH-79797 on the biofilm formation of the cariogenic Streptococcus mutans
Crystal violet staining, colony forming units count and MTT assays (for cell metabolic activity) revealed that S. mutans biofilm formation was significantly suppressed. In addition, virulence factors, including extracellular polysaccharides (investigated by bacterial/exopolysaccharide staining and the anthrone method) and acid production (investigated by lactic acid and supernatant pH detection) were also inhibited significantly. Moreover, the biofilm inhibitory effect of SCH-79797 was mediated through its repression of bacterial growth and not by a bactericidal effect, which was verified by growth curve and bacterial live/ dead staining, respectively. Quantitative real-time PCR results disclosed that SCH-79797 affected bacterial acid production and tolerance, polysaccharide synthesis and remodeling, biofilm formation and quorum sensing-related gene expression. In addition, SCH-79797 showed good biocompatibility as determined by cytotoxicity assays.
SCH-79797 had an anti-biofilm effect and showed application prospects in the control of dental caries.</description><subject>biofilms</subject><subject>cariogenic virulence</subject><subject>dental caries</subject><subject>Original</subject><subject>SCH-79797</subject><subject>Streptococcus mutans</subject><issn>2000-2297</issn><issn>2000-2297</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>0YH</sourceid><sourceid>DOA</sourceid><recordid>eNp9UctO3TAUtFArQJRPaJVlNxeOH7HjTdUKlYdE1QV0bTl-gFESp7bTir-vw70g2FSWfOw5c2aONAh9xHCCoYNTAgCESHFCaqkXxxjTPXS44pu18e7V-wAd5_xQf0BJ1zGyjw5oyzqAFh-iH7f3rsnLPCeXc4hT47x3pjTRNzdnlxsh62kqfFOSm0s00ZglN-NS9JSbPkQfhrHxMY261OkP6L3XQ3bHu3qEfp1_v6061z8vrs6-XW9MS1nZWKF7hjXH0rpeaimdwa3THXDOJJa4gp0FVq011V5j1rNeMk4tMR4ICHqErra6NuoHNacw6vSoog7qCYjpTulUghmcsgR6jq2jIFqGqyXpOcWdoR0IR1qoWl-2WvPSj84aN5WkhzeibztTuFd38Y-SQAUWuAp83gmk-HtxuagxZOOGQU8uLlkR3nLBCMh173ZLNSnmnJx_scGg1mTVc7JqTVbtkq1zn17v-DL1nGMlfN0SwvQUxt-YBquKfhxi8klPJmRF_-_xD7h-sJw</recordid><startdate>20221231</startdate><enddate>20221231</enddate><creator>Zhang, Lingjun</creator><creator>Shen, Yan</creator><creator>Qiu, Lili</creator><creator>Yu, Fangzheng</creator><creator>Hu, Xiangyu</creator><creator>Wang, Min</creator><creator>Sun, Yan</creator><creator>Pan, Yihuai</creator><creator>Zhang, Keke</creator><general>Taylor & Francis</general><general>Taylor & Francis Group</general><scope>0YH</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-5755-7059</orcidid></search><sort><creationdate>20221231</creationdate><title>The suppression effect of SCH-79797 on Streptococcus mutans biofilm formation</title><author>Zhang, Lingjun ; Shen, Yan ; Qiu, Lili ; Yu, Fangzheng ; Hu, Xiangyu ; Wang, Min ; Sun, Yan ; Pan, Yihuai ; Zhang, Keke</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c534t-d7ab41a619deb9a99ec15ea806649191eb98d04797a3afa14b4b9463d2cf02073</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>biofilms</topic><topic>cariogenic virulence</topic><topic>dental caries</topic><topic>Original</topic><topic>SCH-79797</topic><topic>Streptococcus mutans</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Lingjun</creatorcontrib><creatorcontrib>Shen, Yan</creatorcontrib><creatorcontrib>Qiu, Lili</creatorcontrib><creatorcontrib>Yu, Fangzheng</creatorcontrib><creatorcontrib>Hu, Xiangyu</creatorcontrib><creatorcontrib>Wang, Min</creatorcontrib><creatorcontrib>Sun, Yan</creatorcontrib><creatorcontrib>Pan, Yihuai</creatorcontrib><creatorcontrib>Zhang, Keke</creatorcontrib><collection>Taylor & Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Journal of oral microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Lingjun</au><au>Shen, Yan</au><au>Qiu, Lili</au><au>Yu, Fangzheng</au><au>Hu, Xiangyu</au><au>Wang, Min</au><au>Sun, Yan</au><au>Pan, Yihuai</au><au>Zhang, Keke</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The suppression effect of SCH-79797 on Streptococcus mutans biofilm formation</atitle><jtitle>Journal of oral microbiology</jtitle><addtitle>J Oral Microbiol</addtitle><date>2022-12-31</date><risdate>2022</risdate><volume>14</volume><issue>1</issue><spage>2061113</spage><epage>2061113</epage><pages>2061113-2061113</pages><issn>2000-2297</issn><eissn>2000-2297</eissn><abstract>SCH-79797 was recently shown to be a broad-spectrum antibacterial agent with a dual-bactericidal mechanism. However, its anti-biofilm effect remains unknown.
To investigate the effect of SCH-79797 on the biofilm formation of the cariogenic Streptococcus mutans
Crystal violet staining, colony forming units count and MTT assays (for cell metabolic activity) revealed that S. mutans biofilm formation was significantly suppressed. In addition, virulence factors, including extracellular polysaccharides (investigated by bacterial/exopolysaccharide staining and the anthrone method) and acid production (investigated by lactic acid and supernatant pH detection) were also inhibited significantly. Moreover, the biofilm inhibitory effect of SCH-79797 was mediated through its repression of bacterial growth and not by a bactericidal effect, which was verified by growth curve and bacterial live/ dead staining, respectively. Quantitative real-time PCR results disclosed that SCH-79797 affected bacterial acid production and tolerance, polysaccharide synthesis and remodeling, biofilm formation and quorum sensing-related gene expression. In addition, SCH-79797 showed good biocompatibility as determined by cytotoxicity assays.
SCH-79797 had an anti-biofilm effect and showed application prospects in the control of dental caries.</abstract><cop>United States</cop><pub>Taylor & Francis</pub><pmid>35480051</pmid><doi>10.1080/20002297.2022.2061113</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-5755-7059</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2000-2297 |
| ispartof | Journal of oral microbiology, 2022-12, Vol.14 (1), p.2061113-2061113 |
| issn | 2000-2297 2000-2297 |
| language | eng |
| recordid | cdi_pubmed_primary_35480051 |
| source | Taylor & Francis; DOAJ Directory of Open Access Journals; PubMed; Co-Action Open Access Journals; EZB Electronic Journals Library |
| subjects | biofilms cariogenic virulence dental caries Original SCH-79797 Streptococcus mutans |
| title | The suppression effect of SCH-79797 on Streptococcus mutans biofilm formation |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-17T18%3A22%3A56IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20suppression%20effect%20of%20SCH-79797%20on%20Streptococcus%20mutans%20biofilm%20formation&rft.jtitle=Journal%20of%20oral%20microbiology&rft.au=Zhang,%20Lingjun&rft.date=2022-12-31&rft.volume=14&rft.issue=1&rft.spage=2061113&rft.epage=2061113&rft.pages=2061113-2061113&rft.issn=2000-2297&rft.eissn=2000-2297&rft_id=info:doi/10.1080/20002297.2022.2061113&rft_dat=%3Cproquest_pubme%3E2656742097%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2656742097&rft_id=info:pmid/35480051&rft_doaj_id=oai_doaj_org_article_d20b61de3075416192b6318c3807e250&rfr_iscdi=true |