Corepressors SsnF and RcoA Regulate Development and Aflatoxin B 1 Biosynthesis in Aspergillus flavus NRRL 3357
is a saprophytic fungus that can be found across the entire world. It can produce aflatoxin B (AFB ), which threatens human health. CreA, as the central factor in carbon catabolite repression (CCR), regulates carbon catabolism and AFB biosynthesis in . Additionally, SsnF-RcoA are recognized as the c...
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| Veröffentlicht in: | Toxins 2022-02, Vol.14 (3) |
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| creator | Ma, Xiaoyun Jiang, Yiran Ma, Longxue Luo, Shujuan Du, Haolan Li, Xu Xing, Fuguo |
| description | is a saprophytic fungus that can be found across the entire world. It can produce aflatoxin B
(AFB
), which threatens human health. CreA, as the central factor in carbon catabolite repression (CCR), regulates carbon catabolism and AFB
biosynthesis in
. Additionally, SsnF-RcoA are recognized as the corepressors of CreA in CCR. In this study,
and
not only regulated the expressions of CCR factors and hydrolase genes, but also positively affected mycelia growth, conidia production, sclerotia formation, and osmotic stress response in
. More importantly, SsnF and RcoA were identified as positive regulators for AFB
biosynthesis, as they modulate the AF cluster genes and the relevant regulators at a transcriptional level. Additionally, the interactions of SsnF-CreA and RcoA-CreA were strong and moderate, respectively. However, the interaction of SsnF and RcoA was weak. The interaction models of CreA-SsnF, CreA-RcoA, and SsnF-RcoA were also simulated with a docking analysis. All things considered, SsnF and RcoA are not just the critical regulators of the CCR pathway, but the global regulators involving in morphological development and AFB
biosynthesis in
. |
| doi_str_mv | 10.3390/toxins14030174 |
| format | Article |
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(AFB
), which threatens human health. CreA, as the central factor in carbon catabolite repression (CCR), regulates carbon catabolism and AFB
biosynthesis in
. Additionally, SsnF-RcoA are recognized as the corepressors of CreA in CCR. In this study,
and
not only regulated the expressions of CCR factors and hydrolase genes, but also positively affected mycelia growth, conidia production, sclerotia formation, and osmotic stress response in
. More importantly, SsnF and RcoA were identified as positive regulators for AFB
biosynthesis, as they modulate the AF cluster genes and the relevant regulators at a transcriptional level. Additionally, the interactions of SsnF-CreA and RcoA-CreA were strong and moderate, respectively. However, the interaction of SsnF and RcoA was weak. The interaction models of CreA-SsnF, CreA-RcoA, and SsnF-RcoA were also simulated with a docking analysis. All things considered, SsnF and RcoA are not just the critical regulators of the CCR pathway, but the global regulators involving in morphological development and AFB
biosynthesis in
.</description><identifier>EISSN: 2072-6651</identifier><identifier>DOI: 10.3390/toxins14030174</identifier><identifier>PMID: 35324671</identifier><language>eng</language><publisher>Switzerland</publisher><subject>Aflatoxin B1 - biosynthesis ; Aspergillus flavus - metabolism ; Co-Repressor Proteins - metabolism ; Fungal Proteins - genetics ; Fungal Proteins - metabolism ; Spores, Fungal</subject><ispartof>Toxins, 2022-02, Vol.14 (3)</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><orcidid>0000-0002-9078-6523</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,864,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35324671$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ma, Xiaoyun</creatorcontrib><creatorcontrib>Jiang, Yiran</creatorcontrib><creatorcontrib>Ma, Longxue</creatorcontrib><creatorcontrib>Luo, Shujuan</creatorcontrib><creatorcontrib>Du, Haolan</creatorcontrib><creatorcontrib>Li, Xu</creatorcontrib><creatorcontrib>Xing, Fuguo</creatorcontrib><title>Corepressors SsnF and RcoA Regulate Development and Aflatoxin B 1 Biosynthesis in Aspergillus flavus NRRL 3357</title><title>Toxins</title><addtitle>Toxins (Basel)</addtitle><description>is a saprophytic fungus that can be found across the entire world. It can produce aflatoxin B
(AFB
), which threatens human health. CreA, as the central factor in carbon catabolite repression (CCR), regulates carbon catabolism and AFB
biosynthesis in
. Additionally, SsnF-RcoA are recognized as the corepressors of CreA in CCR. In this study,
and
not only regulated the expressions of CCR factors and hydrolase genes, but also positively affected mycelia growth, conidia production, sclerotia formation, and osmotic stress response in
. More importantly, SsnF and RcoA were identified as positive regulators for AFB
biosynthesis, as they modulate the AF cluster genes and the relevant regulators at a transcriptional level. Additionally, the interactions of SsnF-CreA and RcoA-CreA were strong and moderate, respectively. However, the interaction of SsnF and RcoA was weak. The interaction models of CreA-SsnF, CreA-RcoA, and SsnF-RcoA were also simulated with a docking analysis. All things considered, SsnF and RcoA are not just the critical regulators of the CCR pathway, but the global regulators involving in morphological development and AFB
biosynthesis in
.</description><subject>Aflatoxin B1 - biosynthesis</subject><subject>Aspergillus flavus - metabolism</subject><subject>Co-Repressor Proteins - metabolism</subject><subject>Fungal Proteins - genetics</subject><subject>Fungal Proteins - metabolism</subject><subject>Spores, Fungal</subject><issn>2072-6651</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFjs1uwjAQhC2kqqCWa4_VvgDFziaxOIY_9YA4pL2jtCzUlWNb3gTB29etypm5fNLMSDNCPCn5gjiT086fjWOVS5RK5wMxyqTOJmVZqKEYM3_LJEQ1U_peDLHALC-1Ggm38JFCJGYfGd7YraFxe6g_fQU1HXvbdARLOpH1oSXX_aXVIdm_ezAHBXPj-eK6L2LDkLyKA8WjsbZnSMVTwrauN4BY6Edxd2gs0_ifD-J5vXpfvE5C_9HSfheiaZt42V0P4s3CD6pbTBo</recordid><startdate>20220225</startdate><enddate>20220225</enddate><creator>Ma, Xiaoyun</creator><creator>Jiang, Yiran</creator><creator>Ma, Longxue</creator><creator>Luo, Shujuan</creator><creator>Du, Haolan</creator><creator>Li, Xu</creator><creator>Xing, Fuguo</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><orcidid>https://orcid.org/0000-0002-9078-6523</orcidid></search><sort><creationdate>20220225</creationdate><title>Corepressors SsnF and RcoA Regulate Development and Aflatoxin B 1 Biosynthesis in Aspergillus flavus NRRL 3357</title><author>Ma, Xiaoyun ; Jiang, Yiran ; Ma, Longxue ; Luo, Shujuan ; Du, Haolan ; Li, Xu ; Xing, Fuguo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-pubmed_primary_353246713</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Aflatoxin B1 - biosynthesis</topic><topic>Aspergillus flavus - metabolism</topic><topic>Co-Repressor Proteins - metabolism</topic><topic>Fungal Proteins - genetics</topic><topic>Fungal Proteins - metabolism</topic><topic>Spores, Fungal</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ma, Xiaoyun</creatorcontrib><creatorcontrib>Jiang, Yiran</creatorcontrib><creatorcontrib>Ma, Longxue</creatorcontrib><creatorcontrib>Luo, Shujuan</creatorcontrib><creatorcontrib>Du, Haolan</creatorcontrib><creatorcontrib>Li, Xu</creatorcontrib><creatorcontrib>Xing, Fuguo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>Toxins</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ma, Xiaoyun</au><au>Jiang, Yiran</au><au>Ma, Longxue</au><au>Luo, Shujuan</au><au>Du, Haolan</au><au>Li, Xu</au><au>Xing, Fuguo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Corepressors SsnF and RcoA Regulate Development and Aflatoxin B 1 Biosynthesis in Aspergillus flavus NRRL 3357</atitle><jtitle>Toxins</jtitle><addtitle>Toxins (Basel)</addtitle><date>2022-02-25</date><risdate>2022</risdate><volume>14</volume><issue>3</issue><eissn>2072-6651</eissn><abstract>is a saprophytic fungus that can be found across the entire world. It can produce aflatoxin B
(AFB
), which threatens human health. CreA, as the central factor in carbon catabolite repression (CCR), regulates carbon catabolism and AFB
biosynthesis in
. Additionally, SsnF-RcoA are recognized as the corepressors of CreA in CCR. In this study,
and
not only regulated the expressions of CCR factors and hydrolase genes, but also positively affected mycelia growth, conidia production, sclerotia formation, and osmotic stress response in
. More importantly, SsnF and RcoA were identified as positive regulators for AFB
biosynthesis, as they modulate the AF cluster genes and the relevant regulators at a transcriptional level. Additionally, the interactions of SsnF-CreA and RcoA-CreA were strong and moderate, respectively. However, the interaction of SsnF and RcoA was weak. The interaction models of CreA-SsnF, CreA-RcoA, and SsnF-RcoA were also simulated with a docking analysis. All things considered, SsnF and RcoA are not just the critical regulators of the CCR pathway, but the global regulators involving in morphological development and AFB
biosynthesis in
.</abstract><cop>Switzerland</cop><pmid>35324671</pmid><doi>10.3390/toxins14030174</doi><orcidid>https://orcid.org/0000-0002-9078-6523</orcidid></addata></record> |
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| identifier | EISSN: 2072-6651 |
| ispartof | Toxins, 2022-02, Vol.14 (3) |
| issn | 2072-6651 |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; PubMed Central Open Access; MDPI - Multidisciplinary Digital Publishing Institute; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Aflatoxin B1 - biosynthesis Aspergillus flavus - metabolism Co-Repressor Proteins - metabolism Fungal Proteins - genetics Fungal Proteins - metabolism Spores, Fungal |
| title | Corepressors SsnF and RcoA Regulate Development and Aflatoxin B 1 Biosynthesis in Aspergillus flavus NRRL 3357 |
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