The evolving role of long noncoding RNA HIF1A-AS2 in diabetic retinopathy: a cross-link axis between hypoxia, oxidative stress and angiogenesis via MAPK/VEGF-dependent pathway
Diabetic retinopathy (DR) signifies a frequent serious diabetic complication influencing retinal structure and function. Dysregulation of lncRNAs drives a wide array of human diseases especially diabetes; thus, we aimed to study lncRNA HIF1A-AS2 role and its interplay with hypoxia, oxidative stress...
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| Veröffentlicht in: | Redox report : communications in free radical research 2022-12, Vol.27 (1), p.70-78 |
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| creator | Atef, Marwa Mohamed Shafik, Noha M. Hafez, Yasser Mostafa Watany, Mona Mohamed Selim, Amal Shafik, Heba M. Safwat El-Deeb, Omnia |
| description | Diabetic retinopathy (DR) signifies a frequent serious diabetic complication influencing retinal structure and function. Dysregulation of lncRNAs drives a wide array of human diseases especially diabetes; thus, we aimed to study lncRNA HIF1A-AS2 role and its interplay with hypoxia, oxidative stress (OS), and angiogenesis in DR.
60 DM patients in addition to 15 healthy subjects. were enrolled. LncRNA HIF1A-AS2 mRNA relative gene expression was assessed. Hypoxia inducible factor 1-alpha (HIF-1α), vascular endothelial growth factor (VEGF), mitogen activated protein kinase (MAPK), and endoglin levels were assessed. Detection of DNA damage using comet assay, and Redox status parameters were also detected.
LncRNA HIF1A-AS2 expression was significantly increased in diabetic patients with the highest levels in proliferative DR patients. Moreover, HIFα, VEGF, MAPK, and Endogolin levels were significantly higher in the diabetic patients compared to control group with the highest levels in in proliferative DR patients. Significant DNA damage in comet assay was observed to be the highest in this group.
We observed for the first time the imminent role of long noncoding RNA HIF1A-AS2 in DR throughout its stages and its interplay with hypoxia, OS, and angiogenesis via MAPK/VEGF-dependent pathway. |
| doi_str_mv | 10.1080/13510002.2022.2050086 |
| format | Article |
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60 DM patients in addition to 15 healthy subjects. were enrolled. LncRNA HIF1A-AS2 mRNA relative gene expression was assessed. Hypoxia inducible factor 1-alpha (HIF-1α), vascular endothelial growth factor (VEGF), mitogen activated protein kinase (MAPK), and endoglin levels were assessed. Detection of DNA damage using comet assay, and Redox status parameters were also detected.
LncRNA HIF1A-AS2 expression was significantly increased in diabetic patients with the highest levels in proliferative DR patients. Moreover, HIFα, VEGF, MAPK, and Endogolin levels were significantly higher in the diabetic patients compared to control group with the highest levels in in proliferative DR patients. Significant DNA damage in comet assay was observed to be the highest in this group.
We observed for the first time the imminent role of long noncoding RNA HIF1A-AS2 in DR throughout its stages and its interplay with hypoxia, OS, and angiogenesis via MAPK/VEGF-dependent pathway.</description><identifier>ISSN: 1351-0002</identifier><identifier>EISSN: 1743-2928</identifier><identifier>DOI: 10.1080/13510002.2022.2050086</identifier><identifier>PMID: 35285425</identifier><language>eng</language><publisher>England: Taylor & Francis</publisher><subject>angiogenesis ; Diabetes Mellitus ; Diabetic retinopathy ; Diabetic Retinopathy - genetics ; endoglin ; HIF-1α ; HIF1A-AS2 ; Humans ; Hypoxia - genetics ; Hypoxia-Inducible Factor 1, alpha Subunit - genetics ; Hypoxia-Inducible Factor 1, alpha Subunit - metabolism ; MAPK ; Mitogen-Activated Protein Kinases - metabolism ; oxidative stress ; Oxidative Stress - genetics ; RNA, Long Noncoding - genetics ; RNA, Long Noncoding - metabolism ; Vascular Endothelial Growth Factor A - genetics ; Vascular Endothelial Growth Factor A - metabolism ; VEGF</subject><ispartof>Redox report : communications in free radical research, 2022-12, Vol.27 (1), p.70-78</ispartof><rights>2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group 2022</rights><rights>2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group 2022 The Author(s)</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c464t-e2a43edb5680a2acee9c1a22153c9ab8a83db25a70314e7159d958f2e3c372ba3</citedby><cites>FETCH-LOGICAL-c464t-e2a43edb5680a2acee9c1a22153c9ab8a83db25a70314e7159d958f2e3c372ba3</cites><orcidid>0000-0003-3191-8300</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8928809/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8928809/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,861,882,2096,27483,27905,27906,53772,53774,59122,59123</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35285425$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Atef, Marwa Mohamed</creatorcontrib><creatorcontrib>Shafik, Noha M.</creatorcontrib><creatorcontrib>Hafez, Yasser Mostafa</creatorcontrib><creatorcontrib>Watany, Mona Mohamed</creatorcontrib><creatorcontrib>Selim, Amal</creatorcontrib><creatorcontrib>Shafik, Heba M.</creatorcontrib><creatorcontrib>Safwat El-Deeb, Omnia</creatorcontrib><title>The evolving role of long noncoding RNA HIF1A-AS2 in diabetic retinopathy: a cross-link axis between hypoxia, oxidative stress and angiogenesis via MAPK/VEGF-dependent pathway</title><title>Redox report : communications in free radical research</title><addtitle>Redox Rep</addtitle><description>Diabetic retinopathy (DR) signifies a frequent serious diabetic complication influencing retinal structure and function. Dysregulation of lncRNAs drives a wide array of human diseases especially diabetes; thus, we aimed to study lncRNA HIF1A-AS2 role and its interplay with hypoxia, oxidative stress (OS), and angiogenesis in DR.
60 DM patients in addition to 15 healthy subjects. were enrolled. LncRNA HIF1A-AS2 mRNA relative gene expression was assessed. Hypoxia inducible factor 1-alpha (HIF-1α), vascular endothelial growth factor (VEGF), mitogen activated protein kinase (MAPK), and endoglin levels were assessed. Detection of DNA damage using comet assay, and Redox status parameters were also detected.
LncRNA HIF1A-AS2 expression was significantly increased in diabetic patients with the highest levels in proliferative DR patients. Moreover, HIFα, VEGF, MAPK, and Endogolin levels were significantly higher in the diabetic patients compared to control group with the highest levels in in proliferative DR patients. Significant DNA damage in comet assay was observed to be the highest in this group.
We observed for the first time the imminent role of long noncoding RNA HIF1A-AS2 in DR throughout its stages and its interplay with hypoxia, OS, and angiogenesis via MAPK/VEGF-dependent pathway.</description><subject>angiogenesis</subject><subject>Diabetes Mellitus</subject><subject>Diabetic retinopathy</subject><subject>Diabetic Retinopathy - genetics</subject><subject>endoglin</subject><subject>HIF-1α</subject><subject>HIF1A-AS2</subject><subject>Humans</subject><subject>Hypoxia - genetics</subject><subject>Hypoxia-Inducible Factor 1, alpha Subunit - genetics</subject><subject>Hypoxia-Inducible Factor 1, alpha Subunit - metabolism</subject><subject>MAPK</subject><subject>Mitogen-Activated Protein Kinases - metabolism</subject><subject>oxidative stress</subject><subject>Oxidative Stress - genetics</subject><subject>RNA, Long Noncoding - genetics</subject><subject>RNA, Long Noncoding - metabolism</subject><subject>Vascular Endothelial Growth Factor A - genetics</subject><subject>Vascular Endothelial Growth Factor A - metabolism</subject><subject>VEGF</subject><issn>1351-0002</issn><issn>1743-2928</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>0YH</sourceid><sourceid>EIF</sourceid><sourceid>DOA</sourceid><recordid>eNp9UstuEzEUHSEQLYVPAHnJgmn9mMl4WKBGVdNGlIegsLXu2HcSl4kd7EnSfBW_iKdJK7ph4evr63PP8eNk2WtGjxmV9ISJklFK-TGnfAglpXL0JDtkVSFyXnP5NOUJkw-gg-xFjDcpE6NaPs8ORMllWfDyMPtzPUeCa9-trZuR4DskviWdTwvnnfZmKH_7PCaX0wkb5-PvnFhHjIUGe6tJSNH5JfTz7XsCRAcfY95Z94vArY0kgTaIjsy3S39r4R1J0UBv10hiHzBGAs6kMbN-hg5jallbIJ_GXz-e_Dy_mOQGl-gMup4MGhvYvsyetdBFfLWfj7Ifk_Prs8v86svF9Gx8letiVPQ5cigEmqYcSQocNGKtGXDOSqFraCRIYRpeQkUFK7BiZW3qUrYchRYVb0AcZdMdr_Fwo5bBLiBslQer7go-zBSE9AIdKt7oiqKpuDRQcN7WoiqLpuKY1KmuTeL6sONarpoFGp2uE6B7RPp4x9m5mvm1kukbJa0Twds9QfC_Vxh7tbBRY9eBQ7-Kio-ErAta1yxByx307isCtg8yjKrBOOreOGowjtobJ_W9-feMD133TkmA0x3AutaHBWx86IzqYdv50AZw2kYl_q_xF95f1Js</recordid><startdate>20221231</startdate><enddate>20221231</enddate><creator>Atef, Marwa Mohamed</creator><creator>Shafik, Noha M.</creator><creator>Hafez, Yasser Mostafa</creator><creator>Watany, Mona Mohamed</creator><creator>Selim, Amal</creator><creator>Shafik, Heba M.</creator><creator>Safwat El-Deeb, Omnia</creator><general>Taylor & Francis</general><general>Taylor & Francis Group</general><scope>0YH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0003-3191-8300</orcidid></search><sort><creationdate>20221231</creationdate><title>The evolving role of long noncoding RNA HIF1A-AS2 in diabetic retinopathy: a cross-link axis between hypoxia, oxidative stress and angiogenesis via MAPK/VEGF-dependent pathway</title><author>Atef, Marwa Mohamed ; Shafik, Noha M. ; Hafez, Yasser Mostafa ; Watany, Mona Mohamed ; Selim, Amal ; Shafik, Heba M. ; Safwat El-Deeb, Omnia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c464t-e2a43edb5680a2acee9c1a22153c9ab8a83db25a70314e7159d958f2e3c372ba3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>angiogenesis</topic><topic>Diabetes Mellitus</topic><topic>Diabetic retinopathy</topic><topic>Diabetic Retinopathy - genetics</topic><topic>endoglin</topic><topic>HIF-1α</topic><topic>HIF1A-AS2</topic><topic>Humans</topic><topic>Hypoxia - genetics</topic><topic>Hypoxia-Inducible Factor 1, alpha Subunit - genetics</topic><topic>Hypoxia-Inducible Factor 1, alpha Subunit - metabolism</topic><topic>MAPK</topic><topic>Mitogen-Activated Protein Kinases - metabolism</topic><topic>oxidative stress</topic><topic>Oxidative Stress - genetics</topic><topic>RNA, Long Noncoding - genetics</topic><topic>RNA, Long Noncoding - metabolism</topic><topic>Vascular Endothelial Growth Factor A - genetics</topic><topic>Vascular Endothelial Growth Factor A - metabolism</topic><topic>VEGF</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Atef, Marwa Mohamed</creatorcontrib><creatorcontrib>Shafik, Noha M.</creatorcontrib><creatorcontrib>Hafez, Yasser Mostafa</creatorcontrib><creatorcontrib>Watany, Mona Mohamed</creatorcontrib><creatorcontrib>Selim, Amal</creatorcontrib><creatorcontrib>Shafik, Heba M.</creatorcontrib><creatorcontrib>Safwat El-Deeb, Omnia</creatorcontrib><collection>Taylor & Francis Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Redox report : communications in free radical research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Atef, Marwa Mohamed</au><au>Shafik, Noha M.</au><au>Hafez, Yasser Mostafa</au><au>Watany, Mona Mohamed</au><au>Selim, Amal</au><au>Shafik, Heba M.</au><au>Safwat El-Deeb, Omnia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The evolving role of long noncoding RNA HIF1A-AS2 in diabetic retinopathy: a cross-link axis between hypoxia, oxidative stress and angiogenesis via MAPK/VEGF-dependent pathway</atitle><jtitle>Redox report : communications in free radical research</jtitle><addtitle>Redox Rep</addtitle><date>2022-12-31</date><risdate>2022</risdate><volume>27</volume><issue>1</issue><spage>70</spage><epage>78</epage><pages>70-78</pages><issn>1351-0002</issn><eissn>1743-2928</eissn><abstract>Diabetic retinopathy (DR) signifies a frequent serious diabetic complication influencing retinal structure and function. Dysregulation of lncRNAs drives a wide array of human diseases especially diabetes; thus, we aimed to study lncRNA HIF1A-AS2 role and its interplay with hypoxia, oxidative stress (OS), and angiogenesis in DR.
60 DM patients in addition to 15 healthy subjects. were enrolled. LncRNA HIF1A-AS2 mRNA relative gene expression was assessed. Hypoxia inducible factor 1-alpha (HIF-1α), vascular endothelial growth factor (VEGF), mitogen activated protein kinase (MAPK), and endoglin levels were assessed. Detection of DNA damage using comet assay, and Redox status parameters were also detected.
LncRNA HIF1A-AS2 expression was significantly increased in diabetic patients with the highest levels in proliferative DR patients. Moreover, HIFα, VEGF, MAPK, and Endogolin levels were significantly higher in the diabetic patients compared to control group with the highest levels in in proliferative DR patients. Significant DNA damage in comet assay was observed to be the highest in this group.
We observed for the first time the imminent role of long noncoding RNA HIF1A-AS2 in DR throughout its stages and its interplay with hypoxia, OS, and angiogenesis via MAPK/VEGF-dependent pathway.</abstract><cop>England</cop><pub>Taylor & Francis</pub><pmid>35285425</pmid><doi>10.1080/13510002.2022.2050086</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0003-3191-8300</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | angiogenesis Diabetes Mellitus Diabetic retinopathy Diabetic Retinopathy - genetics endoglin HIF-1α HIF1A-AS2 Humans Hypoxia - genetics Hypoxia-Inducible Factor 1, alpha Subunit - genetics Hypoxia-Inducible Factor 1, alpha Subunit - metabolism MAPK Mitogen-Activated Protein Kinases - metabolism oxidative stress Oxidative Stress - genetics RNA, Long Noncoding - genetics RNA, Long Noncoding - metabolism Vascular Endothelial Growth Factor A - genetics Vascular Endothelial Growth Factor A - metabolism VEGF |
| title | The evolving role of long noncoding RNA HIF1A-AS2 in diabetic retinopathy: a cross-link axis between hypoxia, oxidative stress and angiogenesis via MAPK/VEGF-dependent pathway |
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