Association of the Serum Levels of the Nucleocapsid Antigen of SARS-CoV-2 With the Diagnosis, Disease Severity, and Antibody Titers in Patients With COVID-19: A Retrospective Cross-Sectional Study
Several types of laboratory tests for COVID-19 have been established to date; however, the clinical significance of the serum SARS-CoV-2 nucleocapsid (N) antigen levels remains to be fully elucidated. In the present study, we attempted to elucidate the usefulness and clinical significance of the ser...
Gespeichert in:
| Veröffentlicht in: | Frontiers in microbiology 2021-12, Vol.12, p.791489-791489 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 791489 |
|---|---|
| container_issue | |
| container_start_page | 791489 |
| container_title | Frontiers in microbiology |
| container_volume | 12 |
| creator | Yokoyama, Rin Kurano, Makoto Nakano, Yuki Morita, Yoshifumi Ohmiya, Hiroko Kishi, Yoshiro Okada, Jun Qian, Chungen Xia, Fuzhen He, Fan Zheng, Liang Yu, Yi Mizoguchi, Miyuki Higurashi, Yoshimi Harada, Sohei Jubishi, Daisuke Okamoto, Koh Moriya, Kyoji Kodama, Tatsuhiko Yatomi, Yutaka |
| description | Several types of laboratory tests for COVID-19 have been established to date; however, the clinical significance of the serum SARS-CoV-2 nucleocapsid (N) antigen levels remains to be fully elucidated. In the present study, we attempted to elucidate the usefulness and clinical significance of the serum N antigen levels.
We measured the serum N antigen levels in 391 serum samples collected from symptomatic patients with a confirmed diagnosis of COVID-19 and 96 serum samples collected from patients with non-COVID-19, using a fully automated chemiluminescence immunoassay analyzer.
Receiver operating characteristic analysis identified the optimal cutoff value of the serum N antigen level (cutoff index, based on Youden's index) as 0.255, which yielded a sensitivity and specificity for the diagnosis of COVID-19 of 91.0 and 81.3%, respectively. The serum N antigen levels were significantly higher in the patient groups with moderate and severe COVID-19 than with mild disease. Moreover, a significant negative correlation was observed between the serum N antigen levels and the SARS-CoV-2 IgG antibody titers, especially in patients with severe COVID-19.
Serum N antigen testing might be useful both for the diagnosis of COVID-19 and for obtaining a better understanding of the clinical features of the disease. |
| doi_str_mv | 10.3389/fmicb.2021.791489 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_doaj_</sourceid><recordid>TN_cdi_pubmed_primary_34956158</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_1ef042d3b3b8472cba48affc3e847c57</doaj_id><sourcerecordid>2614759294</sourcerecordid><originalsourceid>FETCH-LOGICAL-c465t-6a8e9620ea38a2d18bc9eaedb75bedf06a19477907dbd4750eaa47e8d5f082583</originalsourceid><addsrcrecordid>eNpVUstu2zAQFIoWTZDmA3opeOwhckmKksgeChhKHwaMpojTtDeCIlc2A1l0RMqA_68fVspKgoQHcnc5O_vAJMl7gmdZxsWnZmt1PaOYklkpCOPiVXJKioKlGaZ_Xz-zT5Jz7-9wPAzTeL9NTjIm8oLk_DT5N_feaauCdR1yDQobQCvohy1awh5a_xj7OegWnFY7bw2ad8Gu4Yhfza9XaeVuU4r-2LA5Yi-tWnfOW38RTQ_Kj5R76G04XCDVTfm1Mwd0YwP0HtkO_YodQBf8xFJd3S4uUyI-ozm6htA7vwMd7B5QFW2frkbPdapFqzCYw7vkTaNaD-cP71ny-9vXm-pHurz6vqjmy1SzIg9poTiIgmJQGVfUEF5rAQpMXeY1mAYXighWlgKXpjaszCNQsRK4yRvMac6zs2Qx8Rqn7uSut1vVH6RTVh4Drl9L1QcbNyUJNJhRk9VZzVlJda0YV02jM4iuzsvI9WXi2g31FoyOw_eqfUH68qezG7l2e8kLURA-NvPxgaB39wP4ILfWa2hb1YEbvKQFiTMIKliEkgmqx-310DyVIViOYpJHMclRTHISU8z58Ly_p4xH6WT_AT0GyU0</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2614759294</pqid></control><display><type>article</type><title>Association of the Serum Levels of the Nucleocapsid Antigen of SARS-CoV-2 With the Diagnosis, Disease Severity, and Antibody Titers in Patients With COVID-19: A Retrospective Cross-Sectional Study</title><source>DOAJ Directory of Open Access Journals</source><source>PubMed Central Open Access</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>Yokoyama, Rin ; Kurano, Makoto ; Nakano, Yuki ; Morita, Yoshifumi ; Ohmiya, Hiroko ; Kishi, Yoshiro ; Okada, Jun ; Qian, Chungen ; Xia, Fuzhen ; He, Fan ; Zheng, Liang ; Yu, Yi ; Mizoguchi, Miyuki ; Higurashi, Yoshimi ; Harada, Sohei ; Jubishi, Daisuke ; Okamoto, Koh ; Moriya, Kyoji ; Kodama, Tatsuhiko ; Yatomi, Yutaka</creator><creatorcontrib>Yokoyama, Rin ; Kurano, Makoto ; Nakano, Yuki ; Morita, Yoshifumi ; Ohmiya, Hiroko ; Kishi, Yoshiro ; Okada, Jun ; Qian, Chungen ; Xia, Fuzhen ; He, Fan ; Zheng, Liang ; Yu, Yi ; Mizoguchi, Miyuki ; Higurashi, Yoshimi ; Harada, Sohei ; Jubishi, Daisuke ; Okamoto, Koh ; Moriya, Kyoji ; Kodama, Tatsuhiko ; Yatomi, Yutaka</creatorcontrib><description>Several types of laboratory tests for COVID-19 have been established to date; however, the clinical significance of the serum SARS-CoV-2 nucleocapsid (N) antigen levels remains to be fully elucidated. In the present study, we attempted to elucidate the usefulness and clinical significance of the serum N antigen levels.
We measured the serum N antigen levels in 391 serum samples collected from symptomatic patients with a confirmed diagnosis of COVID-19 and 96 serum samples collected from patients with non-COVID-19, using a fully automated chemiluminescence immunoassay analyzer.
Receiver operating characteristic analysis identified the optimal cutoff value of the serum N antigen level (cutoff index, based on Youden's index) as 0.255, which yielded a sensitivity and specificity for the diagnosis of COVID-19 of 91.0 and 81.3%, respectively. The serum N antigen levels were significantly higher in the patient groups with moderate and severe COVID-19 than with mild disease. Moreover, a significant negative correlation was observed between the serum N antigen levels and the SARS-CoV-2 IgG antibody titers, especially in patients with severe COVID-19.
Serum N antigen testing might be useful both for the diagnosis of COVID-19 and for obtaining a better understanding of the clinical features of the disease.</description><identifier>ISSN: 1664-302X</identifier><identifier>EISSN: 1664-302X</identifier><identifier>DOI: 10.3389/fmicb.2021.791489</identifier><identifier>PMID: 34956158</identifier><language>eng</language><publisher>Switzerland: Frontiers Media S.A</publisher><subject>coronavirus disease 2019 ; COVID-19 ; diagnosis ; Microbiology ; N antigen ; nucleocapsid antigen ; severity</subject><ispartof>Frontiers in microbiology, 2021-12, Vol.12, p.791489-791489</ispartof><rights>Copyright © 2021 Yokoyama, Kurano, Nakano, Morita, Ohmiya, Kishi, Okada, Qian, Xia, He, Zheng, Yu, Mizoguchi, Higurashi, Harada, Jubishi, Okamoto, Moriya, Kodama and Yatomi.</rights><rights>Copyright © 2021 Yokoyama, Kurano, Nakano, Morita, Ohmiya, Kishi, Okada, Qian, Xia, He, Zheng, Yu, Mizoguchi, Higurashi, Harada, Jubishi, Okamoto, Moriya, Kodama and Yatomi. 2021 Yokoyama, Kurano, Nakano, Morita, Ohmiya, Kishi, Okada, Qian, Xia, He, Zheng, Yu, Mizoguchi, Higurashi, Harada, Jubishi, Okamoto, Moriya, Kodama and Yatomi</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c465t-6a8e9620ea38a2d18bc9eaedb75bedf06a19477907dbd4750eaa47e8d5f082583</citedby><cites>FETCH-LOGICAL-c465t-6a8e9620ea38a2d18bc9eaedb75bedf06a19477907dbd4750eaa47e8d5f082583</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8696188/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8696188/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34956158$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yokoyama, Rin</creatorcontrib><creatorcontrib>Kurano, Makoto</creatorcontrib><creatorcontrib>Nakano, Yuki</creatorcontrib><creatorcontrib>Morita, Yoshifumi</creatorcontrib><creatorcontrib>Ohmiya, Hiroko</creatorcontrib><creatorcontrib>Kishi, Yoshiro</creatorcontrib><creatorcontrib>Okada, Jun</creatorcontrib><creatorcontrib>Qian, Chungen</creatorcontrib><creatorcontrib>Xia, Fuzhen</creatorcontrib><creatorcontrib>He, Fan</creatorcontrib><creatorcontrib>Zheng, Liang</creatorcontrib><creatorcontrib>Yu, Yi</creatorcontrib><creatorcontrib>Mizoguchi, Miyuki</creatorcontrib><creatorcontrib>Higurashi, Yoshimi</creatorcontrib><creatorcontrib>Harada, Sohei</creatorcontrib><creatorcontrib>Jubishi, Daisuke</creatorcontrib><creatorcontrib>Okamoto, Koh</creatorcontrib><creatorcontrib>Moriya, Kyoji</creatorcontrib><creatorcontrib>Kodama, Tatsuhiko</creatorcontrib><creatorcontrib>Yatomi, Yutaka</creatorcontrib><title>Association of the Serum Levels of the Nucleocapsid Antigen of SARS-CoV-2 With the Diagnosis, Disease Severity, and Antibody Titers in Patients With COVID-19: A Retrospective Cross-Sectional Study</title><title>Frontiers in microbiology</title><addtitle>Front Microbiol</addtitle><description>Several types of laboratory tests for COVID-19 have been established to date; however, the clinical significance of the serum SARS-CoV-2 nucleocapsid (N) antigen levels remains to be fully elucidated. In the present study, we attempted to elucidate the usefulness and clinical significance of the serum N antigen levels.
We measured the serum N antigen levels in 391 serum samples collected from symptomatic patients with a confirmed diagnosis of COVID-19 and 96 serum samples collected from patients with non-COVID-19, using a fully automated chemiluminescence immunoassay analyzer.
Receiver operating characteristic analysis identified the optimal cutoff value of the serum N antigen level (cutoff index, based on Youden's index) as 0.255, which yielded a sensitivity and specificity for the diagnosis of COVID-19 of 91.0 and 81.3%, respectively. The serum N antigen levels were significantly higher in the patient groups with moderate and severe COVID-19 than with mild disease. Moreover, a significant negative correlation was observed between the serum N antigen levels and the SARS-CoV-2 IgG antibody titers, especially in patients with severe COVID-19.
Serum N antigen testing might be useful both for the diagnosis of COVID-19 and for obtaining a better understanding of the clinical features of the disease.</description><subject>coronavirus disease 2019</subject><subject>COVID-19</subject><subject>diagnosis</subject><subject>Microbiology</subject><subject>N antigen</subject><subject>nucleocapsid antigen</subject><subject>severity</subject><issn>1664-302X</issn><issn>1664-302X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNpVUstu2zAQFIoWTZDmA3opeOwhckmKksgeChhKHwaMpojTtDeCIlc2A1l0RMqA_68fVspKgoQHcnc5O_vAJMl7gmdZxsWnZmt1PaOYklkpCOPiVXJKioKlGaZ_Xz-zT5Jz7-9wPAzTeL9NTjIm8oLk_DT5N_feaauCdR1yDQobQCvohy1awh5a_xj7OegWnFY7bw2ad8Gu4Yhfza9XaeVuU4r-2LA5Yi-tWnfOW38RTQ_Kj5R76G04XCDVTfm1Mwd0YwP0HtkO_YodQBf8xFJd3S4uUyI-ozm6htA7vwMd7B5QFW2frkbPdapFqzCYw7vkTaNaD-cP71ny-9vXm-pHurz6vqjmy1SzIg9poTiIgmJQGVfUEF5rAQpMXeY1mAYXighWlgKXpjaszCNQsRK4yRvMac6zs2Qx8Rqn7uSut1vVH6RTVh4Drl9L1QcbNyUJNJhRk9VZzVlJda0YV02jM4iuzsvI9WXi2g31FoyOw_eqfUH68qezG7l2e8kLURA-NvPxgaB39wP4ILfWa2hb1YEbvKQFiTMIKliEkgmqx-310DyVIViOYpJHMclRTHISU8z58Ly_p4xH6WT_AT0GyU0</recordid><startdate>20211209</startdate><enddate>20211209</enddate><creator>Yokoyama, Rin</creator><creator>Kurano, Makoto</creator><creator>Nakano, Yuki</creator><creator>Morita, Yoshifumi</creator><creator>Ohmiya, Hiroko</creator><creator>Kishi, Yoshiro</creator><creator>Okada, Jun</creator><creator>Qian, Chungen</creator><creator>Xia, Fuzhen</creator><creator>He, Fan</creator><creator>Zheng, Liang</creator><creator>Yu, Yi</creator><creator>Mizoguchi, Miyuki</creator><creator>Higurashi, Yoshimi</creator><creator>Harada, Sohei</creator><creator>Jubishi, Daisuke</creator><creator>Okamoto, Koh</creator><creator>Moriya, Kyoji</creator><creator>Kodama, Tatsuhiko</creator><creator>Yatomi, Yutaka</creator><general>Frontiers Media S.A</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20211209</creationdate><title>Association of the Serum Levels of the Nucleocapsid Antigen of SARS-CoV-2 With the Diagnosis, Disease Severity, and Antibody Titers in Patients With COVID-19: A Retrospective Cross-Sectional Study</title><author>Yokoyama, Rin ; Kurano, Makoto ; Nakano, Yuki ; Morita, Yoshifumi ; Ohmiya, Hiroko ; Kishi, Yoshiro ; Okada, Jun ; Qian, Chungen ; Xia, Fuzhen ; He, Fan ; Zheng, Liang ; Yu, Yi ; Mizoguchi, Miyuki ; Higurashi, Yoshimi ; Harada, Sohei ; Jubishi, Daisuke ; Okamoto, Koh ; Moriya, Kyoji ; Kodama, Tatsuhiko ; Yatomi, Yutaka</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c465t-6a8e9620ea38a2d18bc9eaedb75bedf06a19477907dbd4750eaa47e8d5f082583</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>coronavirus disease 2019</topic><topic>COVID-19</topic><topic>diagnosis</topic><topic>Microbiology</topic><topic>N antigen</topic><topic>nucleocapsid antigen</topic><topic>severity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yokoyama, Rin</creatorcontrib><creatorcontrib>Kurano, Makoto</creatorcontrib><creatorcontrib>Nakano, Yuki</creatorcontrib><creatorcontrib>Morita, Yoshifumi</creatorcontrib><creatorcontrib>Ohmiya, Hiroko</creatorcontrib><creatorcontrib>Kishi, Yoshiro</creatorcontrib><creatorcontrib>Okada, Jun</creatorcontrib><creatorcontrib>Qian, Chungen</creatorcontrib><creatorcontrib>Xia, Fuzhen</creatorcontrib><creatorcontrib>He, Fan</creatorcontrib><creatorcontrib>Zheng, Liang</creatorcontrib><creatorcontrib>Yu, Yi</creatorcontrib><creatorcontrib>Mizoguchi, Miyuki</creatorcontrib><creatorcontrib>Higurashi, Yoshimi</creatorcontrib><creatorcontrib>Harada, Sohei</creatorcontrib><creatorcontrib>Jubishi, Daisuke</creatorcontrib><creatorcontrib>Okamoto, Koh</creatorcontrib><creatorcontrib>Moriya, Kyoji</creatorcontrib><creatorcontrib>Kodama, Tatsuhiko</creatorcontrib><creatorcontrib>Yatomi, Yutaka</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Frontiers in microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yokoyama, Rin</au><au>Kurano, Makoto</au><au>Nakano, Yuki</au><au>Morita, Yoshifumi</au><au>Ohmiya, Hiroko</au><au>Kishi, Yoshiro</au><au>Okada, Jun</au><au>Qian, Chungen</au><au>Xia, Fuzhen</au><au>He, Fan</au><au>Zheng, Liang</au><au>Yu, Yi</au><au>Mizoguchi, Miyuki</au><au>Higurashi, Yoshimi</au><au>Harada, Sohei</au><au>Jubishi, Daisuke</au><au>Okamoto, Koh</au><au>Moriya, Kyoji</au><au>Kodama, Tatsuhiko</au><au>Yatomi, Yutaka</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Association of the Serum Levels of the Nucleocapsid Antigen of SARS-CoV-2 With the Diagnosis, Disease Severity, and Antibody Titers in Patients With COVID-19: A Retrospective Cross-Sectional Study</atitle><jtitle>Frontiers in microbiology</jtitle><addtitle>Front Microbiol</addtitle><date>2021-12-09</date><risdate>2021</risdate><volume>12</volume><spage>791489</spage><epage>791489</epage><pages>791489-791489</pages><issn>1664-302X</issn><eissn>1664-302X</eissn><abstract>Several types of laboratory tests for COVID-19 have been established to date; however, the clinical significance of the serum SARS-CoV-2 nucleocapsid (N) antigen levels remains to be fully elucidated. In the present study, we attempted to elucidate the usefulness and clinical significance of the serum N antigen levels.
We measured the serum N antigen levels in 391 serum samples collected from symptomatic patients with a confirmed diagnosis of COVID-19 and 96 serum samples collected from patients with non-COVID-19, using a fully automated chemiluminescence immunoassay analyzer.
Receiver operating characteristic analysis identified the optimal cutoff value of the serum N antigen level (cutoff index, based on Youden's index) as 0.255, which yielded a sensitivity and specificity for the diagnosis of COVID-19 of 91.0 and 81.3%, respectively. The serum N antigen levels were significantly higher in the patient groups with moderate and severe COVID-19 than with mild disease. Moreover, a significant negative correlation was observed between the serum N antigen levels and the SARS-CoV-2 IgG antibody titers, especially in patients with severe COVID-19.
Serum N antigen testing might be useful both for the diagnosis of COVID-19 and for obtaining a better understanding of the clinical features of the disease.</abstract><cop>Switzerland</cop><pub>Frontiers Media S.A</pub><pmid>34956158</pmid><doi>10.3389/fmicb.2021.791489</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1664-302X |
| ispartof | Frontiers in microbiology, 2021-12, Vol.12, p.791489-791489 |
| issn | 1664-302X 1664-302X |
| language | eng |
| recordid | cdi_pubmed_primary_34956158 |
| source | DOAJ Directory of Open Access Journals; PubMed Central Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | coronavirus disease 2019 COVID-19 diagnosis Microbiology N antigen nucleocapsid antigen severity |
| title | Association of the Serum Levels of the Nucleocapsid Antigen of SARS-CoV-2 With the Diagnosis, Disease Severity, and Antibody Titers in Patients With COVID-19: A Retrospective Cross-Sectional Study |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-07T13%3A13%3A46IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Association%20of%20the%20Serum%20Levels%20of%20the%20Nucleocapsid%20Antigen%20of%20SARS-CoV-2%20With%20the%20Diagnosis,%20Disease%20Severity,%20and%20Antibody%20Titers%20in%20Patients%20With%20COVID-19:%20A%20Retrospective%20Cross-Sectional%20Study&rft.jtitle=Frontiers%20in%20microbiology&rft.au=Yokoyama,%20Rin&rft.date=2021-12-09&rft.volume=12&rft.spage=791489&rft.epage=791489&rft.pages=791489-791489&rft.issn=1664-302X&rft.eissn=1664-302X&rft_id=info:doi/10.3389/fmicb.2021.791489&rft_dat=%3Cproquest_doaj_%3E2614759294%3C/proquest_doaj_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2614759294&rft_id=info:pmid/34956158&rft_doaj_id=oai_doaj_org_article_1ef042d3b3b8472cba48affc3e847c57&rfr_iscdi=true |