Investigation of PtSGT1 and PtSGT4 Function in Cellulose Biosynthesis in Populus tomentosa Using CRISPR/Cas9 Technology

Cellulose synthesis is a complex process in plant cells that is important for wood processing, pulping, and papermaking. Cellulose synthesis begins with the glycosylation of sitosterol by sitosterol glycosyltransferase (SGT) to produce sitosterol-glucoside (SG), which acts as the guiding primer for...

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Veröffentlicht in:	International journal of molecular sciences 2021-12, Vol.22 (24), p.13200
Hauptverfasser:	Xue, Yinxuan, Li, Siyan, Miao, Deyu, Huang, Sai, Guo, Bin, Li, Shanwen, An, Xin-Min
Format:	Artikel
Sprache:	eng
Schlagworte:	Agrobacterium - genetics Amino acids Biosynthesis Cell walls Cellulose Cellulose - biosynthesis Cloning Cloning, Molecular - methods Cotton CRISPR CRISPR-Cas Systems E coli Enzymes Expression vectors Gene Expression Regulation, Plant Genes Genetic modification Genome editing Genomes Glucose Glucose - metabolism Glucosyltransferases - genetics Glycosylation Glycosyltransferase Leaves Mutagenesis Mutants Mutation Nucleic acids Papermaking Plant cells Plant Leaves - genetics Plant Leaves - metabolism Plant Proteins - genetics Plant Stems - genetics Plant Stems - metabolism Populus - genetics Populus - metabolism Populus tomentosa Proteins Pulping Sequence analysis Signal transduction Stems Sterols Sucrose Sucrose - metabolism Transformation, Bacterial Transgenic plants Translation termination Wood - genetics Xylem
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