Lactiplantibacillus plantarum ATG-K2 Exerts an Anti-Obesity Effect in High-Fat Diet-Induced Obese Mice by Modulating the Gut Microbiome
Obesity is a major health problem. Compelling evidence supports the beneficial effects of probiotics on obesity. However, the anti-obesity effect of probiotics remains unknown. In this study, we investigated the anti-obesity effects and potential mechanisms of ATG-K2 using 3T3-L1 adipocytes and high...
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| Veröffentlicht in: | International journal of molecular sciences 2021-12, Vol.22 (23) |
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| container_title | International journal of molecular sciences |
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| creator | Lee, Young-Sil Park, Eun-Jung Park, Gun-Seok Ko, Seung-Hyun Park, Juyi Lee, You-Kyung Kim, Jong-Yeon Lee, Daeyoung Kang, Jihee Lee, Hae-Jeung |
| description | Obesity is a major health problem. Compelling evidence supports the beneficial effects of probiotics on obesity. However, the anti-obesity effect of probiotics remains unknown. In this study, we investigated the anti-obesity effects and potential mechanisms of
ATG-K2 using 3T3-L1 adipocytes and high-fat diet (HFD)-induced obese mice. 3T3-L1 cells were incubated to determine the effect of lipid accumulation with lysate of
ATG-K2. Mice were fed a normal fat diet or HFD with
ATG-K2 and Orlistat for 8 weeks.
ATG-K2 inhibited lipid accumulation in 3T3-L1 adipocytes, and reduced body weight gain, WAT weight, and adipocyte size in HFD-induced obese mice, concurrently with the downregulation of PPARγ, SREBP1c, and FAS and upregulation of PPARα, CTP1, UCP1, Prdm16, and ND5. Moreover,
ATG-K2 decreased TG, T-CHO, leptin, and TNF-α levels in the serum, with corresponding gene expression levels in the intestine.
ATG-K2 modulated the gut microbiome by increasing the abundance of the
family, which increased SCFA levels and branched SCFAs in the feces.
ATG-K2 exhibited an anti-obesity effect and anti-hyperlipidemic effect in 3T3-L1 adipocytes and HFD-induced obese mice by alleviating the inflammatory response and regulating lipid metabolism, which may be influenced by modulation of the gut microbiome and its metabolites. Therefore,
ATG-K2 can be a preventive and therapeutic agent for obesity. |
| doi_str_mv | 10.3390/ijms222312665 |
| format | Article |
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ATG-K2 using 3T3-L1 adipocytes and high-fat diet (HFD)-induced obese mice. 3T3-L1 cells were incubated to determine the effect of lipid accumulation with lysate of
ATG-K2. Mice were fed a normal fat diet or HFD with
ATG-K2 and Orlistat for 8 weeks.
ATG-K2 inhibited lipid accumulation in 3T3-L1 adipocytes, and reduced body weight gain, WAT weight, and adipocyte size in HFD-induced obese mice, concurrently with the downregulation of PPARγ, SREBP1c, and FAS and upregulation of PPARα, CTP1, UCP1, Prdm16, and ND5. Moreover,
ATG-K2 decreased TG, T-CHO, leptin, and TNF-α levels in the serum, with corresponding gene expression levels in the intestine.
ATG-K2 modulated the gut microbiome by increasing the abundance of the
family, which increased SCFA levels and branched SCFAs in the feces.
ATG-K2 exhibited an anti-obesity effect and anti-hyperlipidemic effect in 3T3-L1 adipocytes and HFD-induced obese mice by alleviating the inflammatory response and regulating lipid metabolism, which may be influenced by modulation of the gut microbiome and its metabolites. Therefore,
ATG-K2 can be a preventive and therapeutic agent for obesity.</description><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms222312665</identifier><identifier>PMID: 34884471</identifier><language>eng</language><publisher>Switzerland</publisher><subject>3T3-L1 Cells ; Animals ; Biological Factors - analysis ; Body Weight ; Diet, High-Fat - adverse effects ; Disease Models, Animal ; Gastrointestinal Microbiome - drug effects ; Gene Expression Regulation ; Lactobacillaceae - chemistry ; Lactobacillaceae - physiology ; Mice ; Mice, Obese ; Obesity - chemically induced ; Obesity - diet therapy ; Obesity - genetics ; Probiotics - administration & dosage ; Probiotics - pharmacology</subject><ispartof>International journal of molecular sciences, 2021-12, Vol.22 (23)</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><orcidid>0000-0002-2778-1834 ; 0000-0001-6757-3127 ; 0000-0001-8353-3619 ; 0000-0002-5377-2819</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34884471$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lee, Young-Sil</creatorcontrib><creatorcontrib>Park, Eun-Jung</creatorcontrib><creatorcontrib>Park, Gun-Seok</creatorcontrib><creatorcontrib>Ko, Seung-Hyun</creatorcontrib><creatorcontrib>Park, Juyi</creatorcontrib><creatorcontrib>Lee, You-Kyung</creatorcontrib><creatorcontrib>Kim, Jong-Yeon</creatorcontrib><creatorcontrib>Lee, Daeyoung</creatorcontrib><creatorcontrib>Kang, Jihee</creatorcontrib><creatorcontrib>Lee, Hae-Jeung</creatorcontrib><title>Lactiplantibacillus plantarum ATG-K2 Exerts an Anti-Obesity Effect in High-Fat Diet-Induced Obese Mice by Modulating the Gut Microbiome</title><title>International journal of molecular sciences</title><addtitle>Int J Mol Sci</addtitle><description>Obesity is a major health problem. Compelling evidence supports the beneficial effects of probiotics on obesity. However, the anti-obesity effect of probiotics remains unknown. In this study, we investigated the anti-obesity effects and potential mechanisms of
ATG-K2 using 3T3-L1 adipocytes and high-fat diet (HFD)-induced obese mice. 3T3-L1 cells were incubated to determine the effect of lipid accumulation with lysate of
ATG-K2. Mice were fed a normal fat diet or HFD with
ATG-K2 and Orlistat for 8 weeks.
ATG-K2 inhibited lipid accumulation in 3T3-L1 adipocytes, and reduced body weight gain, WAT weight, and adipocyte size in HFD-induced obese mice, concurrently with the downregulation of PPARγ, SREBP1c, and FAS and upregulation of PPARα, CTP1, UCP1, Prdm16, and ND5. Moreover,
ATG-K2 decreased TG, T-CHO, leptin, and TNF-α levels in the serum, with corresponding gene expression levels in the intestine.
ATG-K2 modulated the gut microbiome by increasing the abundance of the
family, which increased SCFA levels and branched SCFAs in the feces.
ATG-K2 exhibited an anti-obesity effect and anti-hyperlipidemic effect in 3T3-L1 adipocytes and HFD-induced obese mice by alleviating the inflammatory response and regulating lipid metabolism, which may be influenced by modulation of the gut microbiome and its metabolites. Therefore,
ATG-K2 can be a preventive and therapeutic agent for obesity.</description><subject>3T3-L1 Cells</subject><subject>Animals</subject><subject>Biological Factors - analysis</subject><subject>Body Weight</subject><subject>Diet, High-Fat - adverse effects</subject><subject>Disease Models, Animal</subject><subject>Gastrointestinal Microbiome - drug effects</subject><subject>Gene Expression Regulation</subject><subject>Lactobacillaceae - chemistry</subject><subject>Lactobacillaceae - physiology</subject><subject>Mice</subject><subject>Mice, Obese</subject><subject>Obesity - chemically induced</subject><subject>Obesity - diet therapy</subject><subject>Obesity - genetics</subject><subject>Probiotics - administration & dosage</subject><subject>Probiotics - pharmacology</subject><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kM1uwjAQhK1KVaG0x16rfQG3sR0n9hFR_lQQF-5o42zAKAlR4kjlCfrahdKeRjPzaQ7D2IuI3pSy0bs_Vp2UUgmZJPqODUUsJY-iJB2wx647RpFUUtsHNlCxMXGciiH7XqELvimxDj5D58uy7-DXYttXMN7O-aeE6Re1oQOsYXzh-CajzoczTIuCXABfw8LvD3yGAT48Bb6s895RDleOYO0dQXaG9SnvSwy-3kM4EMz7cK3aU-ZPFT2x-wLLjp7_dMS2s-l2suCrzXw5Ga94Y1Xg2iZGS6tUJiLpkKwRKNChMDolTSZxqbDWqNyQxOySYpFIiyjTyxVaCzVir7fZps8qyndN6ytsz7v_Q9QPJwxgpw</recordid><startdate>20211201</startdate><enddate>20211201</enddate><creator>Lee, Young-Sil</creator><creator>Park, Eun-Jung</creator><creator>Park, Gun-Seok</creator><creator>Ko, Seung-Hyun</creator><creator>Park, Juyi</creator><creator>Lee, You-Kyung</creator><creator>Kim, Jong-Yeon</creator><creator>Lee, Daeyoung</creator><creator>Kang, Jihee</creator><creator>Lee, Hae-Jeung</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><orcidid>https://orcid.org/0000-0002-2778-1834</orcidid><orcidid>https://orcid.org/0000-0001-6757-3127</orcidid><orcidid>https://orcid.org/0000-0001-8353-3619</orcidid><orcidid>https://orcid.org/0000-0002-5377-2819</orcidid></search><sort><creationdate>20211201</creationdate><title>Lactiplantibacillus plantarum ATG-K2 Exerts an Anti-Obesity Effect in High-Fat Diet-Induced Obese Mice by Modulating the Gut Microbiome</title><author>Lee, Young-Sil ; Park, Eun-Jung ; Park, Gun-Seok ; Ko, Seung-Hyun ; Park, Juyi ; Lee, You-Kyung ; Kim, Jong-Yeon ; Lee, Daeyoung ; Kang, Jihee ; Lee, Hae-Jeung</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p93t-596852933b102cae981a1aca1857e5e86c719983d8e2ab185af629aa271425513</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>3T3-L1 Cells</topic><topic>Animals</topic><topic>Biological Factors - analysis</topic><topic>Body Weight</topic><topic>Diet, High-Fat - adverse effects</topic><topic>Disease Models, Animal</topic><topic>Gastrointestinal Microbiome - drug effects</topic><topic>Gene Expression Regulation</topic><topic>Lactobacillaceae - chemistry</topic><topic>Lactobacillaceae - physiology</topic><topic>Mice</topic><topic>Mice, Obese</topic><topic>Obesity - chemically induced</topic><topic>Obesity - diet therapy</topic><topic>Obesity - genetics</topic><topic>Probiotics - administration & dosage</topic><topic>Probiotics - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, Young-Sil</creatorcontrib><creatorcontrib>Park, Eun-Jung</creatorcontrib><creatorcontrib>Park, Gun-Seok</creatorcontrib><creatorcontrib>Ko, Seung-Hyun</creatorcontrib><creatorcontrib>Park, Juyi</creatorcontrib><creatorcontrib>Lee, You-Kyung</creatorcontrib><creatorcontrib>Kim, Jong-Yeon</creatorcontrib><creatorcontrib>Lee, Daeyoung</creatorcontrib><creatorcontrib>Kang, Jihee</creatorcontrib><creatorcontrib>Lee, Hae-Jeung</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>International journal of molecular sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, Young-Sil</au><au>Park, Eun-Jung</au><au>Park, Gun-Seok</au><au>Ko, Seung-Hyun</au><au>Park, Juyi</au><au>Lee, You-Kyung</au><au>Kim, Jong-Yeon</au><au>Lee, Daeyoung</au><au>Kang, Jihee</au><au>Lee, Hae-Jeung</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Lactiplantibacillus plantarum ATG-K2 Exerts an Anti-Obesity Effect in High-Fat Diet-Induced Obese Mice by Modulating the Gut Microbiome</atitle><jtitle>International journal of molecular sciences</jtitle><addtitle>Int J Mol Sci</addtitle><date>2021-12-01</date><risdate>2021</risdate><volume>22</volume><issue>23</issue><eissn>1422-0067</eissn><abstract>Obesity is a major health problem. Compelling evidence supports the beneficial effects of probiotics on obesity. However, the anti-obesity effect of probiotics remains unknown. In this study, we investigated the anti-obesity effects and potential mechanisms of
ATG-K2 using 3T3-L1 adipocytes and high-fat diet (HFD)-induced obese mice. 3T3-L1 cells were incubated to determine the effect of lipid accumulation with lysate of
ATG-K2. Mice were fed a normal fat diet or HFD with
ATG-K2 and Orlistat for 8 weeks.
ATG-K2 inhibited lipid accumulation in 3T3-L1 adipocytes, and reduced body weight gain, WAT weight, and adipocyte size in HFD-induced obese mice, concurrently with the downregulation of PPARγ, SREBP1c, and FAS and upregulation of PPARα, CTP1, UCP1, Prdm16, and ND5. Moreover,
ATG-K2 decreased TG, T-CHO, leptin, and TNF-α levels in the serum, with corresponding gene expression levels in the intestine.
ATG-K2 modulated the gut microbiome by increasing the abundance of the
family, which increased SCFA levels and branched SCFAs in the feces.
ATG-K2 exhibited an anti-obesity effect and anti-hyperlipidemic effect in 3T3-L1 adipocytes and HFD-induced obese mice by alleviating the inflammatory response and regulating lipid metabolism, which may be influenced by modulation of the gut microbiome and its metabolites. Therefore,
ATG-K2 can be a preventive and therapeutic agent for obesity.</abstract><cop>Switzerland</cop><pmid>34884471</pmid><doi>10.3390/ijms222312665</doi><orcidid>https://orcid.org/0000-0002-2778-1834</orcidid><orcidid>https://orcid.org/0000-0001-6757-3127</orcidid><orcidid>https://orcid.org/0000-0001-8353-3619</orcidid><orcidid>https://orcid.org/0000-0002-5377-2819</orcidid></addata></record> |
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| subjects | 3T3-L1 Cells Animals Biological Factors - analysis Body Weight Diet, High-Fat - adverse effects Disease Models, Animal Gastrointestinal Microbiome - drug effects Gene Expression Regulation Lactobacillaceae - chemistry Lactobacillaceae - physiology Mice Mice, Obese Obesity - chemically induced Obesity - diet therapy Obesity - genetics Probiotics - administration & dosage Probiotics - pharmacology |
| title | Lactiplantibacillus plantarum ATG-K2 Exerts an Anti-Obesity Effect in High-Fat Diet-Induced Obese Mice by Modulating the Gut Microbiome |
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