Fluorescent Actin Filaments Move on Myosin Fixed to a Glass Surface

Single actin filaments stabilized with fluorescent phalloidin exhibit ATP-dependent movement on myosin filaments fixed to a surface. At pH 7.4 and 24 degrees C, the rates of movement average 3-4 μ m/s with skeletal muscle myosin and 1-2 μ m/s with Dictyostelium myosin. These rates are very similar t...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1986-09, Vol.83 (17), p.6272-6276
Hauptverfasser:	Kron, Stephen J., Spudich, James A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Actins Actins - physiology Actomyosin - physiology Adenosine triphosphatases Adenosine Triphosphate - metabolism Animals Biochemistry Biological and medical sciences Cell membranes Conformational dynamics in molecular biology Dictyostelium Fundamental and applied biological sciences. Psychology Glass Hydrogen-Ion Concentration In Vitro Techniques Microfilaments Microscopy, Fluorescence Microtubules Molecular biophysics Molecules Muscle Contraction Myosins - physiology Phalloidine Plant cells Rabbits Rhodamines Skeletal muscle Velocity
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