Characterization of Protocatechuate 4,5-Dioxygenase from Pseudarthrobacter phenanthrenivorans Sphe3 and In Situ Reaction Monitoring in the NMR Tube

The current study aims at the functional and kinetic characterization of protocatechuate (PCA) 4,5-dioxygenase (PcaA) from Pseudarthrobacter phenanthrenivorans Sphe3. This is the first single subunit Type II dioxygenase characterized in Actinobacteria. RT-PCR analysis demonstrated that pcaA and the...

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Veröffentlicht in:	International journal of molecular sciences 2021-09, Vol.22 (17), p.9647, Article 9647
Hauptverfasser:	Tsagogiannis, Epameinondas, Vandera, Elpiniki, Primikyri, Alexandra, Asimakoula, Stamatia, Tzakos, Andreas G., Gerothanassis, Ioannis P., Koukkou, Anna-Irini
Format:	Artikel
Sprache:	eng
Schlagworte:	Actinobacteria Amino acids Bacterial Proteins - classification Bacterial Proteins - genetics Bacterial Proteins - metabolism Biocatalysis Biocatalysts Biochemistry & Molecular Biology Biodegradation Bioremediation Biotransformation Chemistry Chemistry, Multidisciplinary Dioxygenases - chemistry Dioxygenases - genetics Dioxygenases - metabolism Enzymes extradiol dioxygenases Gallic Acid - chemistry Gallic Acid - metabolism Gene Expression Regulation, Bacterial Gene Expression Regulation, Enzymologic Genes Genomes Glucose Hydrocarbons Hydrogen-Ion Concentration in situ biotransformation monitoring Kinetics Life Sciences & Biomedicine Magnetic Resonance Spectroscopy - methods Micrococcaceae - enzymology Micrococcaceae - genetics Molecular Structure Monitoring NMR NMR bioreactor Nuclear magnetic resonance Phenanthrene Phenanthrenes - chemistry Phenanthrenes - metabolism Phylogenetics Phylogeny Physical Sciences Polymerase chain reaction Proteins protocatechuate (PCA) 4,5-dioxygenase Protocatechuate 4,5-dioxygenase Pseudarthrobacter phenanthrenivorans Sphe3 Reaction products Reverse Transcriptase Polymerase Chain Reaction - methods Science & Technology Stereoisomerism Substrate Specificity Transcription
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