Oceaniglobus trochenteri sp. nov., isolated from the gut microflora of top shell (Trochus maculatus Linnaeus)

A Gram-stain negative, non-flagellated, beige-pigmented, circular, catalase-positive, oxidase-positive bacterium, designated G4 T , was isolated from gut microflora of top shell ( Trochus maculatus Linnaeus ) collected from Diwanggong market, Weihai, People’s Republic of China. The novel isolate was able to grow at 4–42 °C (optimum 25–33 °C), pH 7.0–9.0 (optimum 6.5–7.0) and with 0.0–11.0% NaCl (optimum 2.0–3.0%, w/v). Analysis of 16S rRNA gene sequence revealed that strain G4 T shared the highest 16S rRNA gene sequence similarities with Oceaniglobus ichthyenteri YLY08 T (96.6%), followed by Oceaniglobus indicus 1-19b T (95.3%). The genome of strain G4 T , with 32 assembled contigs, was 4.5 Mb long with a G+C content of 65.3 mol%. DNA–DNA hybridization values of the isolate against the closely related type strains were far below the 70% limit for species delineation. The average amino acid identity, average nucleotide identity and digital DNA–DNA genome hybridization relatedness between strain G4 T and the closely related members of the genus Oceaniglobus , Oceaniglobus indicus 1-19b T and Oceaniglobus ichthyenteri YLY08 T were 71.3, 76.4 and 20.0%, and 75.0, 76.3 and 19.4%. The major cellular fatty acid was summed feature 8 (C 18:1 ω 7 c and/or C 18:1 ω 6 c ). The sole respiratory quinone was Q-10. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and phosphatidyldimethylethanolamine. The results of phenotypical, phylogenetic and biochemical analyses indicated that strain G4 T represents a novel species in genus Oceaniglobus within the family Rhodobacteraceae , for which the name Oceaniglobus trochenteri sp. nov. is proposed. The type strain is G4 T (= MCCC 1K04356 T = KCTC 82506 T ).