Active-Site Models of Streptococcus pyogenes Cas9 in DNA Cleavage State

CRISPR-Cas9 is a powerful tool for target genome editing in living cells. Significant advances have been made to understand how this system cleaves target DNA. HNH is a nuclease domain, which shares structural similarity with the HNH endonuclease characterzied by a beta-beta-alpha-metal fold. Theref...

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Veröffentlicht in:	Frontiers in molecular biosciences 2021-04, Vol.8, p.653262-653262
Hauptverfasser:	Tang, Honghai, Yuan, Hui, Du, Wenhao, Li, Gan, Xue, Dongmei, Huang, Qiang
Format:	Artikel
Sprache:	eng
Schlagworte:	CRISPR-Cas9 DNA cleavage mechanism Gene editing Molecular Biosciences Molecular dynamics off-target effects
Online-Zugang:	Volltext
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