Voltage clamp studies of fertilization in sea urchin eggs: II. Current patterns in relation to sperm entry, nonentry, and activation
Following attachment of a sperm to the surface of a sea urchin egg clamped at a membrane potential ( V m) more positive than +17 mV, no changes in membrane conductance can be detected, the sperm does not enter the egg, and no morphological changes can be detected. At V m from +17 to −100 mV three ch...
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| Veröffentlicht in: | Developmental biology 1988, Vol.128 (2), p.305-323 |
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| creator | Lynn, John W. McCulloh, David H. Chambers, Edward L. |
| description | Following attachment of a sperm to the surface of a sea urchin egg clamped at a membrane potential (
V
m) more positive than +17 mV, no changes in membrane conductance can be detected, the sperm does not enter the egg, and no morphological changes can be detected. At V
m from +17 to −100 mV three characteristically different types of current profiles are observed: Type I are activation currents in eggs penetrated by a sperm. These have three phases, which occur in all eggs clamped at
V
m from +17 to −20 mV and in decreasing percentages at clamped
V
m more negative than −20 mV (to −75 mV). Complete fertilization envelopes are elevated, relatively large mound-shaped fertilization cones form, and the eggs develop to normal embryos. Type II are sperm transient currents in eggs not penetrated by a sperm, the eggs otherwise remaining in the unfertilized state. These transients are simpler and shorter than type I currents, and are observed only at clamped
V
m more negative than −20 mV. Type III are modified activation currents in eggs not penetrated by a sperm. These have three phases, are observed only at clamped
V
m more negative than −20 mV, and are the only type of activation current seen at clamped
V
m more negative than −75 mV. Complete fertilization envelopes are elevated, the fertilization cones are small and filament-like, and the eggs fail to cleave. We conclude that (a) the sperm transient currents (type II) and phase 1 of the activation currents (types I and III) are similar events generated by a sperm-initiated localized conductance increase, (b) the abrupt decrease of current which terminates the sperm transients and phase 1 of type III currents results from a turnoff of the sperm-induced conductance increase and signals that the sperm will not enter the egg, and (c) the occurrence of phase 2 during an electrophysiological response induced by a sperm indicates that the egg is activating. |
| doi_str_mv | 10.1016/0012-1606(88)90294-1 |
| format | Article |
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V
m) more positive than +17 mV, no changes in membrane conductance can be detected, the sperm does not enter the egg, and no morphological changes can be detected. At V
m from +17 to −100 mV three characteristically different types of current profiles are observed: Type I are activation currents in eggs penetrated by a sperm. These have three phases, which occur in all eggs clamped at
V
m from +17 to −20 mV and in decreasing percentages at clamped
V
m more negative than −20 mV (to −75 mV). Complete fertilization envelopes are elevated, relatively large mound-shaped fertilization cones form, and the eggs develop to normal embryos. Type II are sperm transient currents in eggs not penetrated by a sperm, the eggs otherwise remaining in the unfertilized state. These transients are simpler and shorter than type I currents, and are observed only at clamped
V
m more negative than −20 mV. Type III are modified activation currents in eggs not penetrated by a sperm. These have three phases, are observed only at clamped
V
m more negative than −20 mV, and are the only type of activation current seen at clamped
V
m more negative than −75 mV. Complete fertilization envelopes are elevated, the fertilization cones are small and filament-like, and the eggs fail to cleave. We conclude that (a) the sperm transient currents (type II) and phase 1 of the activation currents (types I and III) are similar events generated by a sperm-initiated localized conductance increase, (b) the abrupt decrease of current which terminates the sperm transients and phase 1 of type III currents results from a turnoff of the sperm-induced conductance increase and signals that the sperm will not enter the egg, and (c) the occurrence of phase 2 during an electrophysiological response induced by a sperm indicates that the egg is activating.</description><identifier>ISSN: 0012-1606</identifier><identifier>EISSN: 1095-564X</identifier><identifier>DOI: 10.1016/0012-1606(88)90294-1</identifier><identifier>PMID: 3396763</identifier><identifier>CODEN: DEBIAO</identifier><language>eng</language><publisher>Amsterdam: Elsevier Inc</publisher><subject>Animals ; Biological and medical sciences ; Electrophysiology ; Female ; Fertilization ; Fundamental and applied biological sciences. Psychology ; Hemodynamics. Rheology ; Male ; Oocytes - physiology ; Reference Values ; Sea Urchins - physiology ; Sperm-Ovum Interactions ; Spermatozoa - physiology ; Vertebrates: cardiovascular system</subject><ispartof>Developmental biology, 1988, Vol.128 (2), p.305-323</ispartof><rights>1988</rights><rights>1988 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0012-1606(88)90294-1$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,4024,27923,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7824663$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3396763$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lynn, John W.</creatorcontrib><creatorcontrib>McCulloh, David H.</creatorcontrib><creatorcontrib>Chambers, Edward L.</creatorcontrib><title>Voltage clamp studies of fertilization in sea urchin eggs: II. Current patterns in relation to sperm entry, nonentry, and activation</title><title>Developmental biology</title><addtitle>Dev Biol</addtitle><description>Following attachment of a sperm to the surface of a sea urchin egg clamped at a membrane potential (
V
m) more positive than +17 mV, no changes in membrane conductance can be detected, the sperm does not enter the egg, and no morphological changes can be detected. At V
m from +17 to −100 mV three characteristically different types of current profiles are observed: Type I are activation currents in eggs penetrated by a sperm. These have three phases, which occur in all eggs clamped at
V
m from +17 to −20 mV and in decreasing percentages at clamped
V
m more negative than −20 mV (to −75 mV). Complete fertilization envelopes are elevated, relatively large mound-shaped fertilization cones form, and the eggs develop to normal embryos. Type II are sperm transient currents in eggs not penetrated by a sperm, the eggs otherwise remaining in the unfertilized state. These transients are simpler and shorter than type I currents, and are observed only at clamped
V
m more negative than −20 mV. Type III are modified activation currents in eggs not penetrated by a sperm. These have three phases, are observed only at clamped
V
m more negative than −20 mV, and are the only type of activation current seen at clamped
V
m more negative than −75 mV. Complete fertilization envelopes are elevated, the fertilization cones are small and filament-like, and the eggs fail to cleave. We conclude that (a) the sperm transient currents (type II) and phase 1 of the activation currents (types I and III) are similar events generated by a sperm-initiated localized conductance increase, (b) the abrupt decrease of current which terminates the sperm transients and phase 1 of type III currents results from a turnoff of the sperm-induced conductance increase and signals that the sperm will not enter the egg, and (c) the occurrence of phase 2 during an electrophysiological response induced by a sperm indicates that the egg is activating.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Electrophysiology</subject><subject>Female</subject><subject>Fertilization</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hemodynamics. Rheology</subject><subject>Male</subject><subject>Oocytes - physiology</subject><subject>Reference Values</subject><subject>Sea Urchins - physiology</subject><subject>Sperm-Ovum Interactions</subject><subject>Spermatozoa - physiology</subject><subject>Vertebrates: cardiovascular system</subject><issn>0012-1606</issn><issn>1095-564X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kU1LAzEQhoMotVb_gUIOHhTcmmx2s1kPghQ_CgUvKt5Cmp3UyDa7JNlCPfvD3X7gaQbeZ4Z550XonJIxJZTfEkLThHLCr4S4LklaZgk9QENKyjzJefZ5iIb_yDE6CeGbEMKEYAM0YKzkBWdD9PvR1FEtAOtaLVscYldZCLgx2ICPtrY_KtrGYetwAIU7r7_6FhaLcIen0zGedN6Di7hVMYJ3YQN6qHdDscGhBb_EPeHXN9g1bt8pV2Glo11twVN0ZFQd4GxfR-j96fFt8pLMXp-nk4dZArTIYkLT3GhaCt27qAAo5aZ3MYdCaVMykhlusrxXCTeQ0d57KYqsMnPDtKGlUWyELnZ7226-hEq23i6VX8v9N3r9cq-roFVtvHLahn-sEGnGt9j9DoP-1pUFL4O24DRU1oOOsmqspERuQpKbBOQmASmE3IYkKfsD_LGE0w</recordid><startdate>1988</startdate><enddate>1988</enddate><creator>Lynn, John W.</creator><creator>McCulloh, David H.</creator><creator>Chambers, Edward L.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>1988</creationdate><title>Voltage clamp studies of fertilization in sea urchin eggs: II. Current patterns in relation to sperm entry, nonentry, and activation</title><author>Lynn, John W. ; McCulloh, David H. ; Chambers, Edward L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e174t-125fc198c038dee116f967be7acf9304f6f458c006fe410019874dfbf3cf19fa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Electrophysiology</topic><topic>Female</topic><topic>Fertilization</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hemodynamics. Rheology</topic><topic>Male</topic><topic>Oocytes - physiology</topic><topic>Reference Values</topic><topic>Sea Urchins - physiology</topic><topic>Sperm-Ovum Interactions</topic><topic>Spermatozoa - physiology</topic><topic>Vertebrates: cardiovascular system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lynn, John W.</creatorcontrib><creatorcontrib>McCulloh, David H.</creatorcontrib><creatorcontrib>Chambers, Edward L.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>Developmental biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lynn, John W.</au><au>McCulloh, David H.</au><au>Chambers, Edward L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Voltage clamp studies of fertilization in sea urchin eggs: II. Current patterns in relation to sperm entry, nonentry, and activation</atitle><jtitle>Developmental biology</jtitle><addtitle>Dev Biol</addtitle><date>1988</date><risdate>1988</risdate><volume>128</volume><issue>2</issue><spage>305</spage><epage>323</epage><pages>305-323</pages><issn>0012-1606</issn><eissn>1095-564X</eissn><coden>DEBIAO</coden><abstract>Following attachment of a sperm to the surface of a sea urchin egg clamped at a membrane potential (
V
m) more positive than +17 mV, no changes in membrane conductance can be detected, the sperm does not enter the egg, and no morphological changes can be detected. At V
m from +17 to −100 mV three characteristically different types of current profiles are observed: Type I are activation currents in eggs penetrated by a sperm. These have three phases, which occur in all eggs clamped at
V
m from +17 to −20 mV and in decreasing percentages at clamped
V
m more negative than −20 mV (to −75 mV). Complete fertilization envelopes are elevated, relatively large mound-shaped fertilization cones form, and the eggs develop to normal embryos. Type II are sperm transient currents in eggs not penetrated by a sperm, the eggs otherwise remaining in the unfertilized state. These transients are simpler and shorter than type I currents, and are observed only at clamped
V
m more negative than −20 mV. Type III are modified activation currents in eggs not penetrated by a sperm. These have three phases, are observed only at clamped
V
m more negative than −20 mV, and are the only type of activation current seen at clamped
V
m more negative than −75 mV. Complete fertilization envelopes are elevated, the fertilization cones are small and filament-like, and the eggs fail to cleave. We conclude that (a) the sperm transient currents (type II) and phase 1 of the activation currents (types I and III) are similar events generated by a sperm-initiated localized conductance increase, (b) the abrupt decrease of current which terminates the sperm transients and phase 1 of type III currents results from a turnoff of the sperm-induced conductance increase and signals that the sperm will not enter the egg, and (c) the occurrence of phase 2 during an electrophysiological response induced by a sperm indicates that the egg is activating.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><pmid>3396763</pmid><doi>10.1016/0012-1606(88)90294-1</doi><tpages>19</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Developmental biology, 1988, Vol.128 (2), p.305-323 |
| issn | 0012-1606 1095-564X |
| language | eng |
| recordid | cdi_pubmed_primary_3396763 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Biological and medical sciences Electrophysiology Female Fertilization Fundamental and applied biological sciences. Psychology Hemodynamics. Rheology Male Oocytes - physiology Reference Values Sea Urchins - physiology Sperm-Ovum Interactions Spermatozoa - physiology Vertebrates: cardiovascular system |
| title | Voltage clamp studies of fertilization in sea urchin eggs: II. Current patterns in relation to sperm entry, nonentry, and activation |
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