Iron-binding cellular profile of transferrin using label-free Raman hyperspectral imaging and singular value decomposition (SVD)

Serum transferrin (Tf) is the essential iron transport protein in the body. Transferrin is responsible for the sequestration of free iron in serum and the delivery of iron throughout the body and into cells, where iron is released inside a mildly acidified endosome. Altered iron distributions are as...

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Veröffentlicht in:Free radical biology & medicine 2021-06, Vol.169, p.416-424
Hauptverfasser: Tubbesing, Kate, Khoo, Ting Chean, Bahreini Jangjoo, Shahab, Sharikova, Anna, Barroso, Margarida, Khmaladze, Alexander
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container_start_page 416
container_title Free radical biology & medicine
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creator Tubbesing, Kate
Khoo, Ting Chean
Bahreini Jangjoo, Shahab
Sharikova, Anna
Barroso, Margarida
Khmaladze, Alexander
description Serum transferrin (Tf) is the essential iron transport protein in the body. Transferrin is responsible for the sequestration of free iron in serum and the delivery of iron throughout the body and into cells, where iron is released inside a mildly acidified endosome. Altered iron distributions are associated with diseases such as iron-overload, cancer, and cardiovascular disease. The presence of free iron is linked to deleterious redox reactions, inside and outside cells and organelles. As Tf iron release is pH dependent, any changes in intraorganelle and extracellular pH, often associated with disease progression, could inhibit normal iron delivery or accelerate iron release in the wrong compartment. However, imaging approaches to monitor changes in the iron-bound state of Tf are lacking. Recently, Raman spectroscopy has been shown to measure iron-bound forms of Tf in solution, intact cells and tissue samples. Here, a biochemical Raman assay has been developed to identify iron-release from Tf following modification of chemical environment. Quantitative singular value decomposition (SVD) method has been applied to discriminate between iron-bound Tf samples during endocytic trafficking in intact cancer cells subjected to Raman hyperspectral confocal imaging. We demonstrate the strength of the SVD method to monitor pH-induced Tf iron-release using Raman hyperspectral imaging, providing the redox biology field with a novel tool that facilitates subcellular investigation of the iron-binding profile of transferrin in various disease models. [Display omitted] •Raman spectroscopy differentiates iron-bound and iron-free transferrin (Tf),key for investigating iron transport regulation.•Raman hyperspectral imaging can evaluate pH-induced Tf iron release in the endocytic compartments of intact, unlabeled cells.•Singular value decomposition enables complex Raman data analysis by semi-automated unbiased discrimination of Tf iron-binding profiles.
doi_str_mv 10.1016/j.freeradbiomed.2021.04.030
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Quantitative singular value decomposition (SVD) method has been applied to discriminate between iron-bound Tf samples during endocytic trafficking in intact cancer cells subjected to Raman hyperspectral confocal imaging. We demonstrate the strength of the SVD method to monitor pH-induced Tf iron-release using Raman hyperspectral imaging, providing the redox biology field with a novel tool that facilitates subcellular investigation of the iron-binding profile of transferrin in various disease models. 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Quantitative singular value decomposition (SVD) method has been applied to discriminate between iron-bound Tf samples during endocytic trafficking in intact cancer cells subjected to Raman hyperspectral confocal imaging. We demonstrate the strength of the SVD method to monitor pH-induced Tf iron-release using Raman hyperspectral imaging, providing the redox biology field with a novel tool that facilitates subcellular investigation of the iron-binding profile of transferrin in various disease models. 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subjects Biochemistry & Molecular Biology
Cancer
Endocrinology & Metabolism
Endosomes - metabolism
Hyperspectral Imaging
Iron
Iron - metabolism
Life Sciences & Biomedicine
Raman hyperspectral imaging
Receptors, Transferrin
Redox biology
Science & Technology
Transferrin
title Iron-binding cellular profile of transferrin using label-free Raman hyperspectral imaging and singular value decomposition (SVD)
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