Identification of PDGFRα-positive interstitial cells in the distal segment of the murine vas deferens
Platelet-derived growth factor receptor-α (PDGFRα)-positive interstitial cells (ICs) are widely distributed in various organs and may be involved in the motility of various tubular organs. We, for the first time, aimed to investigate the distribution, immunohistochemical characteristics, and ultrast...
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| Veröffentlicht in: | Scientific reports 2021-04, Vol.11 (1), p.7553-7553, Article 7553 |
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| creator | Hiroshige, Tasuku Uemura, Kei-Ichiro Hirashima, Shingo Hino, Kiyosato Togo, Akinobu Ohta, Keisuke Igawa, Tsukasa Nakamura, Kei-Ichiro |
| description | Platelet-derived growth factor receptor-α (PDGFRα)-positive interstitial cells (ICs) are widely distributed in various organs and may be involved in the motility of various tubular organs. We, for the first time, aimed to investigate the distribution, immunohistochemical characteristics, and ultrastructure of PDGFRα-positive ICs in murine vas deferens, using confocal laser scanning microscopy, transmission electron microscopy (TEM), and immuno-electron microscopy (immuno-EM). For immunofluorescence, we used antibodies against PDGFRα and other markers of ICs. PDGFRα-positive ICs were distributed widely in the lamina propria, smooth muscles, and serosal layers. Although most PDGFRα-positive ICs labeled CD34, they did not label CD34 in the subepithelial layers. Additionally, PDGFRα-positive ICs were in close proximity to each other, as also to the surrounding cells. TEM and immuno-EM findings revealed that PDGFRα-positive ICs established close physical interactions with adjacent ICs. Extracellular vesicles were also detected around the PDGFRα-positive ICs. Our morphological findings suggest that PDGFRα-positive ICs may have several subpopulations, which can play an important role in intercellular signaling via direct contact with the IC network and the extracellular vesicles in the murine vas deferens. Further investigation on PDGFRα-positive ICs in the vas deferens may lead to understanding the vas deferens mortility. |
| doi_str_mv | 10.1038/s41598-021-87049-6 |
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We, for the first time, aimed to investigate the distribution, immunohistochemical characteristics, and ultrastructure of PDGFRα-positive ICs in murine vas deferens, using confocal laser scanning microscopy, transmission electron microscopy (TEM), and immuno-electron microscopy (immuno-EM). For immunofluorescence, we used antibodies against PDGFRα and other markers of ICs. PDGFRα-positive ICs were distributed widely in the lamina propria, smooth muscles, and serosal layers. Although most PDGFRα-positive ICs labeled CD34, they did not label CD34 in the subepithelial layers. Additionally, PDGFRα-positive ICs were in close proximity to each other, as also to the surrounding cells. TEM and immuno-EM findings revealed that PDGFRα-positive ICs established close physical interactions with adjacent ICs. Extracellular vesicles were also detected around the PDGFRα-positive ICs. Our morphological findings suggest that PDGFRα-positive ICs may have several subpopulations, which can play an important role in intercellular signaling via direct contact with the IC network and the extracellular vesicles in the murine vas deferens. Further investigation on PDGFRα-positive ICs in the vas deferens may lead to understanding the vas deferens mortility.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-021-87049-6</identifier><identifier>PMID: 33824385</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/80/2373 ; 692/4025 ; 692/698 ; CD34 antigen ; Confocal microscopy ; Extracellular vesicles ; Humanities and Social Sciences ; Immunofluorescence ; Interstitial cells ; Lamina propria ; Microscopy ; multidisciplinary ; Muscles ; Platelet-derived growth factor ; Science ; Science (multidisciplinary) ; Subpopulations ; Transmission electron microscopy ; Ultrastructure ; Vas deferens ; Vesicles</subject><ispartof>Scientific reports, 2021-04, Vol.11 (1), p.7553-7553, Article 7553</ispartof><rights>The Author(s) 2021</rights><rights>The Author(s) 2021. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c668t-5f65fcce3c1f5344c5d822629fc7bdeebde6da292685ea92a8c5d5fab2b1c5a83</citedby><cites>FETCH-LOGICAL-c668t-5f65fcce3c1f5344c5d822629fc7bdeebde6da292685ea92a8c5d5fab2b1c5a83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8024294/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8024294/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2095,27903,27904,41099,42168,51555,53770,53772</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33824385$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hiroshige, Tasuku</creatorcontrib><creatorcontrib>Uemura, Kei-Ichiro</creatorcontrib><creatorcontrib>Hirashima, Shingo</creatorcontrib><creatorcontrib>Hino, Kiyosato</creatorcontrib><creatorcontrib>Togo, Akinobu</creatorcontrib><creatorcontrib>Ohta, Keisuke</creatorcontrib><creatorcontrib>Igawa, Tsukasa</creatorcontrib><creatorcontrib>Nakamura, Kei-Ichiro</creatorcontrib><title>Identification of PDGFRα-positive interstitial cells in the distal segment of the murine vas deferens</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Platelet-derived growth factor receptor-α (PDGFRα)-positive interstitial cells (ICs) are widely distributed in various organs and may be involved in the motility of various tubular organs. We, for the first time, aimed to investigate the distribution, immunohistochemical characteristics, and ultrastructure of PDGFRα-positive ICs in murine vas deferens, using confocal laser scanning microscopy, transmission electron microscopy (TEM), and immuno-electron microscopy (immuno-EM). For immunofluorescence, we used antibodies against PDGFRα and other markers of ICs. PDGFRα-positive ICs were distributed widely in the lamina propria, smooth muscles, and serosal layers. Although most PDGFRα-positive ICs labeled CD34, they did not label CD34 in the subepithelial layers. Additionally, PDGFRα-positive ICs were in close proximity to each other, as also to the surrounding cells. TEM and immuno-EM findings revealed that PDGFRα-positive ICs established close physical interactions with adjacent ICs. Extracellular vesicles were also detected around the PDGFRα-positive ICs. Our morphological findings suggest that PDGFRα-positive ICs may have several subpopulations, which can play an important role in intercellular signaling via direct contact with the IC network and the extracellular vesicles in the murine vas deferens. 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We, for the first time, aimed to investigate the distribution, immunohistochemical characteristics, and ultrastructure of PDGFRα-positive ICs in murine vas deferens, using confocal laser scanning microscopy, transmission electron microscopy (TEM), and immuno-electron microscopy (immuno-EM). For immunofluorescence, we used antibodies against PDGFRα and other markers of ICs. PDGFRα-positive ICs were distributed widely in the lamina propria, smooth muscles, and serosal layers. Although most PDGFRα-positive ICs labeled CD34, they did not label CD34 in the subepithelial layers. Additionally, PDGFRα-positive ICs were in close proximity to each other, as also to the surrounding cells. TEM and immuno-EM findings revealed that PDGFRα-positive ICs established close physical interactions with adjacent ICs. Extracellular vesicles were also detected around the PDGFRα-positive ICs. Our morphological findings suggest that PDGFRα-positive ICs may have several subpopulations, which can play an important role in intercellular signaling via direct contact with the IC network and the extracellular vesicles in the murine vas deferens. Further investigation on PDGFRα-positive ICs in the vas deferens may lead to understanding the vas deferens mortility.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>33824385</pmid><doi>10.1038/s41598-021-87049-6</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 631/80/2373 692/4025 692/698 CD34 antigen Confocal microscopy Extracellular vesicles Humanities and Social Sciences Immunofluorescence Interstitial cells Lamina propria Microscopy multidisciplinary Muscles Platelet-derived growth factor Science Science (multidisciplinary) Subpopulations Transmission electron microscopy Ultrastructure Vas deferens Vesicles |
| title | Identification of PDGFRα-positive interstitial cells in the distal segment of the murine vas deferens |
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