Metal Complex Lipids for Fluid–Fluid Phase Separation in Coassembled Phospholipid Membranes
We demonstrate a fluid–fluid phase separation in 1,2‐dimyristoyl‐sn‐glycero‐3‐phosphocholine (DMPC) membranes using a metal complex lipid of type [Mn(L1)] (1; HL1=1‐(2‐hydroxybenzamide)‐2‐(2‐hydroxy‐3‐formyl‐5‐hexadecyloxybenzylideneamino)ethane). Small amount of 1 produces two separated domains in...
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| Veröffentlicht in: | Angewandte Chemie International Edition 2021-06, Vol.60 (24), p.13603-13608 |
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| creator | Ohtani, Ryo Anegawa, Yuka Watanabe, Hikaru Tajima, Yutaro Kinoshita, Masanao Matsumori, Nobuaki Kawano, Kenichi Yanaka, Saeko Kato, Koichi Nakamura, Masaaki Ohba, Masaaki Hayami, Shinya |
| description | We demonstrate a fluid–fluid phase separation in 1,2‐dimyristoyl‐sn‐glycero‐3‐phosphocholine (DMPC) membranes using a metal complex lipid of type [Mn(L1)] (1; HL1=1‐(2‐hydroxybenzamide)‐2‐(2‐hydroxy‐3‐formyl‐5‐hexadecyloxybenzylideneamino)ethane). Small amount of 1 produces two separated domains in DMPC, whose phase transition temperatures of lipids (Tc) are both lower than that of the pristine DMPC. Variable temperature fluorescent microscopy for giant‐unilamellar vesicles of DMPC/1 hybrids demonstrates that visible phase separations remain in fluid phases up to 37 °C, which is clearly over the Tc of DMPC. This provides a new dimension for the application of metal complex lipids toward controlling lipid distributions in fluid membranes.
Coassembly of metal complex lipids with DMPC demonstrates a very rare fluid–fluid phase separation up to 37 °C. |
| doi_str_mv | 10.1002/anie.202102774 |
| format | Article |
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Coassembly of metal complex lipids with DMPC demonstrates a very rare fluid–fluid phase separation up to 37 °C.</description><edition>International ed. in English</edition><identifier>ISSN: 1433-7851</identifier><identifier>EISSN: 1521-3773</identifier><identifier>DOI: 10.1002/anie.202102774</identifier><identifier>PMID: 33723910</identifier><language>eng</language><publisher>Germany: Wiley Subscription Services, Inc</publisher><subject>amphiphile ; Complex lipids ; Coordination compounds ; Ethane ; Fluorescence ; Hybrids ; lipid bilayer ; Lipid rafts ; Lipids ; Membranes ; metal-complex lipid ; Phase separation ; Phase transitions ; Phosphocholine ; Phospholipids ; supramolecules ; Temperature ; Transition temperatures</subject><ispartof>Angewandte Chemie International Edition, 2021-06, Vol.60 (24), p.13603-13608</ispartof><rights>2021 Wiley‐VCH GmbH</rights><rights>2021 Wiley-VCH GmbH.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4764-8727e9f33f46c76236fdd142dcf5c587bfeafe2551af1c85fc29f1159a6abfe73</citedby><cites>FETCH-LOGICAL-c4764-8727e9f33f46c76236fdd142dcf5c587bfeafe2551af1c85fc29f1159a6abfe73</cites><orcidid>0000-0001-8392-2382 ; 0000-0001-9268-3512 ; 0000-0001-7187-9612 ; 0000-0003-0495-2044 ; 0000-0003-1927-2922 ; 0000-0003-4840-3338 ; 0000-0002-3513-5701</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fanie.202102774$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fanie.202102774$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,781,785,1418,27926,27927,45576,45577</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33723910$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ohtani, Ryo</creatorcontrib><creatorcontrib>Anegawa, Yuka</creatorcontrib><creatorcontrib>Watanabe, Hikaru</creatorcontrib><creatorcontrib>Tajima, Yutaro</creatorcontrib><creatorcontrib>Kinoshita, Masanao</creatorcontrib><creatorcontrib>Matsumori, Nobuaki</creatorcontrib><creatorcontrib>Kawano, Kenichi</creatorcontrib><creatorcontrib>Yanaka, Saeko</creatorcontrib><creatorcontrib>Kato, Koichi</creatorcontrib><creatorcontrib>Nakamura, Masaaki</creatorcontrib><creatorcontrib>Ohba, Masaaki</creatorcontrib><creatorcontrib>Hayami, Shinya</creatorcontrib><title>Metal Complex Lipids for Fluid–Fluid Phase Separation in Coassembled Phospholipid Membranes</title><title>Angewandte Chemie International Edition</title><addtitle>Angew Chem Int Ed Engl</addtitle><description>We demonstrate a fluid–fluid phase separation in 1,2‐dimyristoyl‐sn‐glycero‐3‐phosphocholine (DMPC) membranes using a metal complex lipid of type [Mn(L1)] (1; HL1=1‐(2‐hydroxybenzamide)‐2‐(2‐hydroxy‐3‐formyl‐5‐hexadecyloxybenzylideneamino)ethane). Small amount of 1 produces two separated domains in DMPC, whose phase transition temperatures of lipids (Tc) are both lower than that of the pristine DMPC. Variable temperature fluorescent microscopy for giant‐unilamellar vesicles of DMPC/1 hybrids demonstrates that visible phase separations remain in fluid phases up to 37 °C, which is clearly over the Tc of DMPC. This provides a new dimension for the application of metal complex lipids toward controlling lipid distributions in fluid membranes.
Coassembly of metal complex lipids with DMPC demonstrates a very rare fluid–fluid phase separation up to 37 °C.</description><subject>amphiphile</subject><subject>Complex lipids</subject><subject>Coordination compounds</subject><subject>Ethane</subject><subject>Fluorescence</subject><subject>Hybrids</subject><subject>lipid bilayer</subject><subject>Lipid rafts</subject><subject>Lipids</subject><subject>Membranes</subject><subject>metal-complex lipid</subject><subject>Phase separation</subject><subject>Phase transitions</subject><subject>Phosphocholine</subject><subject>Phospholipids</subject><subject>supramolecules</subject><subject>Temperature</subject><subject>Transition temperatures</subject><issn>1433-7851</issn><issn>1521-3773</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNqFkMtKxDAUhoMo3rcupeDGTcdcmqRdyuBlYEYFdSkl055gJG1qMkVn5zv4hj6JGWdUcOPqhJzv_zl8CB0QPCAY0xPVGhhQTAmmUmZraJtwSlImJVuP74yxVOacbKGdEJ4in-dYbKItxiRlBcHb6GECM2WToWs6C6_J2HSmDol2Pjm3vak_3t6_ZnLzqAIkt9Apr2bGtYlpY0iFAM3UwmLvQvfo7CKfTOKnVy2EPbShlQ2wv5q76P787G54mY6vL0bD03FaZVJkaS6phEIzpjNRSUGZ0HVNMlpXmlc8l1MNSgPlnChNqpzrihaaEF4ooeJOsl10vOztvHvuIczKxoQKrI1HuD6UlGMSNcgij-jRH_TJ9b6N10WK8UxkheCRGiypyrsQPOiy86ZRfl4SXC7Elwvx5Y_4GDhc1fbTBuof_Nt0BIol8GIszP-pK0-vRme_5Z-0NpBl</recordid><startdate>20210607</startdate><enddate>20210607</enddate><creator>Ohtani, Ryo</creator><creator>Anegawa, Yuka</creator><creator>Watanabe, Hikaru</creator><creator>Tajima, Yutaro</creator><creator>Kinoshita, Masanao</creator><creator>Matsumori, Nobuaki</creator><creator>Kawano, Kenichi</creator><creator>Yanaka, Saeko</creator><creator>Kato, Koichi</creator><creator>Nakamura, Masaaki</creator><creator>Ohba, Masaaki</creator><creator>Hayami, Shinya</creator><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-8392-2382</orcidid><orcidid>https://orcid.org/0000-0001-9268-3512</orcidid><orcidid>https://orcid.org/0000-0001-7187-9612</orcidid><orcidid>https://orcid.org/0000-0003-0495-2044</orcidid><orcidid>https://orcid.org/0000-0003-1927-2922</orcidid><orcidid>https://orcid.org/0000-0003-4840-3338</orcidid><orcidid>https://orcid.org/0000-0002-3513-5701</orcidid></search><sort><creationdate>20210607</creationdate><title>Metal Complex Lipids for Fluid–Fluid Phase Separation in Coassembled Phospholipid Membranes</title><author>Ohtani, Ryo ; Anegawa, Yuka ; Watanabe, Hikaru ; Tajima, Yutaro ; Kinoshita, Masanao ; Matsumori, Nobuaki ; Kawano, Kenichi ; Yanaka, Saeko ; Kato, Koichi ; Nakamura, Masaaki ; Ohba, Masaaki ; Hayami, Shinya</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4764-8727e9f33f46c76236fdd142dcf5c587bfeafe2551af1c85fc29f1159a6abfe73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>amphiphile</topic><topic>Complex lipids</topic><topic>Coordination compounds</topic><topic>Ethane</topic><topic>Fluorescence</topic><topic>Hybrids</topic><topic>lipid bilayer</topic><topic>Lipid rafts</topic><topic>Lipids</topic><topic>Membranes</topic><topic>metal-complex lipid</topic><topic>Phase separation</topic><topic>Phase transitions</topic><topic>Phosphocholine</topic><topic>Phospholipids</topic><topic>supramolecules</topic><topic>Temperature</topic><topic>Transition temperatures</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ohtani, Ryo</creatorcontrib><creatorcontrib>Anegawa, Yuka</creatorcontrib><creatorcontrib>Watanabe, Hikaru</creatorcontrib><creatorcontrib>Tajima, Yutaro</creatorcontrib><creatorcontrib>Kinoshita, Masanao</creatorcontrib><creatorcontrib>Matsumori, Nobuaki</creatorcontrib><creatorcontrib>Kawano, Kenichi</creatorcontrib><creatorcontrib>Yanaka, Saeko</creatorcontrib><creatorcontrib>Kato, Koichi</creatorcontrib><creatorcontrib>Nakamura, Masaaki</creatorcontrib><creatorcontrib>Ohba, Masaaki</creatorcontrib><creatorcontrib>Hayami, Shinya</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Angewandte Chemie International Edition</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ohtani, Ryo</au><au>Anegawa, Yuka</au><au>Watanabe, Hikaru</au><au>Tajima, Yutaro</au><au>Kinoshita, Masanao</au><au>Matsumori, Nobuaki</au><au>Kawano, Kenichi</au><au>Yanaka, Saeko</au><au>Kato, Koichi</au><au>Nakamura, Masaaki</au><au>Ohba, Masaaki</au><au>Hayami, Shinya</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Metal Complex Lipids for Fluid–Fluid Phase Separation in Coassembled Phospholipid Membranes</atitle><jtitle>Angewandte Chemie International Edition</jtitle><addtitle>Angew Chem Int Ed Engl</addtitle><date>2021-06-07</date><risdate>2021</risdate><volume>60</volume><issue>24</issue><spage>13603</spage><epage>13608</epage><pages>13603-13608</pages><issn>1433-7851</issn><eissn>1521-3773</eissn><abstract>We demonstrate a fluid–fluid phase separation in 1,2‐dimyristoyl‐sn‐glycero‐3‐phosphocholine (DMPC) membranes using a metal complex lipid of type [Mn(L1)] (1; HL1=1‐(2‐hydroxybenzamide)‐2‐(2‐hydroxy‐3‐formyl‐5‐hexadecyloxybenzylideneamino)ethane). Small amount of 1 produces two separated domains in DMPC, whose phase transition temperatures of lipids (Tc) are both lower than that of the pristine DMPC. Variable temperature fluorescent microscopy for giant‐unilamellar vesicles of DMPC/1 hybrids demonstrates that visible phase separations remain in fluid phases up to 37 °C, which is clearly over the Tc of DMPC. This provides a new dimension for the application of metal complex lipids toward controlling lipid distributions in fluid membranes.
Coassembly of metal complex lipids with DMPC demonstrates a very rare fluid–fluid phase separation up to 37 °C.</abstract><cop>Germany</cop><pub>Wiley Subscription Services, Inc</pub><pmid>33723910</pmid><doi>10.1002/anie.202102774</doi><tpages>6</tpages><edition>International ed. in English</edition><orcidid>https://orcid.org/0000-0001-8392-2382</orcidid><orcidid>https://orcid.org/0000-0001-9268-3512</orcidid><orcidid>https://orcid.org/0000-0001-7187-9612</orcidid><orcidid>https://orcid.org/0000-0003-0495-2044</orcidid><orcidid>https://orcid.org/0000-0003-1927-2922</orcidid><orcidid>https://orcid.org/0000-0003-4840-3338</orcidid><orcidid>https://orcid.org/0000-0002-3513-5701</orcidid></addata></record> |
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| source | Wiley-Blackwell Journals |
| subjects | amphiphile Complex lipids Coordination compounds Ethane Fluorescence Hybrids lipid bilayer Lipid rafts Lipids Membranes metal-complex lipid Phase separation Phase transitions Phosphocholine Phospholipids supramolecules Temperature Transition temperatures |
| title | Metal Complex Lipids for Fluid–Fluid Phase Separation in Coassembled Phospholipid Membranes |
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