Influence of Cu 2+ on Osteoclast Formation and Activity In Vitro
Copper-containing biomaterials are increasingly applied for bone regeneration due to their pro-angiogenetic, pro-osteogenetic and antimicrobial properties. Therefore, the effect of Cu on osteoclasts, which play a major role in bone remodeling was studied in detail. Human primary osteoclasts, differe...
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Veröffentlicht in: | International journal of molecular sciences 2021-02, Vol.22 (5) |
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creator | Bernhardt, Anne Bacova, Jana Gbureck, Uwe Gelinsky, Michael |
description | Copper-containing biomaterials are increasingly applied for bone regeneration due to their pro-angiogenetic, pro-osteogenetic and antimicrobial properties. Therefore, the effect of Cu
on osteoclasts, which play a major role in bone remodeling was studied in detail.
Human primary osteoclasts, differentiated from human monocytes were differentiated or cultivated in the presence of Cu
. Osteoclast formation and activity were analyzed by measurement of osteoclast-specific enzyme activities, gene expression analysis and resorption assays. Furthermore, the glutathione levels of the cells were checked to evaluate oxidative stress induced by Cu
.
Up to 8 µM Cu
did not induce cytotoxic effects. Activity of tartrate-resistant acid phosphatase (TRAP) was significantly increased, while other osteoclast specific enzyme activities were not affected. However, gene expression of TRAP was not upregulated. Resorptive activity of osteoclasts towards dentin was not changed in the presence of 8 µM Cu
but decreased in the presence of extracellular bone matrix. When Cu
was added to mature osteoclasts TRAP activity was not increased and resorption decreased only moderately. The glutathione level of both differentiating and mature osteoclasts was significantly decreased in the presence of Cu
.
Differentiating and mature osteoclasts react differently to Cu
. High TRAP activities are not necessarily related to high resorption. |
format | Article |
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on osteoclasts, which play a major role in bone remodeling was studied in detail.
Human primary osteoclasts, differentiated from human monocytes were differentiated or cultivated in the presence of Cu
. Osteoclast formation and activity were analyzed by measurement of osteoclast-specific enzyme activities, gene expression analysis and resorption assays. Furthermore, the glutathione levels of the cells were checked to evaluate oxidative stress induced by Cu
.
Up to 8 µM Cu
did not induce cytotoxic effects. Activity of tartrate-resistant acid phosphatase (TRAP) was significantly increased, while other osteoclast specific enzyme activities were not affected. However, gene expression of TRAP was not upregulated. Resorptive activity of osteoclasts towards dentin was not changed in the presence of 8 µM Cu
but decreased in the presence of extracellular bone matrix. When Cu
was added to mature osteoclasts TRAP activity was not increased and resorption decreased only moderately. The glutathione level of both differentiating and mature osteoclasts was significantly decreased in the presence of Cu
.
Differentiating and mature osteoclasts react differently to Cu
. High TRAP activities are not necessarily related to high resorption.</description><identifier>EISSN: 1422-0067</identifier><identifier>PMID: 33671069</identifier><language>eng</language><publisher>Switzerland</publisher><subject>Animals ; Bone Remodeling - drug effects ; Bone Resorption ; Cell Differentiation ; Cells, Cultured ; Copper - pharmacology ; Dentin - metabolism ; Gene Expression Regulation ; Humans ; In Vitro Techniques ; Leukocytes, Mononuclear - cytology ; Leukocytes, Mononuclear - drug effects ; Leukocytes, Mononuclear - metabolism ; Osteoclasts - cytology ; Osteoclasts - drug effects ; Osteoclasts - metabolism ; Swine ; Tartrate-Resistant Acid Phosphatase - metabolism</subject><ispartof>International journal of molecular sciences, 2021-02, Vol.22 (5)</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><orcidid>0000-0001-9075-5121 ; 0000-0002-1903-1929</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33671069$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bernhardt, Anne</creatorcontrib><creatorcontrib>Bacova, Jana</creatorcontrib><creatorcontrib>Gbureck, Uwe</creatorcontrib><creatorcontrib>Gelinsky, Michael</creatorcontrib><title>Influence of Cu 2+ on Osteoclast Formation and Activity In Vitro</title><title>International journal of molecular sciences</title><addtitle>Int J Mol Sci</addtitle><description>Copper-containing biomaterials are increasingly applied for bone regeneration due to their pro-angiogenetic, pro-osteogenetic and antimicrobial properties. Therefore, the effect of Cu
on osteoclasts, which play a major role in bone remodeling was studied in detail.
Human primary osteoclasts, differentiated from human monocytes were differentiated or cultivated in the presence of Cu
. Osteoclast formation and activity were analyzed by measurement of osteoclast-specific enzyme activities, gene expression analysis and resorption assays. Furthermore, the glutathione levels of the cells were checked to evaluate oxidative stress induced by Cu
.
Up to 8 µM Cu
did not induce cytotoxic effects. Activity of tartrate-resistant acid phosphatase (TRAP) was significantly increased, while other osteoclast specific enzyme activities were not affected. However, gene expression of TRAP was not upregulated. Resorptive activity of osteoclasts towards dentin was not changed in the presence of 8 µM Cu
but decreased in the presence of extracellular bone matrix. When Cu
was added to mature osteoclasts TRAP activity was not increased and resorption decreased only moderately. The glutathione level of both differentiating and mature osteoclasts was significantly decreased in the presence of Cu
.
Differentiating and mature osteoclasts react differently to Cu
. High TRAP activities are not necessarily related to high resorption.</description><subject>Animals</subject><subject>Bone Remodeling - drug effects</subject><subject>Bone Resorption</subject><subject>Cell Differentiation</subject><subject>Cells, Cultured</subject><subject>Copper - pharmacology</subject><subject>Dentin - metabolism</subject><subject>Gene Expression Regulation</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Leukocytes, Mononuclear - cytology</subject><subject>Leukocytes, Mononuclear - drug effects</subject><subject>Leukocytes, Mononuclear - metabolism</subject><subject>Osteoclasts - cytology</subject><subject>Osteoclasts - drug effects</subject><subject>Osteoclasts - metabolism</subject><subject>Swine</subject><subject>Tartrate-Resistant Acid Phosphatase - metabolism</subject><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpjYuA0NDEy0jUwMDPnYOAqLs4yMDAyNjK1ZGfgMDY2Mzc0MLPkZHDwzEvLKU3NS05VyE9TcC5VMNJWyM9T8C8uSc1PzkksLlFwyy_KTSzJBAom5qUoOCaXZJZlllQqeOYphGWWFOXzMLCmJeYUp_JCaW4GOTfXEGcP3YLSpNzUlPiCoszcxKLKeJiVxgQVAAAfkDUv</recordid><startdate>20210228</startdate><enddate>20210228</enddate><creator>Bernhardt, Anne</creator><creator>Bacova, Jana</creator><creator>Gbureck, Uwe</creator><creator>Gelinsky, Michael</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><orcidid>https://orcid.org/0000-0001-9075-5121</orcidid><orcidid>https://orcid.org/0000-0002-1903-1929</orcidid></search><sort><creationdate>20210228</creationdate><title>Influence of Cu 2+ on Osteoclast Formation and Activity In Vitro</title><author>Bernhardt, Anne ; Bacova, Jana ; Gbureck, Uwe ; Gelinsky, Michael</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-pubmed_primary_336710693</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Animals</topic><topic>Bone Remodeling - drug effects</topic><topic>Bone Resorption</topic><topic>Cell Differentiation</topic><topic>Cells, Cultured</topic><topic>Copper - pharmacology</topic><topic>Dentin - metabolism</topic><topic>Gene Expression Regulation</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>Leukocytes, Mononuclear - cytology</topic><topic>Leukocytes, Mononuclear - drug effects</topic><topic>Leukocytes, Mononuclear - metabolism</topic><topic>Osteoclasts - cytology</topic><topic>Osteoclasts - drug effects</topic><topic>Osteoclasts - metabolism</topic><topic>Swine</topic><topic>Tartrate-Resistant Acid Phosphatase - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bernhardt, Anne</creatorcontrib><creatorcontrib>Bacova, Jana</creatorcontrib><creatorcontrib>Gbureck, Uwe</creatorcontrib><creatorcontrib>Gelinsky, Michael</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>International journal of molecular sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bernhardt, Anne</au><au>Bacova, Jana</au><au>Gbureck, Uwe</au><au>Gelinsky, Michael</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Influence of Cu 2+ on Osteoclast Formation and Activity In Vitro</atitle><jtitle>International journal of molecular sciences</jtitle><addtitle>Int J Mol Sci</addtitle><date>2021-02-28</date><risdate>2021</risdate><volume>22</volume><issue>5</issue><eissn>1422-0067</eissn><abstract>Copper-containing biomaterials are increasingly applied for bone regeneration due to their pro-angiogenetic, pro-osteogenetic and antimicrobial properties. Therefore, the effect of Cu
on osteoclasts, which play a major role in bone remodeling was studied in detail.
Human primary osteoclasts, differentiated from human monocytes were differentiated or cultivated in the presence of Cu
. Osteoclast formation and activity were analyzed by measurement of osteoclast-specific enzyme activities, gene expression analysis and resorption assays. Furthermore, the glutathione levels of the cells were checked to evaluate oxidative stress induced by Cu
.
Up to 8 µM Cu
did not induce cytotoxic effects. Activity of tartrate-resistant acid phosphatase (TRAP) was significantly increased, while other osteoclast specific enzyme activities were not affected. However, gene expression of TRAP was not upregulated. Resorptive activity of osteoclasts towards dentin was not changed in the presence of 8 µM Cu
but decreased in the presence of extracellular bone matrix. When Cu
was added to mature osteoclasts TRAP activity was not increased and resorption decreased only moderately. The glutathione level of both differentiating and mature osteoclasts was significantly decreased in the presence of Cu
.
Differentiating and mature osteoclasts react differently to Cu
. High TRAP activities are not necessarily related to high resorption.</abstract><cop>Switzerland</cop><pmid>33671069</pmid><orcidid>https://orcid.org/0000-0001-9075-5121</orcidid><orcidid>https://orcid.org/0000-0002-1903-1929</orcidid></addata></record> |
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source | MEDLINE; MDPI - Multidisciplinary Digital Publishing Institute; EZB-FREE-00999 freely available EZB journals; PubMed Central |
subjects | Animals Bone Remodeling - drug effects Bone Resorption Cell Differentiation Cells, Cultured Copper - pharmacology Dentin - metabolism Gene Expression Regulation Humans In Vitro Techniques Leukocytes, Mononuclear - cytology Leukocytes, Mononuclear - drug effects Leukocytes, Mononuclear - metabolism Osteoclasts - cytology Osteoclasts - drug effects Osteoclasts - metabolism Swine Tartrate-Resistant Acid Phosphatase - metabolism |
title | Influence of Cu 2+ on Osteoclast Formation and Activity In Vitro |
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