Bacillus Toyonensis BCT-7112 T Spores as Parenteral Adjuvant of BoHV-5 Vaccine in a Murine Model
Bacterial spores of the genus Bacillus are being evaluated as adjuvant molecules capable of improving the immune response to vaccines. In this study, we investigate whether subcutaneously administered spores of B. toyonensis BCT-7112 could enhance a vaccine immune response in mice. Three groups of m...
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| Veröffentlicht in: | Probiotics and antimicrobial proteins 2021-06, Vol.13 (3), p.655 |
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| creator | Santos, Francisco Denis Souza Maubrigades, Lucas Reichert Gonçalves, Vitória Sequeira Franz, Helen Cabaldi Rodrigues, Paulo Ricardo Centeno Cunha, Rodrigo Casquero Leite, Fábio Pereira Leivas |
| description | Bacterial spores of the genus Bacillus are being evaluated as adjuvant molecules capable of improving the immune response to vaccines. In this study, we investigate whether subcutaneously administered spores of B. toyonensis BCT-7112
could enhance a vaccine immune response in mice. Three groups of mice were subcutaneously vaccinated on day 0 and received a booster on day 21 of the experiment, with the following vaccine formulations: 40 µg of recombinant glycoprotein D (rgD) from bovine herpesvirus type 5 (BoHV-5) adsorbed in 10% aluminum hydroxide (alum) without B. toyonensis spores (group 1) and B. toyonensis (1 × 10
viable spores) + 40 µg of rgD adsorbed in 10% alum (group 2); and B. toyonensis (1 × 10
viable spores) without rgD (group 3). Group 2 showed significantly higher titers (P |
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could enhance a vaccine immune response in mice. Three groups of mice were subcutaneously vaccinated on day 0 and received a booster on day 21 of the experiment, with the following vaccine formulations: 40 µg of recombinant glycoprotein D (rgD) from bovine herpesvirus type 5 (BoHV-5) adsorbed in 10% aluminum hydroxide (alum) without B. toyonensis spores (group 1) and B. toyonensis (1 × 10
viable spores) + 40 µg of rgD adsorbed in 10% alum (group 2); and B. toyonensis (1 × 10
viable spores) without rgD (group 3). Group 2 showed significantly higher titers (P < 0.05) of total specific serum IgG, IgG2a, and neutralizing antibodies, when compared with the groups 1 and 3. A significantly higher (P < 0.05) transcription level of cytokines IL-4, IL-12, and IFN-γ was observed in splenocytes from mice that received the B. toyonensis spores in the vaccine formulation. In addition, stimulation of the macrophage-like cell line RAW264.7 with spores of B. toyonensis markedly enhanced the cell proliferation and mRNA transcription levels of IL-4, and IL-12 cytokines in these cells. Our findings indicated that the subcutaneous administration of B. toyonensis BCT-7112
spores enhanced the humoral and cellular immune response against BoHV-5 in mice.</description><identifier>EISSN: 1867-1314</identifier><identifier>PMID: 33608827</identifier><language>eng</language><publisher>United States</publisher><subject>Adjuvants, Immunologic ; Animals ; Bacillus - immunology ; Disease Models, Animal ; Herpesviridae Infections - prevention & control ; Herpesvirus 5, Bovine ; Interleukin-12 ; Interleukin-4 ; Mice ; Oligopeptides ; Spores, Bacterial - immunology ; Viral Vaccines - immunology</subject><ispartof>Probiotics and antimicrobial proteins, 2021-06, Vol.13 (3), p.655</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><orcidid>0000-0003-0941-7286</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33608827$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Santos, Francisco Denis Souza</creatorcontrib><creatorcontrib>Maubrigades, Lucas Reichert</creatorcontrib><creatorcontrib>Gonçalves, Vitória Sequeira</creatorcontrib><creatorcontrib>Franz, Helen Cabaldi</creatorcontrib><creatorcontrib>Rodrigues, Paulo Ricardo Centeno</creatorcontrib><creatorcontrib>Cunha, Rodrigo Casquero</creatorcontrib><creatorcontrib>Leite, Fábio Pereira Leivas</creatorcontrib><title>Bacillus Toyonensis BCT-7112 T Spores as Parenteral Adjuvant of BoHV-5 Vaccine in a Murine Model</title><title>Probiotics and antimicrobial proteins</title><addtitle>Probiotics Antimicrob Proteins</addtitle><description>Bacterial spores of the genus Bacillus are being evaluated as adjuvant molecules capable of improving the immune response to vaccines. In this study, we investigate whether subcutaneously administered spores of B. toyonensis BCT-7112
could enhance a vaccine immune response in mice. Three groups of mice were subcutaneously vaccinated on day 0 and received a booster on day 21 of the experiment, with the following vaccine formulations: 40 µg of recombinant glycoprotein D (rgD) from bovine herpesvirus type 5 (BoHV-5) adsorbed in 10% aluminum hydroxide (alum) without B. toyonensis spores (group 1) and B. toyonensis (1 × 10
viable spores) + 40 µg of rgD adsorbed in 10% alum (group 2); and B. toyonensis (1 × 10
viable spores) without rgD (group 3). Group 2 showed significantly higher titers (P < 0.05) of total specific serum IgG, IgG2a, and neutralizing antibodies, when compared with the groups 1 and 3. A significantly higher (P < 0.05) transcription level of cytokines IL-4, IL-12, and IFN-γ was observed in splenocytes from mice that received the B. toyonensis spores in the vaccine formulation. In addition, stimulation of the macrophage-like cell line RAW264.7 with spores of B. toyonensis markedly enhanced the cell proliferation and mRNA transcription levels of IL-4, and IL-12 cytokines in these cells. Our findings indicated that the subcutaneous administration of B. toyonensis BCT-7112
spores enhanced the humoral and cellular immune response against BoHV-5 in mice.</description><subject>Adjuvants, Immunologic</subject><subject>Animals</subject><subject>Bacillus - immunology</subject><subject>Disease Models, Animal</subject><subject>Herpesviridae Infections - prevention & control</subject><subject>Herpesvirus 5, Bovine</subject><subject>Interleukin-12</subject><subject>Interleukin-4</subject><subject>Mice</subject><subject>Oligopeptides</subject><subject>Spores, Bacterial - immunology</subject><subject>Viral Vaccines - immunology</subject><issn>1867-1314</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFjk8LgjAcQEcQaX--Qvy-gOBcqdeUwksQNLzaL50wmZtsGvjtI6hzp_cO7_AWxKdpnASU0YNH1s51YRjHLApXxGMsDtM0SnzyyLCWSk0OuJmNFtpJB1nOg4TSCDjcB2OFA3RwQyv0KCwqODXd9EI9gmkhM0UZHKHEupZagNSAcJ3sx6-mEWpLli0qJ3Zfbsj-cuZ5EQzTsxdNNVjZo52r3xL7G7wBq3I_YQ</recordid><startdate>202106</startdate><enddate>202106</enddate><creator>Santos, Francisco Denis Souza</creator><creator>Maubrigades, Lucas Reichert</creator><creator>Gonçalves, Vitória Sequeira</creator><creator>Franz, Helen Cabaldi</creator><creator>Rodrigues, Paulo Ricardo Centeno</creator><creator>Cunha, Rodrigo Casquero</creator><creator>Leite, Fábio Pereira Leivas</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><orcidid>https://orcid.org/0000-0003-0941-7286</orcidid></search><sort><creationdate>202106</creationdate><title>Bacillus Toyonensis BCT-7112 T Spores as Parenteral Adjuvant of BoHV-5 Vaccine in a Murine Model</title><author>Santos, Francisco Denis Souza ; Maubrigades, Lucas Reichert ; Gonçalves, Vitória Sequeira ; Franz, Helen Cabaldi ; Rodrigues, Paulo Ricardo Centeno ; Cunha, Rodrigo Casquero ; Leite, Fábio Pereira Leivas</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-pubmed_primary_336088273</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Adjuvants, Immunologic</topic><topic>Animals</topic><topic>Bacillus - immunology</topic><topic>Disease Models, Animal</topic><topic>Herpesviridae Infections - prevention & control</topic><topic>Herpesvirus 5, Bovine</topic><topic>Interleukin-12</topic><topic>Interleukin-4</topic><topic>Mice</topic><topic>Oligopeptides</topic><topic>Spores, Bacterial - immunology</topic><topic>Viral Vaccines - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Santos, Francisco Denis Souza</creatorcontrib><creatorcontrib>Maubrigades, Lucas Reichert</creatorcontrib><creatorcontrib>Gonçalves, Vitória Sequeira</creatorcontrib><creatorcontrib>Franz, Helen Cabaldi</creatorcontrib><creatorcontrib>Rodrigues, Paulo Ricardo Centeno</creatorcontrib><creatorcontrib>Cunha, Rodrigo Casquero</creatorcontrib><creatorcontrib>Leite, Fábio Pereira Leivas</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>Probiotics and antimicrobial proteins</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Santos, Francisco Denis Souza</au><au>Maubrigades, Lucas Reichert</au><au>Gonçalves, Vitória Sequeira</au><au>Franz, Helen Cabaldi</au><au>Rodrigues, Paulo Ricardo Centeno</au><au>Cunha, Rodrigo Casquero</au><au>Leite, Fábio Pereira Leivas</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bacillus Toyonensis BCT-7112 T Spores as Parenteral Adjuvant of BoHV-5 Vaccine in a Murine Model</atitle><jtitle>Probiotics and antimicrobial proteins</jtitle><addtitle>Probiotics Antimicrob Proteins</addtitle><date>2021-06</date><risdate>2021</risdate><volume>13</volume><issue>3</issue><spage>655</spage><pages>655-</pages><eissn>1867-1314</eissn><abstract>Bacterial spores of the genus Bacillus are being evaluated as adjuvant molecules capable of improving the immune response to vaccines. In this study, we investigate whether subcutaneously administered spores of B. toyonensis BCT-7112
could enhance a vaccine immune response in mice. Three groups of mice were subcutaneously vaccinated on day 0 and received a booster on day 21 of the experiment, with the following vaccine formulations: 40 µg of recombinant glycoprotein D (rgD) from bovine herpesvirus type 5 (BoHV-5) adsorbed in 10% aluminum hydroxide (alum) without B. toyonensis spores (group 1) and B. toyonensis (1 × 10
viable spores) + 40 µg of rgD adsorbed in 10% alum (group 2); and B. toyonensis (1 × 10
viable spores) without rgD (group 3). Group 2 showed significantly higher titers (P < 0.05) of total specific serum IgG, IgG2a, and neutralizing antibodies, when compared with the groups 1 and 3. A significantly higher (P < 0.05) transcription level of cytokines IL-4, IL-12, and IFN-γ was observed in splenocytes from mice that received the B. toyonensis spores in the vaccine formulation. In addition, stimulation of the macrophage-like cell line RAW264.7 with spores of B. toyonensis markedly enhanced the cell proliferation and mRNA transcription levels of IL-4, and IL-12 cytokines in these cells. Our findings indicated that the subcutaneous administration of B. toyonensis BCT-7112
spores enhanced the humoral and cellular immune response against BoHV-5 in mice.</abstract><cop>United States</cop><pmid>33608827</pmid><orcidid>https://orcid.org/0000-0003-0941-7286</orcidid></addata></record> |
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| ispartof | Probiotics and antimicrobial proteins, 2021-06, Vol.13 (3), p.655 |
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| source | MEDLINE; SpringerNature Journals |
| subjects | Adjuvants, Immunologic Animals Bacillus - immunology Disease Models, Animal Herpesviridae Infections - prevention & control Herpesvirus 5, Bovine Interleukin-12 Interleukin-4 Mice Oligopeptides Spores, Bacterial - immunology Viral Vaccines - immunology |
| title | Bacillus Toyonensis BCT-7112 T Spores as Parenteral Adjuvant of BoHV-5 Vaccine in a Murine Model |
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