Epitope recognition of magnetic peptide-imprinted chitosan composite nanoparticles for the extraction of CRISPR/dCas9a proteins from transfected cells

The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas9) technology is a powerful method for genetic modification (and regulation) that is of great current interest. The development of new, economical methods of detecting and extracting Cas9 (and/or dCa...

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Veröffentlicht in:	Nanotechnology 2021-04, Vol.32 (18), p.18LT02-18LT02
Hauptverfasser:	Lee, Mei-Hwa, Lin, Cheng-Chih, Thomas, James L, Chan, Chih-Kai, Lin, Hung-Yin
Format:	Artikel
Sprache:	eng
Schlagworte:	Chitosan - chemistry Clustered Regularly Interspaced Short Palindromic Repeats CRISPR-Associated Protein 9 - isolation & purification CRISPR-Cas Systems CRISPR/dCas9a proteins Epitopes - isolation & purification extraction Gene Editing HEK293 Cells Humans magnetic nanoparticles Magnets - chemistry Molecular Imprinting Nanoparticles - chemistry peptide-imprinting Peptides - chemistry Transfection
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