Eugenol loaded chitosan nanoemulsion for food protection and inhibition of Aflatoxin B 1 synthesizing genes based on molecular docking
The present investigation entails the fabrication and characterization of nanometric emulsion of eugenol (Nm-eugenol) encompassed into chitosan for assessing bio-efficacy in terms of in vitro antifungal actions, antiaflatoxigenic potential, and in situ preservative efficacy against Aspergillus flavu...
Gespeichert in:
| Veröffentlicht in: | Carbohydrate polymers 2021-03, Vol.255, p.117339 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | 117339 |
| container_title | Carbohydrate polymers |
| container_volume | 255 |
| creator | Das, Somenath Singh, Vipin Kumar Dwivedy, Abhishek Kumar Chaudhari, Anand Kumar Deepika Dubey, Nawal Kishore |
| description | The present investigation entails the fabrication and characterization of nanometric emulsion of eugenol (Nm-eugenol) encompassed into chitosan for assessing bio-efficacy in terms of in vitro antifungal actions, antiaflatoxigenic potential, and in situ preservative efficacy against Aspergillus flavus infestation and aflatoxin B
(AFB
) mediated loss of dietary minerals, lipid triglycerides and alterations in composition of important macronutrients in stored rice. Nm-eugenol characterized by SEM, XRD, and FTIR exhibited biphasic burst release of eugenol. Reduction in ergosterol and methylglyoxal (AFB
-inducer) content after Nm-eugenol fumigation depicted biochemical mechanism of antifungal and antiaflatoxigenic activities. In silico 3D homology docking of eugenol with Ver-1 gene validated molecular mechanism of AFB
inhibition. Further, significant protection of rice seeds from fungi, AFB
contamination and preservation against loss of rice minerals, macronutrients and lipids during storage suggested deployment of chitosan as a biocompatible wall material for eugenol encapsulation and application as novel green preservative for food protection. |
| doi_str_mv | 10.1016/j.carbpol.2020.117339 |
| format | Article |
| fullrecord | <record><control><sourceid>pubmed</sourceid><recordid>TN_cdi_pubmed_primary_33436182</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>33436182</sourcerecordid><originalsourceid>FETCH-LOGICAL-p108t-6081fd66ddf0df83360927a9ab42b8142377e822f935b90917a8356ac55752c23</originalsourceid><addsrcrecordid>eNo1kOlKAzEUhYMgti6PoOQFpmaZyfKzlrpAwT_6uySTpE3NJMNkBqwP4HMbtwuHy_k43AMXgGuMFhhhdntYtGrQfQoLgkhhmFMqT8AcCy4rTOt6Bs5zPqAyDKMzMKO0pgwLMgef62lnYwowJGWsge3ejymrCKOKyXZTyD5F6NJQlAzshzTadvxmKhro495r_2OTg0sX1JjefYR3EMN8jOPeZv_h4w6WDpuhVrlUlHCXgm2noAZoUvtWApfg1KmQ7dXfvgCv9-uX1WO1eX54Wi03VY-RGCuGBHaGMWMcMk5QypAkXEmla6IFrgnl3ApCnKSNlkhirgRtmGqbhjekJfQC3Pze7SfdWbPtB9-p4bj9fwj9AvK6ZO8</addsrcrecordid><sourcetype>Index Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Eugenol loaded chitosan nanoemulsion for food protection and inhibition of Aflatoxin B 1 synthesizing genes based on molecular docking</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Das, Somenath ; Singh, Vipin Kumar ; Dwivedy, Abhishek Kumar ; Chaudhari, Anand Kumar ; Deepika ; Dubey, Nawal Kishore</creator><creatorcontrib>Das, Somenath ; Singh, Vipin Kumar ; Dwivedy, Abhishek Kumar ; Chaudhari, Anand Kumar ; Deepika ; Dubey, Nawal Kishore</creatorcontrib><description>The present investigation entails the fabrication and characterization of nanometric emulsion of eugenol (Nm-eugenol) encompassed into chitosan for assessing bio-efficacy in terms of in vitro antifungal actions, antiaflatoxigenic potential, and in situ preservative efficacy against Aspergillus flavus infestation and aflatoxin B
(AFB
) mediated loss of dietary minerals, lipid triglycerides and alterations in composition of important macronutrients in stored rice. Nm-eugenol characterized by SEM, XRD, and FTIR exhibited biphasic burst release of eugenol. Reduction in ergosterol and methylglyoxal (AFB
-inducer) content after Nm-eugenol fumigation depicted biochemical mechanism of antifungal and antiaflatoxigenic activities. In silico 3D homology docking of eugenol with Ver-1 gene validated molecular mechanism of AFB
inhibition. Further, significant protection of rice seeds from fungi, AFB
contamination and preservation against loss of rice minerals, macronutrients and lipids during storage suggested deployment of chitosan as a biocompatible wall material for eugenol encapsulation and application as novel green preservative for food protection.</description><identifier>EISSN: 1879-1344</identifier><identifier>DOI: 10.1016/j.carbpol.2020.117339</identifier><identifier>PMID: 33436182</identifier><language>eng</language><publisher>England</publisher><subject>Aflatoxin B1 - antagonists & inhibitors ; Aflatoxin B1 - chemistry ; Aflatoxin B1 - metabolism ; Aflatoxin B1 - toxicity ; Anti-Infective Agents - metabolism ; Anti-Infective Agents - pharmacology ; Aspergillus flavus - drug effects ; Aspergillus flavus - growth & development ; Aspergillus flavus - metabolism ; Binding Sites ; Chitosan - chemistry ; Drug Compounding - methods ; Drug Liberation ; Edible Grain ; Emulsions ; Ergosterol - antagonists & inhibitors ; Ergosterol - metabolism ; Eugenol - metabolism ; Eugenol - pharmacology ; Food Preservation - methods ; Fungal Proteins - antagonists & inhibitors ; Fungal Proteins - chemistry ; Fungal Proteins - metabolism ; Humans ; Kinetics ; Molecular Docking Simulation ; Nanostructures - chemistry ; Nanostructures - ultrastructure ; Nutrients - analysis ; Oryza - drug effects ; Oryza - microbiology ; Protein Binding ; Protein Conformation, alpha-Helical ; Protein Conformation, beta-Strand ; Pyruvaldehyde - antagonists & inhibitors ; Pyruvaldehyde - metabolism ; Triglycerides - analysis</subject><ispartof>Carbohydrate polymers, 2021-03, Vol.255, p.117339</ispartof><rights>Copyright © 2020 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33436182$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Das, Somenath</creatorcontrib><creatorcontrib>Singh, Vipin Kumar</creatorcontrib><creatorcontrib>Dwivedy, Abhishek Kumar</creatorcontrib><creatorcontrib>Chaudhari, Anand Kumar</creatorcontrib><creatorcontrib>Deepika</creatorcontrib><creatorcontrib>Dubey, Nawal Kishore</creatorcontrib><title>Eugenol loaded chitosan nanoemulsion for food protection and inhibition of Aflatoxin B 1 synthesizing genes based on molecular docking</title><title>Carbohydrate polymers</title><addtitle>Carbohydr Polym</addtitle><description>The present investigation entails the fabrication and characterization of nanometric emulsion of eugenol (Nm-eugenol) encompassed into chitosan for assessing bio-efficacy in terms of in vitro antifungal actions, antiaflatoxigenic potential, and in situ preservative efficacy against Aspergillus flavus infestation and aflatoxin B
(AFB
) mediated loss of dietary minerals, lipid triglycerides and alterations in composition of important macronutrients in stored rice. Nm-eugenol characterized by SEM, XRD, and FTIR exhibited biphasic burst release of eugenol. Reduction in ergosterol and methylglyoxal (AFB
-inducer) content after Nm-eugenol fumigation depicted biochemical mechanism of antifungal and antiaflatoxigenic activities. In silico 3D homology docking of eugenol with Ver-1 gene validated molecular mechanism of AFB
inhibition. Further, significant protection of rice seeds from fungi, AFB
contamination and preservation against loss of rice minerals, macronutrients and lipids during storage suggested deployment of chitosan as a biocompatible wall material for eugenol encapsulation and application as novel green preservative for food protection.</description><subject>Aflatoxin B1 - antagonists & inhibitors</subject><subject>Aflatoxin B1 - chemistry</subject><subject>Aflatoxin B1 - metabolism</subject><subject>Aflatoxin B1 - toxicity</subject><subject>Anti-Infective Agents - metabolism</subject><subject>Anti-Infective Agents - pharmacology</subject><subject>Aspergillus flavus - drug effects</subject><subject>Aspergillus flavus - growth & development</subject><subject>Aspergillus flavus - metabolism</subject><subject>Binding Sites</subject><subject>Chitosan - chemistry</subject><subject>Drug Compounding - methods</subject><subject>Drug Liberation</subject><subject>Edible Grain</subject><subject>Emulsions</subject><subject>Ergosterol - antagonists & inhibitors</subject><subject>Ergosterol - metabolism</subject><subject>Eugenol - metabolism</subject><subject>Eugenol - pharmacology</subject><subject>Food Preservation - methods</subject><subject>Fungal Proteins - antagonists & inhibitors</subject><subject>Fungal Proteins - chemistry</subject><subject>Fungal Proteins - metabolism</subject><subject>Humans</subject><subject>Kinetics</subject><subject>Molecular Docking Simulation</subject><subject>Nanostructures - chemistry</subject><subject>Nanostructures - ultrastructure</subject><subject>Nutrients - analysis</subject><subject>Oryza - drug effects</subject><subject>Oryza - microbiology</subject><subject>Protein Binding</subject><subject>Protein Conformation, alpha-Helical</subject><subject>Protein Conformation, beta-Strand</subject><subject>Pyruvaldehyde - antagonists & inhibitors</subject><subject>Pyruvaldehyde - metabolism</subject><subject>Triglycerides - analysis</subject><issn>1879-1344</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kOlKAzEUhYMgti6PoOQFpmaZyfKzlrpAwT_6uySTpE3NJMNkBqwP4HMbtwuHy_k43AMXgGuMFhhhdntYtGrQfQoLgkhhmFMqT8AcCy4rTOt6Bs5zPqAyDKMzMKO0pgwLMgef62lnYwowJGWsge3ejymrCKOKyXZTyD5F6NJQlAzshzTadvxmKhro495r_2OTg0sX1JjefYR3EMN8jOPeZv_h4w6WDpuhVrlUlHCXgm2noAZoUvtWApfg1KmQ7dXfvgCv9-uX1WO1eX54Wi03VY-RGCuGBHaGMWMcMk5QypAkXEmla6IFrgnl3ApCnKSNlkhirgRtmGqbhjekJfQC3Pze7SfdWbPtB9-p4bj9fwj9AvK6ZO8</recordid><startdate>20210301</startdate><enddate>20210301</enddate><creator>Das, Somenath</creator><creator>Singh, Vipin Kumar</creator><creator>Dwivedy, Abhishek Kumar</creator><creator>Chaudhari, Anand Kumar</creator><creator>Deepika</creator><creator>Dubey, Nawal Kishore</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>20210301</creationdate><title>Eugenol loaded chitosan nanoemulsion for food protection and inhibition of Aflatoxin B 1 synthesizing genes based on molecular docking</title><author>Das, Somenath ; Singh, Vipin Kumar ; Dwivedy, Abhishek Kumar ; Chaudhari, Anand Kumar ; Deepika ; Dubey, Nawal Kishore</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p108t-6081fd66ddf0df83360927a9ab42b8142377e822f935b90917a8356ac55752c23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Aflatoxin B1 - antagonists & inhibitors</topic><topic>Aflatoxin B1 - chemistry</topic><topic>Aflatoxin B1 - metabolism</topic><topic>Aflatoxin B1 - toxicity</topic><topic>Anti-Infective Agents - metabolism</topic><topic>Anti-Infective Agents - pharmacology</topic><topic>Aspergillus flavus - drug effects</topic><topic>Aspergillus flavus - growth & development</topic><topic>Aspergillus flavus - metabolism</topic><topic>Binding Sites</topic><topic>Chitosan - chemistry</topic><topic>Drug Compounding - methods</topic><topic>Drug Liberation</topic><topic>Edible Grain</topic><topic>Emulsions</topic><topic>Ergosterol - antagonists & inhibitors</topic><topic>Ergosterol - metabolism</topic><topic>Eugenol - metabolism</topic><topic>Eugenol - pharmacology</topic><topic>Food Preservation - methods</topic><topic>Fungal Proteins - antagonists & inhibitors</topic><topic>Fungal Proteins - chemistry</topic><topic>Fungal Proteins - metabolism</topic><topic>Humans</topic><topic>Kinetics</topic><topic>Molecular Docking Simulation</topic><topic>Nanostructures - chemistry</topic><topic>Nanostructures - ultrastructure</topic><topic>Nutrients - analysis</topic><topic>Oryza - drug effects</topic><topic>Oryza - microbiology</topic><topic>Protein Binding</topic><topic>Protein Conformation, alpha-Helical</topic><topic>Protein Conformation, beta-Strand</topic><topic>Pyruvaldehyde - antagonists & inhibitors</topic><topic>Pyruvaldehyde - metabolism</topic><topic>Triglycerides - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Das, Somenath</creatorcontrib><creatorcontrib>Singh, Vipin Kumar</creatorcontrib><creatorcontrib>Dwivedy, Abhishek Kumar</creatorcontrib><creatorcontrib>Chaudhari, Anand Kumar</creatorcontrib><creatorcontrib>Deepika</creatorcontrib><creatorcontrib>Dubey, Nawal Kishore</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>Carbohydrate polymers</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Das, Somenath</au><au>Singh, Vipin Kumar</au><au>Dwivedy, Abhishek Kumar</au><au>Chaudhari, Anand Kumar</au><au>Deepika</au><au>Dubey, Nawal Kishore</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Eugenol loaded chitosan nanoemulsion for food protection and inhibition of Aflatoxin B 1 synthesizing genes based on molecular docking</atitle><jtitle>Carbohydrate polymers</jtitle><addtitle>Carbohydr Polym</addtitle><date>2021-03-01</date><risdate>2021</risdate><volume>255</volume><spage>117339</spage><pages>117339-</pages><eissn>1879-1344</eissn><abstract>The present investigation entails the fabrication and characterization of nanometric emulsion of eugenol (Nm-eugenol) encompassed into chitosan for assessing bio-efficacy in terms of in vitro antifungal actions, antiaflatoxigenic potential, and in situ preservative efficacy against Aspergillus flavus infestation and aflatoxin B
(AFB
) mediated loss of dietary minerals, lipid triglycerides and alterations in composition of important macronutrients in stored rice. Nm-eugenol characterized by SEM, XRD, and FTIR exhibited biphasic burst release of eugenol. Reduction in ergosterol and methylglyoxal (AFB
-inducer) content after Nm-eugenol fumigation depicted biochemical mechanism of antifungal and antiaflatoxigenic activities. In silico 3D homology docking of eugenol with Ver-1 gene validated molecular mechanism of AFB
inhibition. Further, significant protection of rice seeds from fungi, AFB
contamination and preservation against loss of rice minerals, macronutrients and lipids during storage suggested deployment of chitosan as a biocompatible wall material for eugenol encapsulation and application as novel green preservative for food protection.</abstract><cop>England</cop><pmid>33436182</pmid><doi>10.1016/j.carbpol.2020.117339</doi></addata></record> |
| fulltext | fulltext |
| identifier | EISSN: 1879-1344 |
| ispartof | Carbohydrate polymers, 2021-03, Vol.255, p.117339 |
| issn | 1879-1344 |
| language | eng |
| recordid | cdi_pubmed_primary_33436182 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Aflatoxin B1 - antagonists & inhibitors Aflatoxin B1 - chemistry Aflatoxin B1 - metabolism Aflatoxin B1 - toxicity Anti-Infective Agents - metabolism Anti-Infective Agents - pharmacology Aspergillus flavus - drug effects Aspergillus flavus - growth & development Aspergillus flavus - metabolism Binding Sites Chitosan - chemistry Drug Compounding - methods Drug Liberation Edible Grain Emulsions Ergosterol - antagonists & inhibitors Ergosterol - metabolism Eugenol - metabolism Eugenol - pharmacology Food Preservation - methods Fungal Proteins - antagonists & inhibitors Fungal Proteins - chemistry Fungal Proteins - metabolism Humans Kinetics Molecular Docking Simulation Nanostructures - chemistry Nanostructures - ultrastructure Nutrients - analysis Oryza - drug effects Oryza - microbiology Protein Binding Protein Conformation, alpha-Helical Protein Conformation, beta-Strand Pyruvaldehyde - antagonists & inhibitors Pyruvaldehyde - metabolism Triglycerides - analysis |
| title | Eugenol loaded chitosan nanoemulsion for food protection and inhibition of Aflatoxin B 1 synthesizing genes based on molecular docking |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-23T13%3A45%3A49IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-pubmed&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Eugenol%20loaded%20chitosan%20nanoemulsion%20for%20food%20protection%20and%20inhibition%20of%20Aflatoxin%20B%201%20synthesizing%20genes%20based%20on%20molecular%20docking&rft.jtitle=Carbohydrate%20polymers&rft.au=Das,%20Somenath&rft.date=2021-03-01&rft.volume=255&rft.spage=117339&rft.pages=117339-&rft.eissn=1879-1344&rft_id=info:doi/10.1016/j.carbpol.2020.117339&rft_dat=%3Cpubmed%3E33436182%3C/pubmed%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/33436182&rfr_iscdi=true |