Construction of an Enzyme-Free Initiator-Replicated Hybridization Chain Reaction Circuit for Amplified Methyltransferase Evaluation and Inhibitor Assay

The enzyme-free nucleic acid amplification circuit, for example, hybridization chain reaction (HCR), has paved a broad avenue for evaluating various enzyme-involved biotransformations, including DNA methyltransferases (MTases). The nonenzymatic MTase-sensing platform has supplemented a versatile too...

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Veröffentlicht in:	Analytical chemistry (Washington) 2021-02, Vol.93 (4), p.2403-2410
Hauptverfasser:	Shang, Jinhua, Li, Chunxiao, Li, Fengzhe, Wang, Qing, Yuan, Bifeng, Wang, Fuan
Format:	Artikel
Sprache:	eng
Schlagworte:	Amplification Analytical chemistry Antimetabolites - pharmacology Azacitidine - pharmacology Biological properties Biological samples Biotransformation Chemistry Chemistry, Analytical Circuits Deoxyribonucleic acid Depletion DNA Endonuclease Energy transfer Enzymes Escherichia coli - metabolism Fluorescence resonance energy transfer Fluorouracil - pharmacology Hybridization Initiators Methylation Methyltransferase Methyltransferases - antagonists & inhibitors Methyltransferases - chemistry Methyltransferases - metabolism Nucleic Acid Amplification Techniques - methods Nucleic acids Physical Sciences Replication Science & Technology Substrates
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