Exosomes derived from tendon stem cells promote cell proliferation and migration through the TGF β signal pathway
Tendon stem cells (TSCs) are a kind of progenitor cells found in tendon niches, which play a key role in the repair of tendon injuries. Exosomes that mediate cell communication are involved in physiological processes and various diseases, while the effect of exosomes derived from TSCs (TSC-exo) on T...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2021-01, Vol.536, p.88-94 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Li, Mingda Jia, Jie Li, Shanshan Cui, Baocheng Huang, Jiao Guo, Zhaoming Ma, Kun Wang, Li Cui, Changhao |
| description | Tendon stem cells (TSCs) are a kind of progenitor cells found in tendon niches, which play a key role in the repair of tendon injuries. Exosomes that mediate cell communication are involved in physiological processes and various diseases, while the effect of exosomes derived from TSCs (TSC-exo) on TSCs is still unclear. The purpose of this study is to explore the effect of TSC-exo on TSCs. Analyzing the characteristics of TSC-exo, we found that the TSC-exo were enriched in a large amount of transforming growth factor β (TGF β) by western blotting. We also found that the TGF β carried by TSC-exo can effectively accelerate the proliferation and migration of TSCs. We further found that TGF β carried by TSC-exo can activate the TGF β-Smad2/3 and the ERK1/2 signaling pathway in TSCs. Furthermore, matrix metalloenzyme 2 (MMP2), a downstream molecule of Smad2, is regulated by TGF β carried by TSC-exo. Collectively, our findings provide molecular insights into TSC-exo and indicate that TSC-exo are a potential strategy for treating tendon injuries.
•Tendon stem cells derived exosomes promote proliferation and migration of tendon stem cells.•Tendon stem cells derived exosomes are enriched for TGF β.•TGF β carried by exosomes supports proliferation and migration through the TGF β signaling pathway. |
| doi_str_mv | 10.1016/j.bbrc.2020.12.057 |
| format | Article |
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•Tendon stem cells derived exosomes promote proliferation and migration of tendon stem cells.•Tendon stem cells derived exosomes are enriched for TGF β.•TGF β carried by exosomes supports proliferation and migration through the TGF β signaling pathway.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2020.12.057</identifier><identifier>PMID: 33370718</identifier><language>eng</language><publisher>SAN DIEGO: Elsevier Inc</publisher><subject>Animals ; Biochemistry & Molecular Biology ; Biophysics ; Cell Movement ; Cell Proliferation ; Exosomes ; Exosomes - metabolism ; Exosomes - ultrastructure ; Extracellular Signal-Regulated MAP Kinases - metabolism ; Life Sciences & Biomedicine ; Migration ; Proliferation ; Rats ; Rats, Sprague-Dawley ; Science & Technology ; Signal Transduction ; Smad Proteins - metabolism ; Stem Cells - cytology ; Tendon stem cells ; Tendons - cytology ; TGF β ; Transforming Growth Factor beta - metabolism</subject><ispartof>Biochemical and biophysical research communications, 2021-01, Vol.536, p.88-94</ispartof><rights>2020 Elsevier Inc.</rights><rights>Copyright © 2020 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>true</woscitedreferencessubscribed><woscitedreferencescount>20</woscitedreferencescount><woscitedreferencesoriginalsourcerecordid>wos000609033200014</woscitedreferencesoriginalsourcerecordid><citedby>FETCH-LOGICAL-c356t-f4404270d32659368729f46099129ffe437d0085c9f233073bf5729a098bdc4a3</citedby><cites>FETCH-LOGICAL-c356t-f4404270d32659368729f46099129ffe437d0085c9f233073bf5729a098bdc4a3</cites><orcidid>0000-0001-5800-1303</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bbrc.2020.12.057$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,782,786,3554,27933,27934,39267,46004</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33370718$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Mingda</creatorcontrib><creatorcontrib>Jia, Jie</creatorcontrib><creatorcontrib>Li, Shanshan</creatorcontrib><creatorcontrib>Cui, Baocheng</creatorcontrib><creatorcontrib>Huang, Jiao</creatorcontrib><creatorcontrib>Guo, Zhaoming</creatorcontrib><creatorcontrib>Ma, Kun</creatorcontrib><creatorcontrib>Wang, Li</creatorcontrib><creatorcontrib>Cui, Changhao</creatorcontrib><title>Exosomes derived from tendon stem cells promote cell proliferation and migration through the TGF β signal pathway</title><title>Biochemical and biophysical research communications</title><addtitle>BIOCHEM BIOPH RES CO</addtitle><addtitle>Biochem Biophys Res Commun</addtitle><description>Tendon stem cells (TSCs) are a kind of progenitor cells found in tendon niches, which play a key role in the repair of tendon injuries. Exosomes that mediate cell communication are involved in physiological processes and various diseases, while the effect of exosomes derived from TSCs (TSC-exo) on TSCs is still unclear. The purpose of this study is to explore the effect of TSC-exo on TSCs. Analyzing the characteristics of TSC-exo, we found that the TSC-exo were enriched in a large amount of transforming growth factor β (TGF β) by western blotting. We also found that the TGF β carried by TSC-exo can effectively accelerate the proliferation and migration of TSCs. We further found that TGF β carried by TSC-exo can activate the TGF β-Smad2/3 and the ERK1/2 signaling pathway in TSCs. Furthermore, matrix metalloenzyme 2 (MMP2), a downstream molecule of Smad2, is regulated by TGF β carried by TSC-exo. Collectively, our findings provide molecular insights into TSC-exo and indicate that TSC-exo are a potential strategy for treating tendon injuries.
•Tendon stem cells derived exosomes promote proliferation and migration of tendon stem cells.•Tendon stem cells derived exosomes are enriched for TGF β.•TGF β carried by exosomes supports proliferation and migration through the TGF β signaling pathway.</description><subject>Animals</subject><subject>Biochemistry & Molecular Biology</subject><subject>Biophysics</subject><subject>Cell Movement</subject><subject>Cell Proliferation</subject><subject>Exosomes</subject><subject>Exosomes - metabolism</subject><subject>Exosomes - ultrastructure</subject><subject>Extracellular Signal-Regulated MAP Kinases - metabolism</subject><subject>Life Sciences & Biomedicine</subject><subject>Migration</subject><subject>Proliferation</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Science & Technology</subject><subject>Signal Transduction</subject><subject>Smad Proteins - metabolism</subject><subject>Stem Cells - cytology</subject><subject>Tendon stem cells</subject><subject>Tendons - cytology</subject><subject>TGF β</subject><subject>Transforming Growth Factor beta - metabolism</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>HGBXW</sourceid><sourceid>EIF</sourceid><recordid>eNqNkclqHDEQhkVIiCdOXiCHoGMg9KS09Aa5hMF2AgZfbPBNqFulGQ3TLUdSe3mtPIifyWrPxMfgUy18f1H1FyGfGSwZsOr7dtl1oV9y4LnBl1DWb8iCQQsFZyDfkgUAVAVv2fUR-RDjFoAxWbXvyZEQooaaNQsSTu599ANGajC4WzTUBj_QhKPxI40JB9rjbhfpTW77hM_VXOycxaCTy5QeDR3c-lClTfDTepMj0suzU_r4l0a3HnVW6bS50w8fyTurdxE_HeIxuTo9uVz9Ks4vzn6vfp4XvSirVFgpQfIajOBV2YqqqXlrZQVty3JiUYraADRl31ouBNSis2VGNLRNZ3qpxTH5up-bt_0zYUxqcHFeX4_op6i4rEUtoZEso3yP9sHHGNCqm-AGHR4UAzV7rbZq9lrNXivGVfY6i74c5k_dgOZF8s_cDDR74A47b2PvcOzxBZufk38lBM8ZkyuXnv1b-WlMWfrt9dJM_9jTmO28dRjUQWFcwD4p493_DnkC2oyxRw</recordid><startdate>20210115</startdate><enddate>20210115</enddate><creator>Li, Mingda</creator><creator>Jia, Jie</creator><creator>Li, Shanshan</creator><creator>Cui, Baocheng</creator><creator>Huang, Jiao</creator><creator>Guo, Zhaoming</creator><creator>Ma, Kun</creator><creator>Wang, Li</creator><creator>Cui, Changhao</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>BLEPL</scope><scope>DTL</scope><scope>HGBXW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-5800-1303</orcidid></search><sort><creationdate>20210115</creationdate><title>Exosomes derived from tendon stem cells promote cell proliferation and migration through the TGF β signal pathway</title><author>Li, Mingda ; Jia, Jie ; Li, Shanshan ; Cui, Baocheng ; Huang, Jiao ; Guo, Zhaoming ; Ma, Kun ; Wang, Li ; Cui, Changhao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-f4404270d32659368729f46099129ffe437d0085c9f233073bf5729a098bdc4a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Animals</topic><topic>Biochemistry & Molecular Biology</topic><topic>Biophysics</topic><topic>Cell Movement</topic><topic>Cell Proliferation</topic><topic>Exosomes</topic><topic>Exosomes - metabolism</topic><topic>Exosomes - ultrastructure</topic><topic>Extracellular Signal-Regulated MAP Kinases - metabolism</topic><topic>Life Sciences & Biomedicine</topic><topic>Migration</topic><topic>Proliferation</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Science & Technology</topic><topic>Signal Transduction</topic><topic>Smad Proteins - metabolism</topic><topic>Stem Cells - cytology</topic><topic>Tendon stem cells</topic><topic>Tendons - cytology</topic><topic>TGF β</topic><topic>Transforming Growth Factor beta - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Mingda</creatorcontrib><creatorcontrib>Jia, Jie</creatorcontrib><creatorcontrib>Li, Shanshan</creatorcontrib><creatorcontrib>Cui, Baocheng</creatorcontrib><creatorcontrib>Huang, Jiao</creatorcontrib><creatorcontrib>Guo, Zhaoming</creatorcontrib><creatorcontrib>Ma, Kun</creatorcontrib><creatorcontrib>Wang, Li</creatorcontrib><creatorcontrib>Cui, Changhao</creatorcontrib><collection>Web of Science Core Collection</collection><collection>Science Citation Index Expanded</collection><collection>Web of Science - Science Citation Index Expanded - 2021</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Mingda</au><au>Jia, Jie</au><au>Li, Shanshan</au><au>Cui, Baocheng</au><au>Huang, Jiao</au><au>Guo, Zhaoming</au><au>Ma, Kun</au><au>Wang, Li</au><au>Cui, Changhao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Exosomes derived from tendon stem cells promote cell proliferation and migration through the TGF β signal pathway</atitle><jtitle>Biochemical and biophysical research communications</jtitle><stitle>BIOCHEM BIOPH RES CO</stitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2021-01-15</date><risdate>2021</risdate><volume>536</volume><spage>88</spage><epage>94</epage><pages>88-94</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Tendon stem cells (TSCs) are a kind of progenitor cells found in tendon niches, which play a key role in the repair of tendon injuries. Exosomes that mediate cell communication are involved in physiological processes and various diseases, while the effect of exosomes derived from TSCs (TSC-exo) on TSCs is still unclear. The purpose of this study is to explore the effect of TSC-exo on TSCs. Analyzing the characteristics of TSC-exo, we found that the TSC-exo were enriched in a large amount of transforming growth factor β (TGF β) by western blotting. We also found that the TGF β carried by TSC-exo can effectively accelerate the proliferation and migration of TSCs. We further found that TGF β carried by TSC-exo can activate the TGF β-Smad2/3 and the ERK1/2 signaling pathway in TSCs. Furthermore, matrix metalloenzyme 2 (MMP2), a downstream molecule of Smad2, is regulated by TGF β carried by TSC-exo. Collectively, our findings provide molecular insights into TSC-exo and indicate that TSC-exo are a potential strategy for treating tendon injuries.
•Tendon stem cells derived exosomes promote proliferation and migration of tendon stem cells.•Tendon stem cells derived exosomes are enriched for TGF β.•TGF β carried by exosomes supports proliferation and migration through the TGF β signaling pathway.</abstract><cop>SAN DIEGO</cop><pub>Elsevier Inc</pub><pmid>33370718</pmid><doi>10.1016/j.bbrc.2020.12.057</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0001-5800-1303</orcidid></addata></record> |
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| source | MEDLINE; Web of Science - Science Citation Index Expanded - 2021<img src="https://exlibris-pub.s3.amazonaws.com/fromwos-v2.jpg" />; Access via ScienceDirect (Elsevier) |
| subjects | Animals Biochemistry & Molecular Biology Biophysics Cell Movement Cell Proliferation Exosomes Exosomes - metabolism Exosomes - ultrastructure Extracellular Signal-Regulated MAP Kinases - metabolism Life Sciences & Biomedicine Migration Proliferation Rats Rats, Sprague-Dawley Science & Technology Signal Transduction Smad Proteins - metabolism Stem Cells - cytology Tendon stem cells Tendons - cytology TGF β Transforming Growth Factor beta - metabolism |
| title | Exosomes derived from tendon stem cells promote cell proliferation and migration through the TGF β signal pathway |
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