A novel NIR fluorescence probe with cysteine-activated structure for specific detection of cysteine and its application in vitro and in vivo
Cysteine (Cys) as a vital antioxidant molecule and an effective biomarker for illness, plays an essential role in physiological functions and pathological processes. Extensive work has been done to explore the physiological functions of Cys and develop probes for detection of biothiols. However, the...
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| Veröffentlicht in: | Talanta (Oxford) 2021-02, Vol.223 (Pt 2), p.121758-121758, Article 121758 |
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| creator | Ge, Chunpo Shen, Fengge Yin, Yanyan Chang, Kaiwen Zhang, Xuan Zhou, Pingxin Li, Junmei Liu, Youxun Lu, Chengbiao |
| description | Cysteine (Cys) as a vital antioxidant molecule and an effective biomarker for illness, plays an essential role in physiological functions and pathological processes. Extensive work has been done to explore the physiological functions of Cys and develop probes for detection of biothiols. However, the challenge to differentiate Cys from glutathione and homocystine remains. In this work, we constructed a novel near-infrared (NIR) probe, termed TMN-Cys, using TMN-NH2 and thionoesters. The probe could selectively detect Cys over homocysteine and glutathione in solution. It displayed a large Stokes shift (210 nm) upon treatment with Cys, and its detection limit was as low as 79 nM. Moreover, this probe showed low toxicity and was successfully employed in monitoring endogenous Cys in living cells and mice.
[Display omitted]
•TMN-Cys, a novel red-emitting fluorescent probe based onthionoesters, was designed.•TMN-Cys selectively monitored Cys both in vitro and in vivo.•TMN-Cys exhibited high sensitivity and a large Strokes shift after reacting with Cys.•The detection limit of TMN-Cys was as low as 79 nM. |
| doi_str_mv | 10.1016/j.talanta.2020.121758 |
| format | Article |
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[Display omitted]
•TMN-Cys, a novel red-emitting fluorescent probe based onthionoesters, was designed.•TMN-Cys selectively monitored Cys both in vitro and in vivo.•TMN-Cys exhibited high sensitivity and a large Strokes shift after reacting with Cys.•The detection limit of TMN-Cys was as low as 79 nM.</description><identifier>ISSN: 0039-9140</identifier><identifier>EISSN: 1873-3573</identifier><identifier>DOI: 10.1016/j.talanta.2020.121758</identifier><identifier>PMID: 33298274</identifier><language>eng</language><publisher>AMSTERDAM: Elsevier B.V</publisher><subject>Animals ; Chemistry ; Chemistry, Analytical ; Cysteine ; Cysteine detection ; Fluorescent Dyes ; Glutathione ; H2S donors ; Homocysteine ; Large Stokes shift ; Limit of Detection ; Living cells imaging ; Mice ; NIR fluroescence Probe ; Physical Sciences ; Science & Technology ; Thionoesters</subject><ispartof>Talanta (Oxford), 2021-02, Vol.223 (Pt 2), p.121758-121758, Article 121758</ispartof><rights>2020 Elsevier B.V.</rights><rights>Copyright © 2020 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>true</woscitedreferencessubscribed><woscitedreferencescount>18</woscitedreferencescount><woscitedreferencesoriginalsourcerecordid>wos000599844700011</woscitedreferencesoriginalsourcerecordid><citedby>FETCH-LOGICAL-c365t-e4805d8c4c1e15c641a880fdb70b58490ac19da421754464c31d77b0164151f03</citedby><cites>FETCH-LOGICAL-c365t-e4805d8c4c1e15c641a880fdb70b58490ac19da421754464c31d77b0164151f03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.talanta.2020.121758$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,39263,46000</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33298274$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ge, Chunpo</creatorcontrib><creatorcontrib>Shen, Fengge</creatorcontrib><creatorcontrib>Yin, Yanyan</creatorcontrib><creatorcontrib>Chang, Kaiwen</creatorcontrib><creatorcontrib>Zhang, Xuan</creatorcontrib><creatorcontrib>Zhou, Pingxin</creatorcontrib><creatorcontrib>Li, Junmei</creatorcontrib><creatorcontrib>Liu, Youxun</creatorcontrib><creatorcontrib>Lu, Chengbiao</creatorcontrib><title>A novel NIR fluorescence probe with cysteine-activated structure for specific detection of cysteine and its application in vitro and in vivo</title><title>Talanta (Oxford)</title><addtitle>TALANTA</addtitle><addtitle>Talanta</addtitle><description>Cysteine (Cys) as a vital antioxidant molecule and an effective biomarker for illness, plays an essential role in physiological functions and pathological processes. Extensive work has been done to explore the physiological functions of Cys and develop probes for detection of biothiols. However, the challenge to differentiate Cys from glutathione and homocystine remains. In this work, we constructed a novel near-infrared (NIR) probe, termed TMN-Cys, using TMN-NH2 and thionoesters. The probe could selectively detect Cys over homocysteine and glutathione in solution. It displayed a large Stokes shift (210 nm) upon treatment with Cys, and its detection limit was as low as 79 nM. Moreover, this probe showed low toxicity and was successfully employed in monitoring endogenous Cys in living cells and mice.
[Display omitted]
•TMN-Cys, a novel red-emitting fluorescent probe based onthionoesters, was designed.•TMN-Cys selectively monitored Cys both in vitro and in vivo.•TMN-Cys exhibited high sensitivity and a large Strokes shift after reacting with Cys.•The detection limit of TMN-Cys was as low as 79 nM.</description><subject>Animals</subject><subject>Chemistry</subject><subject>Chemistry, Analytical</subject><subject>Cysteine</subject><subject>Cysteine detection</subject><subject>Fluorescent Dyes</subject><subject>Glutathione</subject><subject>H2S donors</subject><subject>Homocysteine</subject><subject>Large Stokes shift</subject><subject>Limit of Detection</subject><subject>Living cells imaging</subject><subject>Mice</subject><subject>NIR fluroescence Probe</subject><subject>Physical Sciences</subject><subject>Science & Technology</subject><subject>Thionoesters</subject><issn>0039-9140</issn><issn>1873-3573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>HGBXW</sourceid><sourceid>EIF</sourceid><recordid>eNqNkc1u1DAUhS1ERYfCI4C8REKZ2rGT2CtUjQqtVFGpgrXl2DfCo0wcbCdV34GHxmmms4WVf-53bJ1zEPpAyZYSWl_ut0n3ekh6W5Iy35W0qcQrtKGiYQWrGvYabQhhspCUk3P0NsY9IaRkhL1B54yVUpQN36A_V3jwM_T4--0D7vrJB4gGBgN4DL4F_OjSL2yeYgI3QKFNcrNOYHFMYTJpCoA7H3AcwbjOGWwhQWb8gH13kmE9WOxSxHoce2f089wNeHYp-HW4HGb_Dp11uo_w_rheoJ9fr3_sboq7-2-3u6u7wrC6SgVwQSorDDcUaGVqTrUQpLNtQ9pKcEm0odJqvkTCec0No7Zp2pwapxXtCLtAn9Z3s8ffE8SkDi677nOe4KeoSl5LIpmgMqPViprgYwzQqTG4gw5PihK1FKH26liEWopQaxFZ9_H4xdQewJ5UL8ln4PMKPELru2jcEvoJy1VVUgrOm7yjNNPi_-mdS88Z7_w0pCz9skohJzo7COooty7krpT17h9e_gJHZL5m</recordid><startdate>20210201</startdate><enddate>20210201</enddate><creator>Ge, Chunpo</creator><creator>Shen, Fengge</creator><creator>Yin, Yanyan</creator><creator>Chang, Kaiwen</creator><creator>Zhang, Xuan</creator><creator>Zhou, Pingxin</creator><creator>Li, Junmei</creator><creator>Liu, Youxun</creator><creator>Lu, Chengbiao</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>BLEPL</scope><scope>DTL</scope><scope>HGBXW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20210201</creationdate><title>A novel NIR fluorescence probe with cysteine-activated structure for specific detection of cysteine and its application in vitro and in vivo</title><author>Ge, Chunpo ; Shen, Fengge ; Yin, Yanyan ; Chang, Kaiwen ; Zhang, Xuan ; Zhou, Pingxin ; Li, Junmei ; Liu, Youxun ; Lu, Chengbiao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c365t-e4805d8c4c1e15c641a880fdb70b58490ac19da421754464c31d77b0164151f03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Animals</topic><topic>Chemistry</topic><topic>Chemistry, Analytical</topic><topic>Cysteine</topic><topic>Cysteine detection</topic><topic>Fluorescent Dyes</topic><topic>Glutathione</topic><topic>H2S donors</topic><topic>Homocysteine</topic><topic>Large Stokes shift</topic><topic>Limit of Detection</topic><topic>Living cells imaging</topic><topic>Mice</topic><topic>NIR fluroescence Probe</topic><topic>Physical Sciences</topic><topic>Science & Technology</topic><topic>Thionoesters</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ge, Chunpo</creatorcontrib><creatorcontrib>Shen, Fengge</creatorcontrib><creatorcontrib>Yin, Yanyan</creatorcontrib><creatorcontrib>Chang, Kaiwen</creatorcontrib><creatorcontrib>Zhang, Xuan</creatorcontrib><creatorcontrib>Zhou, Pingxin</creatorcontrib><creatorcontrib>Li, Junmei</creatorcontrib><creatorcontrib>Liu, Youxun</creatorcontrib><creatorcontrib>Lu, Chengbiao</creatorcontrib><collection>Web of Science Core Collection</collection><collection>Science Citation Index Expanded</collection><collection>Web of Science - Science Citation Index Expanded - 2021</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Talanta (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ge, Chunpo</au><au>Shen, Fengge</au><au>Yin, Yanyan</au><au>Chang, Kaiwen</au><au>Zhang, Xuan</au><au>Zhou, Pingxin</au><au>Li, Junmei</au><au>Liu, Youxun</au><au>Lu, Chengbiao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A novel NIR fluorescence probe with cysteine-activated structure for specific detection of cysteine and its application in vitro and in vivo</atitle><jtitle>Talanta (Oxford)</jtitle><stitle>TALANTA</stitle><addtitle>Talanta</addtitle><date>2021-02-01</date><risdate>2021</risdate><volume>223</volume><issue>Pt 2</issue><spage>121758</spage><epage>121758</epage><pages>121758-121758</pages><artnum>121758</artnum><issn>0039-9140</issn><eissn>1873-3573</eissn><abstract>Cysteine (Cys) as a vital antioxidant molecule and an effective biomarker for illness, plays an essential role in physiological functions and pathological processes. Extensive work has been done to explore the physiological functions of Cys and develop probes for detection of biothiols. However, the challenge to differentiate Cys from glutathione and homocystine remains. In this work, we constructed a novel near-infrared (NIR) probe, termed TMN-Cys, using TMN-NH2 and thionoesters. The probe could selectively detect Cys over homocysteine and glutathione in solution. It displayed a large Stokes shift (210 nm) upon treatment with Cys, and its detection limit was as low as 79 nM. Moreover, this probe showed low toxicity and was successfully employed in monitoring endogenous Cys in living cells and mice.
[Display omitted]
•TMN-Cys, a novel red-emitting fluorescent probe based onthionoesters, was designed.•TMN-Cys selectively monitored Cys both in vitro and in vivo.•TMN-Cys exhibited high sensitivity and a large Strokes shift after reacting with Cys.•The detection limit of TMN-Cys was as low as 79 nM.</abstract><cop>AMSTERDAM</cop><pub>Elsevier B.V</pub><pmid>33298274</pmid><doi>10.1016/j.talanta.2020.121758</doi><tpages>7</tpages></addata></record> |
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| subjects | Animals Chemistry Chemistry, Analytical Cysteine Cysteine detection Fluorescent Dyes Glutathione H2S donors Homocysteine Large Stokes shift Limit of Detection Living cells imaging Mice NIR fluroescence Probe Physical Sciences Science & Technology Thionoesters |
| title | A novel NIR fluorescence probe with cysteine-activated structure for specific detection of cysteine and its application in vitro and in vivo |
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