Immuno-modulatory effects of methanolic extract of Ferula szowitsiana on isolated human Th 1 /Th 2 /T reg cytokines levels, and their genes expression and nitric oxide production
Anti-inflammatory and anti-oxidants activities of Ferula szowitsiana L. (F. szowitsiana) were shown in ancient texts and assayed by modern studies. However, immunomodulatory properties of the plant are poorly understood. The effects of F. szowitsiana extract (10, 40 and 160 µg/ml), dexamethasone and...
Gespeichert in:
| Veröffentlicht in: | Cytokine (Philadelphia, Pa.) Pa.), 2021-02, Vol.138, p.155387 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | 155387 |
| container_title | Cytokine (Philadelphia, Pa.) |
| container_volume | 138 |
| creator | Askari, Vahid Reza Alavinezhad, Azam Rahimi, Vafa Baradaran Rezaee, Seyyed Abdorrahim Boskabady, Mohammad Hossein |
| description | Anti-inflammatory and anti-oxidants activities of Ferula szowitsiana L. (F. szowitsiana) were shown in ancient texts and assayed by modern studies. However, immunomodulatory properties of the plant are poorly understood.
The effects of F. szowitsiana extract (10, 40 and 160 µg/ml), dexamethasone and vehicle were investigated on nitric oxide (NO) level, cell proliferation, and cytokines (IL-4, IL10 and IFN-γ) expression at gene and protein levels in non-stimulated and phytohaemagglutinin-stimulated human lymphocytes (n = 15 in each group).
Cell proliferation, cytokines secretion, NO production and levels of genes expression were significantly inhibited but IFN-γ/IL-4 and IL-10/IL-4 ratios (T helper 1/Th2 and Treg/Th2 balances respectively) were increased by dexamethasone and all three concentrations of the extract compared to control group in stimulated lymphocytes (P |
| doi_str_mv | 10.1016/j.cyto.2020.155387 |
| format | Article |
| fullrecord | <record><control><sourceid>pubmed</sourceid><recordid>TN_cdi_pubmed_primary_33278664</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>33278664</sourcerecordid><originalsourceid>FETCH-pubmed_primary_332786643</originalsourceid><addsrcrecordid>eNqFT8tOwzAQtJAQLY8f4ID2A0jqB03aM6Ki994rk2wal9iOvA4kfBZfiCPBmcusNDOanWHsXvBccFGsznk1RZ9LLhOxXqtNecGWgm-LjHOpFuya6Mw536qyvGILpWS5KYqnJfveWzs4n1lfD52OPkyATYNVJPANWIytdr4zFeAYg67izO4wJC_Ql_80kYx2GrwDQz4FYA3tYLWDQwsCVgllQgh4grngu3FI0OEHdvQI2tUQWzQBTjjzOPYBiUxKmyVnYkif_WhqhD6khlVM2i27bHRHePd7b9jD7uXw_Jr1w5vF-tgHY3WYjn8j1b-GHw56ZrM</addsrcrecordid><sourcetype>Index Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Immuno-modulatory effects of methanolic extract of Ferula szowitsiana on isolated human Th 1 /Th 2 /T reg cytokines levels, and their genes expression and nitric oxide production</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Askari, Vahid Reza ; Alavinezhad, Azam ; Rahimi, Vafa Baradaran ; Rezaee, Seyyed Abdorrahim ; Boskabady, Mohammad Hossein</creator><creatorcontrib>Askari, Vahid Reza ; Alavinezhad, Azam ; Rahimi, Vafa Baradaran ; Rezaee, Seyyed Abdorrahim ; Boskabady, Mohammad Hossein</creatorcontrib><description>Anti-inflammatory and anti-oxidants activities of Ferula szowitsiana L. (F. szowitsiana) were shown in ancient texts and assayed by modern studies. However, immunomodulatory properties of the plant are poorly understood.
The effects of F. szowitsiana extract (10, 40 and 160 µg/ml), dexamethasone and vehicle were investigated on nitric oxide (NO) level, cell proliferation, and cytokines (IL-4, IL10 and IFN-γ) expression at gene and protein levels in non-stimulated and phytohaemagglutinin-stimulated human lymphocytes (n = 15 in each group).
Cell proliferation, cytokines secretion, NO production and levels of genes expression were significantly inhibited but IFN-γ/IL-4 and IL-10/IL-4 ratios (T helper 1/Th2 and Treg/Th2 balances respectively) were increased by dexamethasone and all three concentrations of the extract compared to control group in stimulated lymphocytes (P < 0.001 for all cases). The effect of three concentrations of the extract in all experiments was significantly lower than dexamethasone (P < 0.001 for all cases).
The extract of F. szowitsiana concentration-dependently decreased NO level but increased Th
/Th
and T
/Th
ratios toward Th
and T
. These results suggest the therapeutic potential of the plant's extract in inflammatory diseases with dominant Th2 polarization such as asthma or cancers.</description><identifier>EISSN: 1096-0023</identifier><identifier>DOI: 10.1016/j.cyto.2020.155387</identifier><identifier>PMID: 33278664</identifier><language>eng</language><publisher>England</publisher><subject>Antioxidants - metabolism ; Cell Proliferation ; Cytokines - metabolism ; Dexamethasone - pharmacology ; Ferula - metabolism ; Gene Expression - drug effects ; Gene Expression Profiling ; Gene Expression Regulation - drug effects ; Humans ; Immunomodulation ; Interferon-gamma - biosynthesis ; Interleukin-10 - biosynthesis ; Interleukin-4 - biosynthesis ; Lymphocyte Activation - drug effects ; Methanol - chemistry ; Nitric Oxide - metabolism ; Plant Extracts - pharmacology ; T-Lymphocytes, Regulatory - cytology ; Th1 Cells - metabolism ; Th2 Cells - metabolism</subject><ispartof>Cytokine (Philadelphia, Pa.), 2021-02, Vol.138, p.155387</ispartof><rights>Copyright © 2020 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33278664$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Askari, Vahid Reza</creatorcontrib><creatorcontrib>Alavinezhad, Azam</creatorcontrib><creatorcontrib>Rahimi, Vafa Baradaran</creatorcontrib><creatorcontrib>Rezaee, Seyyed Abdorrahim</creatorcontrib><creatorcontrib>Boskabady, Mohammad Hossein</creatorcontrib><title>Immuno-modulatory effects of methanolic extract of Ferula szowitsiana on isolated human Th 1 /Th 2 /T reg cytokines levels, and their genes expression and nitric oxide production</title><title>Cytokine (Philadelphia, Pa.)</title><addtitle>Cytokine</addtitle><description>Anti-inflammatory and anti-oxidants activities of Ferula szowitsiana L. (F. szowitsiana) were shown in ancient texts and assayed by modern studies. However, immunomodulatory properties of the plant are poorly understood.
The effects of F. szowitsiana extract (10, 40 and 160 µg/ml), dexamethasone and vehicle were investigated on nitric oxide (NO) level, cell proliferation, and cytokines (IL-4, IL10 and IFN-γ) expression at gene and protein levels in non-stimulated and phytohaemagglutinin-stimulated human lymphocytes (n = 15 in each group).
Cell proliferation, cytokines secretion, NO production and levels of genes expression were significantly inhibited but IFN-γ/IL-4 and IL-10/IL-4 ratios (T helper 1/Th2 and Treg/Th2 balances respectively) were increased by dexamethasone and all three concentrations of the extract compared to control group in stimulated lymphocytes (P < 0.001 for all cases). The effect of three concentrations of the extract in all experiments was significantly lower than dexamethasone (P < 0.001 for all cases).
The extract of F. szowitsiana concentration-dependently decreased NO level but increased Th
/Th
and T
/Th
ratios toward Th
and T
. These results suggest the therapeutic potential of the plant's extract in inflammatory diseases with dominant Th2 polarization such as asthma or cancers.</description><subject>Antioxidants - metabolism</subject><subject>Cell Proliferation</subject><subject>Cytokines - metabolism</subject><subject>Dexamethasone - pharmacology</subject><subject>Ferula - metabolism</subject><subject>Gene Expression - drug effects</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Humans</subject><subject>Immunomodulation</subject><subject>Interferon-gamma - biosynthesis</subject><subject>Interleukin-10 - biosynthesis</subject><subject>Interleukin-4 - biosynthesis</subject><subject>Lymphocyte Activation - drug effects</subject><subject>Methanol - chemistry</subject><subject>Nitric Oxide - metabolism</subject><subject>Plant Extracts - pharmacology</subject><subject>T-Lymphocytes, Regulatory - cytology</subject><subject>Th1 Cells - metabolism</subject><subject>Th2 Cells - metabolism</subject><issn>1096-0023</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFT8tOwzAQtJAQLY8f4ID2A0jqB03aM6Ki994rk2wal9iOvA4kfBZfiCPBmcusNDOanWHsXvBccFGsznk1RZ9LLhOxXqtNecGWgm-LjHOpFuya6Mw536qyvGILpWS5KYqnJfveWzs4n1lfD52OPkyATYNVJPANWIytdr4zFeAYg67izO4wJC_Ql_80kYx2GrwDQz4FYA3tYLWDQwsCVgllQgh4grngu3FI0OEHdvQI2tUQWzQBTjjzOPYBiUxKmyVnYkif_WhqhD6khlVM2i27bHRHePd7b9jD7uXw_Jr1w5vF-tgHY3WYjn8j1b-GHw56ZrM</recordid><startdate>202102</startdate><enddate>202102</enddate><creator>Askari, Vahid Reza</creator><creator>Alavinezhad, Azam</creator><creator>Rahimi, Vafa Baradaran</creator><creator>Rezaee, Seyyed Abdorrahim</creator><creator>Boskabady, Mohammad Hossein</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>202102</creationdate><title>Immuno-modulatory effects of methanolic extract of Ferula szowitsiana on isolated human Th 1 /Th 2 /T reg cytokines levels, and their genes expression and nitric oxide production</title><author>Askari, Vahid Reza ; Alavinezhad, Azam ; Rahimi, Vafa Baradaran ; Rezaee, Seyyed Abdorrahim ; Boskabady, Mohammad Hossein</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-pubmed_primary_332786643</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Antioxidants - metabolism</topic><topic>Cell Proliferation</topic><topic>Cytokines - metabolism</topic><topic>Dexamethasone - pharmacology</topic><topic>Ferula - metabolism</topic><topic>Gene Expression - drug effects</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Humans</topic><topic>Immunomodulation</topic><topic>Interferon-gamma - biosynthesis</topic><topic>Interleukin-10 - biosynthesis</topic><topic>Interleukin-4 - biosynthesis</topic><topic>Lymphocyte Activation - drug effects</topic><topic>Methanol - chemistry</topic><topic>Nitric Oxide - metabolism</topic><topic>Plant Extracts - pharmacology</topic><topic>T-Lymphocytes, Regulatory - cytology</topic><topic>Th1 Cells - metabolism</topic><topic>Th2 Cells - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Askari, Vahid Reza</creatorcontrib><creatorcontrib>Alavinezhad, Azam</creatorcontrib><creatorcontrib>Rahimi, Vafa Baradaran</creatorcontrib><creatorcontrib>Rezaee, Seyyed Abdorrahim</creatorcontrib><creatorcontrib>Boskabady, Mohammad Hossein</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>Cytokine (Philadelphia, Pa.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Askari, Vahid Reza</au><au>Alavinezhad, Azam</au><au>Rahimi, Vafa Baradaran</au><au>Rezaee, Seyyed Abdorrahim</au><au>Boskabady, Mohammad Hossein</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immuno-modulatory effects of methanolic extract of Ferula szowitsiana on isolated human Th 1 /Th 2 /T reg cytokines levels, and their genes expression and nitric oxide production</atitle><jtitle>Cytokine (Philadelphia, Pa.)</jtitle><addtitle>Cytokine</addtitle><date>2021-02</date><risdate>2021</risdate><volume>138</volume><spage>155387</spage><pages>155387-</pages><eissn>1096-0023</eissn><abstract>Anti-inflammatory and anti-oxidants activities of Ferula szowitsiana L. (F. szowitsiana) were shown in ancient texts and assayed by modern studies. However, immunomodulatory properties of the plant are poorly understood.
The effects of F. szowitsiana extract (10, 40 and 160 µg/ml), dexamethasone and vehicle were investigated on nitric oxide (NO) level, cell proliferation, and cytokines (IL-4, IL10 and IFN-γ) expression at gene and protein levels in non-stimulated and phytohaemagglutinin-stimulated human lymphocytes (n = 15 in each group).
Cell proliferation, cytokines secretion, NO production and levels of genes expression were significantly inhibited but IFN-γ/IL-4 and IL-10/IL-4 ratios (T helper 1/Th2 and Treg/Th2 balances respectively) were increased by dexamethasone and all three concentrations of the extract compared to control group in stimulated lymphocytes (P < 0.001 for all cases). The effect of three concentrations of the extract in all experiments was significantly lower than dexamethasone (P < 0.001 for all cases).
The extract of F. szowitsiana concentration-dependently decreased NO level but increased Th
/Th
and T
/Th
ratios toward Th
and T
. These results suggest the therapeutic potential of the plant's extract in inflammatory diseases with dominant Th2 polarization such as asthma or cancers.</abstract><cop>England</cop><pmid>33278664</pmid><doi>10.1016/j.cyto.2020.155387</doi></addata></record> |
| fulltext | fulltext |
| identifier | EISSN: 1096-0023 |
| ispartof | Cytokine (Philadelphia, Pa.), 2021-02, Vol.138, p.155387 |
| issn | 1096-0023 |
| language | eng |
| recordid | cdi_pubmed_primary_33278664 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Antioxidants - metabolism Cell Proliferation Cytokines - metabolism Dexamethasone - pharmacology Ferula - metabolism Gene Expression - drug effects Gene Expression Profiling Gene Expression Regulation - drug effects Humans Immunomodulation Interferon-gamma - biosynthesis Interleukin-10 - biosynthesis Interleukin-4 - biosynthesis Lymphocyte Activation - drug effects Methanol - chemistry Nitric Oxide - metabolism Plant Extracts - pharmacology T-Lymphocytes, Regulatory - cytology Th1 Cells - metabolism Th2 Cells - metabolism |
| title | Immuno-modulatory effects of methanolic extract of Ferula szowitsiana on isolated human Th 1 /Th 2 /T reg cytokines levels, and their genes expression and nitric oxide production |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-02T08%3A25%3A43IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-pubmed&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Immuno-modulatory%20effects%20of%20methanolic%20extract%20of%20Ferula%20szowitsiana%20on%20isolated%20human%20Th%201%20/Th%202%20/T%20reg%20cytokines%20levels,%20and%20their%20genes%20expression%20and%20nitric%20oxide%20production&rft.jtitle=Cytokine%20(Philadelphia,%20Pa.)&rft.au=Askari,%20Vahid%20Reza&rft.date=2021-02&rft.volume=138&rft.spage=155387&rft.pages=155387-&rft.eissn=1096-0023&rft_id=info:doi/10.1016/j.cyto.2020.155387&rft_dat=%3Cpubmed%3E33278664%3C/pubmed%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/33278664&rfr_iscdi=true |