High Concentrations of Aspartame Induce Pro-Angiogenic Effects in Ovo and Cytotoxic Effects in HT-29 Human Colorectal Carcinoma Cells
Aspartame (ASP), an artificial sweetener abundantly consumed in recent years in an array of dietary products, has raised some concerns in terms of toxicity, and it was even suggested a link with the risk of carcinogenesis (colorectal cancer), though the present scientific data are rather inconclusiv...
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| Veröffentlicht in: | Nutrients 2020-11, Vol.12 (12), p.3600, Article 3600 |
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| creator | Maghiari, Anca Laura Coricovac, Dorina Pinzaru, Iulia Andreea Macasoi, Ioana Gabriela Marcovici, Iasmina Simu, Sebastian Navolan, Dan Dehelean, Cristina |
| description | Aspartame (ASP), an artificial sweetener abundantly consumed in recent years in an array of dietary products, has raised some concerns in terms of toxicity, and it was even suggested a link with the risk of carcinogenesis (colorectal cancer), though the present scientific data are rather inconclusive. This study aims at investigating the potential role of aspartame in colorectal cancer by suggesting two experimental approaches: (i) an in vitro cytotoxicity screening in HT-29 human colorectal carcinoma cells based on cell viability (Alamar blue assay), cell morphology and cell migration (scratch assay) assessment and (ii) an in ovo evaluation in terms of angiogenic and irritant potential by means of the chorioallantoic membrane method (CAM). The in vitro results showed a dose-dependent cytotoxic effect, with a significant decrease of viable cells at the highest concentrations tested (15, 30 and 50 mM) and morphological cellular changes. In ovo, aspartame (15 and 30 mM) proved to have a pro-angiogenic effect and a weak irritant potential at the vascular level. These data suggest new directions of research regarding aspartame's role in colorectal cancer. |
| doi_str_mv | 10.3390/nu12123600 |
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This study aims at investigating the potential role of aspartame in colorectal cancer by suggesting two experimental approaches: (i) an in vitro cytotoxicity screening in HT-29 human colorectal carcinoma cells based on cell viability (Alamar blue assay), cell morphology and cell migration (scratch assay) assessment and (ii) an in ovo evaluation in terms of angiogenic and irritant potential by means of the chorioallantoic membrane method (CAM). The in vitro results showed a dose-dependent cytotoxic effect, with a significant decrease of viable cells at the highest concentrations tested (15, 30 and 50 mM) and morphological cellular changes. In ovo, aspartame (15 and 30 mM) proved to have a pro-angiogenic effect and a weak irritant potential at the vascular level. These data suggest new directions of research regarding aspartame's role in colorectal cancer.</description><identifier>ISSN: 2072-6643</identifier><identifier>EISSN: 2072-6643</identifier><identifier>DOI: 10.3390/nu12123600</identifier><identifier>PMID: 33255204</identifier><language>eng</language><publisher>BASEL: Mdpi</publisher><subject>Angiogenesis ; Angiogenesis Inducing Agents - metabolism ; Angiogenesis Inducing Agents - pharmacology ; Artificial sweeteners ; Aspartame ; Aspartame - metabolism ; Aspartame - pharmacology ; Carcinogenesis ; Carcinogens ; Cell adhesion & migration ; Cell culture ; Cell morphology ; Cell Survival - drug effects ; Cell viability ; Chorioallantoic membrane ; Colorectal cancer ; Colorectal carcinoma ; Colorectal Neoplasms - metabolism ; Cytology ; Cytotoxicity ; Cytotoxins - metabolism ; Cytotoxins - pharmacology ; Diet ; Health risks ; HT29 Cells - metabolism ; Humans ; In vitro methods and tests ; In Vitro Techniques ; Life Sciences & Biomedicine ; Morphology ; Nutrition & Dietetics ; Penicillin ; Science & Technology ; Software ; Sugar ; Sweetening Agents - metabolism ; Sweetening Agents - pharmacology</subject><ispartof>Nutrients, 2020-11, Vol.12 (12), p.3600, Article 3600</ispartof><rights>2020. This work is licensed under http://creativecommons.org/licenses/by/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2020 by the authors. 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>true</woscitedreferencessubscribed><woscitedreferencescount>27</woscitedreferencescount><woscitedreferencesoriginalsourcerecordid>wos000602369100001</woscitedreferencesoriginalsourcerecordid><citedby>FETCH-LOGICAL-c406t-30fdd29e48cb8008f4084e8d75ffbf15247c96e9a292e9815c1d6133d821ec3e3</citedby><cites>FETCH-LOGICAL-c406t-30fdd29e48cb8008f4084e8d75ffbf15247c96e9a292e9815c1d6133d821ec3e3</cites><orcidid>0000-0003-3292-9079 ; 0000-0002-5760-8872 ; 0009-0002-8083-4509 ; 0000-0003-2295-1926</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7760274/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7760274/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,886,27929,27930,28253,53796,53798</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33255204$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Maghiari, Anca Laura</creatorcontrib><creatorcontrib>Coricovac, Dorina</creatorcontrib><creatorcontrib>Pinzaru, Iulia Andreea</creatorcontrib><creatorcontrib>Macasoi, Ioana Gabriela</creatorcontrib><creatorcontrib>Marcovici, Iasmina</creatorcontrib><creatorcontrib>Simu, Sebastian</creatorcontrib><creatorcontrib>Navolan, Dan</creatorcontrib><creatorcontrib>Dehelean, Cristina</creatorcontrib><title>High Concentrations of Aspartame Induce Pro-Angiogenic Effects in Ovo and Cytotoxic Effects in HT-29 Human Colorectal Carcinoma Cells</title><title>Nutrients</title><addtitle>NUTRIENTS</addtitle><addtitle>Nutrients</addtitle><description>Aspartame (ASP), an artificial sweetener abundantly consumed in recent years in an array of dietary products, has raised some concerns in terms of toxicity, and it was even suggested a link with the risk of carcinogenesis (colorectal cancer), though the present scientific data are rather inconclusive. This study aims at investigating the potential role of aspartame in colorectal cancer by suggesting two experimental approaches: (i) an in vitro cytotoxicity screening in HT-29 human colorectal carcinoma cells based on cell viability (Alamar blue assay), cell morphology and cell migration (scratch assay) assessment and (ii) an in ovo evaluation in terms of angiogenic and irritant potential by means of the chorioallantoic membrane method (CAM). The in vitro results showed a dose-dependent cytotoxic effect, with a significant decrease of viable cells at the highest concentrations tested (15, 30 and 50 mM) and morphological cellular changes. In ovo, aspartame (15 and 30 mM) proved to have a pro-angiogenic effect and a weak irritant potential at the vascular level. 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This study aims at investigating the potential role of aspartame in colorectal cancer by suggesting two experimental approaches: (i) an in vitro cytotoxicity screening in HT-29 human colorectal carcinoma cells based on cell viability (Alamar blue assay), cell morphology and cell migration (scratch assay) assessment and (ii) an in ovo evaluation in terms of angiogenic and irritant potential by means of the chorioallantoic membrane method (CAM). The in vitro results showed a dose-dependent cytotoxic effect, with a significant decrease of viable cells at the highest concentrations tested (15, 30 and 50 mM) and morphological cellular changes. In ovo, aspartame (15 and 30 mM) proved to have a pro-angiogenic effect and a weak irritant potential at the vascular level. 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| subjects | Angiogenesis Angiogenesis Inducing Agents - metabolism Angiogenesis Inducing Agents - pharmacology Artificial sweeteners Aspartame Aspartame - metabolism Aspartame - pharmacology Carcinogenesis Carcinogens Cell adhesion & migration Cell culture Cell morphology Cell Survival - drug effects Cell viability Chorioallantoic membrane Colorectal cancer Colorectal carcinoma Colorectal Neoplasms - metabolism Cytology Cytotoxicity Cytotoxins - metabolism Cytotoxins - pharmacology Diet Health risks HT29 Cells - metabolism Humans In vitro methods and tests In Vitro Techniques Life Sciences & Biomedicine Morphology Nutrition & Dietetics Penicillin Science & Technology Software Sugar Sweetening Agents - metabolism Sweetening Agents - pharmacology |
| title | High Concentrations of Aspartame Induce Pro-Angiogenic Effects in Ovo and Cytotoxic Effects in HT-29 Human Colorectal Carcinoma Cells |
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