Glucose-Uptake Activity and Cytotoxicity of Diterpenes and Triterpenes Isolated from Lamiaceae Plant Species
The prevalence of diabetes mellitus (DM), considered one of the most common metabolic disorders, has dramatically increased and resulted in higher rates of morbidity and mortality around the world in the past decade. It is well known that insulin resistance in target tissues and a deficiency in insu...
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| Veröffentlicht in: | Molecules (Basel, Switzerland) Switzerland), 2020-09, Vol.25 (18), p.4129, Article 4129 |
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| creator | Etsassala, Ninon G. E. R. Ndjoubi, Kadidiatou O. Mbira, Thilly J. Pearce, Brendon Pearce, Keenau Iwuoha, Emmanuel I. Hussein, Ahmed A. Benjeddou, Mongi |
| description | The prevalence of diabetes mellitus (DM), considered one of the most common metabolic disorders, has dramatically increased and resulted in higher rates of morbidity and mortality around the world in the past decade. It is well known that insulin resistance in target tissues and a deficiency in insulin secretion from pancreatic beta-cells are the main characteristics of type 2 diabetes. The aim of this study was the bio-evaluation of compounds isolated from three selected plant species: namely,Salvia africana-lutea,Leonotis ocymifolia, andPlectranthus madagascariensis, for their glucose-uptake ability. Methanolic extracts were produced from the aerial parts of each plant. Compounds were identified using different spectroscopic techniques. The glucose-uptake ability of each compound was then evaluated in mammalian cells using 2-deoxyglucose-6-phosphate. The cytotoxicity of each compound was established via the MTT assay. Chromatographic purification of the three plant species yielded sixteen pure terpenoids. Compounds1(p= 0.0031),8(p= 0.0053), and6(p= 0.0086) showed a marked increase in glucose uptake, respectively. Additionally,1,4, and6exhibited cytotoxicity toward mammalian tissue with a decrease in cell viability of similar to 70%, similar to 68%, and similar to 67%, respectively. The results suggested that several compounds demonstrated a marked increase in glucose uptake, while two of the compounds exhibited signs of cytotoxicity. It may, therefore, be suggested that these compounds be considered as potential candidates for novel plant-derived alternative therapies in the treatment of type 2 diabetes. |
| doi_str_mv | 10.3390/molecules25184129 |
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E. R. ; Ndjoubi, Kadidiatou O. ; Mbira, Thilly J. ; Pearce, Brendon ; Pearce, Keenau ; Iwuoha, Emmanuel I. ; Hussein, Ahmed A. ; Benjeddou, Mongi</creator><creatorcontrib>Etsassala, Ninon G. E. R. ; Ndjoubi, Kadidiatou O. ; Mbira, Thilly J. ; Pearce, Brendon ; Pearce, Keenau ; Iwuoha, Emmanuel I. ; Hussein, Ahmed A. ; Benjeddou, Mongi</creatorcontrib><description>The prevalence of diabetes mellitus (DM), considered one of the most common metabolic disorders, has dramatically increased and resulted in higher rates of morbidity and mortality around the world in the past decade. It is well known that insulin resistance in target tissues and a deficiency in insulin secretion from pancreatic beta-cells are the main characteristics of type 2 diabetes. The aim of this study was the bio-evaluation of compounds isolated from three selected plant species: namely,Salvia africana-lutea,Leonotis ocymifolia, andPlectranthus madagascariensis, for their glucose-uptake ability. Methanolic extracts were produced from the aerial parts of each plant. Compounds were identified using different spectroscopic techniques. The glucose-uptake ability of each compound was then evaluated in mammalian cells using 2-deoxyglucose-6-phosphate. The cytotoxicity of each compound was established via the MTT assay. Chromatographic purification of the three plant species yielded sixteen pure terpenoids. Compounds1(p= 0.0031),8(p= 0.0053), and6(p= 0.0086) showed a marked increase in glucose uptake, respectively. Additionally,1,4, and6exhibited cytotoxicity toward mammalian tissue with a decrease in cell viability of similar to 70%, similar to 68%, and similar to 67%, respectively. The results suggested that several compounds demonstrated a marked increase in glucose uptake, while two of the compounds exhibited signs of cytotoxicity. It may, therefore, be suggested that these compounds be considered as potential candidates for novel plant-derived alternative therapies in the treatment of type 2 diabetes.</description><identifier>ISSN: 1420-3049</identifier><identifier>EISSN: 1420-3049</identifier><identifier>DOI: 10.3390/molecules25184129</identifier><identifier>PMID: 32927596</identifier><language>eng</language><publisher>BASEL: Mdpi</publisher><subject><![CDATA[abietane diterpenes ; Acids ; Adipocytes ; Beta cells ; Biochemistry & Molecular Biology ; Carbohydrate Metabolism - drug effects ; Cell Line ; Cell Survival - drug effects ; Cell viability ; Chemistry ; Chemistry, Multidisciplinary ; Chromatography ; Cytotoxicity ; Deoxyglucose ; Diabetes ; Diabetes mellitus ; Diabetes mellitus (non-insulin dependent) ; Diterpenes ; Diterpenes - chemistry ; Diterpenes - isolation & purification ; Diterpenes - pharmacology ; Flowers & plants ; Glucose ; Glucose - metabolism ; glucose uptake ; Humans ; Insulin ; Insulin resistance ; Insulin secretion ; Lamiaceae ; Lamiaceae - chemistry ; Life Sciences & Biomedicine ; Mammalian cells ; Mammals ; Metabolic disorders ; Molecular Structure ; Morbidity ; Pancreas ; Physical Sciences ; Phytochemicals ; Plant Extracts - chemistry ; Plant Extracts - isolation & purification ; Plant Extracts - pharmacology ; Plant species ; Plants ; Science & Technology ; terpenoids ; Triterpenes ; Triterpenes - chemistry ; Triterpenes - isolation & purification ; Triterpenes - pharmacology ; ursolic acid]]></subject><ispartof>Molecules (Basel, Switzerland), 2020-09, Vol.25 (18), p.4129, Article 4129</ispartof><rights>2020. This work is licensed under http://creativecommons.org/licenses/by/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2020 by the authors. 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>true</woscitedreferencessubscribed><woscitedreferencescount>8</woscitedreferencescount><woscitedreferencesoriginalsourcerecordid>wos000580205400001</woscitedreferencesoriginalsourcerecordid><citedby>FETCH-LOGICAL-c493t-dd48326369e8b86983ecb067bb8531bfe8be6eb37c520f77aeea94c39943b5033</citedby><cites>FETCH-LOGICAL-c493t-dd48326369e8b86983ecb067bb8531bfe8be6eb37c520f77aeea94c39943b5033</cites><orcidid>0000-0002-6531-4102 ; 0000-0002-0569-4078 ; 0000-0002-0124-319X ; 0000-0002-3877-9959 ; 0000-0002-7501-5771 ; 0000-0001-6102-0433</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7570927/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7570927/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,865,886,2103,2115,27929,27930,28253,53796,53798</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32927596$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Etsassala, Ninon G. E. R.</creatorcontrib><creatorcontrib>Ndjoubi, Kadidiatou O.</creatorcontrib><creatorcontrib>Mbira, Thilly J.</creatorcontrib><creatorcontrib>Pearce, Brendon</creatorcontrib><creatorcontrib>Pearce, Keenau</creatorcontrib><creatorcontrib>Iwuoha, Emmanuel I.</creatorcontrib><creatorcontrib>Hussein, Ahmed A.</creatorcontrib><creatorcontrib>Benjeddou, Mongi</creatorcontrib><title>Glucose-Uptake Activity and Cytotoxicity of Diterpenes and Triterpenes Isolated from Lamiaceae Plant Species</title><title>Molecules (Basel, Switzerland)</title><addtitle>MOLECULES</addtitle><addtitle>Molecules</addtitle><description>The prevalence of diabetes mellitus (DM), considered one of the most common metabolic disorders, has dramatically increased and resulted in higher rates of morbidity and mortality around the world in the past decade. It is well known that insulin resistance in target tissues and a deficiency in insulin secretion from pancreatic beta-cells are the main characteristics of type 2 diabetes. The aim of this study was the bio-evaluation of compounds isolated from three selected plant species: namely,Salvia africana-lutea,Leonotis ocymifolia, andPlectranthus madagascariensis, for their glucose-uptake ability. Methanolic extracts were produced from the aerial parts of each plant. Compounds were identified using different spectroscopic techniques. The glucose-uptake ability of each compound was then evaluated in mammalian cells using 2-deoxyglucose-6-phosphate. The cytotoxicity of each compound was established via the MTT assay. Chromatographic purification of the three plant species yielded sixteen pure terpenoids. Compounds1(p= 0.0031),8(p= 0.0053), and6(p= 0.0086) showed a marked increase in glucose uptake, respectively. Additionally,1,4, and6exhibited cytotoxicity toward mammalian tissue with a decrease in cell viability of similar to 70%, similar to 68%, and similar to 67%, respectively. The results suggested that several compounds demonstrated a marked increase in glucose uptake, while two of the compounds exhibited signs of cytotoxicity. It may, therefore, be suggested that these compounds be considered as potential candidates for novel plant-derived alternative therapies in the treatment of type 2 diabetes.</description><subject>abietane diterpenes</subject><subject>Acids</subject><subject>Adipocytes</subject><subject>Beta cells</subject><subject>Biochemistry & Molecular Biology</subject><subject>Carbohydrate Metabolism - drug effects</subject><subject>Cell Line</subject><subject>Cell Survival - drug effects</subject><subject>Cell viability</subject><subject>Chemistry</subject><subject>Chemistry, Multidisciplinary</subject><subject>Chromatography</subject><subject>Cytotoxicity</subject><subject>Deoxyglucose</subject><subject>Diabetes</subject><subject>Diabetes mellitus</subject><subject>Diabetes mellitus (non-insulin dependent)</subject><subject>Diterpenes</subject><subject>Diterpenes - chemistry</subject><subject>Diterpenes - isolation & purification</subject><subject>Diterpenes - pharmacology</subject><subject>Flowers & plants</subject><subject>Glucose</subject><subject>Glucose - metabolism</subject><subject>glucose uptake</subject><subject>Humans</subject><subject>Insulin</subject><subject>Insulin resistance</subject><subject>Insulin secretion</subject><subject>Lamiaceae</subject><subject>Lamiaceae - chemistry</subject><subject>Life Sciences & Biomedicine</subject><subject>Mammalian cells</subject><subject>Mammals</subject><subject>Metabolic disorders</subject><subject>Molecular Structure</subject><subject>Morbidity</subject><subject>Pancreas</subject><subject>Physical Sciences</subject><subject>Phytochemicals</subject><subject>Plant Extracts - chemistry</subject><subject>Plant Extracts - isolation & purification</subject><subject>Plant Extracts - pharmacology</subject><subject>Plant species</subject><subject>Plants</subject><subject>Science & Technology</subject><subject>terpenoids</subject><subject>Triterpenes</subject><subject>Triterpenes - chemistry</subject><subject>Triterpenes - isolation & purification</subject><subject>Triterpenes - pharmacology</subject><subject>ursolic acid</subject><issn>1420-3049</issn><issn>1420-3049</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>AOWDO</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>DOA</sourceid><recordid>eNqNkk1v1DAQhiMEoh_wA7igSFwqVQF_xbEvSFWAstJKINGeLduZFC9JvNhOYf893t2ytHDh5PE7z7waj6coXmD0mlKJ3ox-ADsPEEmNBcNEPiqOMSOooojJx_fio-IkxhVCBDNcPy2OKJGkqSU_LobLYbY-QnW9TvoblBc2uVuXNqWeurLdJJ_8T2e3gu_Ldy5BWMMEcZe-Cn_ui-gHnaAr--DHcqlHpy1oKD8PekrllzVYB_FZ8aTXQ4Tnd-dpcf3h_VX7sVp-uly0F8vKMklT1XVMUMIplyCM4FJQsAbxxhhRU2z6rAIHQxtbE9Q3jQbQklkqJaOmRpSeFou9b-f1Sq2DG3XYKK-d2gk-3CgdkrMDKDAGd73oBRDEDAepBZOiM1hwInG39Xq791rPZoTOwpSCHh6YPsxM7qu68beqqRuUp5wNzu4Mgv8-Q0xqdNHCkAcDfo6KMEYEo4LIjL76C135OUx5VDuKS044yRTeUzb4GAP0h2YwUtu9UP_sRa55ef8Vh4rfi5CB8z3wA4zvY_6tycIBQwjVAhFUsxwhnGnx_3Trkk7OT62fp0R_AWkj2HM</recordid><startdate>20200910</startdate><enddate>20200910</enddate><creator>Etsassala, Ninon G. 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E. R. ; Ndjoubi, Kadidiatou O. ; Mbira, Thilly J. ; Pearce, Brendon ; Pearce, Keenau ; Iwuoha, Emmanuel I. ; Hussein, Ahmed A. ; Benjeddou, Mongi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c493t-dd48326369e8b86983ecb067bb8531bfe8be6eb37c520f77aeea94c39943b5033</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>abietane diterpenes</topic><topic>Acids</topic><topic>Adipocytes</topic><topic>Beta cells</topic><topic>Biochemistry & Molecular Biology</topic><topic>Carbohydrate Metabolism - drug effects</topic><topic>Cell Line</topic><topic>Cell Survival - drug effects</topic><topic>Cell viability</topic><topic>Chemistry</topic><topic>Chemistry, Multidisciplinary</topic><topic>Chromatography</topic><topic>Cytotoxicity</topic><topic>Deoxyglucose</topic><topic>Diabetes</topic><topic>Diabetes mellitus</topic><topic>Diabetes mellitus (non-insulin dependent)</topic><topic>Diterpenes</topic><topic>Diterpenes - chemistry</topic><topic>Diterpenes - isolation & purification</topic><topic>Diterpenes - pharmacology</topic><topic>Flowers & plants</topic><topic>Glucose</topic><topic>Glucose - metabolism</topic><topic>glucose uptake</topic><topic>Humans</topic><topic>Insulin</topic><topic>Insulin resistance</topic><topic>Insulin secretion</topic><topic>Lamiaceae</topic><topic>Lamiaceae - chemistry</topic><topic>Life Sciences & Biomedicine</topic><topic>Mammalian cells</topic><topic>Mammals</topic><topic>Metabolic disorders</topic><topic>Molecular Structure</topic><topic>Morbidity</topic><topic>Pancreas</topic><topic>Physical Sciences</topic><topic>Phytochemicals</topic><topic>Plant Extracts - chemistry</topic><topic>Plant Extracts - isolation & purification</topic><topic>Plant Extracts - pharmacology</topic><topic>Plant species</topic><topic>Plants</topic><topic>Science & Technology</topic><topic>terpenoids</topic><topic>Triterpenes</topic><topic>Triterpenes - chemistry</topic><topic>Triterpenes - isolation & purification</topic><topic>Triterpenes - pharmacology</topic><topic>ursolic acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Etsassala, Ninon G. 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E. R.</au><au>Ndjoubi, Kadidiatou O.</au><au>Mbira, Thilly J.</au><au>Pearce, Brendon</au><au>Pearce, Keenau</au><au>Iwuoha, Emmanuel I.</au><au>Hussein, Ahmed A.</au><au>Benjeddou, Mongi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Glucose-Uptake Activity and Cytotoxicity of Diterpenes and Triterpenes Isolated from Lamiaceae Plant Species</atitle><jtitle>Molecules (Basel, Switzerland)</jtitle><stitle>MOLECULES</stitle><addtitle>Molecules</addtitle><date>2020-09-10</date><risdate>2020</risdate><volume>25</volume><issue>18</issue><spage>4129</spage><pages>4129-</pages><artnum>4129</artnum><issn>1420-3049</issn><eissn>1420-3049</eissn><abstract>The prevalence of diabetes mellitus (DM), considered one of the most common metabolic disorders, has dramatically increased and resulted in higher rates of morbidity and mortality around the world in the past decade. It is well known that insulin resistance in target tissues and a deficiency in insulin secretion from pancreatic beta-cells are the main characteristics of type 2 diabetes. The aim of this study was the bio-evaluation of compounds isolated from three selected plant species: namely,Salvia africana-lutea,Leonotis ocymifolia, andPlectranthus madagascariensis, for their glucose-uptake ability. Methanolic extracts were produced from the aerial parts of each plant. Compounds were identified using different spectroscopic techniques. The glucose-uptake ability of each compound was then evaluated in mammalian cells using 2-deoxyglucose-6-phosphate. The cytotoxicity of each compound was established via the MTT assay. Chromatographic purification of the three plant species yielded sixteen pure terpenoids. Compounds1(p= 0.0031),8(p= 0.0053), and6(p= 0.0086) showed a marked increase in glucose uptake, respectively. 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| subjects | abietane diterpenes Acids Adipocytes Beta cells Biochemistry & Molecular Biology Carbohydrate Metabolism - drug effects Cell Line Cell Survival - drug effects Cell viability Chemistry Chemistry, Multidisciplinary Chromatography Cytotoxicity Deoxyglucose Diabetes Diabetes mellitus Diabetes mellitus (non-insulin dependent) Diterpenes Diterpenes - chemistry Diterpenes - isolation & purification Diterpenes - pharmacology Flowers & plants Glucose Glucose - metabolism glucose uptake Humans Insulin Insulin resistance Insulin secretion Lamiaceae Lamiaceae - chemistry Life Sciences & Biomedicine Mammalian cells Mammals Metabolic disorders Molecular Structure Morbidity Pancreas Physical Sciences Phytochemicals Plant Extracts - chemistry Plant Extracts - isolation & purification Plant Extracts - pharmacology Plant species Plants Science & Technology terpenoids Triterpenes Triterpenes - chemistry Triterpenes - isolation & purification Triterpenes - pharmacology ursolic acid |
| title | Glucose-Uptake Activity and Cytotoxicity of Diterpenes and Triterpenes Isolated from Lamiaceae Plant Species |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-14T05%3A25%3A04IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Glucose-Uptake%20Activity%20and%20Cytotoxicity%20of%20Diterpenes%20and%20Triterpenes%20Isolated%20from%20Lamiaceae%20Plant%20Species&rft.jtitle=Molecules%20(Basel,%20Switzerland)&rft.au=Etsassala,%20Ninon%20G.%20E.%20R.&rft.date=2020-09-10&rft.volume=25&rft.issue=18&rft.spage=4129&rft.pages=4129-&rft.artnum=4129&rft.issn=1420-3049&rft.eissn=1420-3049&rft_id=info:doi/10.3390/molecules25184129&rft_dat=%3Cproquest_pubme%3E2442696262%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2442696262&rft_id=info:pmid/32927596&rft_doaj_id=oai_doaj_org_article_ebb1df8f8e204b6e9a8498db186291d3&rfr_iscdi=true |