Antagonistic effects of nano-selenium on broilers hepatic injury induced by Cr(VI) poisoning in AMPK pathway
Cr (chromium, with common valence states of III and VI) is one of the common broiler feed additives. Liver injury and metabolic disorders could be caused by Cr (VI) (hexavalent chromium) poisoning in broilers. Oxidative damage and metabolic disorders of organisms caused by heavy metals could be anta...
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| creator | Zhang, Tian-guang Zhao, Ya-li Li, Lei Zhou, Dong-hai |
| description | Cr (chromium, with common valence states of III and VI) is one of the common broiler feed additives. Liver injury and metabolic disorders could be caused by Cr
(VI)
(hexavalent chromium) poisoning in broilers. Oxidative damage and metabolic disorders of organisms caused by heavy metals could be antagonized by nano-Se (nano-selenium). Nano-Se was chosen to study the antagonism of Cr
(VI)
poisoning in broilers. AMPK (Adenosine 5
,
-monophosphate-activated protein kinase) is known as a “cell energy regulator” and plays a key regulatory role in carbohydrate and lipid metabolism. AMPK pathway and
ACACA
/
CPT1A
two genes were selected to study the prevention and treatment of nano-Se on Cr
(VI)
poisoning in broilers and its molecular mechanism. For this purpose, 180 1-day-old AA (Arbor Acres) broilers were selected and randomly divided into 6 groups (
n
= 30) for further testing. After feeding as planned for 35 days, the livers of such broilers were taken for further examination including histopathological examination, differential gene expression analysis, and further validation on both mRNA and protein levels using related techniques like RT-qPCR, western blot, and immunohistochemistry (IHC). The histopathological examination suggested that the liver cells of the Cr
(VI)
poisoning group were more severely injured than the nano-Se addition group. RT-qPCR results showed that the relative expression of
ACACA
gene in the Cr
(VI)
poisoning group was significantly increased (
P
|
| doi_str_mv | 10.1007/s11356-020-08501-0 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmed_primary_32691314</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2471534334</sourcerecordid><originalsourceid>FETCH-LOGICAL-c412t-cd3ca1f39f6f704ddc88947d4d36e0dab60ae7a9da6c648e5474c364f57bb4d13</originalsourceid><addsrcrecordid>eNqNkF1rFDEUhoModl39A15IwJtKiZ5MziQzl8vgR7GiF-rtkMnHdpbdZE1mKPvvTZ223okQOIE875vkIeQlh7ccQL3LnItaMqiAQVMDZ_CIrLjkyBS27WOyghaRcYF4Rp7lvINCtpV6Ss5EJVsuOK7IfhMmvY1hzNNoqPPemSnT6GnQIbLs9i6M84HGQIcUx71LmV67o76Fx7Cb06kMOxtn6XCiXTr_efmGHuOYS2PYljO6-fLtMy2B6xt9ek6eeL3P7sXdXJMfH95_7z6xq68fL7vNFTPIq4kZK4zmXrReegVorWmaFpVFK6QDqwcJ2indWi2NxMbVqNAIib5Ww4CWizV5vfQeU_w1uzz1uzinUK7sK1S8FijKWpNqoUyKOSfn-2MaDzqdeg79reB-EdwXbf0fwT2U0Ku76nk4OPsQuTdagGYBbtwQfTajC8Y9YABQ1yARmrKDqhunojKGLs5hKtGL_48WWix0LkTYuvT3k_94_29Yg6c2</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2471534334</pqid></control><display><type>article</type><title>Antagonistic effects of nano-selenium on broilers hepatic injury induced by Cr(VI) poisoning in AMPK pathway</title><source>MEDLINE</source><source>Web of Science - Science Citation Index Expanded - 2020<img src="https://exlibris-pub.s3.amazonaws.com/fromwos-v2.jpg" /></source><source>SpringerLink Journals - AutoHoldings</source><creator>Zhang, Tian-guang ; Zhao, Ya-li ; Li, Lei ; Zhou, Dong-hai</creator><creatorcontrib>Zhang, Tian-guang ; Zhao, Ya-li ; Li, Lei ; Zhou, Dong-hai</creatorcontrib><description>Cr (chromium, with common valence states of III and VI) is one of the common broiler feed additives. Liver injury and metabolic disorders could be caused by Cr
(VI)
(hexavalent chromium) poisoning in broilers. Oxidative damage and metabolic disorders of organisms caused by heavy metals could be antagonized by nano-Se (nano-selenium). Nano-Se was chosen to study the antagonism of Cr
(VI)
poisoning in broilers. AMPK (Adenosine 5
,
-monophosphate-activated protein kinase) is known as a “cell energy regulator” and plays a key regulatory role in carbohydrate and lipid metabolism. AMPK pathway and
ACACA
/
CPT1A
two genes were selected to study the prevention and treatment of nano-Se on Cr
(VI)
poisoning in broilers and its molecular mechanism. For this purpose, 180 1-day-old AA (Arbor Acres) broilers were selected and randomly divided into 6 groups (
n
= 30) for further testing. After feeding as planned for 35 days, the livers of such broilers were taken for further examination including histopathological examination, differential gene expression analysis, and further validation on both mRNA and protein levels using related techniques like RT-qPCR, western blot, and immunohistochemistry (IHC). The histopathological examination suggested that the liver cells of the Cr
(VI)
poisoning group were more severely injured than the nano-Se addition group. RT-qPCR results showed that the relative expression of
ACACA
gene in the Cr
(VI)
poisoning group was significantly increased (
P
< 0.05), while the
CPT1A
gene’s expression was significantly decreased (
P
< 0.01). Those results were reversed in the nano-Se addition group. Western blot results were consistent with RT-qPCR and both suggested antagonism of nano-Se on Cr
(VI)
. Through morphological and histopathological observation, as well as the measurement of the mRNA and protein expression levels of
ACACA
and
CPT1A
genes in AMPK pathway, it was confirmed that nano-Se has certain preventive and protective effects on Cr
(VI)
poisoning in broiler chickens. Furthermore, the adverse effects of Cr
(VI)
on carbohydrate and lipid metabolism in broilers can be antagonized by nano-Se through AMPK pathway. A new method and experimental basis were provided to the future study of Cr
(VI)
poisoning in broilers.</description><identifier>ISSN: 0944-1344</identifier><identifier>EISSN: 1614-7499</identifier><identifier>DOI: 10.1007/s11356-020-08501-0</identifier><identifier>PMID: 32691314</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Adenosine kinase ; Adenosine monophosphate ; AMP ; AMP-Activated Protein Kinases - genetics ; AMP-Activated Protein Kinases - metabolism ; Animals ; Aquatic Pollution ; Atmospheric Protection/Air Quality Control/Air Pollution ; Carbohydrate metabolism ; Carbohydrates ; Chickens ; Chromium ; Chromium - metabolism ; Chromium - toxicity ; Earth and Environmental Science ; Ecotoxicology ; Environment ; Environmental Chemistry ; Environmental Health ; Environmental science ; Environmental Sciences ; Environmental Sciences & Ecology ; Feed additives ; Food additives ; Gene expression ; Genes ; Heavy metals ; Hepatocytes ; Hexavalent chromium ; Immunohistochemistry ; Injuries ; Kinases ; Life Sciences & Biomedicine ; Lipid Metabolism ; Lipids ; Liver ; Liver - metabolism ; Liver diseases ; Metabolic disorders ; Poisoning ; Poultry ; Poultry feed ; Protein kinase ; Proteins ; Research Article ; Science & Technology ; Selenium ; Selenium - metabolism ; Valence ; Waste Water Technology ; Water Management ; Water Pollution Control</subject><ispartof>Environmental science and pollution research international, 2020-11, Vol.27 (33), p.41585-41595</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2020</rights><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>true</woscitedreferencessubscribed><woscitedreferencescount>18</woscitedreferencescount><woscitedreferencesoriginalsourcerecordid>wos000550640800002</woscitedreferencesoriginalsourcerecordid><citedby>FETCH-LOGICAL-c412t-cd3ca1f39f6f704ddc88947d4d36e0dab60ae7a9da6c648e5474c364f57bb4d13</citedby><cites>FETCH-LOGICAL-c412t-cd3ca1f39f6f704ddc88947d4d36e0dab60ae7a9da6c648e5474c364f57bb4d13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11356-020-08501-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11356-020-08501-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>315,781,785,27929,27930,28253,41493,42562,51324</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32691314$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Tian-guang</creatorcontrib><creatorcontrib>Zhao, Ya-li</creatorcontrib><creatorcontrib>Li, Lei</creatorcontrib><creatorcontrib>Zhou, Dong-hai</creatorcontrib><title>Antagonistic effects of nano-selenium on broilers hepatic injury induced by Cr(VI) poisoning in AMPK pathway</title><title>Environmental science and pollution research international</title><addtitle>Environ Sci Pollut Res</addtitle><addtitle>ENVIRON SCI POLLUT R</addtitle><addtitle>Environ Sci Pollut Res Int</addtitle><description>Cr (chromium, with common valence states of III and VI) is one of the common broiler feed additives. Liver injury and metabolic disorders could be caused by Cr
(VI)
(hexavalent chromium) poisoning in broilers. Oxidative damage and metabolic disorders of organisms caused by heavy metals could be antagonized by nano-Se (nano-selenium). Nano-Se was chosen to study the antagonism of Cr
(VI)
poisoning in broilers. AMPK (Adenosine 5
,
-monophosphate-activated protein kinase) is known as a “cell energy regulator” and plays a key regulatory role in carbohydrate and lipid metabolism. AMPK pathway and
ACACA
/
CPT1A
two genes were selected to study the prevention and treatment of nano-Se on Cr
(VI)
poisoning in broilers and its molecular mechanism. For this purpose, 180 1-day-old AA (Arbor Acres) broilers were selected and randomly divided into 6 groups (
n
= 30) for further testing. After feeding as planned for 35 days, the livers of such broilers were taken for further examination including histopathological examination, differential gene expression analysis, and further validation on both mRNA and protein levels using related techniques like RT-qPCR, western blot, and immunohistochemistry (IHC). The histopathological examination suggested that the liver cells of the Cr
(VI)
poisoning group were more severely injured than the nano-Se addition group. RT-qPCR results showed that the relative expression of
ACACA
gene in the Cr
(VI)
poisoning group was significantly increased (
P
< 0.05), while the
CPT1A
gene’s expression was significantly decreased (
P
< 0.01). Those results were reversed in the nano-Se addition group. Western blot results were consistent with RT-qPCR and both suggested antagonism of nano-Se on Cr
(VI)
. Through morphological and histopathological observation, as well as the measurement of the mRNA and protein expression levels of
ACACA
and
CPT1A
genes in AMPK pathway, it was confirmed that nano-Se has certain preventive and protective effects on Cr
(VI)
poisoning in broiler chickens. Furthermore, the adverse effects of Cr
(VI)
on carbohydrate and lipid metabolism in broilers can be antagonized by nano-Se through AMPK pathway. A new method and experimental basis were provided to the future study of Cr
(VI)
poisoning in broilers.</description><subject>Adenosine kinase</subject><subject>Adenosine monophosphate</subject><subject>AMP</subject><subject>AMP-Activated Protein Kinases - genetics</subject><subject>AMP-Activated Protein Kinases - metabolism</subject><subject>Animals</subject><subject>Aquatic Pollution</subject><subject>Atmospheric Protection/Air Quality Control/Air Pollution</subject><subject>Carbohydrate metabolism</subject><subject>Carbohydrates</subject><subject>Chickens</subject><subject>Chromium</subject><subject>Chromium - metabolism</subject><subject>Chromium - toxicity</subject><subject>Earth and Environmental Science</subject><subject>Ecotoxicology</subject><subject>Environment</subject><subject>Environmental Chemistry</subject><subject>Environmental Health</subject><subject>Environmental science</subject><subject>Environmental Sciences</subject><subject>Environmental Sciences & Ecology</subject><subject>Feed additives</subject><subject>Food additives</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Heavy metals</subject><subject>Hepatocytes</subject><subject>Hexavalent chromium</subject><subject>Immunohistochemistry</subject><subject>Injuries</subject><subject>Kinases</subject><subject>Life Sciences & Biomedicine</subject><subject>Lipid Metabolism</subject><subject>Lipids</subject><subject>Liver</subject><subject>Liver - metabolism</subject><subject>Liver diseases</subject><subject>Metabolic disorders</subject><subject>Poisoning</subject><subject>Poultry</subject><subject>Poultry feed</subject><subject>Protein kinase</subject><subject>Proteins</subject><subject>Research Article</subject><subject>Science & Technology</subject><subject>Selenium</subject><subject>Selenium - metabolism</subject><subject>Valence</subject><subject>Waste Water Technology</subject><subject>Water Management</subject><subject>Water Pollution Control</subject><issn>0944-1344</issn><issn>1614-7499</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>AOWDO</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkF1rFDEUhoModl39A15IwJtKiZ5MziQzl8vgR7GiF-rtkMnHdpbdZE1mKPvvTZ223okQOIE875vkIeQlh7ccQL3LnItaMqiAQVMDZ_CIrLjkyBS27WOyghaRcYF4Rp7lvINCtpV6Ss5EJVsuOK7IfhMmvY1hzNNoqPPemSnT6GnQIbLs9i6M84HGQIcUx71LmV67o76Fx7Cb06kMOxtn6XCiXTr_efmGHuOYS2PYljO6-fLtMy2B6xt9ek6eeL3P7sXdXJMfH95_7z6xq68fL7vNFTPIq4kZK4zmXrReegVorWmaFpVFK6QDqwcJ2indWi2NxMbVqNAIib5Ww4CWizV5vfQeU_w1uzz1uzinUK7sK1S8FijKWpNqoUyKOSfn-2MaDzqdeg79reB-EdwXbf0fwT2U0Ku76nk4OPsQuTdagGYBbtwQfTajC8Y9YABQ1yARmrKDqhunojKGLs5hKtGL_48WWix0LkTYuvT3k_94_29Yg6c2</recordid><startdate>20201101</startdate><enddate>20201101</enddate><creator>Zhang, Tian-guang</creator><creator>Zhao, Ya-li</creator><creator>Li, Lei</creator><creator>Zhou, Dong-hai</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature</general><general>Springer Nature B.V</general><scope>AOWDO</scope><scope>BLEPL</scope><scope>DTL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7SN</scope><scope>7T7</scope><scope>7TV</scope><scope>7U7</scope><scope>7WY</scope><scope>7WZ</scope><scope>7X7</scope><scope>7XB</scope><scope>87Z</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8FL</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BEZIV</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FRNLG</scope><scope>FYUFA</scope><scope>F~G</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K60</scope><scope>K6~</scope><scope>K9.</scope><scope>L.-</scope><scope>M0C</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>P64</scope><scope>PATMY</scope><scope>PQBIZ</scope><scope>PQBZA</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope><scope>Q9U</scope></search><sort><creationdate>20201101</creationdate><title>Antagonistic effects of nano-selenium on broilers hepatic injury induced by Cr(VI) poisoning in AMPK pathway</title><author>Zhang, Tian-guang ; Zhao, Ya-li ; Li, Lei ; Zhou, Dong-hai</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c412t-cd3ca1f39f6f704ddc88947d4d36e0dab60ae7a9da6c648e5474c364f57bb4d13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Adenosine kinase</topic><topic>Adenosine monophosphate</topic><topic>AMP</topic><topic>AMP-Activated Protein Kinases - genetics</topic><topic>AMP-Activated Protein Kinases - metabolism</topic><topic>Animals</topic><topic>Aquatic Pollution</topic><topic>Atmospheric Protection/Air Quality Control/Air Pollution</topic><topic>Carbohydrate metabolism</topic><topic>Carbohydrates</topic><topic>Chickens</topic><topic>Chromium</topic><topic>Chromium - metabolism</topic><topic>Chromium - toxicity</topic><topic>Earth and Environmental Science</topic><topic>Ecotoxicology</topic><topic>Environment</topic><topic>Environmental Chemistry</topic><topic>Environmental Health</topic><topic>Environmental science</topic><topic>Environmental Sciences</topic><topic>Environmental Sciences & Ecology</topic><topic>Feed additives</topic><topic>Food additives</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Heavy metals</topic><topic>Hepatocytes</topic><topic>Hexavalent chromium</topic><topic>Immunohistochemistry</topic><topic>Injuries</topic><topic>Kinases</topic><topic>Life Sciences & Biomedicine</topic><topic>Lipid Metabolism</topic><topic>Lipids</topic><topic>Liver</topic><topic>Liver - metabolism</topic><topic>Liver diseases</topic><topic>Metabolic disorders</topic><topic>Poisoning</topic><topic>Poultry</topic><topic>Poultry feed</topic><topic>Protein kinase</topic><topic>Proteins</topic><topic>Research Article</topic><topic>Science & Technology</topic><topic>Selenium</topic><topic>Selenium - metabolism</topic><topic>Valence</topic><topic>Waste Water Technology</topic><topic>Water Management</topic><topic>Water Pollution Control</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Tian-guang</creatorcontrib><creatorcontrib>Zhao, Ya-li</creatorcontrib><creatorcontrib>Li, Lei</creatorcontrib><creatorcontrib>Zhou, Dong-hai</creatorcontrib><collection>Web of Science - Science Citation Index Expanded - 2020</collection><collection>Web of Science Core Collection</collection><collection>Science Citation Index Expanded</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Ecology 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Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Business Premium Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Business Premium Collection (Alumni)</collection><collection>Health Research Premium Collection</collection><collection>ABI/INFORM Global (Corporate)</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Business Collection (Alumni Edition)</collection><collection>ProQuest Business Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ABI/INFORM Professional Advanced</collection><collection>ABI/INFORM Global</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>ProQuest One Business</collection><collection>ProQuest One Business (Alumni)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Environmental Science Collection</collection><collection>ProQuest Central Basic</collection><jtitle>Environmental science and pollution research international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Tian-guang</au><au>Zhao, Ya-li</au><au>Li, Lei</au><au>Zhou, Dong-hai</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antagonistic effects of nano-selenium on broilers hepatic injury induced by Cr(VI) poisoning in AMPK pathway</atitle><jtitle>Environmental science and pollution research international</jtitle><stitle>Environ Sci Pollut Res</stitle><stitle>ENVIRON SCI POLLUT R</stitle><addtitle>Environ Sci Pollut Res Int</addtitle><date>2020-11-01</date><risdate>2020</risdate><volume>27</volume><issue>33</issue><spage>41585</spage><epage>41595</epage><pages>41585-41595</pages><issn>0944-1344</issn><eissn>1614-7499</eissn><abstract>Cr (chromium, with common valence states of III and VI) is one of the common broiler feed additives. Liver injury and metabolic disorders could be caused by Cr
(VI)
(hexavalent chromium) poisoning in broilers. Oxidative damage and metabolic disorders of organisms caused by heavy metals could be antagonized by nano-Se (nano-selenium). Nano-Se was chosen to study the antagonism of Cr
(VI)
poisoning in broilers. AMPK (Adenosine 5
,
-monophosphate-activated protein kinase) is known as a “cell energy regulator” and plays a key regulatory role in carbohydrate and lipid metabolism. AMPK pathway and
ACACA
/
CPT1A
two genes were selected to study the prevention and treatment of nano-Se on Cr
(VI)
poisoning in broilers and its molecular mechanism. For this purpose, 180 1-day-old AA (Arbor Acres) broilers were selected and randomly divided into 6 groups (
n
= 30) for further testing. After feeding as planned for 35 days, the livers of such broilers were taken for further examination including histopathological examination, differential gene expression analysis, and further validation on both mRNA and protein levels using related techniques like RT-qPCR, western blot, and immunohistochemistry (IHC). The histopathological examination suggested that the liver cells of the Cr
(VI)
poisoning group were more severely injured than the nano-Se addition group. RT-qPCR results showed that the relative expression of
ACACA
gene in the Cr
(VI)
poisoning group was significantly increased (
P
< 0.05), while the
CPT1A
gene’s expression was significantly decreased (
P
< 0.01). Those results were reversed in the nano-Se addition group. Western blot results were consistent with RT-qPCR and both suggested antagonism of nano-Se on Cr
(VI)
. Through morphological and histopathological observation, as well as the measurement of the mRNA and protein expression levels of
ACACA
and
CPT1A
genes in AMPK pathway, it was confirmed that nano-Se has certain preventive and protective effects on Cr
(VI)
poisoning in broiler chickens. Furthermore, the adverse effects of Cr
(VI)
on carbohydrate and lipid metabolism in broilers can be antagonized by nano-Se through AMPK pathway. A new method and experimental basis were provided to the future study of Cr
(VI)
poisoning in broilers.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>32691314</pmid><doi>10.1007/s11356-020-08501-0</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0944-1344 |
| ispartof | Environmental science and pollution research international, 2020-11, Vol.27 (33), p.41585-41595 |
| issn | 0944-1344 1614-7499 |
| language | eng |
| recordid | cdi_pubmed_primary_32691314 |
| source | MEDLINE; Web of Science - Science Citation Index Expanded - 2020<img src="https://exlibris-pub.s3.amazonaws.com/fromwos-v2.jpg" />; SpringerLink Journals - AutoHoldings |
| subjects | Adenosine kinase Adenosine monophosphate AMP AMP-Activated Protein Kinases - genetics AMP-Activated Protein Kinases - metabolism Animals Aquatic Pollution Atmospheric Protection/Air Quality Control/Air Pollution Carbohydrate metabolism Carbohydrates Chickens Chromium Chromium - metabolism Chromium - toxicity Earth and Environmental Science Ecotoxicology Environment Environmental Chemistry Environmental Health Environmental science Environmental Sciences Environmental Sciences & Ecology Feed additives Food additives Gene expression Genes Heavy metals Hepatocytes Hexavalent chromium Immunohistochemistry Injuries Kinases Life Sciences & Biomedicine Lipid Metabolism Lipids Liver Liver - metabolism Liver diseases Metabolic disorders Poisoning Poultry Poultry feed Protein kinase Proteins Research Article Science & Technology Selenium Selenium - metabolism Valence Waste Water Technology Water Management Water Pollution Control |
| title | Antagonistic effects of nano-selenium on broilers hepatic injury induced by Cr(VI) poisoning in AMPK pathway |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-13T02%3A12%3A56IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Antagonistic%20effects%20of%20nano-selenium%20on%20broilers%20hepatic%20injury%20induced%20by%20Cr(VI)%20poisoning%20in%20AMPK%20pathway&rft.jtitle=Environmental%20science%20and%20pollution%20research%20international&rft.au=Zhang,%20Tian-guang&rft.date=2020-11-01&rft.volume=27&rft.issue=33&rft.spage=41585&rft.epage=41595&rft.pages=41585-41595&rft.issn=0944-1344&rft.eissn=1614-7499&rft_id=info:doi/10.1007/s11356-020-08501-0&rft_dat=%3Cproquest_pubme%3E2471534334%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2471534334&rft_id=info:pmid/32691314&rfr_iscdi=true |