Changes in the result of antinuclear antibody immunofluorescence assay on HEp-2 cells reflect disease activity status in systemic lupus erythematosus
Background: The objective of the study was to determine whether the staining pattern and titer of indirect immunofluorescence assay on HEp-2 cells (HEp-2 IFA) are associated with systemic lupus erythematosus (SLE) disease activity. Methods: A total of 269 consecutive patients meeting the ACR and SLI...
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| Veröffentlicht in: | Clinical chemistry and laboratory medicine 2020-08, Vol.58 (8), p.1271-1281 |
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| creator | Prado, Mônica Simon Dellavance, Alessandra Rodrigues, Silvia Helena Marvulle, Valdecir Andrade, Luis Eduardo Coelho |
| description | Background: The objective of the study was to determine whether the staining pattern and titer of indirect immunofluorescence assay on HEp-2 cells (HEp-2 IFA) are associated with systemic lupus erythematosus (SLE) disease activity.
Methods: A total of 269 consecutive patients meeting the ACR and SLICC criteria for SLE were classified into three groups according to the SLE Disease Activity Index 2000 (SLEDAI2K): Remission (SLEDAI2K = 0; n = 47); Intermediate (SLEDAI2K = 1-5; n = 111); Active (SLEDAI2K >= 6; n = 111). All subjects were assessed for HEp-2 IFA titer and staining pattern and nine traditional parameters of SLE disease activity. After a 6 to 12-month interval, 101 of the 269 patients were reassessed.
Results: HEp-2 IFA homogeneous nuclear pattern (AC-1) occurred more frequently in the Active Group compared to the Remission Group (p < 0.001). Fine speckled nuclear pattern (AC-4) tended to occur more frequently in the Remission Group compared to the Active Group (p = 0.054). Subjects with AC-1 pattern had higher SLEDAI (8.8 +/- 7.6) than those with AC-4 (4.8 +/- 5.2) (p < 0.001). HEp-2 IFA titer and anti-nuclear antibody by enzyme-linked immunosorbent assay (ANA-ELISA) values were lower in the Remission Group compared to the other two groups ( p < 0.001). Multivariate analyses identified only ELISA anti-dsDNA as an independent variable associated with disease activity. In follow-up analysis, HEp-2 IFA titer decreased significantly in the 33 subjects with decreased disease activity (p = 0.002). Receiver operator characteristic (ROC) curve analysis for determination of disease activity showed equivalent areas under the curve (AUC) for HEp-2 IFA titer and traditional disease activity parameters.
Conclusions: HEp-2 IFA pattern and titer can reflect SLE disease activity and may be considered in conjunction with other laboratory and clinical parameters in the assessment of SLE disease activity. |
| doi_str_mv | 10.1515/cclm-2019-0638 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmed_primary_32623848</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2420186794</sourcerecordid><originalsourceid>FETCH-LOGICAL-c443t-ee4d3143f48cf537975d2ccd6197067fd299b54633183750bca02d561d24fa623</originalsourceid><addsrcrecordid>eNqNkU2L1TAYhYsozoduXUrAjTB0zFeTduFCyugIA250XdI0ncmQJte8yUh_iP_X9N7rCOLCVQ7hed_knFNVrwi-JA1p3mntlppi0tVYsPZJdUo4kzVnjDzda14LQclJdQZwjzFpGi6fVyeMCspa3p5WP_s75W8NIOtRujMoGsguoTAj5ZP1WTuj4l6PYVqRXZbsw-xyKKA2XhukANSKgkfXV7uaIm2cg7JmdkYnNFkwCgqkk32waUWQVMr712CFZBarkcu7cmPiWt5fVAqQ4UX1bFYOzMvjeV59-3j1tb-ub758-tx_uKk15yzVxvCJFZMzb_XcMNnJZqJaT4J0Egs5T7TrxoaLkkbLZINHrTCdGkEmymdVIjiv3h727mL4ng2kYbGwOVDehAwD5RQLLkqMBX3zF3ofcvTld3uKtEJ2G3V5oHQMACWFYRftouI6EDxshQ1bYcNW2LAVVgZeH9fmcTHTI_67oQJcHIAfZgwzaLuF_ohhjDd_XIqi6Gao_X-6t6UMG3wfsk9l9P1xVLlk4mRuY16L-OPz3xaatiVUEvYLMLfIXg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2420186794</pqid></control><display><type>article</type><title>Changes in the result of antinuclear antibody immunofluorescence assay on HEp-2 cells reflect disease activity status in systemic lupus erythematosus</title><source>Web of Science - Science Citation Index Expanded - 2020<img src="https://exlibris-pub.s3.amazonaws.com/fromwos-v2.jpg" /></source><source>De Gruyter journals</source><creator>Prado, Mônica Simon ; Dellavance, Alessandra ; Rodrigues, Silvia Helena ; Marvulle, Valdecir ; Andrade, Luis Eduardo Coelho</creator><creatorcontrib>Prado, Mônica Simon ; Dellavance, Alessandra ; Rodrigues, Silvia Helena ; Marvulle, Valdecir ; Andrade, Luis Eduardo Coelho</creatorcontrib><description>Background: The objective of the study was to determine whether the staining pattern and titer of indirect immunofluorescence assay on HEp-2 cells (HEp-2 IFA) are associated with systemic lupus erythematosus (SLE) disease activity.
Methods: A total of 269 consecutive patients meeting the ACR and SLICC criteria for SLE were classified into three groups according to the SLE Disease Activity Index 2000 (SLEDAI2K): Remission (SLEDAI2K = 0; n = 47); Intermediate (SLEDAI2K = 1-5; n = 111); Active (SLEDAI2K >= 6; n = 111). All subjects were assessed for HEp-2 IFA titer and staining pattern and nine traditional parameters of SLE disease activity. After a 6 to 12-month interval, 101 of the 269 patients were reassessed.
Results: HEp-2 IFA homogeneous nuclear pattern (AC-1) occurred more frequently in the Active Group compared to the Remission Group (p < 0.001). Fine speckled nuclear pattern (AC-4) tended to occur more frequently in the Remission Group compared to the Active Group (p = 0.054). Subjects with AC-1 pattern had higher SLEDAI (8.8 +/- 7.6) than those with AC-4 (4.8 +/- 5.2) (p < 0.001). HEp-2 IFA titer and anti-nuclear antibody by enzyme-linked immunosorbent assay (ANA-ELISA) values were lower in the Remission Group compared to the other two groups ( p < 0.001). Multivariate analyses identified only ELISA anti-dsDNA as an independent variable associated with disease activity. In follow-up analysis, HEp-2 IFA titer decreased significantly in the 33 subjects with decreased disease activity (p = 0.002). Receiver operator characteristic (ROC) curve analysis for determination of disease activity showed equivalent areas under the curve (AUC) for HEp-2 IFA titer and traditional disease activity parameters.
Conclusions: HEp-2 IFA pattern and titer can reflect SLE disease activity and may be considered in conjunction with other laboratory and clinical parameters in the assessment of SLE disease activity.</description><identifier>ISSN: 1434-6621</identifier><identifier>EISSN: 1437-4331</identifier><identifier>DOI: 10.1515/cclm-2019-0638</identifier><identifier>PMID: 32623848</identifier><language>eng</language><publisher>BERLIN: De Gruyter</publisher><subject>Anti-DNA antibodies ; Antibodies ; Antinuclear antibodies ; antinuclear antibody ; Assaying ; autoantibodies ; Autoimmune diseases ; Chronic conditions ; disease activity ; Enzyme-linked immunosorbent assay ; Health risk assessment ; HEp-2 cells ; Immunofluorescence ; Independent variables ; Life Sciences & Biomedicine ; Lupus ; Medical Laboratory Technology ; Parameters ; Remission ; Science & Technology ; SLEDAI2K ; Staining ; Systemic lupus erythematosus</subject><ispartof>Clinical chemistry and laboratory medicine, 2020-08, Vol.58 (8), p.1271-1281</ispartof><rights>2020 Walter de Gruyter GmbH, Berlin/Boston</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>true</woscitedreferencessubscribed><woscitedreferencescount>14</woscitedreferencescount><woscitedreferencesoriginalsourcerecordid>wos000546347600022</woscitedreferencesoriginalsourcerecordid><citedby>FETCH-LOGICAL-c443t-ee4d3143f48cf537975d2ccd6197067fd299b54633183750bca02d561d24fa623</citedby><cites>FETCH-LOGICAL-c443t-ee4d3143f48cf537975d2ccd6197067fd299b54633183750bca02d561d24fa623</cites><orcidid>0000-0001-8742-9931</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.degruyter.com/document/doi/10.1515/cclm-2019-0638/pdf$$EPDF$$P50$$Gwalterdegruyter$$H</linktopdf><linktohtml>$$Uhttps://www.degruyter.com/document/doi/10.1515/cclm-2019-0638/html$$EHTML$$P50$$Gwalterdegruyter$$H</linktohtml><link.rule.ids>315,781,785,27929,27930,28253,66759,68543</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32623848$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Prado, Mônica Simon</creatorcontrib><creatorcontrib>Dellavance, Alessandra</creatorcontrib><creatorcontrib>Rodrigues, Silvia Helena</creatorcontrib><creatorcontrib>Marvulle, Valdecir</creatorcontrib><creatorcontrib>Andrade, Luis Eduardo Coelho</creatorcontrib><title>Changes in the result of antinuclear antibody immunofluorescence assay on HEp-2 cells reflect disease activity status in systemic lupus erythematosus</title><title>Clinical chemistry and laboratory medicine</title><addtitle>CLIN CHEM LAB MED</addtitle><addtitle>Clin Chem Lab Med</addtitle><description>Background: The objective of the study was to determine whether the staining pattern and titer of indirect immunofluorescence assay on HEp-2 cells (HEp-2 IFA) are associated with systemic lupus erythematosus (SLE) disease activity.
Methods: A total of 269 consecutive patients meeting the ACR and SLICC criteria for SLE were classified into three groups according to the SLE Disease Activity Index 2000 (SLEDAI2K): Remission (SLEDAI2K = 0; n = 47); Intermediate (SLEDAI2K = 1-5; n = 111); Active (SLEDAI2K >= 6; n = 111). All subjects were assessed for HEp-2 IFA titer and staining pattern and nine traditional parameters of SLE disease activity. After a 6 to 12-month interval, 101 of the 269 patients were reassessed.
Results: HEp-2 IFA homogeneous nuclear pattern (AC-1) occurred more frequently in the Active Group compared to the Remission Group (p < 0.001). Fine speckled nuclear pattern (AC-4) tended to occur more frequently in the Remission Group compared to the Active Group (p = 0.054). Subjects with AC-1 pattern had higher SLEDAI (8.8 +/- 7.6) than those with AC-4 (4.8 +/- 5.2) (p < 0.001). HEp-2 IFA titer and anti-nuclear antibody by enzyme-linked immunosorbent assay (ANA-ELISA) values were lower in the Remission Group compared to the other two groups ( p < 0.001). Multivariate analyses identified only ELISA anti-dsDNA as an independent variable associated with disease activity. In follow-up analysis, HEp-2 IFA titer decreased significantly in the 33 subjects with decreased disease activity (p = 0.002). Receiver operator characteristic (ROC) curve analysis for determination of disease activity showed equivalent areas under the curve (AUC) for HEp-2 IFA titer and traditional disease activity parameters.
Conclusions: HEp-2 IFA pattern and titer can reflect SLE disease activity and may be considered in conjunction with other laboratory and clinical parameters in the assessment of SLE disease activity.</description><subject>Anti-DNA antibodies</subject><subject>Antibodies</subject><subject>Antinuclear antibodies</subject><subject>antinuclear antibody</subject><subject>Assaying</subject><subject>autoantibodies</subject><subject>Autoimmune diseases</subject><subject>Chronic conditions</subject><subject>disease activity</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Health risk assessment</subject><subject>HEp-2 cells</subject><subject>Immunofluorescence</subject><subject>Independent variables</subject><subject>Life Sciences & Biomedicine</subject><subject>Lupus</subject><subject>Medical Laboratory Technology</subject><subject>Parameters</subject><subject>Remission</subject><subject>Science & Technology</subject><subject>SLEDAI2K</subject><subject>Staining</subject><subject>Systemic lupus erythematosus</subject><issn>1434-6621</issn><issn>1437-4331</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>AOWDO</sourceid><recordid>eNqNkU2L1TAYhYsozoduXUrAjTB0zFeTduFCyugIA250XdI0ncmQJte8yUh_iP_X9N7rCOLCVQ7hed_knFNVrwi-JA1p3mntlppi0tVYsPZJdUo4kzVnjDzda14LQclJdQZwjzFpGi6fVyeMCspa3p5WP_s75W8NIOtRujMoGsguoTAj5ZP1WTuj4l6PYVqRXZbsw-xyKKA2XhukANSKgkfXV7uaIm2cg7JmdkYnNFkwCgqkk32waUWQVMr712CFZBarkcu7cmPiWt5fVAqQ4UX1bFYOzMvjeV59-3j1tb-ub758-tx_uKk15yzVxvCJFZMzb_XcMNnJZqJaT4J0Egs5T7TrxoaLkkbLZINHrTCdGkEmymdVIjiv3h727mL4ng2kYbGwOVDehAwD5RQLLkqMBX3zF3ofcvTld3uKtEJ2G3V5oHQMACWFYRftouI6EDxshQ1bYcNW2LAVVgZeH9fmcTHTI_67oQJcHIAfZgwzaLuF_ohhjDd_XIqi6Gao_X-6t6UMG3wfsk9l9P1xVLlk4mRuY16L-OPz3xaatiVUEvYLMLfIXg</recordid><startdate>20200801</startdate><enddate>20200801</enddate><creator>Prado, Mônica Simon</creator><creator>Dellavance, Alessandra</creator><creator>Rodrigues, Silvia Helena</creator><creator>Marvulle, Valdecir</creator><creator>Andrade, Luis Eduardo Coelho</creator><general>De Gruyter</general><general>Walter De Gruyter</general><general>Walter De Gruyter & Company</general><scope>AOWDO</scope><scope>BLEPL</scope><scope>DTL</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T7</scope><scope>7TK</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-8742-9931</orcidid></search><sort><creationdate>20200801</creationdate><title>Changes in the result of antinuclear antibody immunofluorescence assay on HEp-2 cells reflect disease activity status in systemic lupus erythematosus</title><author>Prado, Mônica Simon ; Dellavance, Alessandra ; Rodrigues, Silvia Helena ; Marvulle, Valdecir ; Andrade, Luis Eduardo Coelho</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c443t-ee4d3143f48cf537975d2ccd6197067fd299b54633183750bca02d561d24fa623</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Anti-DNA antibodies</topic><topic>Antibodies</topic><topic>Antinuclear antibodies</topic><topic>antinuclear antibody</topic><topic>Assaying</topic><topic>autoantibodies</topic><topic>Autoimmune diseases</topic><topic>Chronic conditions</topic><topic>disease activity</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Health risk assessment</topic><topic>HEp-2 cells</topic><topic>Immunofluorescence</topic><topic>Independent variables</topic><topic>Life Sciences & Biomedicine</topic><topic>Lupus</topic><topic>Medical Laboratory Technology</topic><topic>Parameters</topic><topic>Remission</topic><topic>Science & Technology</topic><topic>SLEDAI2K</topic><topic>Staining</topic><topic>Systemic lupus erythematosus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Prado, Mônica Simon</creatorcontrib><creatorcontrib>Dellavance, Alessandra</creatorcontrib><creatorcontrib>Rodrigues, Silvia Helena</creatorcontrib><creatorcontrib>Marvulle, Valdecir</creatorcontrib><creatorcontrib>Andrade, Luis Eduardo Coelho</creatorcontrib><collection>Web of Science - Science Citation Index Expanded - 2020</collection><collection>Web of Science Core Collection</collection><collection>Science Citation Index Expanded</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical chemistry and laboratory medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Prado, Mônica Simon</au><au>Dellavance, Alessandra</au><au>Rodrigues, Silvia Helena</au><au>Marvulle, Valdecir</au><au>Andrade, Luis Eduardo Coelho</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Changes in the result of antinuclear antibody immunofluorescence assay on HEp-2 cells reflect disease activity status in systemic lupus erythematosus</atitle><jtitle>Clinical chemistry and laboratory medicine</jtitle><stitle>CLIN CHEM LAB MED</stitle><addtitle>Clin Chem Lab Med</addtitle><date>2020-08-01</date><risdate>2020</risdate><volume>58</volume><issue>8</issue><spage>1271</spage><epage>1281</epage><pages>1271-1281</pages><issn>1434-6621</issn><eissn>1437-4331</eissn><abstract>Background: The objective of the study was to determine whether the staining pattern and titer of indirect immunofluorescence assay on HEp-2 cells (HEp-2 IFA) are associated with systemic lupus erythematosus (SLE) disease activity.
Methods: A total of 269 consecutive patients meeting the ACR and SLICC criteria for SLE were classified into three groups according to the SLE Disease Activity Index 2000 (SLEDAI2K): Remission (SLEDAI2K = 0; n = 47); Intermediate (SLEDAI2K = 1-5; n = 111); Active (SLEDAI2K >= 6; n = 111). All subjects were assessed for HEp-2 IFA titer and staining pattern and nine traditional parameters of SLE disease activity. After a 6 to 12-month interval, 101 of the 269 patients were reassessed.
Results: HEp-2 IFA homogeneous nuclear pattern (AC-1) occurred more frequently in the Active Group compared to the Remission Group (p < 0.001). Fine speckled nuclear pattern (AC-4) tended to occur more frequently in the Remission Group compared to the Active Group (p = 0.054). Subjects with AC-1 pattern had higher SLEDAI (8.8 +/- 7.6) than those with AC-4 (4.8 +/- 5.2) (p < 0.001). HEp-2 IFA titer and anti-nuclear antibody by enzyme-linked immunosorbent assay (ANA-ELISA) values were lower in the Remission Group compared to the other two groups ( p < 0.001). Multivariate analyses identified only ELISA anti-dsDNA as an independent variable associated with disease activity. In follow-up analysis, HEp-2 IFA titer decreased significantly in the 33 subjects with decreased disease activity (p = 0.002). Receiver operator characteristic (ROC) curve analysis for determination of disease activity showed equivalent areas under the curve (AUC) for HEp-2 IFA titer and traditional disease activity parameters.
Conclusions: HEp-2 IFA pattern and titer can reflect SLE disease activity and may be considered in conjunction with other laboratory and clinical parameters in the assessment of SLE disease activity.</abstract><cop>BERLIN</cop><pub>De Gruyter</pub><pmid>32623848</pmid><doi>10.1515/cclm-2019-0638</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0001-8742-9931</orcidid></addata></record> |
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| source | Web of Science - Science Citation Index Expanded - 2020<img src="https://exlibris-pub.s3.amazonaws.com/fromwos-v2.jpg" />; De Gruyter journals |
| subjects | Anti-DNA antibodies Antibodies Antinuclear antibodies antinuclear antibody Assaying autoantibodies Autoimmune diseases Chronic conditions disease activity Enzyme-linked immunosorbent assay Health risk assessment HEp-2 cells Immunofluorescence Independent variables Life Sciences & Biomedicine Lupus Medical Laboratory Technology Parameters Remission Science & Technology SLEDAI2K Staining Systemic lupus erythematosus |
| title | Changes in the result of antinuclear antibody immunofluorescence assay on HEp-2 cells reflect disease activity status in systemic lupus erythematosus |
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