Morphological and functional abnormalities of hippocampus in APC 1638T/1638T mice
In the present study, we examined morphology and function of hippocampus in the APC mouse. Expression levels of the APC mRNA and protein were both identical in the hippocampus of the APC and APC mice. The dentate gyrus of the APC hippocampus was thicker, and has more densely-populated granule cells...
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| Veröffentlicht in: | Medical molecular morphology 2021-03, Vol.54 (1), p.31 |
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| creator | Li, Chenguang Onouchi, Takanori Hirayama, Masaya Sakai, Kazuyoshi Matsuda, Shuji Yamada, Nami O Senda, Takao |
| description | In the present study, we examined morphology and function of hippocampus in the APC
mouse. Expression levels of the APC mRNA and protein were both identical in the hippocampus of the APC
and APC
mice. The dentate gyrus of the APC
hippocampus was thicker, and has more densely-populated granule cells in the APC
mouse hippocampus. Immunoelectron microscopy revealed co-localization of APC with alpha-amino-3- hydroxy-5-methyl- isoxazole-4-propionate receptor (AMPA-R) and with PSD-95 at post-synapse in the APC
hippocampus, while APC1638T was co-localized with neither AMPA-R nor PSD-95 in the APC
hippocampus. By immunoprecipitation assay, full-length APC expressed in the APC
mouse was co-immunoprecipitated with AMPA-R and PSD-95. In contrast, APC1638T expressed in the APC
mouse was not co-immunoprecipitated with AMPA-R and PSD-95. In the hippocampal CA1 region of the APC
mouse, c-Fos expression after electric foot shock was decreased compared with the APC
mouse. The present study showed some abnormalities on morphology of the hippocampus caused by a truncated APC (APC1638T). Also, our findings suggest that failure in APC binding to AMPA-R and PSD-95 may bring about less activities of hippocampal neurons in the APC
mouse. |
| doi_str_mv | 10.1007/s00795-020-00257-3 |
| format | Article |
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mouse. Expression levels of the APC mRNA and protein were both identical in the hippocampus of the APC
and APC
mice. The dentate gyrus of the APC
hippocampus was thicker, and has more densely-populated granule cells in the APC
mouse hippocampus. Immunoelectron microscopy revealed co-localization of APC with alpha-amino-3- hydroxy-5-methyl- isoxazole-4-propionate receptor (AMPA-R) and with PSD-95 at post-synapse in the APC
hippocampus, while APC1638T was co-localized with neither AMPA-R nor PSD-95 in the APC
hippocampus. By immunoprecipitation assay, full-length APC expressed in the APC
mouse was co-immunoprecipitated with AMPA-R and PSD-95. In contrast, APC1638T expressed in the APC
mouse was not co-immunoprecipitated with AMPA-R and PSD-95. In the hippocampal CA1 region of the APC
mouse, c-Fos expression after electric foot shock was decreased compared with the APC
mouse. The present study showed some abnormalities on morphology of the hippocampus caused by a truncated APC (APC1638T). Also, our findings suggest that failure in APC binding to AMPA-R and PSD-95 may bring about less activities of hippocampal neurons in the APC
mouse.</description><identifier>EISSN: 1860-1499</identifier><identifier>DOI: 10.1007/s00795-020-00257-3</identifier><identifier>PMID: 32572622</identifier><language>eng</language><publisher>Japan</publisher><ispartof>Medical molecular morphology, 2021-03, Vol.54 (1), p.31</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27911,27912</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32572622$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Chenguang</creatorcontrib><creatorcontrib>Onouchi, Takanori</creatorcontrib><creatorcontrib>Hirayama, Masaya</creatorcontrib><creatorcontrib>Sakai, Kazuyoshi</creatorcontrib><creatorcontrib>Matsuda, Shuji</creatorcontrib><creatorcontrib>Yamada, Nami O</creatorcontrib><creatorcontrib>Senda, Takao</creatorcontrib><title>Morphological and functional abnormalities of hippocampus in APC 1638T/1638T mice</title><title>Medical molecular morphology</title><addtitle>Med Mol Morphol</addtitle><description>In the present study, we examined morphology and function of hippocampus in the APC
mouse. Expression levels of the APC mRNA and protein were both identical in the hippocampus of the APC
and APC
mice. The dentate gyrus of the APC
hippocampus was thicker, and has more densely-populated granule cells in the APC
mouse hippocampus. Immunoelectron microscopy revealed co-localization of APC with alpha-amino-3- hydroxy-5-methyl- isoxazole-4-propionate receptor (AMPA-R) and with PSD-95 at post-synapse in the APC
hippocampus, while APC1638T was co-localized with neither AMPA-R nor PSD-95 in the APC
hippocampus. By immunoprecipitation assay, full-length APC expressed in the APC
mouse was co-immunoprecipitated with AMPA-R and PSD-95. In contrast, APC1638T expressed in the APC
mouse was not co-immunoprecipitated with AMPA-R and PSD-95. In the hippocampal CA1 region of the APC
mouse, c-Fos expression after electric foot shock was decreased compared with the APC
mouse. The present study showed some abnormalities on morphology of the hippocampus caused by a truncated APC (APC1638T). Also, our findings suggest that failure in APC binding to AMPA-R and PSD-95 may bring about less activities of hippocampal neurons in the APC
mouse.</description><issn>1860-1499</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNqFjrsKwjAYRoMg1tsLOEheIPZPYls7iigugoK7pG1qI7nR2MG394LOLufwwRk-hGYUFhQgi8MLeUKAAQFgSUZ4Dw3pKgVCl3keoVEINwCepSwZoIi_CpYyNkSng2t947S7qlJoLGyF686Wd-XsexbWtUZodVcyYFfjRnnvSmF8F7CyeH3cYJry1Tn-EBtVygnq10IHOf16jOa77XmzJ74rjKwuvlVGtI_L7wP_GzwBEt5AaQ</recordid><startdate>202103</startdate><enddate>202103</enddate><creator>Li, Chenguang</creator><creator>Onouchi, Takanori</creator><creator>Hirayama, Masaya</creator><creator>Sakai, Kazuyoshi</creator><creator>Matsuda, Shuji</creator><creator>Yamada, Nami O</creator><creator>Senda, Takao</creator><scope>NPM</scope></search><sort><creationdate>202103</creationdate><title>Morphological and functional abnormalities of hippocampus in APC 1638T/1638T mice</title><author>Li, Chenguang ; Onouchi, Takanori ; Hirayama, Masaya ; Sakai, Kazuyoshi ; Matsuda, Shuji ; Yamada, Nami O ; Senda, Takao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-pubmed_primary_325726223</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Chenguang</creatorcontrib><creatorcontrib>Onouchi, Takanori</creatorcontrib><creatorcontrib>Hirayama, Masaya</creatorcontrib><creatorcontrib>Sakai, Kazuyoshi</creatorcontrib><creatorcontrib>Matsuda, Shuji</creatorcontrib><creatorcontrib>Yamada, Nami O</creatorcontrib><creatorcontrib>Senda, Takao</creatorcontrib><collection>PubMed</collection><jtitle>Medical molecular morphology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Chenguang</au><au>Onouchi, Takanori</au><au>Hirayama, Masaya</au><au>Sakai, Kazuyoshi</au><au>Matsuda, Shuji</au><au>Yamada, Nami O</au><au>Senda, Takao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Morphological and functional abnormalities of hippocampus in APC 1638T/1638T mice</atitle><jtitle>Medical molecular morphology</jtitle><addtitle>Med Mol Morphol</addtitle><date>2021-03</date><risdate>2021</risdate><volume>54</volume><issue>1</issue><spage>31</spage><pages>31-</pages><eissn>1860-1499</eissn><abstract>In the present study, we examined morphology and function of hippocampus in the APC
mouse. Expression levels of the APC mRNA and protein were both identical in the hippocampus of the APC
and APC
mice. The dentate gyrus of the APC
hippocampus was thicker, and has more densely-populated granule cells in the APC
mouse hippocampus. Immunoelectron microscopy revealed co-localization of APC with alpha-amino-3- hydroxy-5-methyl- isoxazole-4-propionate receptor (AMPA-R) and with PSD-95 at post-synapse in the APC
hippocampus, while APC1638T was co-localized with neither AMPA-R nor PSD-95 in the APC
hippocampus. By immunoprecipitation assay, full-length APC expressed in the APC
mouse was co-immunoprecipitated with AMPA-R and PSD-95. In contrast, APC1638T expressed in the APC
mouse was not co-immunoprecipitated with AMPA-R and PSD-95. In the hippocampal CA1 region of the APC
mouse, c-Fos expression after electric foot shock was decreased compared with the APC
mouse. The present study showed some abnormalities on morphology of the hippocampus caused by a truncated APC (APC1638T). Also, our findings suggest that failure in APC binding to AMPA-R and PSD-95 may bring about less activities of hippocampal neurons in the APC
mouse.</abstract><cop>Japan</cop><pmid>32572622</pmid><doi>10.1007/s00795-020-00257-3</doi></addata></record> |
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| ispartof | Medical molecular morphology, 2021-03, Vol.54 (1), p.31 |
| issn | 1860-1499 |
| language | eng |
| recordid | cdi_pubmed_primary_32572622 |
| source | Springer Nature - Complete Springer Journals |
| title | Morphological and functional abnormalities of hippocampus in APC 1638T/1638T mice |
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