Single-chain variable fragment antibody-based immunochromatographic strip for rapid detection of fumonisin B 1 in maize samples
A rapid and sensitive immunochromatographic strip (ICS) based on a single-chain variable fragment (scFv) was developed for detecting fumonisin B (FB ). The ICS was based on a competitive reaction for colloidal gold-labeled scFv between FB and FB -BSA, which was used along with sheep anti-mouse IgG a...
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| Veröffentlicht in: | Food chemistry 2020-07, Vol.319, p.126546 |
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| container_title | Food chemistry |
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| creator | Ren, Wenjie Xu, Yang Huang, Zhibing Li, Yanping Tu, Zhui Zou, Long He, Qinghua Fu, Jinheng Liu, Shiwen Hammock, Bruce D |
| description | A rapid and sensitive immunochromatographic strip (ICS) based on a single-chain variable fragment (scFv) was developed for detecting fumonisin B
(FB
). The ICS was based on a competitive reaction for colloidal gold-labeled scFv between FB
and FB
-BSA, which was used along with sheep anti-mouse IgG as capture reagents immobilized at test and control lines, respectively, on a nitrocellulose membrane of the strip. The limit of detection of the ICS was 2.5 ng/mL (25 μg/kg) FB
in buffer, and the sensitivity was eight times higher than that of monoclonal antibodies for the preparation of the scFv. The cross-reactivity of the scFv with common mycotoxins was determined by ICS, the results showed that the scFv were not against other mycotoxins. Eight naturally contaminated maize samples were analyzed with the scFv-based ICS and by LC-MS/MS. The results of analysis obtained with the strip assay showed good agreement with those obtained by LC-MS/MS. |
| doi_str_mv | 10.1016/j.foodchem.2020.126546 |
| format | Article |
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(FB
). The ICS was based on a competitive reaction for colloidal gold-labeled scFv between FB
and FB
-BSA, which was used along with sheep anti-mouse IgG as capture reagents immobilized at test and control lines, respectively, on a nitrocellulose membrane of the strip. The limit of detection of the ICS was 2.5 ng/mL (25 μg/kg) FB
in buffer, and the sensitivity was eight times higher than that of monoclonal antibodies for the preparation of the scFv. The cross-reactivity of the scFv with common mycotoxins was determined by ICS, the results showed that the scFv were not against other mycotoxins. Eight naturally contaminated maize samples were analyzed with the scFv-based ICS and by LC-MS/MS. The results of analysis obtained with the strip assay showed good agreement with those obtained by LC-MS/MS.</description><identifier>EISSN: 1873-7072</identifier><identifier>DOI: 10.1016/j.foodchem.2020.126546</identifier><identifier>PMID: 32179370</identifier><language>eng</language><publisher>England</publisher><ispartof>Food chemistry, 2020-07, Vol.319, p.126546</ispartof><rights>Copyright © 2020 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32179370$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ren, Wenjie</creatorcontrib><creatorcontrib>Xu, Yang</creatorcontrib><creatorcontrib>Huang, Zhibing</creatorcontrib><creatorcontrib>Li, Yanping</creatorcontrib><creatorcontrib>Tu, Zhui</creatorcontrib><creatorcontrib>Zou, Long</creatorcontrib><creatorcontrib>He, Qinghua</creatorcontrib><creatorcontrib>Fu, Jinheng</creatorcontrib><creatorcontrib>Liu, Shiwen</creatorcontrib><creatorcontrib>Hammock, Bruce D</creatorcontrib><title>Single-chain variable fragment antibody-based immunochromatographic strip for rapid detection of fumonisin B 1 in maize samples</title><title>Food chemistry</title><addtitle>Food Chem</addtitle><description>A rapid and sensitive immunochromatographic strip (ICS) based on a single-chain variable fragment (scFv) was developed for detecting fumonisin B
(FB
). The ICS was based on a competitive reaction for colloidal gold-labeled scFv between FB
and FB
-BSA, which was used along with sheep anti-mouse IgG as capture reagents immobilized at test and control lines, respectively, on a nitrocellulose membrane of the strip. The limit of detection of the ICS was 2.5 ng/mL (25 μg/kg) FB
in buffer, and the sensitivity was eight times higher than that of monoclonal antibodies for the preparation of the scFv. The cross-reactivity of the scFv with common mycotoxins was determined by ICS, the results showed that the scFv were not against other mycotoxins. Eight naturally contaminated maize samples were analyzed with the scFv-based ICS and by LC-MS/MS. The results of analysis obtained with the strip assay showed good agreement with those obtained by LC-MS/MS.</description><issn>1873-7072</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNo1kMtKAzEYRoMgtlZfoeQFpubSmSRLLWqFggt1Xf7JpZMySYZkKtSNr-6AujpwPjiLD6ElJStKaHN3XLmUjO5sWDHCJsmaet1coDmVgleCCDZD16UcCZlWKq_QjDMqFBdkjr7ffDz0ttId-Ig_IXtoe4tdhkOwccQQR98mc65aKNZgH8IpJt3lFGBMhwxD5zUuY_YDdinjSXiDjR2tHn2KODnsTiFFX6b6A6Z4QgD_ZXGBMPS23KBLB32xt39coI-nx_fNttq9Pr9s7nfVQIkcK7eWyhrjmGkbrYVTWnHiqKuZIlIqVRuuNZPc1TUHodbS1gYMVZJQgMYavkDL3-5waoM1-yH7APm8_3-C_wDgSmRo</recordid><startdate>20200730</startdate><enddate>20200730</enddate><creator>Ren, Wenjie</creator><creator>Xu, Yang</creator><creator>Huang, Zhibing</creator><creator>Li, Yanping</creator><creator>Tu, Zhui</creator><creator>Zou, Long</creator><creator>He, Qinghua</creator><creator>Fu, Jinheng</creator><creator>Liu, Shiwen</creator><creator>Hammock, Bruce D</creator><scope>NPM</scope></search><sort><creationdate>20200730</creationdate><title>Single-chain variable fragment antibody-based immunochromatographic strip for rapid detection of fumonisin B 1 in maize samples</title><author>Ren, Wenjie ; Xu, Yang ; Huang, Zhibing ; Li, Yanping ; Tu, Zhui ; Zou, Long ; He, Qinghua ; Fu, Jinheng ; Liu, Shiwen ; Hammock, Bruce D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p108t-f489eddf2db6cc7f9c930f1f529088995d3cc283f553a7948e5dad19801aa6ed3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ren, Wenjie</creatorcontrib><creatorcontrib>Xu, Yang</creatorcontrib><creatorcontrib>Huang, Zhibing</creatorcontrib><creatorcontrib>Li, Yanping</creatorcontrib><creatorcontrib>Tu, Zhui</creatorcontrib><creatorcontrib>Zou, Long</creatorcontrib><creatorcontrib>He, Qinghua</creatorcontrib><creatorcontrib>Fu, Jinheng</creatorcontrib><creatorcontrib>Liu, Shiwen</creatorcontrib><creatorcontrib>Hammock, Bruce D</creatorcontrib><collection>PubMed</collection><jtitle>Food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ren, Wenjie</au><au>Xu, Yang</au><au>Huang, Zhibing</au><au>Li, Yanping</au><au>Tu, Zhui</au><au>Zou, Long</au><au>He, Qinghua</au><au>Fu, Jinheng</au><au>Liu, Shiwen</au><au>Hammock, Bruce D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Single-chain variable fragment antibody-based immunochromatographic strip for rapid detection of fumonisin B 1 in maize samples</atitle><jtitle>Food chemistry</jtitle><addtitle>Food Chem</addtitle><date>2020-07-30</date><risdate>2020</risdate><volume>319</volume><spage>126546</spage><pages>126546-</pages><eissn>1873-7072</eissn><abstract>A rapid and sensitive immunochromatographic strip (ICS) based on a single-chain variable fragment (scFv) was developed for detecting fumonisin B
(FB
). The ICS was based on a competitive reaction for colloidal gold-labeled scFv between FB
and FB
-BSA, which was used along with sheep anti-mouse IgG as capture reagents immobilized at test and control lines, respectively, on a nitrocellulose membrane of the strip. The limit of detection of the ICS was 2.5 ng/mL (25 μg/kg) FB
in buffer, and the sensitivity was eight times higher than that of monoclonal antibodies for the preparation of the scFv. The cross-reactivity of the scFv with common mycotoxins was determined by ICS, the results showed that the scFv were not against other mycotoxins. Eight naturally contaminated maize samples were analyzed with the scFv-based ICS and by LC-MS/MS. The results of analysis obtained with the strip assay showed good agreement with those obtained by LC-MS/MS.</abstract><cop>England</cop><pmid>32179370</pmid><doi>10.1016/j.foodchem.2020.126546</doi></addata></record> |
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| ispartof | Food chemistry, 2020-07, Vol.319, p.126546 |
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| source | Elsevier ScienceDirect Journals |
| title | Single-chain variable fragment antibody-based immunochromatographic strip for rapid detection of fumonisin B 1 in maize samples |
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