Microscale serum extraction method for the simultaneous analysis of corticosterone and lipids
Corticosterone is an important steroid for the regulation of metabolism and stress response. Existing methods for the measurement of corticosterone include radioimmunoassay (RIA), enzyme-linked immunosorbent assay (ELISA), and liquid chromatography-mass spectrometry (LC-MS). While each of these appr...
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| Veröffentlicht in: | Analytical methods 2019-12, Vol.11 (45), p.5746-5749 |
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| creator | Rister, Alana L Bidne, Katie L Wood, Jennifer R Dodds, Eric D |
| description | Corticosterone is an important steroid for the regulation of metabolism and stress response. Existing methods for the measurement of corticosterone include radioimmunoassay (RIA), enzyme-linked immunosorbent assay (ELISA), and liquid chromatography-mass spectrometry (LC-MS). While each of these approaches have their advantages, RIAs use radioactive isotopes that necessitate specially regulated usage and disposal. Furthermore, both ELISAs and RIAs require expensive kits and can only measure a single analyte. In this study, we establish a new sample preparation technique based on a modified Folch extraction that allows for the simultaneous isolation of corticosterone and lipids from serum. The extract is then analyzed by LC-MS. Using only 5 μL of serum, quantification of corticosterone was achieved with coefficients of variation at 3% or less and a detection limit of 0.12 μM. Overall, the results of this study should be beneficial to the measurement of circulating corticosterone and lipids for a variety of studies using small volumes of samples.
This report details a serum extraction method for the simultaneous analysis of corticosterone and lipids from 5 μL of serum. |
| doi_str_mv | 10.1039/c9ay01757g |
| format | Article |
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This report details a serum extraction method for the simultaneous analysis of corticosterone and lipids from 5 μL of serum.</description><subject>blood serum</subject><subject>Coefficient of variation</subject><subject>Corticosterone</subject><subject>detection limit</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Isotopes</subject><subject>Lipid metabolism</subject><subject>Lipids</subject><subject>Liquid chromatography</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Measurement methods</subject><subject>metabolism</subject><subject>Radioimmunoassay</subject><subject>radioimmunoassays</subject><subject>Radioisotopes</subject><subject>radionuclides</subject><subject>Sample preparation</subject><subject>Steroids</subject><subject>stress response</subject><issn>1759-9660</issn><issn>1759-9679</issn><issn>1759-9679</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNqNks1LHDEYh0Npqbr24t0S8SKF1WQyk4-LIIvaguJFDx4kZPPhRmYma5Ip3f_erLtdWw-lpzfwPPzIm18A2MPoGCMiTrRQC4RZwx4_gO0yxVhQJj5uzhRtgZ2UnhCiglD8GWyRCte8odU2eLj2OoakVWthsnHooP2Vo9LZhx52Ns-CgS5EmGeF-25os-ptGBJUvWoXyScYHNQhZq9DyjaG3hZkYOvn3qRd8MmpNtkv6zkCdxfnt5Pv46ubyx-Ts6uxrjnJYzfVppkSR7jClmPBzZQrQxh1pKqZEbw2FjktXC0QrQimRAvEnVNC14opTUbgdJU7H6adNdr2ZYdWzqPvVFzIoLz8m_R-Jh_DT8lQIyihJeBoHRDD82BTlp1P2rbtaltZEc4Zqzmq_kNlTVM8wot6-E59CkMsD7e0MK0xE6Qp1reVtSwiRes298ZILguWE3F2_1rwZZG__rnpRv3daBH2V0JMekPffkjhB__icm4ceQF75LiN</recordid><startdate>20191207</startdate><enddate>20191207</enddate><creator>Rister, Alana L</creator><creator>Bidne, Katie L</creator><creator>Wood, Jennifer R</creator><creator>Dodds, Eric D</creator><general>Royal Society of Chemistry</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SE</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>FR3</scope><scope>H8G</scope><scope>JG9</scope><scope>L7M</scope><scope>P64</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-6920-6339</orcidid><orcidid>https://orcid.org/0000-0002-7220-0728</orcidid><orcidid>https://orcid.org/0000-0001-9337-5837</orcidid><orcidid>https://orcid.org/0000-0002-7721-394X</orcidid></search><sort><creationdate>20191207</creationdate><title>Microscale serum extraction method for the simultaneous analysis of corticosterone and lipids</title><author>Rister, Alana L ; 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Existing methods for the measurement of corticosterone include radioimmunoassay (RIA), enzyme-linked immunosorbent assay (ELISA), and liquid chromatography-mass spectrometry (LC-MS). While each of these approaches have their advantages, RIAs use radioactive isotopes that necessitate specially regulated usage and disposal. Furthermore, both ELISAs and RIAs require expensive kits and can only measure a single analyte. In this study, we establish a new sample preparation technique based on a modified Folch extraction that allows for the simultaneous isolation of corticosterone and lipids from serum. The extract is then analyzed by LC-MS. Using only 5 μL of serum, quantification of corticosterone was achieved with coefficients of variation at 3% or less and a detection limit of 0.12 μM. Overall, the results of this study should be beneficial to the measurement of circulating corticosterone and lipids for a variety of studies using small volumes of samples.
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| ispartof | Analytical methods, 2019-12, Vol.11 (45), p.5746-5749 |
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| source | Royal Society Of Chemistry Journals 2008- |
| subjects | blood serum Coefficient of variation Corticosterone detection limit Enzyme-linked immunosorbent assay Isotopes Lipid metabolism Lipids Liquid chromatography Mass spectrometry Mass spectroscopy Measurement methods metabolism Radioimmunoassay radioimmunoassays Radioisotopes radionuclides Sample preparation Steroids stress response |
| title | Microscale serum extraction method for the simultaneous analysis of corticosterone and lipids |
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