Iron Overload Mimicking Conditions Skews Bone Marrow Dendritic Cells Differentiation into MHCII low CD11c + CD11b + F4/80 + Cells
Iron overload is an undesired effect of frequent blood transfusions or genetic diseases. Myelodysplastic syndrome (MDS) patients become transfusion dependent, but due to the combination of ineffective haematopoiesis and repeated blood transfusions they are often subject to iron overload. In this stu...
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| Veröffentlicht in: | International journal of molecular sciences 2020-02, Vol.21 (4) |
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| creator | Verna, Giulio Liso, Marina De Santis, Stefania Dicarlo, Manuela Cavalcanti, Elisabetta Crovace, Alberto Sila, Annamaria Campiglia, Pietro Santino, Angelo Lippolis, Antonio Serino, Grazia Fasano, Alessio Chieppa, Marcello |
| description | Iron overload is an undesired effect of frequent blood transfusions or genetic diseases. Myelodysplastic syndrome (MDS) patients become transfusion dependent, but due to the combination of ineffective haematopoiesis and repeated blood transfusions they are often subject to iron overload. In this study, we demonstrate that iron-overload mimicking condition alters bone marrow progenitor differentiation towards dendritic cells (DCs). Cells cultured in iron-enriched culture medium for seven days fail to differentiate into conventional CD11c
MHCII
DCs and fail to efficiently respond to LPS (Lipopolysaccharides). Cells appear smaller than control DCs but vital and able to perform FITC-dextran (Fluorescein isothiocyanate-dextran) endocytosis. At molecular level, cells cultured in iron-enriched conditions show increased
and
, and decreased
expression. |
| format | Article |
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MHCII
DCs and fail to efficiently respond to LPS (Lipopolysaccharides). Cells appear smaller than control DCs but vital and able to perform FITC-dextran (Fluorescein isothiocyanate-dextran) endocytosis. At molecular level, cells cultured in iron-enriched conditions show increased
and
, and decreased
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MHCII
DCs and fail to efficiently respond to LPS (Lipopolysaccharides). Cells appear smaller than control DCs but vital and able to perform FITC-dextran (Fluorescein isothiocyanate-dextran) endocytosis. At molecular level, cells cultured in iron-enriched conditions show increased
and
, and decreased
expression.</description><subject>Animals</subject><subject>Arginase - genetics</subject><subject>Arginase - metabolism</subject><subject>Bone Marrow - drug effects</subject><subject>Bone Marrow - metabolism</subject><subject>Bone Marrow Cells - metabolism</subject><subject>CD11c Antigen - metabolism</subject><subject>Cell Differentiation - physiology</subject><subject>Cytokines - metabolism</subject><subject>Dendritic Cells - drug effects</subject><subject>Dendritic Cells - metabolism</subject><subject>Gene Expression Regulation</subject><subject>Hematopoiesis</subject><subject>Histocompatibility Antigens Class II - metabolism</subject><subject>Inflammation</subject><subject>Interferon Regulatory Factors - genetics</subject><subject>Interferon Regulatory Factors - metabolism</subject><subject>Iron Overload - metabolism</subject><subject>Lipopolysaccharides - adverse effects</subject><subject>Mice</subject><subject>Proto-Oncogene Proteins - genetics</subject><subject>Proto-Oncogene Proteins - metabolism</subject><subject>Toll-Like Receptor 4 - genetics</subject><subject>Toll-Like Receptor 4 - metabolism</subject><subject>Trans-Activators - metabolism</subject><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFjkuLwjAUhYMg42P8C3L3g5im9bWdVLGLMotxL7G9latpIklVXPrPbUXXrj4O5ztwWqwbREKMOJ_OOqzn_YFzEYrJ4ot1QsFni5BHXXZPnDXwd0GnrcohpZKyI5k9SGtyqsgaD_9HvHr4tQYhVc7ZK8Rocle3GUjU2kNMRYEOTUWqmQCZykK6lkkCutZlHAQZ_Dy5q7mKxnPe5Gb8zdqF0h4HL_bZcLXcyPXodN6VmG9Pjkrlbtv35_Cj8ABu2EnF</recordid><startdate>20200217</startdate><enddate>20200217</enddate><creator>Verna, Giulio</creator><creator>Liso, Marina</creator><creator>De Santis, Stefania</creator><creator>Dicarlo, Manuela</creator><creator>Cavalcanti, Elisabetta</creator><creator>Crovace, Alberto</creator><creator>Sila, Annamaria</creator><creator>Campiglia, Pietro</creator><creator>Santino, Angelo</creator><creator>Lippolis, Antonio</creator><creator>Serino, Grazia</creator><creator>Fasano, Alessio</creator><creator>Chieppa, Marcello</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>20200217</creationdate><title>Iron Overload Mimicking Conditions Skews Bone Marrow Dendritic Cells Differentiation into MHCII low CD11c + CD11b + F4/80 + Cells</title><author>Verna, Giulio ; 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Myelodysplastic syndrome (MDS) patients become transfusion dependent, but due to the combination of ineffective haematopoiesis and repeated blood transfusions they are often subject to iron overload. In this study, we demonstrate that iron-overload mimicking condition alters bone marrow progenitor differentiation towards dendritic cells (DCs). Cells cultured in iron-enriched culture medium for seven days fail to differentiate into conventional CD11c
MHCII
DCs and fail to efficiently respond to LPS (Lipopolysaccharides). Cells appear smaller than control DCs but vital and able to perform FITC-dextran (Fluorescein isothiocyanate-dextran) endocytosis. At molecular level, cells cultured in iron-enriched conditions show increased
and
, and decreased
expression.</abstract><cop>Switzerland</cop><pmid>32079304</pmid></addata></record> |
| fulltext | fulltext |
| identifier | EISSN: 1422-0067 |
| ispartof | International journal of molecular sciences, 2020-02, Vol.21 (4) |
| issn | 1422-0067 |
| language | eng |
| recordid | cdi_pubmed_primary_32079304 |
| source | MEDLINE; MDPI - Multidisciplinary Digital Publishing Institute; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Animals Arginase - genetics Arginase - metabolism Bone Marrow - drug effects Bone Marrow - metabolism Bone Marrow Cells - metabolism CD11c Antigen - metabolism Cell Differentiation - physiology Cytokines - metabolism Dendritic Cells - drug effects Dendritic Cells - metabolism Gene Expression Regulation Hematopoiesis Histocompatibility Antigens Class II - metabolism Inflammation Interferon Regulatory Factors - genetics Interferon Regulatory Factors - metabolism Iron Overload - metabolism Lipopolysaccharides - adverse effects Mice Proto-Oncogene Proteins - genetics Proto-Oncogene Proteins - metabolism Toll-Like Receptor 4 - genetics Toll-Like Receptor 4 - metabolism Trans-Activators - metabolism |
| title | Iron Overload Mimicking Conditions Skews Bone Marrow Dendritic Cells Differentiation into MHCII low CD11c + CD11b + F4/80 + Cells |
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