RT-QuIC-based detection of alpha-synuclein seeding activity in brains of dementia with Lewy Body patients and of a transgenic mouse model of synucleinopathy
RT-QuIC is a shaking-based cyclic amplification technique originally developed in the prion field to detect minute amounts of scrapie prion protein (PrP Sc ). In this study, we applied the RT-QuIC assay to investigate a-synuclein (a-syn) seeding activity in brains of Dementia with Lewy Body (DLB) pa...
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| Veröffentlicht in: | Prion 2020-01, Vol.14 (1), p.88-94 |
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| creator | Han, Jung-Youn Jang, Hyung-Sup Green, Alison J. E. Choi, Young Pyo |
| description | RT-QuIC is a shaking-based cyclic amplification technique originally developed in the prion field to detect minute amounts of scrapie prion protein (PrP
Sc
). In this study, we applied the RT-QuIC assay to investigate a-synuclein (a-syn) seeding activity in brains of Dementia with Lewy Body (DLB) patients and in brains of G2-3 transgenic mice expressing human a-syn with A53T mutation. The results show that a-syn seeding activity varies between patients with detectable dilutions ranging from 10
−3
to 10
−8
dilutions of brain tissue and is stable under exposures to the cycles of freezing, thawing and sonication. A53T a-syn aggregates from G2-3 transgenic mice greatly favoured A53T recombinant human a-syn as substrates in comparison to wild-type a-syn, suggesting that conformations for wild-type a-syn to be able to adopt are not compatible with that of A53T aggregates from G2-3. |
| doi_str_mv | 10.1080/19336896.2020.1724608 |
| format | Article |
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Sc
). In this study, we applied the RT-QuIC assay to investigate a-synuclein (a-syn) seeding activity in brains of Dementia with Lewy Body (DLB) patients and in brains of G2-3 transgenic mice expressing human a-syn with A53T mutation. The results show that a-syn seeding activity varies between patients with detectable dilutions ranging from 10
−3
to 10
−8
dilutions of brain tissue and is stable under exposures to the cycles of freezing, thawing and sonication. A53T a-syn aggregates from G2-3 transgenic mice greatly favoured A53T recombinant human a-syn as substrates in comparison to wild-type a-syn, suggesting that conformations for wild-type a-syn to be able to adopt are not compatible with that of A53T aggregates from G2-3.</description><identifier>ISSN: 1933-6896</identifier><identifier>EISSN: 1933-690X</identifier><identifier>DOI: 10.1080/19336896.2020.1724608</identifier><identifier>PMID: 32041499</identifier><language>eng</language><publisher>United States: Taylor & Francis</publisher><subject>a-synuclein ; RT-QuIC ; seeded aggregation ; Short Communication</subject><ispartof>Prion, 2020-01, Vol.14 (1), p.88-94</ispartof><rights>2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. 2020</rights><rights>2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. 2020 The Author(s)</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c468t-91f03470cb575b68c6950f6b646357429d18d6e6118c2033d3e353acf71080c23</citedby><cites>FETCH-LOGICAL-c468t-91f03470cb575b68c6950f6b646357429d18d6e6118c2033d3e353acf71080c23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7039666/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7039666/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27502,27924,27925,53791,53793,59143,59144</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32041499$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Han, Jung-Youn</creatorcontrib><creatorcontrib>Jang, Hyung-Sup</creatorcontrib><creatorcontrib>Green, Alison J. E.</creatorcontrib><creatorcontrib>Choi, Young Pyo</creatorcontrib><title>RT-QuIC-based detection of alpha-synuclein seeding activity in brains of dementia with Lewy Body patients and of a transgenic mouse model of synucleinopathy</title><title>Prion</title><addtitle>Prion</addtitle><description>RT-QuIC is a shaking-based cyclic amplification technique originally developed in the prion field to detect minute amounts of scrapie prion protein (PrP
Sc
). In this study, we applied the RT-QuIC assay to investigate a-synuclein (a-syn) seeding activity in brains of Dementia with Lewy Body (DLB) patients and in brains of G2-3 transgenic mice expressing human a-syn with A53T mutation. The results show that a-syn seeding activity varies between patients with detectable dilutions ranging from 10
−3
to 10
−8
dilutions of brain tissue and is stable under exposures to the cycles of freezing, thawing and sonication. A53T a-syn aggregates from G2-3 transgenic mice greatly favoured A53T recombinant human a-syn as substrates in comparison to wild-type a-syn, suggesting that conformations for wild-type a-syn to be able to adopt are not compatible with that of A53T aggregates from G2-3.</description><subject>a-synuclein</subject><subject>RT-QuIC</subject><subject>seeded aggregation</subject><subject>Short Communication</subject><issn>1933-6896</issn><issn>1933-690X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>0YH</sourceid><recordid>eNp9UcuO1DAQjBCIfcAngHzkksWPxIkvCBjBstJICLRI3CzH7swYJfZgOzvKv_CxOMxDcOFiW9XVVe2uonhB8A3BLX5NBGO8FfyGYpqhhlYct4-KywUvucDfH5_emXRRXMX4A-NaUMqeFheM4opUQlwWv77el1-mu1XZqQgGGUigk_UO-R6pYbdVZZzdpAewDkUAY90Gqcx4sGlGGeuCsi4ubAMjuGQV2tu0RWvYz-i9NzPaqWRzISLlzB9VlIJycQPOajT6KUI-DQxL7ezlc9d2flY86dUQ4fnxvi6-ffxwv_pUrj_f3q3erUtd8TaVgvSYVQ3WXd3UHW81FzXueccrzuqmosKQ1nDghLSaYsYMA1Yzpftm2aSm7Lp4c9DdTd0IRudxgxrkLthRhVl6ZeW_FWe3cuMfZIOZ4JxngVdHgeB_ThCTHG3UMAzKQf6hpNmvblrRLtT6QNXBxxigP9sQLJd55ClZuSQrj8nmvpd_z3juOkWZCW8PBOt6H0a192EwMql58KHPG9c2SvZ_j9-1pLXC</recordid><startdate>20200101</startdate><enddate>20200101</enddate><creator>Han, Jung-Youn</creator><creator>Jang, Hyung-Sup</creator><creator>Green, Alison J. E.</creator><creator>Choi, Young Pyo</creator><general>Taylor & Francis</general><scope>0YH</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20200101</creationdate><title>RT-QuIC-based detection of alpha-synuclein seeding activity in brains of dementia with Lewy Body patients and of a transgenic mouse model of synucleinopathy</title><author>Han, Jung-Youn ; Jang, Hyung-Sup ; Green, Alison J. E. ; Choi, Young Pyo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c468t-91f03470cb575b68c6950f6b646357429d18d6e6118c2033d3e353acf71080c23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>a-synuclein</topic><topic>RT-QuIC</topic><topic>seeded aggregation</topic><topic>Short Communication</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Han, Jung-Youn</creatorcontrib><creatorcontrib>Jang, Hyung-Sup</creatorcontrib><creatorcontrib>Green, Alison J. E.</creatorcontrib><creatorcontrib>Choi, Young Pyo</creatorcontrib><collection>Taylor & Francis Open Access</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Prion</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Han, Jung-Youn</au><au>Jang, Hyung-Sup</au><au>Green, Alison J. E.</au><au>Choi, Young Pyo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>RT-QuIC-based detection of alpha-synuclein seeding activity in brains of dementia with Lewy Body patients and of a transgenic mouse model of synucleinopathy</atitle><jtitle>Prion</jtitle><addtitle>Prion</addtitle><date>2020-01-01</date><risdate>2020</risdate><volume>14</volume><issue>1</issue><spage>88</spage><epage>94</epage><pages>88-94</pages><issn>1933-6896</issn><eissn>1933-690X</eissn><abstract>RT-QuIC is a shaking-based cyclic amplification technique originally developed in the prion field to detect minute amounts of scrapie prion protein (PrP
Sc
). In this study, we applied the RT-QuIC assay to investigate a-synuclein (a-syn) seeding activity in brains of Dementia with Lewy Body (DLB) patients and in brains of G2-3 transgenic mice expressing human a-syn with A53T mutation. The results show that a-syn seeding activity varies between patients with detectable dilutions ranging from 10
−3
to 10
−8
dilutions of brain tissue and is stable under exposures to the cycles of freezing, thawing and sonication. A53T a-syn aggregates from G2-3 transgenic mice greatly favoured A53T recombinant human a-syn as substrates in comparison to wild-type a-syn, suggesting that conformations for wild-type a-syn to be able to adopt are not compatible with that of A53T aggregates from G2-3.</abstract><cop>United States</cop><pub>Taylor & Francis</pub><pmid>32041499</pmid><doi>10.1080/19336896.2020.1724608</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | Taylor & Francis Open Access; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | a-synuclein RT-QuIC seeded aggregation Short Communication |
| title | RT-QuIC-based detection of alpha-synuclein seeding activity in brains of dementia with Lewy Body patients and of a transgenic mouse model of synucleinopathy |
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