Shear stress activates ATOH8 via autocrine VEGF promoting glycolysis dependent-survival of colorectal cancer cells in the circulation
Metastasis and recurrence, wherein circulating tumour cells (CTCs) play an important role, are the leading causes of death in colorectal cancer (CRC). Metastasis-initiating CTCs manage to maintain intravascular survival under anoikis, immune attack, and importantly shear stress; however, the underly...
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| Veröffentlicht in: | Journal of experimental & clinical cancer research 2020-01, Vol.39 (1), p.25-16, Article 25 |
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| creator | Huang, Qiong Li, Shaowei Hu, Xingbin Sun, Mengting Wu, Qijing Dai, Huiru Tan, Yujing Sun, Fei Wang, Chunlin Rong, Xiaoxiang Liao, Wangjun Peng, Jianjun Xiao, Jianjun Huang, Li Wang, Jiao Liang, Bishan Lin, Kelin Liu, Yajing Shi, Min |
| description | Metastasis and recurrence, wherein circulating tumour cells (CTCs) play an important role, are the leading causes of death in colorectal cancer (CRC). Metastasis-initiating CTCs manage to maintain intravascular survival under anoikis, immune attack, and importantly shear stress; however, the underlying mechanisms remain poorly understood.
In view of the scarcity of CTCs in the bloodstream, suspended colorectal cancer cells were flowed into the cyclic laminar shear stress (LSS) according to previous studies. Then, we detected these suspended cells with a CK8+/CD45-/DAPI+ phenotype and named them mimic circulating tumour cells (m-CTCs) for subsequent CTCs related researches. Quantitative polymerase chain reaction, western blotting, and immunofluorescence were utilised to analyse gene expression change of m-CTCs sensitive to LSS stimulation. Additionally, we examined atonal bHLH transcription factor 8 (ATOH8) expressions in CTCs among 156 CRC patients and mice by fluorescence in situ hybridisation and flow cytometry. The pro-metabolic and pro-survival functions of ATOH8 were determined by glycolysis assay, live/dead cell vitality assay, anoikis assay, and immunohistochemistry. Further, the concrete up-and-down mechanisms of m-CTC survival promotion by ATOH8 were explored.
The m-CTCs actively responded to LSS by triggering the expression of ATOH8, a fluid mechanosensor, with executive roles in intravascular survival and metabolism plasticity. Specifically, ATOH8 was upregulated via activation of VEGFR2/AKT signalling pathway mediated by LSS induced VEGF release. ATOH8 then transcriptionally activated HK2-mediated glycolysis, thus promoting the intravascular survival of colorectal cancer cells in the circulation.
This study elucidates a novel mechanism that an LSS triggered VEGF-VEGFR2-AKT-ATOH8 signal axis mediates m-CTCs survival, thus providing a potential target for the prevention and treatment of hematogenous metastasis in CRC. |
| doi_str_mv | 10.1186/s13046-020-1533-0 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_doaj_</sourceid><recordid>TN_cdi_pubmed_primary_32000836</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A616355205</galeid><doaj_id>oai_doaj_org_article_e030d038f8fa4a329afd7500b6cc5b8a</doaj_id><sourcerecordid>A616355205</sourcerecordid><originalsourceid>FETCH-LOGICAL-c591t-bebecea09903e4c4995855b5a8835bca32df423fb819848c24fe291378ec6c8a3</originalsourceid><addsrcrecordid>eNptks9qGzEQxpfS0qRuH6CXIij0tqm0WsnSpWBC_kEgh6a9Cu3s7FpmvXIlrcEP0PeuHKepDUUHSTPf_NCMvqL4yOgFY0p-jYzTWpa0oiUTnJf0VXHO5kKWWkv5-uh8VryLcUWpZJrpt8UZryilisvz4vf3JdpAYgoYI7GQ3NYmjGTx-HCryNZZYqfkIbgRyc-rm2uyCX7tkxt70g878MMuukha3ODY4pjKOIVtRgzEdyRnfUBI-QZ2BAwEcBgicSNJSyTgAkyDTc6P74s3nR0ifnjeZ8WP66vHy9vy_uHm7nJxX4LQLJUNNghoqdaUYw211kIJ0QirFBcNWF61XV3xrlFMq1pBVXdYacbnCkGCsnxW3B24rbcrswlubcPOeOvMU8CH3tiQHAxokHLaUq461dk6k7Xt2rmgtJEAonlifTuwNlOzxhZy98EOJ9DTzOiWpvdbI7XmdZ7-rPj8DAj-14QxmZWfwpj7NxUX80rPKav_qXqbX-XGzmcYrF0Es5BMciEqKrLq4j-qvFpcO_Ajdi7HTwq-HBVkDwxpGf0w7X8jngrZQQjBxxiwe-mQUbN3oTm40GQXmr0LDc01n45H81Lx13b8DyVi2ME</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2357297014</pqid></control><display><type>article</type><title>Shear stress activates ATOH8 via autocrine VEGF promoting glycolysis dependent-survival of colorectal cancer cells in the circulation</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>PubMed Central Open Access</source><source>Springer Nature OA Free Journals</source><creator>Huang, Qiong ; Li, Shaowei ; Hu, Xingbin ; Sun, Mengting ; Wu, Qijing ; Dai, Huiru ; Tan, Yujing ; Sun, Fei ; Wang, Chunlin ; Rong, Xiaoxiang ; Liao, Wangjun ; Peng, Jianjun ; Xiao, Jianjun ; Huang, Li ; Wang, Jiao ; Liang, Bishan ; Lin, Kelin ; Liu, Yajing ; Shi, Min</creator><creatorcontrib>Huang, Qiong ; Li, Shaowei ; Hu, Xingbin ; Sun, Mengting ; Wu, Qijing ; Dai, Huiru ; Tan, Yujing ; Sun, Fei ; Wang, Chunlin ; Rong, Xiaoxiang ; Liao, Wangjun ; Peng, Jianjun ; Xiao, Jianjun ; Huang, Li ; Wang, Jiao ; Liang, Bishan ; Lin, Kelin ; Liu, Yajing ; Shi, Min</creatorcontrib><description>Metastasis and recurrence, wherein circulating tumour cells (CTCs) play an important role, are the leading causes of death in colorectal cancer (CRC). Metastasis-initiating CTCs manage to maintain intravascular survival under anoikis, immune attack, and importantly shear stress; however, the underlying mechanisms remain poorly understood.
In view of the scarcity of CTCs in the bloodstream, suspended colorectal cancer cells were flowed into the cyclic laminar shear stress (LSS) according to previous studies. Then, we detected these suspended cells with a CK8+/CD45-/DAPI+ phenotype and named them mimic circulating tumour cells (m-CTCs) for subsequent CTCs related researches. Quantitative polymerase chain reaction, western blotting, and immunofluorescence were utilised to analyse gene expression change of m-CTCs sensitive to LSS stimulation. Additionally, we examined atonal bHLH transcription factor 8 (ATOH8) expressions in CTCs among 156 CRC patients and mice by fluorescence in situ hybridisation and flow cytometry. The pro-metabolic and pro-survival functions of ATOH8 were determined by glycolysis assay, live/dead cell vitality assay, anoikis assay, and immunohistochemistry. Further, the concrete up-and-down mechanisms of m-CTC survival promotion by ATOH8 were explored.
The m-CTCs actively responded to LSS by triggering the expression of ATOH8, a fluid mechanosensor, with executive roles in intravascular survival and metabolism plasticity. Specifically, ATOH8 was upregulated via activation of VEGFR2/AKT signalling pathway mediated by LSS induced VEGF release. ATOH8 then transcriptionally activated HK2-mediated glycolysis, thus promoting the intravascular survival of colorectal cancer cells in the circulation.
This study elucidates a novel mechanism that an LSS triggered VEGF-VEGFR2-AKT-ATOH8 signal axis mediates m-CTCs survival, thus providing a potential target for the prevention and treatment of hematogenous metastasis in CRC.</description><identifier>ISSN: 1756-9966</identifier><identifier>ISSN: 0392-9078</identifier><identifier>EISSN: 1756-9966</identifier><identifier>DOI: 10.1186/s13046-020-1533-0</identifier><identifier>PMID: 32000836</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Animals ; Apoptosis ; ATOH8 ; Basic Helix-Loop-Helix Transcription Factors - metabolism ; Cancer cells ; Cancer metastasis ; Cancer research ; Cell Line, Tumor ; Cell Survival - physiology ; Cohort Studies ; Colorectal cancer ; Colorectal Neoplasms - blood ; Colorectal Neoplasms - metabolism ; Colorectal Neoplasms - pathology ; Death ; Endothelial growth factors ; Experiments ; Female ; Flow velocity ; Fluorescence ; Fluorescent antibody technique ; Gene expression ; Genes ; Genotype & phenotype ; Glucose metabolism ; Glycolysis ; HCT116 Cells ; HT29 Cells ; Humans ; Hypertension ; Immunohistochemistry ; Laboratory animals ; Laminar shear stress ; Liver cancer ; Medical prognosis ; Medical research ; Metastasis ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Neoplasm Metastasis ; Neoplastic Cells, Circulating - metabolism ; Neoplastic Cells, Circulating - pathology ; Novels ; Physiological aspects ; Physiology ; Polymerase chain reaction ; Recurrence (Disease) ; Shear Strength ; Shear stress ; Software ; Stem cells ; Tumors ; Vascular endothelial growth factor ; Vascular Endothelial Growth Factor A - metabolism ; VEGF</subject><ispartof>Journal of experimental & clinical cancer research, 2020-01, Vol.39 (1), p.25-16, Article 25</ispartof><rights>COPYRIGHT 2020 BioMed Central Ltd.</rights><rights>2020. This work is licensed under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>The Author(s). 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c591t-bebecea09903e4c4995855b5a8835bca32df423fb819848c24fe291378ec6c8a3</citedby><cites>FETCH-LOGICAL-c591t-bebecea09903e4c4995855b5a8835bca32df423fb819848c24fe291378ec6c8a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6993408/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6993408/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32000836$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Huang, Qiong</creatorcontrib><creatorcontrib>Li, Shaowei</creatorcontrib><creatorcontrib>Hu, Xingbin</creatorcontrib><creatorcontrib>Sun, Mengting</creatorcontrib><creatorcontrib>Wu, Qijing</creatorcontrib><creatorcontrib>Dai, Huiru</creatorcontrib><creatorcontrib>Tan, Yujing</creatorcontrib><creatorcontrib>Sun, Fei</creatorcontrib><creatorcontrib>Wang, Chunlin</creatorcontrib><creatorcontrib>Rong, Xiaoxiang</creatorcontrib><creatorcontrib>Liao, Wangjun</creatorcontrib><creatorcontrib>Peng, Jianjun</creatorcontrib><creatorcontrib>Xiao, Jianjun</creatorcontrib><creatorcontrib>Huang, Li</creatorcontrib><creatorcontrib>Wang, Jiao</creatorcontrib><creatorcontrib>Liang, Bishan</creatorcontrib><creatorcontrib>Lin, Kelin</creatorcontrib><creatorcontrib>Liu, Yajing</creatorcontrib><creatorcontrib>Shi, Min</creatorcontrib><title>Shear stress activates ATOH8 via autocrine VEGF promoting glycolysis dependent-survival of colorectal cancer cells in the circulation</title><title>Journal of experimental & clinical cancer research</title><addtitle>J Exp Clin Cancer Res</addtitle><description>Metastasis and recurrence, wherein circulating tumour cells (CTCs) play an important role, are the leading causes of death in colorectal cancer (CRC). Metastasis-initiating CTCs manage to maintain intravascular survival under anoikis, immune attack, and importantly shear stress; however, the underlying mechanisms remain poorly understood.
In view of the scarcity of CTCs in the bloodstream, suspended colorectal cancer cells were flowed into the cyclic laminar shear stress (LSS) according to previous studies. Then, we detected these suspended cells with a CK8+/CD45-/DAPI+ phenotype and named them mimic circulating tumour cells (m-CTCs) for subsequent CTCs related researches. Quantitative polymerase chain reaction, western blotting, and immunofluorescence were utilised to analyse gene expression change of m-CTCs sensitive to LSS stimulation. Additionally, we examined atonal bHLH transcription factor 8 (ATOH8) expressions in CTCs among 156 CRC patients and mice by fluorescence in situ hybridisation and flow cytometry. The pro-metabolic and pro-survival functions of ATOH8 were determined by glycolysis assay, live/dead cell vitality assay, anoikis assay, and immunohistochemistry. Further, the concrete up-and-down mechanisms of m-CTC survival promotion by ATOH8 were explored.
The m-CTCs actively responded to LSS by triggering the expression of ATOH8, a fluid mechanosensor, with executive roles in intravascular survival and metabolism plasticity. Specifically, ATOH8 was upregulated via activation of VEGFR2/AKT signalling pathway mediated by LSS induced VEGF release. ATOH8 then transcriptionally activated HK2-mediated glycolysis, thus promoting the intravascular survival of colorectal cancer cells in the circulation.
This study elucidates a novel mechanism that an LSS triggered VEGF-VEGFR2-AKT-ATOH8 signal axis mediates m-CTCs survival, thus providing a potential target for the prevention and treatment of hematogenous metastasis in CRC.</description><subject>Animals</subject><subject>Apoptosis</subject><subject>ATOH8</subject><subject>Basic Helix-Loop-Helix Transcription Factors - metabolism</subject><subject>Cancer cells</subject><subject>Cancer metastasis</subject><subject>Cancer research</subject><subject>Cell Line, Tumor</subject><subject>Cell Survival - physiology</subject><subject>Cohort Studies</subject><subject>Colorectal cancer</subject><subject>Colorectal Neoplasms - blood</subject><subject>Colorectal Neoplasms - metabolism</subject><subject>Colorectal Neoplasms - pathology</subject><subject>Death</subject><subject>Endothelial growth factors</subject><subject>Experiments</subject><subject>Female</subject><subject>Flow velocity</subject><subject>Fluorescence</subject><subject>Fluorescent antibody technique</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Genotype & phenotype</subject><subject>Glucose metabolism</subject><subject>Glycolysis</subject><subject>HCT116 Cells</subject><subject>HT29 Cells</subject><subject>Humans</subject><subject>Hypertension</subject><subject>Immunohistochemistry</subject><subject>Laboratory animals</subject><subject>Laminar shear stress</subject><subject>Liver cancer</subject><subject>Medical prognosis</subject><subject>Medical research</subject><subject>Metastasis</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Nude</subject><subject>Neoplasm Metastasis</subject><subject>Neoplastic Cells, Circulating - metabolism</subject><subject>Neoplastic Cells, Circulating - pathology</subject><subject>Novels</subject><subject>Physiological aspects</subject><subject>Physiology</subject><subject>Polymerase chain reaction</subject><subject>Recurrence (Disease)</subject><subject>Shear Strength</subject><subject>Shear stress</subject><subject>Software</subject><subject>Stem cells</subject><subject>Tumors</subject><subject>Vascular endothelial growth factor</subject><subject>Vascular Endothelial Growth Factor A - metabolism</subject><subject>VEGF</subject><issn>1756-9966</issn><issn>0392-9078</issn><issn>1756-9966</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNptks9qGzEQxpfS0qRuH6CXIij0tqm0WsnSpWBC_kEgh6a9Cu3s7FpmvXIlrcEP0PeuHKepDUUHSTPf_NCMvqL4yOgFY0p-jYzTWpa0oiUTnJf0VXHO5kKWWkv5-uh8VryLcUWpZJrpt8UZryilisvz4vf3JdpAYgoYI7GQ3NYmjGTx-HCryNZZYqfkIbgRyc-rm2uyCX7tkxt70g878MMuukha3ODY4pjKOIVtRgzEdyRnfUBI-QZ2BAwEcBgicSNJSyTgAkyDTc6P74s3nR0ifnjeZ8WP66vHy9vy_uHm7nJxX4LQLJUNNghoqdaUYw211kIJ0QirFBcNWF61XV3xrlFMq1pBVXdYacbnCkGCsnxW3B24rbcrswlubcPOeOvMU8CH3tiQHAxokHLaUq461dk6k7Xt2rmgtJEAonlifTuwNlOzxhZy98EOJ9DTzOiWpvdbI7XmdZ7-rPj8DAj-14QxmZWfwpj7NxUX80rPKav_qXqbX-XGzmcYrF0Es5BMciEqKrLq4j-qvFpcO_Ajdi7HTwq-HBVkDwxpGf0w7X8jngrZQQjBxxiwe-mQUbN3oTm40GQXmr0LDc01n45H81Lx13b8DyVi2ME</recordid><startdate>20200130</startdate><enddate>20200130</enddate><creator>Huang, Qiong</creator><creator>Li, Shaowei</creator><creator>Hu, Xingbin</creator><creator>Sun, Mengting</creator><creator>Wu, Qijing</creator><creator>Dai, Huiru</creator><creator>Tan, Yujing</creator><creator>Sun, Fei</creator><creator>Wang, Chunlin</creator><creator>Rong, Xiaoxiang</creator><creator>Liao, Wangjun</creator><creator>Peng, Jianjun</creator><creator>Xiao, Jianjun</creator><creator>Huang, Li</creator><creator>Wang, Jiao</creator><creator>Liang, Bishan</creator><creator>Lin, Kelin</creator><creator>Liu, Yajing</creator><creator>Shi, Min</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><general>BMC</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PIMPY</scope><scope>PJZUB</scope><scope>PKEHL</scope><scope>PPXIY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20200130</creationdate><title>Shear stress activates ATOH8 via autocrine VEGF promoting glycolysis dependent-survival of colorectal cancer cells in the circulation</title><author>Huang, Qiong ; Li, Shaowei ; Hu, Xingbin ; Sun, Mengting ; Wu, Qijing ; Dai, Huiru ; Tan, Yujing ; Sun, Fei ; Wang, Chunlin ; Rong, Xiaoxiang ; Liao, Wangjun ; Peng, Jianjun ; Xiao, Jianjun ; Huang, Li ; Wang, Jiao ; Liang, Bishan ; Lin, Kelin ; Liu, Yajing ; Shi, Min</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c591t-bebecea09903e4c4995855b5a8835bca32df423fb819848c24fe291378ec6c8a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Animals</topic><topic>Apoptosis</topic><topic>ATOH8</topic><topic>Basic Helix-Loop-Helix Transcription Factors - metabolism</topic><topic>Cancer cells</topic><topic>Cancer metastasis</topic><topic>Cancer research</topic><topic>Cell Line, Tumor</topic><topic>Cell Survival - physiology</topic><topic>Cohort Studies</topic><topic>Colorectal cancer</topic><topic>Colorectal Neoplasms - blood</topic><topic>Colorectal Neoplasms - metabolism</topic><topic>Colorectal Neoplasms - pathology</topic><topic>Death</topic><topic>Endothelial growth factors</topic><topic>Experiments</topic><topic>Female</topic><topic>Flow velocity</topic><topic>Fluorescence</topic><topic>Fluorescent antibody technique</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Genotype & phenotype</topic><topic>Glucose metabolism</topic><topic>Glycolysis</topic><topic>HCT116 Cells</topic><topic>HT29 Cells</topic><topic>Humans</topic><topic>Hypertension</topic><topic>Immunohistochemistry</topic><topic>Laboratory animals</topic><topic>Laminar shear stress</topic><topic>Liver cancer</topic><topic>Medical prognosis</topic><topic>Medical research</topic><topic>Metastasis</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mice, Nude</topic><topic>Neoplasm Metastasis</topic><topic>Neoplastic Cells, Circulating - 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Metastasis-initiating CTCs manage to maintain intravascular survival under anoikis, immune attack, and importantly shear stress; however, the underlying mechanisms remain poorly understood.
In view of the scarcity of CTCs in the bloodstream, suspended colorectal cancer cells were flowed into the cyclic laminar shear stress (LSS) according to previous studies. Then, we detected these suspended cells with a CK8+/CD45-/DAPI+ phenotype and named them mimic circulating tumour cells (m-CTCs) for subsequent CTCs related researches. Quantitative polymerase chain reaction, western blotting, and immunofluorescence were utilised to analyse gene expression change of m-CTCs sensitive to LSS stimulation. Additionally, we examined atonal bHLH transcription factor 8 (ATOH8) expressions in CTCs among 156 CRC patients and mice by fluorescence in situ hybridisation and flow cytometry. The pro-metabolic and pro-survival functions of ATOH8 were determined by glycolysis assay, live/dead cell vitality assay, anoikis assay, and immunohistochemistry. Further, the concrete up-and-down mechanisms of m-CTC survival promotion by ATOH8 were explored.
The m-CTCs actively responded to LSS by triggering the expression of ATOH8, a fluid mechanosensor, with executive roles in intravascular survival and metabolism plasticity. Specifically, ATOH8 was upregulated via activation of VEGFR2/AKT signalling pathway mediated by LSS induced VEGF release. ATOH8 then transcriptionally activated HK2-mediated glycolysis, thus promoting the intravascular survival of colorectal cancer cells in the circulation.
This study elucidates a novel mechanism that an LSS triggered VEGF-VEGFR2-AKT-ATOH8 signal axis mediates m-CTCs survival, thus providing a potential target for the prevention and treatment of hematogenous metastasis in CRC.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>32000836</pmid><doi>10.1186/s13046-020-1533-0</doi><tpages>16</tpages><oa>free_for_read</oa></addata></record> |
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| recordid | cdi_pubmed_primary_32000836 |
| source | MEDLINE; Springer Nature - Complete Springer Journals; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; PubMed Central Open Access; Springer Nature OA Free Journals |
| subjects | Animals Apoptosis ATOH8 Basic Helix-Loop-Helix Transcription Factors - metabolism Cancer cells Cancer metastasis Cancer research Cell Line, Tumor Cell Survival - physiology Cohort Studies Colorectal cancer Colorectal Neoplasms - blood Colorectal Neoplasms - metabolism Colorectal Neoplasms - pathology Death Endothelial growth factors Experiments Female Flow velocity Fluorescence Fluorescent antibody technique Gene expression Genes Genotype & phenotype Glucose metabolism Glycolysis HCT116 Cells HT29 Cells Humans Hypertension Immunohistochemistry Laboratory animals Laminar shear stress Liver cancer Medical prognosis Medical research Metastasis Mice Mice, Inbred BALB C Mice, Nude Neoplasm Metastasis Neoplastic Cells, Circulating - metabolism Neoplastic Cells, Circulating - pathology Novels Physiological aspects Physiology Polymerase chain reaction Recurrence (Disease) Shear Strength Shear stress Software Stem cells Tumors Vascular endothelial growth factor Vascular Endothelial Growth Factor A - metabolism VEGF |
| title | Shear stress activates ATOH8 via autocrine VEGF promoting glycolysis dependent-survival of colorectal cancer cells in the circulation |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-18T23%3A01%3A48IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Shear%20stress%20activates%20ATOH8%20via%20autocrine%20VEGF%20promoting%20glycolysis%20dependent-survival%20of%20colorectal%20cancer%20cells%20in%20the%20circulation&rft.jtitle=Journal%20of%20experimental%20&%20clinical%20cancer%20research&rft.au=Huang,%20Qiong&rft.date=2020-01-30&rft.volume=39&rft.issue=1&rft.spage=25&rft.epage=16&rft.pages=25-16&rft.artnum=25&rft.issn=1756-9966&rft.eissn=1756-9966&rft_id=info:doi/10.1186/s13046-020-1533-0&rft_dat=%3Cgale_doaj_%3EA616355205%3C/gale_doaj_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2357297014&rft_id=info:pmid/32000836&rft_galeid=A616355205&rft_doaj_id=oai_doaj_org_article_e030d038f8fa4a329afd7500b6cc5b8a&rfr_iscdi=true |