Intra-individual variation of particles in exhaled air and of the contents of Surfactant protein A and albumin
Particles in exhaled air (PEx) provide samples of respiratory tract lining fluid from small airways containing, for example, Surfactant protein A (SP-A) and albumin, potential biomarkers of small airway disease. We hypothesized that there are differences between morning, noon, and afternoon measurem...
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| description | Particles in exhaled air (PEx) provide samples of respiratory tract lining fluid from small airways containing, for example, Surfactant protein A (SP-A) and albumin, potential biomarkers of small airway disease. We hypothesized that there are differences between morning, noon, and afternoon measurements and that the variability of repeated measurements is larger between days than within days.
PEx was obtained in sixteen healthy non-smoking adults on 11 occasions, within one day and between days. SP-A and albumin were quantified by ELISA. The coefficient of repeatability (CR), intraclass correlation coefficient (ICC), and coefficient of variation (CV) were used to assess the variation of repeated measurements.
SP-A and albumin increased significantly from morning towards the noon and afternoon by 13% and 25% on average, respectively, whereas PEx number concentration and particle mean mass did not differ significantly between the morning, noon and afternoon. Between-day CRs were not larger than within-day CRs.
Time of the day influences the contents of SP-A and albumin in exhaled particles. The variation of repeated measurements was rather high but was not influenced by the time intervals between measurements. |
| doi_str_mv | 10.1371/journal.pone.0227980 |
| format | Article |
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PEx was obtained in sixteen healthy non-smoking adults on 11 occasions, within one day and between days. SP-A and albumin were quantified by ELISA. The coefficient of repeatability (CR), intraclass correlation coefficient (ICC), and coefficient of variation (CV) were used to assess the variation of repeated measurements.
SP-A and albumin increased significantly from morning towards the noon and afternoon by 13% and 25% on average, respectively, whereas PEx number concentration and particle mean mass did not differ significantly between the morning, noon and afternoon. Between-day CRs were not larger than within-day CRs.
Time of the day influences the contents of SP-A and albumin in exhaled particles. The variation of repeated measurements was rather high but was not influenced by the time intervals between measurements.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0227980</identifier><identifier>PMID: 31978133</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adult ; Aged ; Air - analysis ; Albumin ; Albumins ; Albumins - isolation & purification ; Albumins - metabolism ; Allergic diseases ; Asthma ; Biological markers ; Biology and Life Sciences ; Biomarkers ; Biomarkers - chemistry ; Breath Tests ; Chronic obstructive pulmonary disease ; Coefficient of variation ; Correlation coefficient ; Correlation coefficients ; Environmental health ; Enzyme-linked immunosorbent assay ; Exhalation - physiology ; Female ; Health care ; Healthy Volunteers ; Humans ; Lungmedicin och allergi ; Male ; Medicine ; Medicine and Health Sciences ; Middle Aged ; Morning ; Normal distribution ; Physical Sciences ; Protein A ; Proteins ; Public health ; Pulmonary Disease, Chronic Obstructive - diagnosis ; Pulmonary Disease, Chronic Obstructive - metabolism ; Pulmonary Disease, Chronic Obstructive - pathology ; Pulmonary Surfactant-Associated Protein A - isolation & purification ; Pulmonary Surfactant-Associated Protein A - metabolism ; Research and Analysis Methods ; Respiratory Medicine and Allergy ; Respiratory System - chemistry ; Respiratory System - metabolism ; Respiratory tract ; Respiratory tract diseases ; Silicon wafers ; Social Sciences ; Spirometry - methods ; Surface active agents ; Surfactant protein A ; Surfactants ; Variation</subject><ispartof>PloS one, 2020-01, Vol.15 (1), p.e0227980</ispartof><rights>COPYRIGHT 2020 Public Library of Science</rights><rights>2020 Kokelj et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2020 Kokelj et al 2020 Kokelj et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c730t-970fa3488730167de350a9f5df17fff53ac3daff471a0c79079a40d938c3f0a3</citedby><cites>FETCH-LOGICAL-c730t-970fa3488730167de350a9f5df17fff53ac3daff471a0c79079a40d938c3f0a3</cites><orcidid>0000-0002-5863-057X ; 0000-0003-0911-3493</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6980535/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6980535/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31978133$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://gup.ub.gu.se/publication/290571$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><contributor>Bhattarai, Ajaya</contributor><creatorcontrib>Kokelj, Spela</creatorcontrib><creatorcontrib>Kim, Jeong-Lim</creatorcontrib><creatorcontrib>Andersson, Marianne</creatorcontrib><creatorcontrib>Runström Eden, Gunilla</creatorcontrib><creatorcontrib>Bake, Björn</creatorcontrib><creatorcontrib>Olin, Anna-Carin</creatorcontrib><title>Intra-individual variation of particles in exhaled air and of the contents of Surfactant protein A and albumin</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Particles in exhaled air (PEx) provide samples of respiratory tract lining fluid from small airways containing, for example, Surfactant protein A (SP-A) and albumin, potential biomarkers of small airway disease. We hypothesized that there are differences between morning, noon, and afternoon measurements and that the variability of repeated measurements is larger between days than within days.
PEx was obtained in sixteen healthy non-smoking adults on 11 occasions, within one day and between days. SP-A and albumin were quantified by ELISA. The coefficient of repeatability (CR), intraclass correlation coefficient (ICC), and coefficient of variation (CV) were used to assess the variation of repeated measurements.
SP-A and albumin increased significantly from morning towards the noon and afternoon by 13% and 25% on average, respectively, whereas PEx number concentration and particle mean mass did not differ significantly between the morning, noon and afternoon. Between-day CRs were not larger than within-day CRs.
Time of the day influences the contents of SP-A and albumin in exhaled particles. The variation of repeated measurements was rather high but was not influenced by the time intervals between measurements.</description><subject>Adult</subject><subject>Aged</subject><subject>Air - analysis</subject><subject>Albumin</subject><subject>Albumins</subject><subject>Albumins - isolation & purification</subject><subject>Albumins - metabolism</subject><subject>Allergic diseases</subject><subject>Asthma</subject><subject>Biological markers</subject><subject>Biology and Life Sciences</subject><subject>Biomarkers</subject><subject>Biomarkers - chemistry</subject><subject>Breath Tests</subject><subject>Chronic obstructive pulmonary disease</subject><subject>Coefficient of variation</subject><subject>Correlation coefficient</subject><subject>Correlation coefficients</subject><subject>Environmental health</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Exhalation - physiology</subject><subject>Female</subject><subject>Health care</subject><subject>Healthy Volunteers</subject><subject>Humans</subject><subject>Lungmedicin och allergi</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine and Health Sciences</subject><subject>Middle Aged</subject><subject>Morning</subject><subject>Normal distribution</subject><subject>Physical Sciences</subject><subject>Protein A</subject><subject>Proteins</subject><subject>Public health</subject><subject>Pulmonary Disease, Chronic Obstructive - diagnosis</subject><subject>Pulmonary Disease, Chronic Obstructive - metabolism</subject><subject>Pulmonary Disease, Chronic Obstructive - pathology</subject><subject>Pulmonary Surfactant-Associated Protein A - isolation & purification</subject><subject>Pulmonary Surfactant-Associated Protein A - metabolism</subject><subject>Research and Analysis Methods</subject><subject>Respiratory Medicine and Allergy</subject><subject>Respiratory System - chemistry</subject><subject>Respiratory System - metabolism</subject><subject>Respiratory tract</subject><subject>Respiratory tract diseases</subject><subject>Silicon wafers</subject><subject>Social Sciences</subject><subject>Spirometry - methods</subject><subject>Surface active agents</subject><subject>Surfactant protein A</subject><subject>Surfactants</subject><subject>Variation</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk1GL1DAUhYso7rr6D0QLguBDx6Rpm-ZFGJZVBxYW3MXXcCdNOhk6SU3Scf33m850lykoSB_a3HznkJzbmyRvMVpgQvHnrR2cgW7RWyMXKM8pq9Gz5BwzkmdVjsjzk--z5JX3W4RKUlfVy-SMYEZrTMh5YlYmOMi0afReNwN06R6chqCtSa1Ke3BBi076VJtU3m-gk00K2qVgmnE_bGQqrAnSBD-ubwenQAQwIe2dDTKqlgcWuvWw0-Z18kJB5-Wb6X2R3H29urv8nl3ffFtdLq8zQQkKGaNIASnqOq5wRRtJSgRMlY3CVClVEhCkAaUKigEJyhBlUKCGkVoQhYBcJO-Ptn1nPZ-S8jwnRVEWMSgaidWRaCxsee_0DtwfbkHzQ8G6lk9X56pCgiEsSiLWhWQYYqqM5nKNSBGPJ6NXdvTyv2U_rGdu7dDzWGoH7iXPGSopjvyX6XTDeicbIccWdDPZfMfoDW_tnlexxSUpo8GHycDZX4P04R9XnKg2do1ro2w0EzvtBV9WOMcVYXT0WvyFik8jdzq2Viod6zPBp5ng0P770MLgPV_d_vh_9ubnnP14wm4kdGHjbTeMv6Kfg8URFM5676R6Sg4jPo7GYxp8HA0-jUaUvTtN_Un0OAvkAeYCChU</recordid><startdate>20200124</startdate><enddate>20200124</enddate><creator>Kokelj, Spela</creator><creator>Kim, Jeong-Lim</creator><creator>Andersson, Marianne</creator><creator>Runström Eden, Gunilla</creator><creator>Bake, Björn</creator><creator>Olin, Anna-Carin</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>5PM</scope><scope>ADTPV</scope><scope>AOWAS</scope><scope>F1U</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-5863-057X</orcidid><orcidid>https://orcid.org/0000-0003-0911-3493</orcidid></search><sort><creationdate>20200124</creationdate><title>Intra-individual variation of particles in exhaled air and of the contents of Surfactant protein A and albumin</title><author>Kokelj, Spela ; Kim, Jeong-Lim ; Andersson, Marianne ; Runström Eden, Gunilla ; Bake, Björn ; Olin, Anna-Carin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c730t-970fa3488730167de350a9f5df17fff53ac3daff471a0c79079a40d938c3f0a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Air - 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diagnosis</topic><topic>Pulmonary Disease, Chronic Obstructive - metabolism</topic><topic>Pulmonary Disease, Chronic Obstructive - pathology</topic><topic>Pulmonary Surfactant-Associated Protein A - isolation & purification</topic><topic>Pulmonary Surfactant-Associated Protein A - metabolism</topic><topic>Research and Analysis Methods</topic><topic>Respiratory Medicine and Allergy</topic><topic>Respiratory System - chemistry</topic><topic>Respiratory System - metabolism</topic><topic>Respiratory tract</topic><topic>Respiratory tract diseases</topic><topic>Silicon wafers</topic><topic>Social Sciences</topic><topic>Spirometry - methods</topic><topic>Surface active agents</topic><topic>Surfactant protein A</topic><topic>Surfactants</topic><topic>Variation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kokelj, Spela</creatorcontrib><creatorcontrib>Kim, Jeong-Lim</creatorcontrib><creatorcontrib>Andersson, Marianne</creatorcontrib><creatorcontrib>Runström Eden, Gunilla</creatorcontrib><creatorcontrib>Bake, Björn</creatorcontrib><creatorcontrib>Olin, Anna-Carin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - 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We hypothesized that there are differences between morning, noon, and afternoon measurements and that the variability of repeated measurements is larger between days than within days.
PEx was obtained in sixteen healthy non-smoking adults on 11 occasions, within one day and between days. SP-A and albumin were quantified by ELISA. The coefficient of repeatability (CR), intraclass correlation coefficient (ICC), and coefficient of variation (CV) were used to assess the variation of repeated measurements.
SP-A and albumin increased significantly from morning towards the noon and afternoon by 13% and 25% on average, respectively, whereas PEx number concentration and particle mean mass did not differ significantly between the morning, noon and afternoon. Between-day CRs were not larger than within-day CRs.
Time of the day influences the contents of SP-A and albumin in exhaled particles. The variation of repeated measurements was rather high but was not influenced by the time intervals between measurements.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>31978133</pmid><doi>10.1371/journal.pone.0227980</doi><tpages>e0227980</tpages><orcidid>https://orcid.org/0000-0002-5863-057X</orcidid><orcidid>https://orcid.org/0000-0003-0911-3493</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2020-01, Vol.15 (1), p.e0227980 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_pubmed_primary_31978133 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Adult Aged Air - analysis Albumin Albumins Albumins - isolation & purification Albumins - metabolism Allergic diseases Asthma Biological markers Biology and Life Sciences Biomarkers Biomarkers - chemistry Breath Tests Chronic obstructive pulmonary disease Coefficient of variation Correlation coefficient Correlation coefficients Environmental health Enzyme-linked immunosorbent assay Exhalation - physiology Female Health care Healthy Volunteers Humans Lungmedicin och allergi Male Medicine Medicine and Health Sciences Middle Aged Morning Normal distribution Physical Sciences Protein A Proteins Public health Pulmonary Disease, Chronic Obstructive - diagnosis Pulmonary Disease, Chronic Obstructive - metabolism Pulmonary Disease, Chronic Obstructive - pathology Pulmonary Surfactant-Associated Protein A - isolation & purification Pulmonary Surfactant-Associated Protein A - metabolism Research and Analysis Methods Respiratory Medicine and Allergy Respiratory System - chemistry Respiratory System - metabolism Respiratory tract Respiratory tract diseases Silicon wafers Social Sciences Spirometry - methods Surface active agents Surfactant protein A Surfactants Variation |
| title | Intra-individual variation of particles in exhaled air and of the contents of Surfactant protein A and albumin |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-05T19%3A52%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Intra-individual%20variation%20of%20particles%20in%20exhaled%20air%20and%20of%20the%20contents%20of%20Surfactant%20protein%20A%20and%20albumin&rft.jtitle=PloS%20one&rft.au=Kokelj,%20Spela&rft.date=2020-01-24&rft.volume=15&rft.issue=1&rft.spage=e0227980&rft.pages=e0227980-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0227980&rft_dat=%3Cgale_plos_%3EA612163975%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2344547987&rft_id=info:pmid/31978133&rft_galeid=A612163975&rft_doaj_id=oai_doaj_org_article_f60c901c53cb4e91a932972eb034301e&rfr_iscdi=true |