Direct observation of the formation of a CRISPR-Cas12a R-loop complex at the single-molecule level

Here, we develop an optical tweezers-based single-molecule manipulation assay to detect the formation of an R-loop complex in the Cas12a system and characterize its thermodynamic stability. We found that the formation of the R-loop complex induces a two-step unfolding of a DNA hairpin containing the...

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Veröffentlicht in:	Chemical communications (Cambridge, England) England), 2020-02, Vol.56 (14), p.2123-2126
Hauptverfasser:	Cui, Yang, Tang, Yangchao, Liang, Meng, Ji, Qinghua, Zeng, Yan, Chen, Hui, Lan, Jie, Jin, Peng, Wang, Lei, Song, Guangtao, Lou, Jizhong
Format:	Artikel
Sprache:	eng
Schlagworte:	CRISPR-Cas Systems - genetics DNA - chemical synthesis DNA - chemistry DNA - genetics Optical Tweezers Thermodynamics
Online-Zugang:	Volltext
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