Eomes transcription factor is required for the development and differentiation of invariant NKT cells

Eomes transcription factor is required for the development and differentiation of invariant NKT cells

Eomes regulates the differentiation of CD8 T cells into effector and memory phases. However, its role in invariant (i)NKT cells remains unknown. Here, we show the impact of Eomes on iNKT cells in the thymus and peripheral tissue using conditional knockout (Eomes-cKO) mice. In the thymus, CD1d-tetram...

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Veröffentlicht in:Communications biology 2019-04, Vol.2 (1), p.150
Hauptverfasser: Shimizu, Kanako, Sato, Yusuke, Kawamura, Masami, Nakazato, Hiroshi, Watanabe, Takashi, Ohara, Osamu, Fujii, Shin-Ichiro
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container_issue 1
container_start_page 150
container_title Communications biology
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creator Shimizu, Kanako
Sato, Yusuke
Kawamura, Masami
Nakazato, Hiroshi
Watanabe, Takashi
Ohara, Osamu
Fujii, Shin-Ichiro
description Eomes regulates the differentiation of CD8 T cells into effector and memory phases. However, its role in invariant (i)NKT cells remains unknown. Here, we show the impact of Eomes on iNKT cells in the thymus and peripheral tissue using conditional knockout (Eomes-cKO) mice. In the thymus, CD1d-tetramer CD24 CD44 NK1.1 CD69 stage 0 iNKT cells express higher levels of Eomes than the other iNKT stages. We also found that Eomes regulates NKT1 cell differentiation predominantly. Interestingly, the expression of Eomes in the steady state is low, but can be upregulated after TCR stimulation. We also showed epigenetic changes in the Eomes locus after activation. In addition, vaccination of C57BL/6, but not Eomes-cKO mice with iNKT ligand-loaded dendritic cells generated KLRG1 iNKT cells in lung, characterized as effector memory phenotype by transcriptome profiling. Thus, Eomes regulates not only the differentiation of NKT1 cells in the thymus, but also their differentiation into memory-like KLRG1 iNKT cells in the periphery.
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title Eomes transcription factor is required for the development and differentiation of invariant NKT cells
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