Dextran-based tube-guides for the regeneration of the rat sciatic nerve after neurotmesis injury
In this work, dextran-based nerve tube-guides were prepared, characterized and used in a standardized animal model of neurotmesis injury. Non-porous and porous transparent tube-guides were obtained by photocrosslinking of two co-macromonomers based on dextran and poly( -caprolactone) (PCL). Swelling...
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| Veröffentlicht in: | Biomaterials science 2020-02, Vol.8 (3), p.798-811 |
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| creator | Pinho, Ana Catarina Vieira Branquinho, Mariana Alvites, Rui Damásio Fonseca, Ana Clotilde Caseiro, Ana Rita Santos Pedrosa, Sílvia Luís, Ana Lúcia Pires, Isabel Prada, Justina Muratori, Luísa Ronchi, Giulia Geuna, Stefano Santos, José Domingos Maurício, Ana Colette Serra, Arménio Coimbra Coelho, Jorge Fernando Jordão |
| description | In this work, dextran-based nerve tube-guides were prepared, characterized and used in a standardized animal model of neurotmesis injury. Non-porous and porous transparent tube-guides were obtained by photocrosslinking of two co-macromonomers based on dextran and poly( -caprolactone) (PCL). Swelling capacity of the tube-guides ranged from 40-60% with no visible constriction of their inner diameter.
In vitro
hydrolytic degradation tests showed that the tube-guides maintained their structural integrity up to 6 months. The
in vivo
performance of the tube-guides was evaluated by entubulation of the rat sciatic nerve after a neurotmesis injury, with a 10 mm-gap between the nerve stumps. The results showed that the tube-guides were able to promote the regeneration of the nerve in a similar manner to what was observed with conventional techniques (nerve graft and end-to-end suture). Stereological analysis proved that nerve regeneration occurred, and both tube-guides presented fibre diameter and g-ratio closer to healthy sciatic nerves. The histomorphometric analysis of
Tibialis anterior
(TA) skeletal muscle showed decreased neurogenic atrophy in the porous tube-guides treated group, presenting measurements that are similar to the uninjured control.
Nerve tube-guides based on dextran and poly( -caprolactone) were successfully used in the recovery of a neurotmesis injury in rat model. |
| doi_str_mv | 10.1039/c9bm00901a |
| format | Article |
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In vitro
hydrolytic degradation tests showed that the tube-guides maintained their structural integrity up to 6 months. The
in vivo
performance of the tube-guides was evaluated by entubulation of the rat sciatic nerve after a neurotmesis injury, with a 10 mm-gap between the nerve stumps. The results showed that the tube-guides were able to promote the regeneration of the nerve in a similar manner to what was observed with conventional techniques (nerve graft and end-to-end suture). Stereological analysis proved that nerve regeneration occurred, and both tube-guides presented fibre diameter and g-ratio closer to healthy sciatic nerves. The histomorphometric analysis of
Tibialis anterior
(TA) skeletal muscle showed decreased neurogenic atrophy in the porous tube-guides treated group, presenting measurements that are similar to the uninjured control.
Nerve tube-guides based on dextran and poly( -caprolactone) were successfully used in the recovery of a neurotmesis injury in rat model.</description><identifier>ISSN: 2047-4830</identifier><identifier>EISSN: 2047-4849</identifier><identifier>DOI: 10.1039/c9bm00901a</identifier><identifier>PMID: 31904045</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>Atrophy ; Data recovery ; Diameters ; In vivo methods and tests ; Injuries ; Muscles ; Nerves ; Performance assessment ; Regeneration ; Structural integrity</subject><ispartof>Biomaterials science, 2020-02, Vol.8 (3), p.798-811</ispartof><rights>Copyright Royal Society of Chemistry 2020</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c363t-13ef1b0b8fbfb830b2b5dfb79f6e9db631d92d38f69dd46adc7f3b5919158c73</citedby><cites>FETCH-LOGICAL-c363t-13ef1b0b8fbfb830b2b5dfb79f6e9db631d92d38f69dd46adc7f3b5919158c73</cites><orcidid>0000-0001-6330-4560 ; 0000-0002-3902-5374 ; 0000-0002-6962-831X ; 0000-0002-6871-9850 ; 0000-0002-4881-3313 ; 0000-0003-4496-6686 ; 0000-0003-1328-464X ; 0000-0003-2328-7174 ; 0000-0002-2055-8031 ; 0000-0002-7145-2472 ; 0000-0002-1143-2461 ; 0000-0001-9351-1704 ; 0000-0002-4795-7024 ; 0000-0002-0018-9363 ; 0000-0001-8664-2757 ; 0000-0003-1945-6377</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31904045$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pinho, Ana Catarina</creatorcontrib><creatorcontrib>Vieira Branquinho, Mariana</creatorcontrib><creatorcontrib>Alvites, Rui Damásio</creatorcontrib><creatorcontrib>Fonseca, Ana Clotilde</creatorcontrib><creatorcontrib>Caseiro, Ana Rita</creatorcontrib><creatorcontrib>Santos Pedrosa, Sílvia</creatorcontrib><creatorcontrib>Luís, Ana Lúcia</creatorcontrib><creatorcontrib>Pires, Isabel</creatorcontrib><creatorcontrib>Prada, Justina</creatorcontrib><creatorcontrib>Muratori, Luísa</creatorcontrib><creatorcontrib>Ronchi, Giulia</creatorcontrib><creatorcontrib>Geuna, Stefano</creatorcontrib><creatorcontrib>Santos, José Domingos</creatorcontrib><creatorcontrib>Maurício, Ana Colette</creatorcontrib><creatorcontrib>Serra, Arménio Coimbra</creatorcontrib><creatorcontrib>Coelho, Jorge Fernando Jordão</creatorcontrib><title>Dextran-based tube-guides for the regeneration of the rat sciatic nerve after neurotmesis injury</title><title>Biomaterials science</title><addtitle>Biomater Sci</addtitle><description>In this work, dextran-based nerve tube-guides were prepared, characterized and used in a standardized animal model of neurotmesis injury. Non-porous and porous transparent tube-guides were obtained by photocrosslinking of two co-macromonomers based on dextran and poly( -caprolactone) (PCL). Swelling capacity of the tube-guides ranged from 40-60% with no visible constriction of their inner diameter.
In vitro
hydrolytic degradation tests showed that the tube-guides maintained their structural integrity up to 6 months. The
in vivo
performance of the tube-guides was evaluated by entubulation of the rat sciatic nerve after a neurotmesis injury, with a 10 mm-gap between the nerve stumps. The results showed that the tube-guides were able to promote the regeneration of the nerve in a similar manner to what was observed with conventional techniques (nerve graft and end-to-end suture). Stereological analysis proved that nerve regeneration occurred, and both tube-guides presented fibre diameter and g-ratio closer to healthy sciatic nerves. The histomorphometric analysis of
Tibialis anterior
(TA) skeletal muscle showed decreased neurogenic atrophy in the porous tube-guides treated group, presenting measurements that are similar to the uninjured control.
Nerve tube-guides based on dextran and poly( -caprolactone) were successfully used in the recovery of a neurotmesis injury in rat model.</description><subject>Atrophy</subject><subject>Data recovery</subject><subject>Diameters</subject><subject>In vivo methods and tests</subject><subject>Injuries</subject><subject>Muscles</subject><subject>Nerves</subject><subject>Performance assessment</subject><subject>Regeneration</subject><subject>Structural integrity</subject><issn>2047-4830</issn><issn>2047-4849</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp90UtLAzEQAOAgipXai3cl4kWE1WSzrxxrfULFS-9rHpO6pbtbk6zYf290awUPhkCGmY9hmCB0RMklJYxfKS5rQjihYgcdxCTJo6RI-O42ZmSARs4tSDh5zklG99GAUU4SkqQH6OUGPrwVTSSFA419JyGad5UGh01rsX8FbGEODVjhq7bBrelzwmOnqpBTONTeAQvjwYa4s62vwVUOV82is-tDtGfE0sFo8w7R7O52NnmIps_3j5PxNFIsYz6iDAyVRBZGGhmGlrFMtZE5NxlwLTNGNY81K0zGtU4yoVVumEw55TQtVM6G6Lxvu7LtWwfOl3XlFCyXooG2c2XMGOMs5pQGevaHLtrONmG4oFISh1sUQV30StnWOQumXNmqFnZdUlJ-bb6c8Oun782PAz7ZtOxkDXpLf_YcwGkPrFPb6u_XlSttgjn-z7BP-KOTvg</recordid><startdate>20200207</startdate><enddate>20200207</enddate><creator>Pinho, Ana Catarina</creator><creator>Vieira Branquinho, Mariana</creator><creator>Alvites, Rui Damásio</creator><creator>Fonseca, Ana Clotilde</creator><creator>Caseiro, Ana Rita</creator><creator>Santos Pedrosa, Sílvia</creator><creator>Luís, Ana Lúcia</creator><creator>Pires, Isabel</creator><creator>Prada, Justina</creator><creator>Muratori, Luísa</creator><creator>Ronchi, Giulia</creator><creator>Geuna, Stefano</creator><creator>Santos, José Domingos</creator><creator>Maurício, Ana Colette</creator><creator>Serra, Arménio Coimbra</creator><creator>Coelho, Jorge Fernando Jordão</creator><general>Royal Society of Chemistry</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-6330-4560</orcidid><orcidid>https://orcid.org/0000-0002-3902-5374</orcidid><orcidid>https://orcid.org/0000-0002-6962-831X</orcidid><orcidid>https://orcid.org/0000-0002-6871-9850</orcidid><orcidid>https://orcid.org/0000-0002-4881-3313</orcidid><orcidid>https://orcid.org/0000-0003-4496-6686</orcidid><orcidid>https://orcid.org/0000-0003-1328-464X</orcidid><orcidid>https://orcid.org/0000-0003-2328-7174</orcidid><orcidid>https://orcid.org/0000-0002-2055-8031</orcidid><orcidid>https://orcid.org/0000-0002-7145-2472</orcidid><orcidid>https://orcid.org/0000-0002-1143-2461</orcidid><orcidid>https://orcid.org/0000-0001-9351-1704</orcidid><orcidid>https://orcid.org/0000-0002-4795-7024</orcidid><orcidid>https://orcid.org/0000-0002-0018-9363</orcidid><orcidid>https://orcid.org/0000-0001-8664-2757</orcidid><orcidid>https://orcid.org/0000-0003-1945-6377</orcidid></search><sort><creationdate>20200207</creationdate><title>Dextran-based tube-guides for the regeneration of the rat sciatic nerve after neurotmesis injury</title><author>Pinho, Ana Catarina ; Vieira Branquinho, Mariana ; Alvites, Rui Damásio ; Fonseca, Ana Clotilde ; Caseiro, Ana Rita ; Santos Pedrosa, Sílvia ; Luís, Ana Lúcia ; Pires, Isabel ; Prada, Justina ; Muratori, Luísa ; Ronchi, Giulia ; Geuna, Stefano ; Santos, José Domingos ; Maurício, Ana Colette ; Serra, Arménio Coimbra ; Coelho, Jorge Fernando Jordão</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c363t-13ef1b0b8fbfb830b2b5dfb79f6e9db631d92d38f69dd46adc7f3b5919158c73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Atrophy</topic><topic>Data recovery</topic><topic>Diameters</topic><topic>In vivo methods and tests</topic><topic>Injuries</topic><topic>Muscles</topic><topic>Nerves</topic><topic>Performance assessment</topic><topic>Regeneration</topic><topic>Structural integrity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pinho, Ana Catarina</creatorcontrib><creatorcontrib>Vieira Branquinho, Mariana</creatorcontrib><creatorcontrib>Alvites, Rui Damásio</creatorcontrib><creatorcontrib>Fonseca, Ana Clotilde</creatorcontrib><creatorcontrib>Caseiro, Ana Rita</creatorcontrib><creatorcontrib>Santos Pedrosa, Sílvia</creatorcontrib><creatorcontrib>Luís, Ana Lúcia</creatorcontrib><creatorcontrib>Pires, Isabel</creatorcontrib><creatorcontrib>Prada, Justina</creatorcontrib><creatorcontrib>Muratori, Luísa</creatorcontrib><creatorcontrib>Ronchi, Giulia</creatorcontrib><creatorcontrib>Geuna, Stefano</creatorcontrib><creatorcontrib>Santos, José Domingos</creatorcontrib><creatorcontrib>Maurício, Ana Colette</creatorcontrib><creatorcontrib>Serra, Arménio Coimbra</creatorcontrib><creatorcontrib>Coelho, Jorge Fernando Jordão</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>MEDLINE - Academic</collection><jtitle>Biomaterials science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pinho, Ana Catarina</au><au>Vieira Branquinho, Mariana</au><au>Alvites, Rui Damásio</au><au>Fonseca, Ana Clotilde</au><au>Caseiro, Ana Rita</au><au>Santos Pedrosa, Sílvia</au><au>Luís, Ana Lúcia</au><au>Pires, Isabel</au><au>Prada, Justina</au><au>Muratori, Luísa</au><au>Ronchi, Giulia</au><au>Geuna, Stefano</au><au>Santos, José Domingos</au><au>Maurício, Ana Colette</au><au>Serra, Arménio Coimbra</au><au>Coelho, Jorge Fernando Jordão</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dextran-based tube-guides for the regeneration of the rat sciatic nerve after neurotmesis injury</atitle><jtitle>Biomaterials science</jtitle><addtitle>Biomater Sci</addtitle><date>2020-02-07</date><risdate>2020</risdate><volume>8</volume><issue>3</issue><spage>798</spage><epage>811</epage><pages>798-811</pages><issn>2047-4830</issn><eissn>2047-4849</eissn><abstract>In this work, dextran-based nerve tube-guides were prepared, characterized and used in a standardized animal model of neurotmesis injury. Non-porous and porous transparent tube-guides were obtained by photocrosslinking of two co-macromonomers based on dextran and poly( -caprolactone) (PCL). Swelling capacity of the tube-guides ranged from 40-60% with no visible constriction of their inner diameter.
In vitro
hydrolytic degradation tests showed that the tube-guides maintained their structural integrity up to 6 months. The
in vivo
performance of the tube-guides was evaluated by entubulation of the rat sciatic nerve after a neurotmesis injury, with a 10 mm-gap between the nerve stumps. The results showed that the tube-guides were able to promote the regeneration of the nerve in a similar manner to what was observed with conventional techniques (nerve graft and end-to-end suture). Stereological analysis proved that nerve regeneration occurred, and both tube-guides presented fibre diameter and g-ratio closer to healthy sciatic nerves. The histomorphometric analysis of
Tibialis anterior
(TA) skeletal muscle showed decreased neurogenic atrophy in the porous tube-guides treated group, presenting measurements that are similar to the uninjured control.
Nerve tube-guides based on dextran and poly( -caprolactone) were successfully used in the recovery of a neurotmesis injury in rat model.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>31904045</pmid><doi>10.1039/c9bm00901a</doi><tpages>14</tpages><orcidid>https://orcid.org/0000-0001-6330-4560</orcidid><orcidid>https://orcid.org/0000-0002-3902-5374</orcidid><orcidid>https://orcid.org/0000-0002-6962-831X</orcidid><orcidid>https://orcid.org/0000-0002-6871-9850</orcidid><orcidid>https://orcid.org/0000-0002-4881-3313</orcidid><orcidid>https://orcid.org/0000-0003-4496-6686</orcidid><orcidid>https://orcid.org/0000-0003-1328-464X</orcidid><orcidid>https://orcid.org/0000-0003-2328-7174</orcidid><orcidid>https://orcid.org/0000-0002-2055-8031</orcidid><orcidid>https://orcid.org/0000-0002-7145-2472</orcidid><orcidid>https://orcid.org/0000-0002-1143-2461</orcidid><orcidid>https://orcid.org/0000-0001-9351-1704</orcidid><orcidid>https://orcid.org/0000-0002-4795-7024</orcidid><orcidid>https://orcid.org/0000-0002-0018-9363</orcidid><orcidid>https://orcid.org/0000-0001-8664-2757</orcidid><orcidid>https://orcid.org/0000-0003-1945-6377</orcidid></addata></record> |
| fulltext | fulltext |
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| ispartof | Biomaterials science, 2020-02, Vol.8 (3), p.798-811 |
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| source | Royal Society Of Chemistry Journals |
| subjects | Atrophy Data recovery Diameters In vivo methods and tests Injuries Muscles Nerves Performance assessment Regeneration Structural integrity |
| title | Dextran-based tube-guides for the regeneration of the rat sciatic nerve after neurotmesis injury |
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