Toxicity of five Cry proteins against the insect pest Acanthoscelides obtectus (Coleoptera: Chrisomelidae: Bruchinae)
[Display omitted] •Five Bt Cry proteins were applied to beans to control Acanthoscelides obtectus.•Cry1Ba, Cry1Ia, Cry3Aa, Cry7Ab, and Cry23/37 killed adult A. obtectus.•Adult emergence was reduced in larvae fed Cry protein-treated beans.•Damage to beans by A. obtectus was reduced in Cry protein-tre...
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| Veröffentlicht in: | Journal of invertebrate pathology 2020-01, Vol.169, p.107295, Article 107295 |
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| creator | Rodríguez-González, Álvaro Porteous-Álvarez, Alejandra J. Val, Mario Del Casquero, Pedro A. Escriche, Baltasar |
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•Five Bt Cry proteins were applied to beans to control Acanthoscelides obtectus.•Cry1Ba, Cry1Ia, Cry3Aa, Cry7Ab, and Cry23/37 killed adult A. obtectus.•Adult emergence was reduced in larvae fed Cry protein-treated beans.•Damage to beans by A. obtectus was reduced in Cry protein-treated beans.
The beetle Acanthoscelides obtectus (Say) causes severe post-harvest losses in the common bean (Phaseolus vulgaris). Under laboratory conditions, the susceptibility of A. obtectus to five coleopteran-specific Cry toxic proteins from Bacillus thuringiensis (Cry1Ba, Cry1Ia, Cry3Aa, Cry7Ab, and Cry23/37) was evaluated. After 30 days exposure, Cry proteins demonstrated high activity against A. obtectus adults (100% mortality). Proteins showed statistical differences in toxicity parameters compared to the control treatment, but the parameters were similar among them, and indicated that the final toxic effects can be observed after the 24th day. The toxic effects on A. obtectus larvae were evaluated indirectly by allowing adults to oviposit on treated beans and recording the emergence of F1 adults. All treatments resulted in a lower rate of successful emergence compared to the control treatment, ranging from 60% (Cry23/37) to 10% (Cry1Ia) reduction in eclosion. Finally, to evaluate the ability of Cry proteins to protect the beans against A. obtectus; the number of beans infested, the number of holes in each bean and bean weight loss were determined 45 days after the treatment. The parameters showed significant bean protection by all Cry proteins analyzed compared to control treatment. Cry23/37 showed the best results, however, results for the other proteins were similar. The proteins belong to different Cry protein families, which suggest that they could be used in combination to increase plant protection without compromising resistance management. Moreover, adult emergence and bean protection results indicate differences among the proteins, which may suggest different modes of action. Our results indicate that the studied Cry proteins can be applied for the control of A. obtectus larvae and adults. |
| doi_str_mv | 10.1016/j.jip.2019.107295 |
| format | Article |
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•Five Bt Cry proteins were applied to beans to control Acanthoscelides obtectus.•Cry1Ba, Cry1Ia, Cry3Aa, Cry7Ab, and Cry23/37 killed adult A. obtectus.•Adult emergence was reduced in larvae fed Cry protein-treated beans.•Damage to beans by A. obtectus was reduced in Cry protein-treated beans.
The beetle Acanthoscelides obtectus (Say) causes severe post-harvest losses in the common bean (Phaseolus vulgaris). Under laboratory conditions, the susceptibility of A. obtectus to five coleopteran-specific Cry toxic proteins from Bacillus thuringiensis (Cry1Ba, Cry1Ia, Cry3Aa, Cry7Ab, and Cry23/37) was evaluated. After 30 days exposure, Cry proteins demonstrated high activity against A. obtectus adults (100% mortality). Proteins showed statistical differences in toxicity parameters compared to the control treatment, but the parameters were similar among them, and indicated that the final toxic effects can be observed after the 24th day. The toxic effects on A. obtectus larvae were evaluated indirectly by allowing adults to oviposit on treated beans and recording the emergence of F1 adults. All treatments resulted in a lower rate of successful emergence compared to the control treatment, ranging from 60% (Cry23/37) to 10% (Cry1Ia) reduction in eclosion. Finally, to evaluate the ability of Cry proteins to protect the beans against A. obtectus; the number of beans infested, the number of holes in each bean and bean weight loss were determined 45 days after the treatment. The parameters showed significant bean protection by all Cry proteins analyzed compared to control treatment. Cry23/37 showed the best results, however, results for the other proteins were similar. The proteins belong to different Cry protein families, which suggest that they could be used in combination to increase plant protection without compromising resistance management. Moreover, adult emergence and bean protection results indicate differences among the proteins, which may suggest different modes of action. Our results indicate that the studied Cry proteins can be applied for the control of A. obtectus larvae and adults.</description><identifier>ISSN: 0022-2011</identifier><identifier>EISSN: 1096-0805</identifier><identifier>DOI: 10.1016/j.jip.2019.107295</identifier><identifier>PMID: 31783031</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Bacillus thuringiensis - chemistry ; Bacillus thuringiensis Toxins ; Bacterial Proteins - pharmacology ; Bacterium ; Bean weevil ; Biological Control Agents ; Coleoptera - growth & development ; Common beans ; Cristal proteins ; Endotoxins ; Hemolysin Proteins ; Insect Control ; Larva ; Pest Control, Biological</subject><ispartof>Journal of invertebrate pathology, 2020-01, Vol.169, p.107295, Article 107295</ispartof><rights>2019 Elsevier Inc.</rights><rights>Copyright © 2019 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c353t-b7ff0c5e9d58ce5016538ec0581e3064942dfcd997e05ab3234b448f811a71453</citedby><cites>FETCH-LOGICAL-c353t-b7ff0c5e9d58ce5016538ec0581e3064942dfcd997e05ab3234b448f811a71453</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jip.2019.107295$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31783031$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rodríguez-González, Álvaro</creatorcontrib><creatorcontrib>Porteous-Álvarez, Alejandra J.</creatorcontrib><creatorcontrib>Val, Mario Del</creatorcontrib><creatorcontrib>Casquero, Pedro A.</creatorcontrib><creatorcontrib>Escriche, Baltasar</creatorcontrib><title>Toxicity of five Cry proteins against the insect pest Acanthoscelides obtectus (Coleoptera: Chrisomelidae: Bruchinae)</title><title>Journal of invertebrate pathology</title><addtitle>J Invertebr Pathol</addtitle><description>[Display omitted]
•Five Bt Cry proteins were applied to beans to control Acanthoscelides obtectus.•Cry1Ba, Cry1Ia, Cry3Aa, Cry7Ab, and Cry23/37 killed adult A. obtectus.•Adult emergence was reduced in larvae fed Cry protein-treated beans.•Damage to beans by A. obtectus was reduced in Cry protein-treated beans.
The beetle Acanthoscelides obtectus (Say) causes severe post-harvest losses in the common bean (Phaseolus vulgaris). Under laboratory conditions, the susceptibility of A. obtectus to five coleopteran-specific Cry toxic proteins from Bacillus thuringiensis (Cry1Ba, Cry1Ia, Cry3Aa, Cry7Ab, and Cry23/37) was evaluated. After 30 days exposure, Cry proteins demonstrated high activity against A. obtectus adults (100% mortality). Proteins showed statistical differences in toxicity parameters compared to the control treatment, but the parameters were similar among them, and indicated that the final toxic effects can be observed after the 24th day. The toxic effects on A. obtectus larvae were evaluated indirectly by allowing adults to oviposit on treated beans and recording the emergence of F1 adults. All treatments resulted in a lower rate of successful emergence compared to the control treatment, ranging from 60% (Cry23/37) to 10% (Cry1Ia) reduction in eclosion. Finally, to evaluate the ability of Cry proteins to protect the beans against A. obtectus; the number of beans infested, the number of holes in each bean and bean weight loss were determined 45 days after the treatment. The parameters showed significant bean protection by all Cry proteins analyzed compared to control treatment. Cry23/37 showed the best results, however, results for the other proteins were similar. The proteins belong to different Cry protein families, which suggest that they could be used in combination to increase plant protection without compromising resistance management. Moreover, adult emergence and bean protection results indicate differences among the proteins, which may suggest different modes of action. Our results indicate that the studied Cry proteins can be applied for the control of A. obtectus larvae and adults.</description><subject>Animals</subject><subject>Bacillus thuringiensis - chemistry</subject><subject>Bacillus thuringiensis Toxins</subject><subject>Bacterial Proteins - pharmacology</subject><subject>Bacterium</subject><subject>Bean weevil</subject><subject>Biological Control Agents</subject><subject>Coleoptera - growth & development</subject><subject>Common beans</subject><subject>Cristal proteins</subject><subject>Endotoxins</subject><subject>Hemolysin Proteins</subject><subject>Insect Control</subject><subject>Larva</subject><subject>Pest Control, Biological</subject><issn>0022-2011</issn><issn>1096-0805</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtOwzAQRS0EoqXwAWyQl7BIseM4j7IqES-pEpuythxnQhy1cWS7Ff17XAVYspo7mntHMweha0rmlND0vpt3epjHhBahz-KCn6ApJUUakZzwUzQlJI6jMKYTdOFcR4LiaXGOJoxmOSOMTtFubb600v6ATYMbvQdc2gMerPGge4flpwzFY98CDgKUxwOEfqlk71vjFGx0DQ6byofZzuHb0mzADB6sXOCytdqZ7dEjYYEf7U61updwd4nOGrlxcPVTZ-jj-Wldvkar95e3crmKFOPMR1XWNERxKGqeK-DhZc5yUITnFBhJkyKJ60bVRZEB4bJiMUuqJMmbnFKZ0YSzGaLjXmWNcxYaMVi9lfYgKBFHhKITAaE4IhQjwpC5GTPDrtpC_Zf4ZRYMD6MBwuV7DVY4paFXUGsbIIja6H_WfwMec4Kj</recordid><startdate>202001</startdate><enddate>202001</enddate><creator>Rodríguez-González, Álvaro</creator><creator>Porteous-Álvarez, Alejandra J.</creator><creator>Val, Mario Del</creator><creator>Casquero, Pedro A.</creator><creator>Escriche, Baltasar</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>202001</creationdate><title>Toxicity of five Cry proteins against the insect pest Acanthoscelides obtectus (Coleoptera: Chrisomelidae: Bruchinae)</title><author>Rodríguez-González, Álvaro ; Porteous-Álvarez, Alejandra J. ; Val, Mario Del ; Casquero, Pedro A. ; Escriche, Baltasar</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c353t-b7ff0c5e9d58ce5016538ec0581e3064942dfcd997e05ab3234b448f811a71453</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Animals</topic><topic>Bacillus thuringiensis - chemistry</topic><topic>Bacillus thuringiensis Toxins</topic><topic>Bacterial Proteins - pharmacology</topic><topic>Bacterium</topic><topic>Bean weevil</topic><topic>Biological Control Agents</topic><topic>Coleoptera - growth & development</topic><topic>Common beans</topic><topic>Cristal proteins</topic><topic>Endotoxins</topic><topic>Hemolysin Proteins</topic><topic>Insect Control</topic><topic>Larva</topic><topic>Pest Control, Biological</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rodríguez-González, Álvaro</creatorcontrib><creatorcontrib>Porteous-Álvarez, Alejandra J.</creatorcontrib><creatorcontrib>Val, Mario Del</creatorcontrib><creatorcontrib>Casquero, Pedro A.</creatorcontrib><creatorcontrib>Escriche, Baltasar</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Journal of invertebrate pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rodríguez-González, Álvaro</au><au>Porteous-Álvarez, Alejandra J.</au><au>Val, Mario Del</au><au>Casquero, Pedro A.</au><au>Escriche, Baltasar</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Toxicity of five Cry proteins against the insect pest Acanthoscelides obtectus (Coleoptera: Chrisomelidae: Bruchinae)</atitle><jtitle>Journal of invertebrate pathology</jtitle><addtitle>J Invertebr Pathol</addtitle><date>2020-01</date><risdate>2020</risdate><volume>169</volume><spage>107295</spage><pages>107295-</pages><artnum>107295</artnum><issn>0022-2011</issn><eissn>1096-0805</eissn><abstract>[Display omitted]
•Five Bt Cry proteins were applied to beans to control Acanthoscelides obtectus.•Cry1Ba, Cry1Ia, Cry3Aa, Cry7Ab, and Cry23/37 killed adult A. obtectus.•Adult emergence was reduced in larvae fed Cry protein-treated beans.•Damage to beans by A. obtectus was reduced in Cry protein-treated beans.
The beetle Acanthoscelides obtectus (Say) causes severe post-harvest losses in the common bean (Phaseolus vulgaris). Under laboratory conditions, the susceptibility of A. obtectus to five coleopteran-specific Cry toxic proteins from Bacillus thuringiensis (Cry1Ba, Cry1Ia, Cry3Aa, Cry7Ab, and Cry23/37) was evaluated. After 30 days exposure, Cry proteins demonstrated high activity against A. obtectus adults (100% mortality). Proteins showed statistical differences in toxicity parameters compared to the control treatment, but the parameters were similar among them, and indicated that the final toxic effects can be observed after the 24th day. The toxic effects on A. obtectus larvae were evaluated indirectly by allowing adults to oviposit on treated beans and recording the emergence of F1 adults. All treatments resulted in a lower rate of successful emergence compared to the control treatment, ranging from 60% (Cry23/37) to 10% (Cry1Ia) reduction in eclosion. Finally, to evaluate the ability of Cry proteins to protect the beans against A. obtectus; the number of beans infested, the number of holes in each bean and bean weight loss were determined 45 days after the treatment. The parameters showed significant bean protection by all Cry proteins analyzed compared to control treatment. Cry23/37 showed the best results, however, results for the other proteins were similar. The proteins belong to different Cry protein families, which suggest that they could be used in combination to increase plant protection without compromising resistance management. Moreover, adult emergence and bean protection results indicate differences among the proteins, which may suggest different modes of action. Our results indicate that the studied Cry proteins can be applied for the control of A. obtectus larvae and adults.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>31783031</pmid><doi>10.1016/j.jip.2019.107295</doi></addata></record> |
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| subjects | Animals Bacillus thuringiensis - chemistry Bacillus thuringiensis Toxins Bacterial Proteins - pharmacology Bacterium Bean weevil Biological Control Agents Coleoptera - growth & development Common beans Cristal proteins Endotoxins Hemolysin Proteins Insect Control Larva Pest Control, Biological |
| title | Toxicity of five Cry proteins against the insect pest Acanthoscelides obtectus (Coleoptera: Chrisomelidae: Bruchinae) |
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