Characterization of immune response against Mycobacterium marinum infection in the main hematopoietic organ of adult zebrafish (Danio rerio)
Tuberculosis remains a major global health challenge. To gain information about genes important for defense against tuberculosis, we used a well-established tuberculosis model; Mycobacterium marinum infection in adult zebrafish. To characterize the immunological response to mycobacterial infection a...
Gespeichert in:
| Veröffentlicht in: | Developmental and comparative immunology 2020-02, Vol.103, p.103523-103523, Article 103523 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 103523 |
|---|---|
| container_issue | |
| container_start_page | 103523 |
| container_title | Developmental and comparative immunology |
| container_volume | 103 |
| creator | Harjula, Sanna-Kaisa E. Saralahti, Anni K. Ojanen, Markus J.T. Rantapero, Tommi Uusi-Mäkelä, Meri I.E. Nykter, Matti Lohi, Olli Parikka, Mataleena Rämet, Mika |
| description | Tuberculosis remains a major global health challenge. To gain information about genes important for defense against tuberculosis, we used a well-established tuberculosis model; Mycobacterium marinum infection in adult zebrafish. To characterize the immunological response to mycobacterial infection at 14 days post infection, we performed a whole-genome level transcriptome analysis using cells from kidney, the main hematopoietic organ of adult zebrafish. Among the upregulated genes, those associated with immune signaling and regulation formed the largest category, whereas the largest group of downregulated genes had a metabolic role. We also performed a forward genetic screen in adult zebrafish and identified a fish line with severely impaired survival during chronic mycobacterial infection. Based on transcriptome analysis, these fish have decreased expression of several immunological genes. Taken together, these results give new information about the genes involved in the defense against mycobacterial infection in zebrafish.
•201 genes were up- or downregulated at 14 days post zebrafish M. marinum infection.•Among upregulated genes, innate immune processes were most enriched.•Among downregulated genes, metabolic processes were most enriched.•We identified a mutant line with impaired resistance against M. marinum infection.•The mutants display deficiency in T cell activation and inflammasome function. |
| doi_str_mv | 10.1016/j.dci.2019.103523 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmed_primary_31626817</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0145305X19304434</els_id><sourcerecordid>2307127699</sourcerecordid><originalsourceid>FETCH-LOGICAL-c424t-1de866b2d9121bb28c90c6fe0405ff1f3bff2c71ee89bef333676691fee14a8f3</originalsourceid><addsrcrecordid>eNqNkctu1DAUhi0EotPCA7BBlti0Qhl8SRxHXaFwlYrYgMTOcpzjjkcTe7AdUPsMPDTuZOiCBSKSY1v-_uPLh9AzStaUUPFqux6NWzNCuzLnDeMP0IrKtqsIkd1DtCK0bipOmm8n6DSlLSmfpOQxOuFUMCFpu0K_-o2O2mSI7lZnFzwOFrtpmj3gCGkffAKsr7XzKeNPNyYMCzxPeNLR-dI7b8Ecos7jvIGyUAYbmHQO--AgO4NDvNaH0nqcdxnfwhC1dWmDz99o70LZKrpw8QQ9snqX4OmxP0Nf37390n-orj6__9i_vqpMzepc0RGkEAMbO8roMDBpOmKEBVKTxlpq-WAtMy0FkN0AlnMuWiE6agForaXlZ-h8qbuP4fsMKavJJQO7nfYQ5qQYJy1lrei6gr74C92GOfpyukLVdcOaTrJC0YUyMaQUwap9dOV9bhQl6k6V2qqiSt2pUouqknl-rDwPE4z3iT9uCvByAX7CEGwyDryBe6y4bA6tLX9GCi3_n-5dPsjuw-xziV4uUShv_sNBVMf46GIxq8bg_nGP35x5xs8</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2344525982</pqid></control><display><type>article</type><title>Characterization of immune response against Mycobacterium marinum infection in the main hematopoietic organ of adult zebrafish (Danio rerio)</title><source>Web of Science - Science Citation Index Expanded - 2020<img src="https://exlibris-pub.s3.amazonaws.com/fromwos-v2.jpg" /></source><source>Access via ScienceDirect (Elsevier)</source><creator>Harjula, Sanna-Kaisa E. ; Saralahti, Anni K. ; Ojanen, Markus J.T. ; Rantapero, Tommi ; Uusi-Mäkelä, Meri I.E. ; Nykter, Matti ; Lohi, Olli ; Parikka, Mataleena ; Rämet, Mika</creator><creatorcontrib>Harjula, Sanna-Kaisa E. ; Saralahti, Anni K. ; Ojanen, Markus J.T. ; Rantapero, Tommi ; Uusi-Mäkelä, Meri I.E. ; Nykter, Matti ; Lohi, Olli ; Parikka, Mataleena ; Rämet, Mika</creatorcontrib><description>Tuberculosis remains a major global health challenge. To gain information about genes important for defense against tuberculosis, we used a well-established tuberculosis model; Mycobacterium marinum infection in adult zebrafish. To characterize the immunological response to mycobacterial infection at 14 days post infection, we performed a whole-genome level transcriptome analysis using cells from kidney, the main hematopoietic organ of adult zebrafish. Among the upregulated genes, those associated with immune signaling and regulation formed the largest category, whereas the largest group of downregulated genes had a metabolic role. We also performed a forward genetic screen in adult zebrafish and identified a fish line with severely impaired survival during chronic mycobacterial infection. Based on transcriptome analysis, these fish have decreased expression of several immunological genes. Taken together, these results give new information about the genes involved in the defense against mycobacterial infection in zebrafish.
•201 genes were up- or downregulated at 14 days post zebrafish M. marinum infection.•Among upregulated genes, innate immune processes were most enriched.•Among downregulated genes, metabolic processes were most enriched.•We identified a mutant line with impaired resistance against M. marinum infection.•The mutants display deficiency in T cell activation and inflammasome function.</description><identifier>ISSN: 0145-305X</identifier><identifier>EISSN: 1879-0089</identifier><identifier>DOI: 10.1016/j.dci.2019.103523</identifier><identifier>PMID: 31626817</identifier><language>eng</language><publisher>OXFORD: Elsevier Ltd</publisher><subject>Chronic infection ; Danio rerio ; Fisheries ; Forward genetic screen ; Gene expression ; Genes ; Genetic screening ; Genomes ; Global health ; Immune response ; Immune system ; Immunology ; Infections ; Life Sciences & Biomedicine ; Mycobacterium marinum ; Science & Technology ; Transcriptome analysis ; Tuberculosis ; Veterinary Sciences ; Zebrafish ; Zoology</subject><ispartof>Developmental and comparative immunology, 2020-02, Vol.103, p.103523-103523, Article 103523</ispartof><rights>2019 The Authors</rights><rights>Copyright © 2019 The Authors. Published by Elsevier Ltd.. All rights reserved.</rights><rights>Copyright Elsevier Science Ltd. Feb 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>true</woscitedreferencessubscribed><woscitedreferencescount>11</woscitedreferencescount><woscitedreferencesoriginalsourcerecordid>wos000500050700020</woscitedreferencesoriginalsourcerecordid><citedby>FETCH-LOGICAL-c424t-1de866b2d9121bb28c90c6fe0405ff1f3bff2c71ee89bef333676691fee14a8f3</citedby><cites>FETCH-LOGICAL-c424t-1de866b2d9121bb28c90c6fe0405ff1f3bff2c71ee89bef333676691fee14a8f3</cites><orcidid>0000-0001-6623-844X ; 0000-0001-6956-2843 ; 0000-0003-3750-2779 ; 0000-0002-6145-6875 ; 0000-0001-9195-0797 ; 0000-0001-5555-3815</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.dci.2019.103523$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,28253,46000</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31626817$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Harjula, Sanna-Kaisa E.</creatorcontrib><creatorcontrib>Saralahti, Anni K.</creatorcontrib><creatorcontrib>Ojanen, Markus J.T.</creatorcontrib><creatorcontrib>Rantapero, Tommi</creatorcontrib><creatorcontrib>Uusi-Mäkelä, Meri I.E.</creatorcontrib><creatorcontrib>Nykter, Matti</creatorcontrib><creatorcontrib>Lohi, Olli</creatorcontrib><creatorcontrib>Parikka, Mataleena</creatorcontrib><creatorcontrib>Rämet, Mika</creatorcontrib><title>Characterization of immune response against Mycobacterium marinum infection in the main hematopoietic organ of adult zebrafish (Danio rerio)</title><title>Developmental and comparative immunology</title><addtitle>DEV COMP IMMUNOL</addtitle><addtitle>Dev Comp Immunol</addtitle><description>Tuberculosis remains a major global health challenge. To gain information about genes important for defense against tuberculosis, we used a well-established tuberculosis model; Mycobacterium marinum infection in adult zebrafish. To characterize the immunological response to mycobacterial infection at 14 days post infection, we performed a whole-genome level transcriptome analysis using cells from kidney, the main hematopoietic organ of adult zebrafish. Among the upregulated genes, those associated with immune signaling and regulation formed the largest category, whereas the largest group of downregulated genes had a metabolic role. We also performed a forward genetic screen in adult zebrafish and identified a fish line with severely impaired survival during chronic mycobacterial infection. Based on transcriptome analysis, these fish have decreased expression of several immunological genes. Taken together, these results give new information about the genes involved in the defense against mycobacterial infection in zebrafish.
•201 genes were up- or downregulated at 14 days post zebrafish M. marinum infection.•Among upregulated genes, innate immune processes were most enriched.•Among downregulated genes, metabolic processes were most enriched.•We identified a mutant line with impaired resistance against M. marinum infection.•The mutants display deficiency in T cell activation and inflammasome function.</description><subject>Chronic infection</subject><subject>Danio rerio</subject><subject>Fisheries</subject><subject>Forward genetic screen</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Genetic screening</subject><subject>Genomes</subject><subject>Global health</subject><subject>Immune response</subject><subject>Immune system</subject><subject>Immunology</subject><subject>Infections</subject><subject>Life Sciences & Biomedicine</subject><subject>Mycobacterium marinum</subject><subject>Science & Technology</subject><subject>Transcriptome analysis</subject><subject>Tuberculosis</subject><subject>Veterinary Sciences</subject><subject>Zebrafish</subject><subject>Zoology</subject><issn>0145-305X</issn><issn>1879-0089</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>AOWDO</sourceid><recordid>eNqNkctu1DAUhi0EotPCA7BBlti0Qhl8SRxHXaFwlYrYgMTOcpzjjkcTe7AdUPsMPDTuZOiCBSKSY1v-_uPLh9AzStaUUPFqux6NWzNCuzLnDeMP0IrKtqsIkd1DtCK0bipOmm8n6DSlLSmfpOQxOuFUMCFpu0K_-o2O2mSI7lZnFzwOFrtpmj3gCGkffAKsr7XzKeNPNyYMCzxPeNLR-dI7b8Ecos7jvIGyUAYbmHQO--AgO4NDvNaH0nqcdxnfwhC1dWmDz99o70LZKrpw8QQ9snqX4OmxP0Nf37390n-orj6__9i_vqpMzepc0RGkEAMbO8roMDBpOmKEBVKTxlpq-WAtMy0FkN0AlnMuWiE6agForaXlZ-h8qbuP4fsMKavJJQO7nfYQ5qQYJy1lrei6gr74C92GOfpyukLVdcOaTrJC0YUyMaQUwap9dOV9bhQl6k6V2qqiSt2pUouqknl-rDwPE4z3iT9uCvByAX7CEGwyDryBe6y4bA6tLX9GCi3_n-5dPsjuw-xziV4uUShv_sNBVMf46GIxq8bg_nGP35x5xs8</recordid><startdate>202002</startdate><enddate>202002</enddate><creator>Harjula, Sanna-Kaisa E.</creator><creator>Saralahti, Anni K.</creator><creator>Ojanen, Markus J.T.</creator><creator>Rantapero, Tommi</creator><creator>Uusi-Mäkelä, Meri I.E.</creator><creator>Nykter, Matti</creator><creator>Lohi, Olli</creator><creator>Parikka, Mataleena</creator><creator>Rämet, Mika</creator><general>Elsevier Ltd</general><general>Elsevier</general><general>Elsevier Science Ltd</general><scope>6I.</scope><scope>AAFTH</scope><scope>AOWDO</scope><scope>BLEPL</scope><scope>DTL</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T5</scope><scope>C1K</scope><scope>H94</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-6623-844X</orcidid><orcidid>https://orcid.org/0000-0001-6956-2843</orcidid><orcidid>https://orcid.org/0000-0003-3750-2779</orcidid><orcidid>https://orcid.org/0000-0002-6145-6875</orcidid><orcidid>https://orcid.org/0000-0001-9195-0797</orcidid><orcidid>https://orcid.org/0000-0001-5555-3815</orcidid></search><sort><creationdate>202002</creationdate><title>Characterization of immune response against Mycobacterium marinum infection in the main hematopoietic organ of adult zebrafish (Danio rerio)</title><author>Harjula, Sanna-Kaisa E. ; Saralahti, Anni K. ; Ojanen, Markus J.T. ; Rantapero, Tommi ; Uusi-Mäkelä, Meri I.E. ; Nykter, Matti ; Lohi, Olli ; Parikka, Mataleena ; Rämet, Mika</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c424t-1de866b2d9121bb28c90c6fe0405ff1f3bff2c71ee89bef333676691fee14a8f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Chronic infection</topic><topic>Danio rerio</topic><topic>Fisheries</topic><topic>Forward genetic screen</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Genetic screening</topic><topic>Genomes</topic><topic>Global health</topic><topic>Immune response</topic><topic>Immune system</topic><topic>Immunology</topic><topic>Infections</topic><topic>Life Sciences & Biomedicine</topic><topic>Mycobacterium marinum</topic><topic>Science & Technology</topic><topic>Transcriptome analysis</topic><topic>Tuberculosis</topic><topic>Veterinary Sciences</topic><topic>Zebrafish</topic><topic>Zoology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Harjula, Sanna-Kaisa E.</creatorcontrib><creatorcontrib>Saralahti, Anni K.</creatorcontrib><creatorcontrib>Ojanen, Markus J.T.</creatorcontrib><creatorcontrib>Rantapero, Tommi</creatorcontrib><creatorcontrib>Uusi-Mäkelä, Meri I.E.</creatorcontrib><creatorcontrib>Nykter, Matti</creatorcontrib><creatorcontrib>Lohi, Olli</creatorcontrib><creatorcontrib>Parikka, Mataleena</creatorcontrib><creatorcontrib>Rämet, Mika</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Web of Science - Science Citation Index Expanded - 2020</collection><collection>Web of Science Core Collection</collection><collection>Science Citation Index Expanded</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Developmental and comparative immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Harjula, Sanna-Kaisa E.</au><au>Saralahti, Anni K.</au><au>Ojanen, Markus J.T.</au><au>Rantapero, Tommi</au><au>Uusi-Mäkelä, Meri I.E.</au><au>Nykter, Matti</au><au>Lohi, Olli</au><au>Parikka, Mataleena</au><au>Rämet, Mika</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of immune response against Mycobacterium marinum infection in the main hematopoietic organ of adult zebrafish (Danio rerio)</atitle><jtitle>Developmental and comparative immunology</jtitle><stitle>DEV COMP IMMUNOL</stitle><addtitle>Dev Comp Immunol</addtitle><date>2020-02</date><risdate>2020</risdate><volume>103</volume><spage>103523</spage><epage>103523</epage><pages>103523-103523</pages><artnum>103523</artnum><issn>0145-305X</issn><eissn>1879-0089</eissn><abstract>Tuberculosis remains a major global health challenge. To gain information about genes important for defense against tuberculosis, we used a well-established tuberculosis model; Mycobacterium marinum infection in adult zebrafish. To characterize the immunological response to mycobacterial infection at 14 days post infection, we performed a whole-genome level transcriptome analysis using cells from kidney, the main hematopoietic organ of adult zebrafish. Among the upregulated genes, those associated with immune signaling and regulation formed the largest category, whereas the largest group of downregulated genes had a metabolic role. We also performed a forward genetic screen in adult zebrafish and identified a fish line with severely impaired survival during chronic mycobacterial infection. Based on transcriptome analysis, these fish have decreased expression of several immunological genes. Taken together, these results give new information about the genes involved in the defense against mycobacterial infection in zebrafish.
•201 genes were up- or downregulated at 14 days post zebrafish M. marinum infection.•Among upregulated genes, innate immune processes were most enriched.•Among downregulated genes, metabolic processes were most enriched.•We identified a mutant line with impaired resistance against M. marinum infection.•The mutants display deficiency in T cell activation and inflammasome function.</abstract><cop>OXFORD</cop><pub>Elsevier Ltd</pub><pmid>31626817</pmid><doi>10.1016/j.dci.2019.103523</doi><tpages>14</tpages><orcidid>https://orcid.org/0000-0001-6623-844X</orcidid><orcidid>https://orcid.org/0000-0001-6956-2843</orcidid><orcidid>https://orcid.org/0000-0003-3750-2779</orcidid><orcidid>https://orcid.org/0000-0002-6145-6875</orcidid><orcidid>https://orcid.org/0000-0001-9195-0797</orcidid><orcidid>https://orcid.org/0000-0001-5555-3815</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0145-305X |
| ispartof | Developmental and comparative immunology, 2020-02, Vol.103, p.103523-103523, Article 103523 |
| issn | 0145-305X 1879-0089 |
| language | eng |
| recordid | cdi_pubmed_primary_31626817 |
| source | Web of Science - Science Citation Index Expanded - 2020<img src="https://exlibris-pub.s3.amazonaws.com/fromwos-v2.jpg" />; Access via ScienceDirect (Elsevier) |
| subjects | Chronic infection Danio rerio Fisheries Forward genetic screen Gene expression Genes Genetic screening Genomes Global health Immune response Immune system Immunology Infections Life Sciences & Biomedicine Mycobacterium marinum Science & Technology Transcriptome analysis Tuberculosis Veterinary Sciences Zebrafish Zoology |
| title | Characterization of immune response against Mycobacterium marinum infection in the main hematopoietic organ of adult zebrafish (Danio rerio) |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-11T19%3A15%3A18IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Characterization%20of%20immune%20response%20against%20Mycobacterium%20marinum%20infection%20in%20the%20main%20hematopoietic%20organ%20of%20adult%20zebrafish%20(Danio%20rerio)&rft.jtitle=Developmental%20and%20comparative%20immunology&rft.au=Harjula,%20Sanna-Kaisa%20E.&rft.date=2020-02&rft.volume=103&rft.spage=103523&rft.epage=103523&rft.pages=103523-103523&rft.artnum=103523&rft.issn=0145-305X&rft.eissn=1879-0089&rft_id=info:doi/10.1016/j.dci.2019.103523&rft_dat=%3Cproquest_pubme%3E2307127699%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2344525982&rft_id=info:pmid/31626817&rft_els_id=S0145305X19304434&rfr_iscdi=true |