Evaluating the in vitro efficacy of gatifloxacin, levofloxacin and gentamicin against Acanthamoeba cysts
Purpose To evaluate the in vitro efficacy of three commercial ophthalmic solutions (gatifloxacin, levofloxacin and gentamicin) against cysts of Acanthamoeba species. Design Experimental study Methods Acanthamoeba cysts belonging to genotypes T3, T4 and T5 were incubated with three ophthalmic solutio...
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| Veröffentlicht in: | International ophthalmology 2020-02, Vol.40 (2), p.361-368 |
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| creator | Thongseesuksai, Thaksaporn Wongwai, Phanthipha Boonmars, Thidarut Sanpool, Oranuch Laummaunwai, Porntip |
| description | Purpose
To evaluate the in vitro efficacy of three commercial ophthalmic solutions (gatifloxacin, levofloxacin and gentamicin) against cysts of
Acanthamoeba
species.
Design
Experimental study
Methods
Acanthamoeba
cysts belonging to genotypes T3, T4 and T5 were incubated with three ophthalmic solutions for different periods of time; 1, 24, 48 and 72 h at 37 °C. After incubation, treated cysts were stained with trypan blue and counted to express the percent of growth inhibition. Additionally, the viability of treated cysts was assessed by culturing them in PYG medium at 30 °C for 72 h as well as on non-nutrient agar plates at 30 °C for 1 month.
Results
Acanthamoeba
cysts of all genotypes were susceptible to gentamicin and gatifloxacin after exposure for 1 h and 24 h, respectively, and for levofloxacin, cysts of all genotypes were resistant to levofloxacin even after 72 h of incubation. Gentamicin and gatifloxacin showed statistically highly significant difference (
P
|
| doi_str_mv | 10.1007/s10792-019-01188-4 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmed_primary_31587129</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2360707101</sourcerecordid><originalsourceid>FETCH-LOGICAL-c375t-b189b9458343130a1abb8aea9ef36e767b1c57abaae83b80c6c82ec83cfd54a43</originalsourceid><addsrcrecordid>eNqNkU9v1DAQxS0EokvhC3BAlrggQcCO49g-VqvyR6rEBc7R2DvJukrsEjsL--3xNm2ROCAOlj3yb57ezCPkJWfvOWPqQ-JMmbpi3JTDta6aR2TDpRJV3Qr2mGwYb2UlFeNn5FlK14wxo0z7lJwJLrXitdmQ_eUBxgWyDwPNe6Q-0IPPc6TY996BO9LY06H892P8Bc6Hd3TEQ7yvKIQdHTBkmPxtOYAPKdMLByHvYYpogbpjyuk5edLDmPDF3X1Ovn-8_Lb9XF19_fRle3FVOaFkrizXxppGatEILhhwsFYDgsFetKhaZbmTCiwAamE1c63TNTotXL-TDTTinLxZdW_m-GPBlLvJJ4fjCAHjkrpasLIqI6Qs6Ou_0Ou4zKG4K1TLFFOc8ULVK-XmmNKMfXcz-wnmY8dZd8qhW3PoSg7dbQ7dycWrO-nFTrh7aLlffAHersBPtLFPzmNw-ICVpCQ3tWh0ebGTnP5_eutzySuGbVxCLq1ibU0FDwPOf4b8h__f_0y04g</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2360707101</pqid></control><display><type>article</type><title>Evaluating the in vitro efficacy of gatifloxacin, levofloxacin and gentamicin against Acanthamoeba cysts</title><source>MEDLINE</source><source>Springer Online Journals Complete</source><source>Web of Science - Science Citation Index Expanded - 2020<img src="https://exlibris-pub.s3.amazonaws.com/fromwos-v2.jpg" /></source><creator>Thongseesuksai, Thaksaporn ; Wongwai, Phanthipha ; Boonmars, Thidarut ; Sanpool, Oranuch ; Laummaunwai, Porntip</creator><creatorcontrib>Thongseesuksai, Thaksaporn ; Wongwai, Phanthipha ; Boonmars, Thidarut ; Sanpool, Oranuch ; Laummaunwai, Porntip</creatorcontrib><description>Purpose
To evaluate the in vitro efficacy of three commercial ophthalmic solutions (gatifloxacin, levofloxacin and gentamicin) against cysts of
Acanthamoeba
species.
Design
Experimental study
Methods
Acanthamoeba
cysts belonging to genotypes T3, T4 and T5 were incubated with three ophthalmic solutions for different periods of time; 1, 24, 48 and 72 h at 37 °C. After incubation, treated cysts were stained with trypan blue and counted to express the percent of growth inhibition. Additionally, the viability of treated cysts was assessed by culturing them in PYG medium at 30 °C for 72 h as well as on non-nutrient agar plates at 30 °C for 1 month.
Results
Acanthamoeba
cysts of all genotypes were susceptible to gentamicin and gatifloxacin after exposure for 1 h and 24 h, respectively, and for levofloxacin, cysts of all genotypes were resistant to levofloxacin even after 72 h of incubation. Gentamicin and gatifloxacin showed statistically highly significant difference (
P
< 0.001), and levofloxacin showed statistically significant difference (
P
< 0.05) in comparison to non-treated control.
Conclusions
Gentamicin and gatifloxacin were highly effective against
Acanthamoeba
cysts. Although our results should be confirmed in animal models, this result will guide the choice of the appropriate ophthalmic drugs for early treatment of eye infection caused by
Acanthamoeba
spp.</description><identifier>ISSN: 0165-5701</identifier><identifier>EISSN: 1573-2630</identifier><identifier>DOI: 10.1007/s10792-019-01188-4</identifier><identifier>PMID: 31587129</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Acanthamoeba ; Acanthamoeba - drug effects ; Acanthamoeba - isolation & purification ; Amebiasis - drug therapy ; Animal models ; Animals ; Antibiotics ; Cysts ; Evaluation ; Eye Infections, Parasitic - drug therapy ; Gatifloxacin ; Gatifloxacin - pharmacology ; Genotypes ; Gentamicin ; Gentamicins - pharmacology ; Humans ; Incubation ; Levofloxacin ; Levofloxacin - pharmacology ; Life Sciences & Biomedicine ; Medicine ; Medicine & Public Health ; Ophthalmic Solutions ; Ophthalmology ; Original Paper ; Science & Technology ; Statistical analysis ; Viability</subject><ispartof>International ophthalmology, 2020-02, Vol.40 (2), p.361-368</ispartof><rights>Springer Nature B.V. 2019</rights><rights>International Ophthalmology is a copyright of Springer, (2019). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>true</woscitedreferencessubscribed><woscitedreferencescount>2</woscitedreferencescount><woscitedreferencesoriginalsourcerecordid>wos000519234800004</woscitedreferencesoriginalsourcerecordid><citedby>FETCH-LOGICAL-c375t-b189b9458343130a1abb8aea9ef36e767b1c57abaae83b80c6c82ec83cfd54a43</citedby><cites>FETCH-LOGICAL-c375t-b189b9458343130a1abb8aea9ef36e767b1c57abaae83b80c6c82ec83cfd54a43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10792-019-01188-4$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10792-019-01188-4$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>315,781,785,27929,27930,28253,41493,42562,51324</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31587129$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Thongseesuksai, Thaksaporn</creatorcontrib><creatorcontrib>Wongwai, Phanthipha</creatorcontrib><creatorcontrib>Boonmars, Thidarut</creatorcontrib><creatorcontrib>Sanpool, Oranuch</creatorcontrib><creatorcontrib>Laummaunwai, Porntip</creatorcontrib><title>Evaluating the in vitro efficacy of gatifloxacin, levofloxacin and gentamicin against Acanthamoeba cysts</title><title>International ophthalmology</title><addtitle>Int Ophthalmol</addtitle><addtitle>INT OPHTHALMOL</addtitle><addtitle>Int Ophthalmol</addtitle><description>Purpose
To evaluate the in vitro efficacy of three commercial ophthalmic solutions (gatifloxacin, levofloxacin and gentamicin) against cysts of
Acanthamoeba
species.
Design
Experimental study
Methods
Acanthamoeba
cysts belonging to genotypes T3, T4 and T5 were incubated with three ophthalmic solutions for different periods of time; 1, 24, 48 and 72 h at 37 °C. After incubation, treated cysts were stained with trypan blue and counted to express the percent of growth inhibition. Additionally, the viability of treated cysts was assessed by culturing them in PYG medium at 30 °C for 72 h as well as on non-nutrient agar plates at 30 °C for 1 month.
Results
Acanthamoeba
cysts of all genotypes were susceptible to gentamicin and gatifloxacin after exposure for 1 h and 24 h, respectively, and for levofloxacin, cysts of all genotypes were resistant to levofloxacin even after 72 h of incubation. Gentamicin and gatifloxacin showed statistically highly significant difference (
P
< 0.001), and levofloxacin showed statistically significant difference (
P
< 0.05) in comparison to non-treated control.
Conclusions
Gentamicin and gatifloxacin were highly effective against
Acanthamoeba
cysts. Although our results should be confirmed in animal models, this result will guide the choice of the appropriate ophthalmic drugs for early treatment of eye infection caused by
Acanthamoeba
spp.</description><subject>Acanthamoeba</subject><subject>Acanthamoeba - drug effects</subject><subject>Acanthamoeba - isolation & purification</subject><subject>Amebiasis - drug therapy</subject><subject>Animal models</subject><subject>Animals</subject><subject>Antibiotics</subject><subject>Cysts</subject><subject>Evaluation</subject><subject>Eye Infections, Parasitic - drug therapy</subject><subject>Gatifloxacin</subject><subject>Gatifloxacin - pharmacology</subject><subject>Genotypes</subject><subject>Gentamicin</subject><subject>Gentamicins - pharmacology</subject><subject>Humans</subject><subject>Incubation</subject><subject>Levofloxacin</subject><subject>Levofloxacin - pharmacology</subject><subject>Life Sciences & Biomedicine</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Ophthalmic Solutions</subject><subject>Ophthalmology</subject><subject>Original Paper</subject><subject>Science & Technology</subject><subject>Statistical analysis</subject><subject>Viability</subject><issn>0165-5701</issn><issn>1573-2630</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>AOWDO</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNqNkU9v1DAQxS0EokvhC3BAlrggQcCO49g-VqvyR6rEBc7R2DvJukrsEjsL--3xNm2ROCAOlj3yb57ezCPkJWfvOWPqQ-JMmbpi3JTDta6aR2TDpRJV3Qr2mGwYb2UlFeNn5FlK14wxo0z7lJwJLrXitdmQ_eUBxgWyDwPNe6Q-0IPPc6TY996BO9LY06H892P8Bc6Hd3TEQ7yvKIQdHTBkmPxtOYAPKdMLByHvYYpogbpjyuk5edLDmPDF3X1Ovn-8_Lb9XF19_fRle3FVOaFkrizXxppGatEILhhwsFYDgsFetKhaZbmTCiwAamE1c63TNTotXL-TDTTinLxZdW_m-GPBlLvJJ4fjCAHjkrpasLIqI6Qs6Ou_0Ou4zKG4K1TLFFOc8ULVK-XmmNKMfXcz-wnmY8dZd8qhW3PoSg7dbQ7dycWrO-nFTrh7aLlffAHersBPtLFPzmNw-ICVpCQ3tWh0ebGTnP5_eutzySuGbVxCLq1ibU0FDwPOf4b8h__f_0y04g</recordid><startdate>20200201</startdate><enddate>20200201</enddate><creator>Thongseesuksai, Thaksaporn</creator><creator>Wongwai, Phanthipha</creator><creator>Boonmars, Thidarut</creator><creator>Sanpool, Oranuch</creator><creator>Laummaunwai, Porntip</creator><general>Springer Netherlands</general><general>Springer Nature</general><general>Springer Nature B.V</general><scope>AOWDO</scope><scope>BLEPL</scope><scope>DTL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20200201</creationdate><title>Evaluating the in vitro efficacy of gatifloxacin, levofloxacin and gentamicin against Acanthamoeba cysts</title><author>Thongseesuksai, Thaksaporn ; Wongwai, Phanthipha ; Boonmars, Thidarut ; Sanpool, Oranuch ; Laummaunwai, Porntip</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-b189b9458343130a1abb8aea9ef36e767b1c57abaae83b80c6c82ec83cfd54a43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Acanthamoeba</topic><topic>Acanthamoeba - drug effects</topic><topic>Acanthamoeba - isolation & purification</topic><topic>Amebiasis - drug therapy</topic><topic>Animal models</topic><topic>Animals</topic><topic>Antibiotics</topic><topic>Cysts</topic><topic>Evaluation</topic><topic>Eye Infections, Parasitic - drug therapy</topic><topic>Gatifloxacin</topic><topic>Gatifloxacin - pharmacology</topic><topic>Genotypes</topic><topic>Gentamicin</topic><topic>Gentamicins - pharmacology</topic><topic>Humans</topic><topic>Incubation</topic><topic>Levofloxacin</topic><topic>Levofloxacin - pharmacology</topic><topic>Life Sciences & Biomedicine</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Ophthalmic Solutions</topic><topic>Ophthalmology</topic><topic>Original Paper</topic><topic>Science & Technology</topic><topic>Statistical analysis</topic><topic>Viability</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Thongseesuksai, Thaksaporn</creatorcontrib><creatorcontrib>Wongwai, Phanthipha</creatorcontrib><creatorcontrib>Boonmars, Thidarut</creatorcontrib><creatorcontrib>Sanpool, Oranuch</creatorcontrib><creatorcontrib>Laummaunwai, Porntip</creatorcontrib><collection>Web of Science - Science Citation Index Expanded - 2020</collection><collection>Web of Science Core Collection</collection><collection>Science Citation Index Expanded</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>International ophthalmology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Thongseesuksai, Thaksaporn</au><au>Wongwai, Phanthipha</au><au>Boonmars, Thidarut</au><au>Sanpool, Oranuch</au><au>Laummaunwai, Porntip</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluating the in vitro efficacy of gatifloxacin, levofloxacin and gentamicin against Acanthamoeba cysts</atitle><jtitle>International ophthalmology</jtitle><stitle>Int Ophthalmol</stitle><stitle>INT OPHTHALMOL</stitle><addtitle>Int Ophthalmol</addtitle><date>2020-02-01</date><risdate>2020</risdate><volume>40</volume><issue>2</issue><spage>361</spage><epage>368</epage><pages>361-368</pages><issn>0165-5701</issn><eissn>1573-2630</eissn><abstract>Purpose
To evaluate the in vitro efficacy of three commercial ophthalmic solutions (gatifloxacin, levofloxacin and gentamicin) against cysts of
Acanthamoeba
species.
Design
Experimental study
Methods
Acanthamoeba
cysts belonging to genotypes T3, T4 and T5 were incubated with three ophthalmic solutions for different periods of time; 1, 24, 48 and 72 h at 37 °C. After incubation, treated cysts were stained with trypan blue and counted to express the percent of growth inhibition. Additionally, the viability of treated cysts was assessed by culturing them in PYG medium at 30 °C for 72 h as well as on non-nutrient agar plates at 30 °C for 1 month.
Results
Acanthamoeba
cysts of all genotypes were susceptible to gentamicin and gatifloxacin after exposure for 1 h and 24 h, respectively, and for levofloxacin, cysts of all genotypes were resistant to levofloxacin even after 72 h of incubation. Gentamicin and gatifloxacin showed statistically highly significant difference (
P
< 0.001), and levofloxacin showed statistically significant difference (
P
< 0.05) in comparison to non-treated control.
Conclusions
Gentamicin and gatifloxacin were highly effective against
Acanthamoeba
cysts. Although our results should be confirmed in animal models, this result will guide the choice of the appropriate ophthalmic drugs for early treatment of eye infection caused by
Acanthamoeba
spp.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>31587129</pmid><doi>10.1007/s10792-019-01188-4</doi><tpages>8</tpages></addata></record> |
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| ispartof | International ophthalmology, 2020-02, Vol.40 (2), p.361-368 |
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| subjects | Acanthamoeba Acanthamoeba - drug effects Acanthamoeba - isolation & purification Amebiasis - drug therapy Animal models Animals Antibiotics Cysts Evaluation Eye Infections, Parasitic - drug therapy Gatifloxacin Gatifloxacin - pharmacology Genotypes Gentamicin Gentamicins - pharmacology Humans Incubation Levofloxacin Levofloxacin - pharmacology Life Sciences & Biomedicine Medicine Medicine & Public Health Ophthalmic Solutions Ophthalmology Original Paper Science & Technology Statistical analysis Viability |
| title | Evaluating the in vitro efficacy of gatifloxacin, levofloxacin and gentamicin against Acanthamoeba cysts |
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