Pseudomonas daroniae sp. nov. and Pseudomonas dryadis sp. nov., isolated from pedunculate oak affected by acute oak decline in the UK
Twenty-two cream-coloured bacterial strains were isolated from oak trees affected by acute oak decline (AOD) in Southern England. Isolates were Gram-negative, motile, slightly curved rods, aerobic, non-spore-forming, catalase positive and oxidase positive. 16S rRNA gene sequence analysis placed the...
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| Veröffentlicht in: | International journal of systematic and evolutionary microbiology 2019-11, Vol.69 (11), p.3368 |
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| container_title | International journal of systematic and evolutionary microbiology |
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| creator | Bueno-Gonzalez, Victoria Brady, Carrie Denman, Sandra Plummer, Sarah Allainguillaume, Joel Arnold, Dawn |
| description | Twenty-two cream-coloured bacterial strains were isolated from oak trees affected by acute oak decline (AOD) in Southern England. Isolates were Gram-negative, motile, slightly curved rods, aerobic, non-spore-forming, catalase positive and oxidase positive. 16S rRNA gene sequence analysis placed the strains in two separate phylogenetic clusters in the
group, with
as the closest phylogenetic relative. Multilocus sequence analyses of the
,
and
genes supported the delineation of the strains into two separate taxa, which could be differentiated phenotypically and chemotaxonomically from each other, and their closest relatives. Average nucleotide identity and
DNA-DNA hybridization values revealed percentages of genome similarity below the species threshold (95 and 70 %, respectively) between the two taxa and the closest relatives, confirming their novel species status. Therefore, on the basis of this polyphasic approach we propose two novel
species,
sp. nov. (type strain FRB 228
=LMG 31087
=NCPPB 4672
) and
sp. nov. (type strain FRB 230
=LMG 31087
=NCPPB 4673
). |
| doi_str_mv | 10.1099/ijsem.0.003615 |
| format | Article |
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group, with
as the closest phylogenetic relative. Multilocus sequence analyses of the
,
and
genes supported the delineation of the strains into two separate taxa, which could be differentiated phenotypically and chemotaxonomically from each other, and their closest relatives. Average nucleotide identity and
DNA-DNA hybridization values revealed percentages of genome similarity below the species threshold (95 and 70 %, respectively) between the two taxa and the closest relatives, confirming their novel species status. Therefore, on the basis of this polyphasic approach we propose two novel
species,
sp. nov. (type strain FRB 228
=LMG 31087
=NCPPB 4672
) and
sp. nov. (type strain FRB 230
=LMG 31087
=NCPPB 4673
).</description><identifier>EISSN: 1466-5034</identifier><identifier>DOI: 10.1099/ijsem.0.003615</identifier><identifier>PMID: 31391144</identifier><language>eng</language><publisher>England</publisher><subject>Bacterial Typing Techniques ; Base Composition ; DNA, Bacterial - genetics ; Fatty Acids - chemistry ; Genes, Bacterial ; Multilocus Sequence Typing ; Nucleic Acid Hybridization ; Phylogeny ; Plant Diseases - microbiology ; Pseudomonas - classification ; Pseudomonas - isolation & purification ; Quercus - microbiology ; RNA, Ribosomal, 16S - genetics ; Sequence Analysis, DNA ; United Kingdom</subject><ispartof>International journal of systematic and evolutionary microbiology, 2019-11, Vol.69 (11), p.3368</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31391144$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bueno-Gonzalez, Victoria</creatorcontrib><creatorcontrib>Brady, Carrie</creatorcontrib><creatorcontrib>Denman, Sandra</creatorcontrib><creatorcontrib>Plummer, Sarah</creatorcontrib><creatorcontrib>Allainguillaume, Joel</creatorcontrib><creatorcontrib>Arnold, Dawn</creatorcontrib><title>Pseudomonas daroniae sp. nov. and Pseudomonas dryadis sp. nov., isolated from pedunculate oak affected by acute oak decline in the UK</title><title>International journal of systematic and evolutionary microbiology</title><addtitle>Int J Syst Evol Microbiol</addtitle><description>Twenty-two cream-coloured bacterial strains were isolated from oak trees affected by acute oak decline (AOD) in Southern England. Isolates were Gram-negative, motile, slightly curved rods, aerobic, non-spore-forming, catalase positive and oxidase positive. 16S rRNA gene sequence analysis placed the strains in two separate phylogenetic clusters in the
group, with
as the closest phylogenetic relative. Multilocus sequence analyses of the
,
and
genes supported the delineation of the strains into two separate taxa, which could be differentiated phenotypically and chemotaxonomically from each other, and their closest relatives. Average nucleotide identity and
DNA-DNA hybridization values revealed percentages of genome similarity below the species threshold (95 and 70 %, respectively) between the two taxa and the closest relatives, confirming their novel species status. Therefore, on the basis of this polyphasic approach we propose two novel
species,
sp. nov. (type strain FRB 228
=LMG 31087
=NCPPB 4672
) and
sp. nov. (type strain FRB 230
=LMG 31087
=NCPPB 4673
).</description><subject>Bacterial Typing Techniques</subject><subject>Base Composition</subject><subject>DNA, Bacterial - genetics</subject><subject>Fatty Acids - chemistry</subject><subject>Genes, Bacterial</subject><subject>Multilocus Sequence Typing</subject><subject>Nucleic Acid Hybridization</subject><subject>Phylogeny</subject><subject>Plant Diseases - microbiology</subject><subject>Pseudomonas - classification</subject><subject>Pseudomonas - isolation & purification</subject><subject>Quercus - microbiology</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Sequence Analysis, DNA</subject><subject>United Kingdom</subject><issn>1466-5034</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkMtKAzEYhYMgtla3LuV_AGf8k0nSmaUUb1jQRV2XXDG1kwyTGaEP4HtrsQquDnzn4ywOIRcUS4pNcx022bUlloiVpOKITCmXshBY8Qk5zXmD-A0QT8ikolVDKedT8vmS3WhTm6LKYFWfYlAOcldCTB8lqGjhn9HvlA35T7iCkNNWDc6C71MLnbNjNOOeQFLvoLx3Zt_qHSgzHqh1ZhuigxBheHPw-nRGjr3aZnd-yBlZ3d2uFg_F8vn-cXGzLDqK9VAoTpnmtNZMMGE48_M5Sm_Q1FwKVTfSMIHaCWq4mDOmPbW8oV4L4SiTWM3I5c9sN-rW2XXXh1b1u_XvHdUXMedgqA</recordid><startdate>201911</startdate><enddate>201911</enddate><creator>Bueno-Gonzalez, Victoria</creator><creator>Brady, Carrie</creator><creator>Denman, Sandra</creator><creator>Plummer, Sarah</creator><creator>Allainguillaume, Joel</creator><creator>Arnold, Dawn</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>201911</creationdate><title>Pseudomonas daroniae sp. nov. and Pseudomonas dryadis sp. nov., isolated from pedunculate oak affected by acute oak decline in the UK</title><author>Bueno-Gonzalez, Victoria ; Brady, Carrie ; Denman, Sandra ; Plummer, Sarah ; Allainguillaume, Joel ; Arnold, Dawn</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p108t-a412b418b2525c42f7706fc0c8465a896c250be51c45722bf1d491fb55e12603</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Bacterial Typing Techniques</topic><topic>Base Composition</topic><topic>DNA, Bacterial - genetics</topic><topic>Fatty Acids - chemistry</topic><topic>Genes, Bacterial</topic><topic>Multilocus Sequence Typing</topic><topic>Nucleic Acid Hybridization</topic><topic>Phylogeny</topic><topic>Plant Diseases - microbiology</topic><topic>Pseudomonas - classification</topic><topic>Pseudomonas - isolation & purification</topic><topic>Quercus - microbiology</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Sequence Analysis, DNA</topic><topic>United Kingdom</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bueno-Gonzalez, Victoria</creatorcontrib><creatorcontrib>Brady, Carrie</creatorcontrib><creatorcontrib>Denman, Sandra</creatorcontrib><creatorcontrib>Plummer, Sarah</creatorcontrib><creatorcontrib>Allainguillaume, Joel</creatorcontrib><creatorcontrib>Arnold, Dawn</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>International journal of systematic and evolutionary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bueno-Gonzalez, Victoria</au><au>Brady, Carrie</au><au>Denman, Sandra</au><au>Plummer, Sarah</au><au>Allainguillaume, Joel</au><au>Arnold, Dawn</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pseudomonas daroniae sp. nov. and Pseudomonas dryadis sp. nov., isolated from pedunculate oak affected by acute oak decline in the UK</atitle><jtitle>International journal of systematic and evolutionary microbiology</jtitle><addtitle>Int J Syst Evol Microbiol</addtitle><date>2019-11</date><risdate>2019</risdate><volume>69</volume><issue>11</issue><spage>3368</spage><pages>3368-</pages><eissn>1466-5034</eissn><abstract>Twenty-two cream-coloured bacterial strains were isolated from oak trees affected by acute oak decline (AOD) in Southern England. Isolates were Gram-negative, motile, slightly curved rods, aerobic, non-spore-forming, catalase positive and oxidase positive. 16S rRNA gene sequence analysis placed the strains in two separate phylogenetic clusters in the
group, with
as the closest phylogenetic relative. Multilocus sequence analyses of the
,
and
genes supported the delineation of the strains into two separate taxa, which could be differentiated phenotypically and chemotaxonomically from each other, and their closest relatives. Average nucleotide identity and
DNA-DNA hybridization values revealed percentages of genome similarity below the species threshold (95 and 70 %, respectively) between the two taxa and the closest relatives, confirming their novel species status. Therefore, on the basis of this polyphasic approach we propose two novel
species,
sp. nov. (type strain FRB 228
=LMG 31087
=NCPPB 4672
) and
sp. nov. (type strain FRB 230
=LMG 31087
=NCPPB 4673
).</abstract><cop>England</cop><pmid>31391144</pmid><doi>10.1099/ijsem.0.003615</doi></addata></record> |
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| ispartof | International journal of systematic and evolutionary microbiology, 2019-11, Vol.69 (11), p.3368 |
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| language | eng |
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| source | MEDLINE; Microbiology Society; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Bacterial Typing Techniques Base Composition DNA, Bacterial - genetics Fatty Acids - chemistry Genes, Bacterial Multilocus Sequence Typing Nucleic Acid Hybridization Phylogeny Plant Diseases - microbiology Pseudomonas - classification Pseudomonas - isolation & purification Quercus - microbiology RNA, Ribosomal, 16S - genetics Sequence Analysis, DNA United Kingdom |
| title | Pseudomonas daroniae sp. nov. and Pseudomonas dryadis sp. nov., isolated from pedunculate oak affected by acute oak decline in the UK |
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