Enhanced bone regeneration capability of chitosan sponge coated with TiO 2 nanoparticles
Chitosan has been a popular option for tissue engineering, however exhibits limited function for bone regeneration due to its low mechanical robustness and non-osteogenic inductivity. Here we hybridized chitosan with TiO nanoparticles to improve its bone regeneration capability. Morphology and cryst...
Gespeichert in:
| Veröffentlicht in: | Biotechnology reports (Amsterdam, Netherlands) Netherlands), 2019-12, Vol.24, p.e00350 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | e00350 |
| container_title | Biotechnology reports (Amsterdam, Netherlands) |
| container_volume | 24 |
| creator | Ikono, Radyum Li, Ni Pratama, Nanda Hendra Vibriani, Agnia Yuniarni, Diah Retno Luthfansyah, Muhammad Bachtiar, Boy Muchlis Bachtiar, Endang Winiati Mulia, Kamarza Nasikin, Mohammad Kagami, Hideaki Li, Xianqi Mardliyati, Etik Rochman, Nurul Taufiqu Nagamura-Inoue, Tokiko Tojo, Arinobu |
| description | Chitosan has been a popular option for tissue engineering, however exhibits limited function for bone regeneration due to its low mechanical robustness and non-osteogenic inductivity. Here we hybridized chitosan with TiO
nanoparticles to improve its bone regeneration capability. Morphology and crystallographic analysis showed that TiO
nanoparticles in anatase-type were distributed evenly on the surface of the chitosan sponges. Degradation test showed a significant effect of TiO
nanoparticles addition in retaining its integrity. Biomineralization assay using simulated body fluid showed apatite formation in sponges surface as denoted by PO
band observed in FTIR results. qPCR analysis supported chitosan - TiO
sponges in bone regeneration capability as indicated by DMP1 and OCN gene upregulation in TiO
treated group. Finally, cytotoxicity analysis supported the fact that TiO
nanoparticles added sponges were proved to be biocompatible. Results suggest that chitosan-50% TiO
nanoparticles sponges could be a potential novel scaffold for bone tissue engineering. |
| format | Article |
| fullrecord | <record><control><sourceid>pubmed</sourceid><recordid>TN_cdi_pubmed_primary_31304101</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>31304101</sourcerecordid><originalsourceid>FETCH-pubmed_primary_313041013</originalsourceid><addsrcrecordid>eNqFjr0KwjAURoMoKuoryH0BIemP4iyKm4tDt3Kb3raR9iYkEfHtdVBwc_rOcA58IzFPEpVvpNoV4x-eiVUINymlSjOZb_dTMUtVKjMl1VwUR-6QNdVQWSbw1BKTx2gsg0aHlelNfIJtQHcm2oAMwVluCbTF-M4eJnZwNRdIgJGtQx-N7iksxaTBPtDqswuxPh2vh_PG3auB6tJ5M6B_lt8r6V_hBR5pQdI</addsrcrecordid><sourcetype>Index Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Enhanced bone regeneration capability of chitosan sponge coated with TiO 2 nanoparticles</title><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Ikono, Radyum ; Li, Ni ; Pratama, Nanda Hendra ; Vibriani, Agnia ; Yuniarni, Diah Retno ; Luthfansyah, Muhammad ; Bachtiar, Boy Muchlis ; Bachtiar, Endang Winiati ; Mulia, Kamarza ; Nasikin, Mohammad ; Kagami, Hideaki ; Li, Xianqi ; Mardliyati, Etik ; Rochman, Nurul Taufiqu ; Nagamura-Inoue, Tokiko ; Tojo, Arinobu</creator><creatorcontrib>Ikono, Radyum ; Li, Ni ; Pratama, Nanda Hendra ; Vibriani, Agnia ; Yuniarni, Diah Retno ; Luthfansyah, Muhammad ; Bachtiar, Boy Muchlis ; Bachtiar, Endang Winiati ; Mulia, Kamarza ; Nasikin, Mohammad ; Kagami, Hideaki ; Li, Xianqi ; Mardliyati, Etik ; Rochman, Nurul Taufiqu ; Nagamura-Inoue, Tokiko ; Tojo, Arinobu</creatorcontrib><description>Chitosan has been a popular option for tissue engineering, however exhibits limited function for bone regeneration due to its low mechanical robustness and non-osteogenic inductivity. Here we hybridized chitosan with TiO
nanoparticles to improve its bone regeneration capability. Morphology and crystallographic analysis showed that TiO
nanoparticles in anatase-type were distributed evenly on the surface of the chitosan sponges. Degradation test showed a significant effect of TiO
nanoparticles addition in retaining its integrity. Biomineralization assay using simulated body fluid showed apatite formation in sponges surface as denoted by PO
band observed in FTIR results. qPCR analysis supported chitosan - TiO
sponges in bone regeneration capability as indicated by DMP1 and OCN gene upregulation in TiO
treated group. Finally, cytotoxicity analysis supported the fact that TiO
nanoparticles added sponges were proved to be biocompatible. Results suggest that chitosan-50% TiO
nanoparticles sponges could be a potential novel scaffold for bone tissue engineering.</description><identifier>ISSN: 2215-017X</identifier><identifier>EISSN: 2215-017X</identifier><identifier>PMID: 31304101</identifier><language>eng</language><publisher>Netherlands</publisher><ispartof>Biotechnology reports (Amsterdam, Netherlands), 2019-12, Vol.24, p.e00350</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31304101$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ikono, Radyum</creatorcontrib><creatorcontrib>Li, Ni</creatorcontrib><creatorcontrib>Pratama, Nanda Hendra</creatorcontrib><creatorcontrib>Vibriani, Agnia</creatorcontrib><creatorcontrib>Yuniarni, Diah Retno</creatorcontrib><creatorcontrib>Luthfansyah, Muhammad</creatorcontrib><creatorcontrib>Bachtiar, Boy Muchlis</creatorcontrib><creatorcontrib>Bachtiar, Endang Winiati</creatorcontrib><creatorcontrib>Mulia, Kamarza</creatorcontrib><creatorcontrib>Nasikin, Mohammad</creatorcontrib><creatorcontrib>Kagami, Hideaki</creatorcontrib><creatorcontrib>Li, Xianqi</creatorcontrib><creatorcontrib>Mardliyati, Etik</creatorcontrib><creatorcontrib>Rochman, Nurul Taufiqu</creatorcontrib><creatorcontrib>Nagamura-Inoue, Tokiko</creatorcontrib><creatorcontrib>Tojo, Arinobu</creatorcontrib><title>Enhanced bone regeneration capability of chitosan sponge coated with TiO 2 nanoparticles</title><title>Biotechnology reports (Amsterdam, Netherlands)</title><addtitle>Biotechnol Rep (Amst)</addtitle><description>Chitosan has been a popular option for tissue engineering, however exhibits limited function for bone regeneration due to its low mechanical robustness and non-osteogenic inductivity. Here we hybridized chitosan with TiO
nanoparticles to improve its bone regeneration capability. Morphology and crystallographic analysis showed that TiO
nanoparticles in anatase-type were distributed evenly on the surface of the chitosan sponges. Degradation test showed a significant effect of TiO
nanoparticles addition in retaining its integrity. Biomineralization assay using simulated body fluid showed apatite formation in sponges surface as denoted by PO
band observed in FTIR results. qPCR analysis supported chitosan - TiO
sponges in bone regeneration capability as indicated by DMP1 and OCN gene upregulation in TiO
treated group. Finally, cytotoxicity analysis supported the fact that TiO
nanoparticles added sponges were proved to be biocompatible. Results suggest that chitosan-50% TiO
nanoparticles sponges could be a potential novel scaffold for bone tissue engineering.</description><issn>2215-017X</issn><issn>2215-017X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNqFjr0KwjAURoMoKuoryH0BIemP4iyKm4tDt3Kb3raR9iYkEfHtdVBwc_rOcA58IzFPEpVvpNoV4x-eiVUINymlSjOZb_dTMUtVKjMl1VwUR-6QNdVQWSbw1BKTx2gsg0aHlelNfIJtQHcm2oAMwVluCbTF-M4eJnZwNRdIgJGtQx-N7iksxaTBPtDqswuxPh2vh_PG3auB6tJ5M6B_lt8r6V_hBR5pQdI</recordid><startdate>201912</startdate><enddate>201912</enddate><creator>Ikono, Radyum</creator><creator>Li, Ni</creator><creator>Pratama, Nanda Hendra</creator><creator>Vibriani, Agnia</creator><creator>Yuniarni, Diah Retno</creator><creator>Luthfansyah, Muhammad</creator><creator>Bachtiar, Boy Muchlis</creator><creator>Bachtiar, Endang Winiati</creator><creator>Mulia, Kamarza</creator><creator>Nasikin, Mohammad</creator><creator>Kagami, Hideaki</creator><creator>Li, Xianqi</creator><creator>Mardliyati, Etik</creator><creator>Rochman, Nurul Taufiqu</creator><creator>Nagamura-Inoue, Tokiko</creator><creator>Tojo, Arinobu</creator><scope>NPM</scope></search><sort><creationdate>201912</creationdate><title>Enhanced bone regeneration capability of chitosan sponge coated with TiO 2 nanoparticles</title><author>Ikono, Radyum ; Li, Ni ; Pratama, Nanda Hendra ; Vibriani, Agnia ; Yuniarni, Diah Retno ; Luthfansyah, Muhammad ; Bachtiar, Boy Muchlis ; Bachtiar, Endang Winiati ; Mulia, Kamarza ; Nasikin, Mohammad ; Kagami, Hideaki ; Li, Xianqi ; Mardliyati, Etik ; Rochman, Nurul Taufiqu ; Nagamura-Inoue, Tokiko ; Tojo, Arinobu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-pubmed_primary_313041013</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ikono, Radyum</creatorcontrib><creatorcontrib>Li, Ni</creatorcontrib><creatorcontrib>Pratama, Nanda Hendra</creatorcontrib><creatorcontrib>Vibriani, Agnia</creatorcontrib><creatorcontrib>Yuniarni, Diah Retno</creatorcontrib><creatorcontrib>Luthfansyah, Muhammad</creatorcontrib><creatorcontrib>Bachtiar, Boy Muchlis</creatorcontrib><creatorcontrib>Bachtiar, Endang Winiati</creatorcontrib><creatorcontrib>Mulia, Kamarza</creatorcontrib><creatorcontrib>Nasikin, Mohammad</creatorcontrib><creatorcontrib>Kagami, Hideaki</creatorcontrib><creatorcontrib>Li, Xianqi</creatorcontrib><creatorcontrib>Mardliyati, Etik</creatorcontrib><creatorcontrib>Rochman, Nurul Taufiqu</creatorcontrib><creatorcontrib>Nagamura-Inoue, Tokiko</creatorcontrib><creatorcontrib>Tojo, Arinobu</creatorcontrib><collection>PubMed</collection><jtitle>Biotechnology reports (Amsterdam, Netherlands)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ikono, Radyum</au><au>Li, Ni</au><au>Pratama, Nanda Hendra</au><au>Vibriani, Agnia</au><au>Yuniarni, Diah Retno</au><au>Luthfansyah, Muhammad</au><au>Bachtiar, Boy Muchlis</au><au>Bachtiar, Endang Winiati</au><au>Mulia, Kamarza</au><au>Nasikin, Mohammad</au><au>Kagami, Hideaki</au><au>Li, Xianqi</au><au>Mardliyati, Etik</au><au>Rochman, Nurul Taufiqu</au><au>Nagamura-Inoue, Tokiko</au><au>Tojo, Arinobu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enhanced bone regeneration capability of chitosan sponge coated with TiO 2 nanoparticles</atitle><jtitle>Biotechnology reports (Amsterdam, Netherlands)</jtitle><addtitle>Biotechnol Rep (Amst)</addtitle><date>2019-12</date><risdate>2019</risdate><volume>24</volume><spage>e00350</spage><pages>e00350-</pages><issn>2215-017X</issn><eissn>2215-017X</eissn><abstract>Chitosan has been a popular option for tissue engineering, however exhibits limited function for bone regeneration due to its low mechanical robustness and non-osteogenic inductivity. Here we hybridized chitosan with TiO
nanoparticles to improve its bone regeneration capability. Morphology and crystallographic analysis showed that TiO
nanoparticles in anatase-type were distributed evenly on the surface of the chitosan sponges. Degradation test showed a significant effect of TiO
nanoparticles addition in retaining its integrity. Biomineralization assay using simulated body fluid showed apatite formation in sponges surface as denoted by PO
band observed in FTIR results. qPCR analysis supported chitosan - TiO
sponges in bone regeneration capability as indicated by DMP1 and OCN gene upregulation in TiO
treated group. Finally, cytotoxicity analysis supported the fact that TiO
nanoparticles added sponges were proved to be biocompatible. Results suggest that chitosan-50% TiO
nanoparticles sponges could be a potential novel scaffold for bone tissue engineering.</abstract><cop>Netherlands</cop><pmid>31304101</pmid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2215-017X |
| ispartof | Biotechnology reports (Amsterdam, Netherlands), 2019-12, Vol.24, p.e00350 |
| issn | 2215-017X 2215-017X |
| language | eng |
| recordid | cdi_pubmed_primary_31304101 |
| source | DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection |
| title | Enhanced bone regeneration capability of chitosan sponge coated with TiO 2 nanoparticles |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T11%3A13%3A13IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-pubmed&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Enhanced%20bone%20regeneration%20capability%20of%20chitosan%20sponge%20coated%20with%20TiO%202%20nanoparticles&rft.jtitle=Biotechnology%20reports%20(Amsterdam,%20Netherlands)&rft.au=Ikono,%20Radyum&rft.date=2019-12&rft.volume=24&rft.spage=e00350&rft.pages=e00350-&rft.issn=2215-017X&rft.eissn=2215-017X&rft_id=info:doi/&rft_dat=%3Cpubmed%3E31304101%3C/pubmed%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/31304101&rfr_iscdi=true |