Atopic Patients Show Increased Interleukin 4 Plasma Levels but the Degree of Elevation Is Not Sufficient to Upregulate Interleukin-4-Sensitive Genes
Background: Atopic diseases constitute a major health challenge for industrialized countries, and elevated levels of interleukin 4 (IL-4) frequently characterize these disorders. Previous in vitroanalyses have indicated that IL-4 strongly upregulates the expression of IL-4-sensitive genes in human m...
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| Veröffentlicht in: | Skin pharmacology and physiology 2019-07, Vol.32 (4), p.192-200 |
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| creator | Marbach-Breitrück, Eugenia Kalledat, Andrea Heydeck, Dagmar Stehling, Sabine Fluhr, Joachim W. Zuberbier, Torsten Kuhn, Hartmut |
| description | Background: Atopic diseases constitute a major health challenge for industrialized countries, and elevated levels of interleukin 4 (IL-4) frequently characterize these disorders. Previous in vitroanalyses have indicated that IL-4 strongly upregulates the expression of IL-4-sensitive genes in human monocytes. Objective: To explore whether similar expression alterations may contribute to the pathomechanisms of atopic diseases in vivo we carried out a small-scale case-control clinical study (n = 43), in which we quantified the plasma levels of IgE and IL-4 as well as the expression of selected IL-4-sensitive genes in blood leukocytes. Methods: 34 allergic patients suffering from allergic rhinitis (n = 11), atopic eczema (n = 11) and allergic asthma (n = 12) as well as 9 healthy control individuals were recruited. IgE and IL-4 plasma levels were determined by ELISA, and the expression of selected IL-4-sensitive gene products in blood leukocytes was quantified by qRT-PCR. In addition, the fatty acid oxygenase activity of isolated monocytes was measured by RP-HPLC analysis of the arachidonic acid oxygenation products (ex vivo activity assays). Results: We found that plasma levels of IgE and IL-4 were significantly elevated in atopic patients but the degree of elevation was not sufficient to upregulate the expression of the selected IL-4-sensitive genes in circulating leukocytes. Moreover, the arachidonic acid oxygenase activity of blood monocytes was not significantly altered in atopic patients. Conclusion: Our data suggest that the IL-4 plasma levels of atopic patients are not high enough to impact the expression of IL-4-sensitive genes. |
| doi_str_mv | 10.1159/000499431 |
| format | Article |
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Previous in vitroanalyses have indicated that IL-4 strongly upregulates the expression of IL-4-sensitive genes in human monocytes. Objective: To explore whether similar expression alterations may contribute to the pathomechanisms of atopic diseases in vivo we carried out a small-scale case-control clinical study (n = 43), in which we quantified the plasma levels of IgE and IL-4 as well as the expression of selected IL-4-sensitive genes in blood leukocytes. Methods: 34 allergic patients suffering from allergic rhinitis (n = 11), atopic eczema (n = 11) and allergic asthma (n = 12) as well as 9 healthy control individuals were recruited. IgE and IL-4 plasma levels were determined by ELISA, and the expression of selected IL-4-sensitive gene products in blood leukocytes was quantified by qRT-PCR. In addition, the fatty acid oxygenase activity of isolated monocytes was measured by RP-HPLC analysis of the arachidonic acid oxygenation products (ex vivo activity assays). Results: We found that plasma levels of IgE and IL-4 were significantly elevated in atopic patients but the degree of elevation was not sufficient to upregulate the expression of the selected IL-4-sensitive genes in circulating leukocytes. Moreover, the arachidonic acid oxygenase activity of blood monocytes was not significantly altered in atopic patients. Conclusion: Our data suggest that the IL-4 plasma levels of atopic patients are not high enough to impact the expression of IL-4-sensitive genes.</description><identifier>ISSN: 1660-5527</identifier><identifier>EISSN: 1660-5535</identifier><identifier>DOI: 10.1159/000499431</identifier><identifier>PMID: 31096247</identifier><language>eng</language><publisher>Basel, Switzerland: S. Karger AG</publisher><subject>Adult ; Asthma - blood ; Asthma - genetics ; Atopic dermatitis ; Case-Control Studies ; Dermatitis, Atopic - blood ; Dermatitis, Atopic - genetics ; Development and progression ; Enzyme-Linked Immunosorbent Assay ; Female ; Gene expression ; Genetic aspects ; Health aspects ; Humans ; Hypersensitivity, Immediate - blood ; Hypersensitivity, Immediate - genetics ; Immunoglobulin E - biosynthesis ; Interleukin-4 ; Interleukin-4 - biosynthesis ; Leukocytes - physiology ; Male ; Measurement ; Middle Aged ; Oxygenases - metabolism ; Physiological aspects ; Real-Time Polymerase Chain Reaction ; Research Article ; Rhinitis, Allergic - blood ; Rhinitis, Allergic - genetics ; Up-Regulation</subject><ispartof>Skin pharmacology and physiology, 2019-07, Vol.32 (4), p.192-200</ispartof><rights>2019 S. Karger AG, Basel</rights><rights>2019 S. Karger AG, Basel.</rights><rights>COPYRIGHT 2019 S. Karger AG</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c436t-5f37ae240ebe1ff45e3b9a8a5e2e9c0eb665bd8bf1e09c4df9d8cf805f6831ca3</citedby><cites>FETCH-LOGICAL-c436t-5f37ae240ebe1ff45e3b9a8a5e2e9c0eb665bd8bf1e09c4df9d8cf805f6831ca3</cites><orcidid>0000-0002-1466-8875 ; 0000-0003-3610-0698</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,2425,27907,27908</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31096247$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Marbach-Breitrück, Eugenia</creatorcontrib><creatorcontrib>Kalledat, Andrea</creatorcontrib><creatorcontrib>Heydeck, Dagmar</creatorcontrib><creatorcontrib>Stehling, Sabine</creatorcontrib><creatorcontrib>Fluhr, Joachim W.</creatorcontrib><creatorcontrib>Zuberbier, Torsten</creatorcontrib><creatorcontrib>Kuhn, Hartmut</creatorcontrib><title>Atopic Patients Show Increased Interleukin 4 Plasma Levels but the Degree of Elevation Is Not Sufficient to Upregulate Interleukin-4-Sensitive Genes</title><title>Skin pharmacology and physiology</title><addtitle>Skin Pharmacol Physiol</addtitle><description>Background: Atopic diseases constitute a major health challenge for industrialized countries, and elevated levels of interleukin 4 (IL-4) frequently characterize these disorders. Previous in vitroanalyses have indicated that IL-4 strongly upregulates the expression of IL-4-sensitive genes in human monocytes. Objective: To explore whether similar expression alterations may contribute to the pathomechanisms of atopic diseases in vivo we carried out a small-scale case-control clinical study (n = 43), in which we quantified the plasma levels of IgE and IL-4 as well as the expression of selected IL-4-sensitive genes in blood leukocytes. Methods: 34 allergic patients suffering from allergic rhinitis (n = 11), atopic eczema (n = 11) and allergic asthma (n = 12) as well as 9 healthy control individuals were recruited. IgE and IL-4 plasma levels were determined by ELISA, and the expression of selected IL-4-sensitive gene products in blood leukocytes was quantified by qRT-PCR. In addition, the fatty acid oxygenase activity of isolated monocytes was measured by RP-HPLC analysis of the arachidonic acid oxygenation products (ex vivo activity assays). Results: We found that plasma levels of IgE and IL-4 were significantly elevated in atopic patients but the degree of elevation was not sufficient to upregulate the expression of the selected IL-4-sensitive genes in circulating leukocytes. Moreover, the arachidonic acid oxygenase activity of blood monocytes was not significantly altered in atopic patients. Conclusion: Our data suggest that the IL-4 plasma levels of atopic patients are not high enough to impact the expression of IL-4-sensitive genes.</description><subject>Adult</subject><subject>Asthma - blood</subject><subject>Asthma - genetics</subject><subject>Atopic dermatitis</subject><subject>Case-Control Studies</subject><subject>Dermatitis, Atopic - blood</subject><subject>Dermatitis, Atopic - genetics</subject><subject>Development and progression</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Female</subject><subject>Gene expression</subject><subject>Genetic aspects</subject><subject>Health aspects</subject><subject>Humans</subject><subject>Hypersensitivity, Immediate - blood</subject><subject>Hypersensitivity, Immediate - genetics</subject><subject>Immunoglobulin E - biosynthesis</subject><subject>Interleukin-4</subject><subject>Interleukin-4 - biosynthesis</subject><subject>Leukocytes - physiology</subject><subject>Male</subject><subject>Measurement</subject><subject>Middle Aged</subject><subject>Oxygenases - metabolism</subject><subject>Physiological aspects</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Research Article</subject><subject>Rhinitis, Allergic - blood</subject><subject>Rhinitis, Allergic - genetics</subject><subject>Up-Regulation</subject><issn>1660-5527</issn><issn>1660-5535</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkUGL1DAUx4so7rp68C4S8KKHrkmapM1xWNd1YNCBcc8lTV9m47ZJTdIRv4cf2AwzjgpLDnk8fu_3HvyL4iXBl4Rw-R5jzKRkFXlUnBMhcMl5xR-falqfFc9i_IYxFTURT4uzimApKKvPi1-L5Cer0VolCy5FtLnzP9DS6QAqQp-rBGGA-d46xNB6UHFUaAU7GCLq5oTSHaAPsA0AyBt0PcAui7xDy4g--4Q2szFW780oeXQ7BdjOg0rwr7dk5QZctMnuAN2Ag_i8eGLUEOHF8b8obj9ef736VK6-3CyvFqtSs0qkkpuqVkAZhg6IMYxD1UnVKA4UpM5dIXjXN50hgKVmvZF9o02DuRFNRbSqLoq3B-8U_PcZYmpHGzUMg3Lg59hSWlHMmKxpRt8c0K0aoLXO-BSU3uPtQtCG1pJwlqnLB6j8ehit9g6Mzf3_Bt4dBnTwMQYw7RTsqMLPluB2n217yjazr4_Xzt0I_Yn8E-bfG-9V2EI4AZv1-qBop95k6tWD1HHLb8J1tDE</recordid><startdate>20190701</startdate><enddate>20190701</enddate><creator>Marbach-Breitrück, Eugenia</creator><creator>Kalledat, Andrea</creator><creator>Heydeck, Dagmar</creator><creator>Stehling, Sabine</creator><creator>Fluhr, Joachim W.</creator><creator>Zuberbier, Torsten</creator><creator>Kuhn, Hartmut</creator><general>S. Karger AG</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-1466-8875</orcidid><orcidid>https://orcid.org/0000-0003-3610-0698</orcidid></search><sort><creationdate>20190701</creationdate><title>Atopic Patients Show Increased Interleukin 4 Plasma Levels but the Degree of Elevation Is Not Sufficient to Upregulate Interleukin-4-Sensitive Genes</title><author>Marbach-Breitrück, Eugenia ; Kalledat, Andrea ; Heydeck, Dagmar ; Stehling, Sabine ; Fluhr, Joachim W. ; Zuberbier, Torsten ; Kuhn, Hartmut</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c436t-5f37ae240ebe1ff45e3b9a8a5e2e9c0eb665bd8bf1e09c4df9d8cf805f6831ca3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Adult</topic><topic>Asthma - blood</topic><topic>Asthma - genetics</topic><topic>Atopic dermatitis</topic><topic>Case-Control Studies</topic><topic>Dermatitis, Atopic - blood</topic><topic>Dermatitis, Atopic - genetics</topic><topic>Development and progression</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Female</topic><topic>Gene expression</topic><topic>Genetic aspects</topic><topic>Health aspects</topic><topic>Humans</topic><topic>Hypersensitivity, Immediate - blood</topic><topic>Hypersensitivity, Immediate - genetics</topic><topic>Immunoglobulin E - biosynthesis</topic><topic>Interleukin-4</topic><topic>Interleukin-4 - biosynthesis</topic><topic>Leukocytes - physiology</topic><topic>Male</topic><topic>Measurement</topic><topic>Middle Aged</topic><topic>Oxygenases - metabolism</topic><topic>Physiological aspects</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Research Article</topic><topic>Rhinitis, Allergic - blood</topic><topic>Rhinitis, Allergic - genetics</topic><topic>Up-Regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Marbach-Breitrück, Eugenia</creatorcontrib><creatorcontrib>Kalledat, Andrea</creatorcontrib><creatorcontrib>Heydeck, Dagmar</creatorcontrib><creatorcontrib>Stehling, Sabine</creatorcontrib><creatorcontrib>Fluhr, Joachim W.</creatorcontrib><creatorcontrib>Zuberbier, Torsten</creatorcontrib><creatorcontrib>Kuhn, Hartmut</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Skin pharmacology and physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Marbach-Breitrück, Eugenia</au><au>Kalledat, Andrea</au><au>Heydeck, Dagmar</au><au>Stehling, Sabine</au><au>Fluhr, Joachim W.</au><au>Zuberbier, Torsten</au><au>Kuhn, Hartmut</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Atopic Patients Show Increased Interleukin 4 Plasma Levels but the Degree of Elevation Is Not Sufficient to Upregulate Interleukin-4-Sensitive Genes</atitle><jtitle>Skin pharmacology and physiology</jtitle><addtitle>Skin Pharmacol Physiol</addtitle><date>2019-07-01</date><risdate>2019</risdate><volume>32</volume><issue>4</issue><spage>192</spage><epage>200</epage><pages>192-200</pages><issn>1660-5527</issn><eissn>1660-5535</eissn><abstract>Background: Atopic diseases constitute a major health challenge for industrialized countries, and elevated levels of interleukin 4 (IL-4) frequently characterize these disorders. Previous in vitroanalyses have indicated that IL-4 strongly upregulates the expression of IL-4-sensitive genes in human monocytes. Objective: To explore whether similar expression alterations may contribute to the pathomechanisms of atopic diseases in vivo we carried out a small-scale case-control clinical study (n = 43), in which we quantified the plasma levels of IgE and IL-4 as well as the expression of selected IL-4-sensitive genes in blood leukocytes. Methods: 34 allergic patients suffering from allergic rhinitis (n = 11), atopic eczema (n = 11) and allergic asthma (n = 12) as well as 9 healthy control individuals were recruited. IgE and IL-4 plasma levels were determined by ELISA, and the expression of selected IL-4-sensitive gene products in blood leukocytes was quantified by qRT-PCR. In addition, the fatty acid oxygenase activity of isolated monocytes was measured by RP-HPLC analysis of the arachidonic acid oxygenation products (ex vivo activity assays). Results: We found that plasma levels of IgE and IL-4 were significantly elevated in atopic patients but the degree of elevation was not sufficient to upregulate the expression of the selected IL-4-sensitive genes in circulating leukocytes. Moreover, the arachidonic acid oxygenase activity of blood monocytes was not significantly altered in atopic patients. Conclusion: Our data suggest that the IL-4 plasma levels of atopic patients are not high enough to impact the expression of IL-4-sensitive genes.</abstract><cop>Basel, Switzerland</cop><pub>S. Karger AG</pub><pmid>31096247</pmid><doi>10.1159/000499431</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-1466-8875</orcidid><orcidid>https://orcid.org/0000-0003-3610-0698</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Skin pharmacology and physiology, 2019-07, Vol.32 (4), p.192-200 |
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| source | MEDLINE; Karger Journals; Alma/SFX Local Collection |
| subjects | Adult Asthma - blood Asthma - genetics Atopic dermatitis Case-Control Studies Dermatitis, Atopic - blood Dermatitis, Atopic - genetics Development and progression Enzyme-Linked Immunosorbent Assay Female Gene expression Genetic aspects Health aspects Humans Hypersensitivity, Immediate - blood Hypersensitivity, Immediate - genetics Immunoglobulin E - biosynthesis Interleukin-4 Interleukin-4 - biosynthesis Leukocytes - physiology Male Measurement Middle Aged Oxygenases - metabolism Physiological aspects Real-Time Polymerase Chain Reaction Research Article Rhinitis, Allergic - blood Rhinitis, Allergic - genetics Up-Regulation |
| title | Atopic Patients Show Increased Interleukin 4 Plasma Levels but the Degree of Elevation Is Not Sufficient to Upregulate Interleukin-4-Sensitive Genes |
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